##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528161_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2002693
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.292826209508895	31.0	31.0	33.0	27.0	33.0
2	31.297444490992877	31.0	31.0	34.0	28.0	34.0
3	31.734917433675555	31.0	31.0	34.0	30.0	34.0
4	35.60687184705794	37.0	35.0	37.0	33.0	37.0
5	35.65930125086571	37.0	35.0	37.0	33.0	37.0
6	35.85648224665488	37.0	35.0	37.0	35.0	37.0
7	35.77813873619172	37.0	35.0	37.0	35.0	37.0
8	35.505114363509534	37.0	35.0	37.0	33.0	37.0
9	37.080465652998235	39.0	37.0	39.0	33.0	39.0
10	33.94114424926836	35.0	32.0	37.0	28.0	39.0
11	36.6542106054198	37.0	35.0	39.0	34.0	39.0
12	37.4868100103211	39.0	37.0	39.0	35.0	39.0
13	37.530126185091774	39.0	37.0	39.0	35.0	39.0
14	37.76602904189509	40.0	38.0	40.0	33.0	40.0
15	36.94390553120223	39.0	36.0	40.0	31.0	40.0
16	36.85573974643143	38.0	36.0	40.0	31.0	40.0
17	36.73506872995512	39.0	36.0	40.0	31.0	40.0
18	36.75723288591911	39.0	36.0	40.0	31.0	40.0
19	36.92046259711299	39.0	36.0	40.0	32.0	40.0
20	36.99943526042184	39.0	36.0	40.0	32.0	40.0
21	36.99397760914928	39.0	36.0	40.0	32.0	40.0
22	37.093559022775835	39.0	36.0	40.0	32.0	40.0
23	37.044605438776685	39.0	36.0	40.0	32.0	40.0
24	37.12970185645029	39.0	36.0	40.0	32.0	40.0
25	37.24855581958892	39.0	36.0	40.0	32.0	40.0
26	37.074194596975175	39.0	36.0	40.0	32.0	40.0
27	36.84769857387028	39.0	36.0	40.0	31.0	40.0
28	36.690677003414905	39.0	36.0	40.0	31.0	40.0
29	36.65707424952302	39.0	35.0	40.0	31.0	40.0
30	36.59243928050879	39.0	35.0	40.0	31.0	40.0
31	36.526227934086755	39.0	35.0	40.0	31.0	40.0
32	36.5017838480486	39.0	35.0	40.0	31.0	40.0
33	36.353627340785636	39.0	35.0	40.0	31.0	40.0
34	36.1226813096166	38.0	35.0	40.0	30.0	40.0
35	36.02407957684977	38.0	35.0	40.0	30.0	40.0
36	35.81508548739123	38.0	35.0	40.0	30.0	40.0
37	35.580807942105956	38.0	35.0	40.0	29.0	40.0
38	35.20391093392747	38.0	34.0	40.0	27.0	40.0
39	34.95395599824836	37.0	34.0	40.0	26.0	40.0
40	34.67346268249802	37.0	34.0	40.0	25.0	40.0
41	34.30228297597285	36.0	33.0	40.0	24.0	40.0
42	33.95268071541669	36.0	33.0	40.0	23.0	40.0
43	33.56262392688245	35.0	33.0	40.0	21.0	40.0
44	33.17539882548149	35.0	33.0	40.0	18.0	40.0
45	32.8525879902711	35.0	33.0	40.0	17.0	40.0
46	32.37491867200814	35.0	32.0	39.0	14.0	40.0
47	31.90531699067206	35.0	31.0	39.0	10.0	40.0
48	31.505462894212943	35.0	30.0	39.0	10.0	40.0
49	31.18040408589834	35.0	29.0	39.0	9.0	40.0
50	30.763728639387065	35.0	27.0	39.0	9.0	40.0
51	30.32527152189577	35.0	27.0	38.0	9.0	40.0
52	29.362568801109305	35.0	23.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	0.0
14	3.0
15	15.0
16	69.0
17	216.0
18	573.0
19	1107.0
20	2265.0
21	3981.0
22	6281.0
23	9613.0
24	13946.0
25	19895.0
26	27139.0
27	35832.0
28	47530.0
29	61164.0
30	74591.0
31	84328.0
32	93275.0
33	109735.0
34	144169.0
35	190428.0
36	238158.0
37	346739.0
38	419265.0
39	72374.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.27244864789561	21.188319927218004	37.64301368207708	7.8962177428093066
2	26.21649948344554	20.971312128219353	36.55233228457882	16.25985610375629
3	21.976059236238406	20.65024444585366	41.64822067086668	15.725475647041259
4	25.19702220959478	18.189258163882332	40.30757584911916	16.306143777403726
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	97.98770954909214	0.4802034061136679	1.4243321367778288	0.10775490801635597
12	98.71947422795206	0.883710084371394	0.22559623467001683	0.17121945300652672
13	94.2914865134097	3.663866603618228	1.146805825955351	0.897841057016727
14	47.3954819835092	32.38194770741197	11.758317425586448	8.464252883492378
15	28.04234098785985	37.53271220301863	21.56181701339147	12.86312979573005
16	38.59727876414408	23.77293973664461	21.855771204073715	15.774010295137597
17	27.878162054793222	23.52332584175408	21.755955605776823	26.84255649767588
18	20.141379632325073	35.998328251010015	25.84999298444644	18.010299132218467
19	25.4374984083931	32.38958742053825	29.3374471274429	12.835467043625759
20	20.873693571605834	34.70646774118649	27.932389038160117	16.48744964904756
21	26.286605086251363	34.76004559860149	18.057984923300776	20.89536439184638
22	25.795216740658706	34.512828476456455	18.65752763903404	21.034427143850806
23	19.537342967694	37.04132385742598	26.243513109597927	17.1778200652821
24	22.80174744706253	39.30787195041876	16.935346555862534	20.955034046656177
25	22.216086040146944	40.00263645002005	20.16185206619287	17.61942544364014
26	21.720353543953067	36.30057128077044	21.681555785135316	20.297519390141176
27	24.74667859726878	39.39630287817454	16.9901727324158	18.866845792140882
28	21.733835390646494	38.87096025202065	15.054029748943048	24.341174608389803
29	22.814680033335115	42.78159458289414	15.347185015376796	19.056540368393957
30	23.921240050272306	41.937730845416645	18.688935348553173	15.452093755757874
31	19.37665932821456	44.54237369382127	15.1014658761977	20.97950110176647
32	20.730037005172537	50.30990770926947	15.13117587168877	13.828879413869224
33	22.554929787041747	44.39472250614547	15.023920291327727	18.02642741548505
34	21.204098681125863	46.5066288242881	15.464477081609612	16.824795412976425
35	21.405028129623464	47.93535504443267	16.355826879107283	14.303789946836584
36	20.71056322661536	45.271791532701215	21.16280428403155	12.85484095665187
37	21.060392181927035	46.54352913801566	16.962310249249384	15.433768430807918
38	16.952473494439737	50.66253289945089	20.490010201264	11.894983404845377
39	17.831340100554605	48.07316947729882	21.154715176015497	12.940775246131084
40	19.086549960478216	46.534141778095794	16.628110249548982	17.751198011877005
41	20.481921093247944	49.150219229807064	14.156138759160791	16.211720917784202
42	18.85635991137933	49.09818928812354	14.345134276696427	17.7003165238007
43	19.2053899424425	47.384646573388935	16.156944673996463	17.2530188101721
44	20.08380715366759	47.21063088551266	17.14915865786718	15.556403302952573
45	22.261175327421626	42.59000256155087	20.559966005773227	14.588856105254274
46	24.165361340954405	42.91731183960797	17.781557133319982	15.135769686117643
47	22.815528890349146	42.278322239105044	17.321776228308583	17.584372642237227
48	23.01715739756418	41.87950924080725	18.17223109083619	16.93110227079238
49	22.1331477165996	44.694418964863814	15.739307023093405	17.433126295443184
50	19.836140636632773	45.79034330274286	16.645087389829595	17.728428670794774
51	19.370467665288686	45.47566701436516	19.651239605870696	15.50262571447546
52	24.54779639215796	42.25919798990659	17.760135976907094	15.432869641028354
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	22.0
6	43.0
7	57.5
8	72.0
9	839.0
10	1606.0
11	3070.5
12	4535.0
13	7766.5
14	12184.5
15	13371.0
16	12215.0
17	11059.0
18	13334.0
19	15609.0
20	21464.5
21	27320.0
22	38635.5
23	49951.0
24	66296.5
25	82642.0
26	95199.5
27	107757.0
28	105590.0
29	103423.0
30	91300.0
31	79177.0
32	72320.5
33	65464.0
34	63262.0
35	61060.0
36	60633.5
37	60207.0
38	61789.5
39	62034.5
40	60697.0
41	69706.0
42	78715.0
43	91405.5
44	104096.0
45	119850.0
46	135604.0
47	150479.0
48	165354.0
49	158748.0
50	152142.0
51	147910.5
52	143679.0
53	123533.0
54	103387.0
55	89082.0
56	74777.0
57	66912.5
58	59048.0
59	62849.0
60	66650.0
61	52753.5
62	38857.0
63	33741.5
64	20611.5
65	12597.0
66	11201.0
67	9805.0
68	7261.0
69	4717.0
70	4014.0
71	3311.0
72	2956.5
73	2602.0
74	1404.0
75	206.0
76	120.5
77	35.0
78	28.0
79	21.0
80	20.0
81	19.0
82	14.0
83	9.0
84	7.5
85	6.0
86	35.0
87	64.0
88	33.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2002693.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.761522957879393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.21423456005247	25.290350638835356
2	7.639047632205655	4.69977478228857
3	2.6718632757763587	2.465717504943279
4	1.3660412070397798	1.6808603180705386
5	0.8794549302997218	1.3526686514417559
6	0.6105251288923306	1.1268409661270224
7	0.45773746586968594	0.9856491095522316
8	0.37577425145138466	0.924751061040137
9	0.3021028721603408	0.8363829993841465
>10	2.8663161382396494	18.639396988078584
>50	0.3390674973071355	7.077994226304164
>100	0.20704695860348288	13.371792086598989
>500	0.0453641934597889	9.89941474842483
>1k	0.025423888642299275	11.648405918910482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	4522	0.2257959657321417	No Hit
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGG	3896	0.19453805450960282	No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGAC	3274	0.16347987434918881	No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGG	3062	0.15289412805657182	No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCC	2837	0.14165925581204908	No Hit
GTTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	2643	0.1319722992989939	No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGG	2476	0.12363352745528146	No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATT	2440	0.12183594789615783	No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATC	2403	0.11998843557150297	No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATG	2367	0.11819085601237932	No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTC	2362	0.11794119218472326	No Hit
AATCCAAAGTGGGAGACGCCGCCTCGGCGAGCCGGGCGCCGGGCAGGAACGC	2330	0.11634334368772449	No Hit
TGCTCAAAGTTGGGGTTGTGGCAATAGCCCAGTATGCAGTCACTTAGAAATT	2310	0.11534468837710024	No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATC	2294	0.11454576412860083	No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2290	0.11434603306647599	No Hit
AGCTCAAAGTGGACAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCC	2217	0.11070094118269751	No Hit
TGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	2144	0.10705584929891901	No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAA	2118	0.10575759739510748	No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG	2114	0.10555786633298263	No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG	2093	0.10450927825682718	No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA	2080	0.10386015230492143	No Hit
CTGACAAAGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATG	2077	0.1037103540083278	No Hit
TTAGCAAAGTGGGGCAGTGGTATCAACGCAGCTACCTCTCCCCACGGGTGCA	2071	0.10341075741514051	No Hit
AGTTCAAAGTGGGGGCTGGAGAGGTGGCTCAGAGGTTTAGAGCACTGGCTGT	2021	0.1009141191385799	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.05512577314645829	0.0	0.0	0.0	0.0
12	0.12113688917872086	0.0	0.0	0.0	0.0
13	0.17121945300652672	0.0	0.0	0.0	0.0
14	0.6126251003024428	0.0	0.0	0.0	0.0
15	1.1046126390814768	0.0	0.0	0.0	0.0
16	1.3093369777594468	0.0	0.0	0.0	0.0
17	1.4444550412869073	0.0	0.0	0.0	0.0
18	1.6298553997042982	0.0	0.0	0.0	0.0
19	1.7805025533119654	0.0	0.0	0.0	0.0
20	1.965054054715326	0.0	0.0	0.0	0.0
21	2.194994439986558	0.0	0.0	0.0	0.0
22	2.3898320910893482	0.0	0.0	0.0	0.0
23	2.570638635077868	0.0	0.0	0.0	0.0
24	2.7550403381846342	0.0	0.0	0.0	0.0
25	2.9628605083255395	0.0	0.0	0.0	0.0
26	3.209029042394416	0.0	0.0	0.0	0.0
27	3.4495551739582653	0.0	0.0	0.0	0.0
28	3.6828410545200887	0.0	0.0	0.0	0.0
29	4.018439171655366	0.0	0.0	0.0	0.0
30	4.400324962438077	0.0	0.0	0.0	0.0
31	4.706562613441002	0.0	0.0	0.0	0.0
32	4.9581238861872485	0.0	0.0	0.0	0.0
33	5.141726665045516	0.0	0.0	0.0	0.0
34	5.355139304925917	0.0	0.0	0.0	0.0
35	5.553322451319299	0.0	0.0	0.0	0.0
36	5.760443562742767	0.0	0.0	0.0	0.0
37	5.97066050562917	0.0	0.0	0.0	0.0
38	6.171839617954424	0.0	0.0	0.0	0.0
39	6.375115906431989	0.0	0.0	0.0	0.0
40	6.585981975270299	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGAATTT	150	0.0	46.000004	12
CACATTT	75	0.0	46.000004	19
TTAGATA	155	0.0	46.000004	31
GATCCCC	150	0.0	46.000004	13
TTCACGA	75	0.0	46.000004	20
CCGTGTC	145	0.0	46.000004	33
TTCACCG	150	0.0	46.000004	25
CAACCGT	150	0.0	46.000004	45
CCGTGAA	145	0.0	46.000004	34
TGAACCT	155	0.0	46.000004	21
ATGCTTA	75	0.0	46.000004	44
ATTAAGG	75	0.0	46.000004	44
TTCGTGT	75	0.0	46.000004	21
CTAAGTC	145	0.0	46.000004	19
GTTAGAA	75	0.0	46.000004	33
TAAGTCC	145	0.0	46.000004	20
ATGTCTA	75	0.0	46.000004	22
GTAATCT	150	0.0	46.000004	37
ATGTAAC	150	0.0	46.000004	44
TCTACGG	75	0.0	46.000004	17
>>END_MODULE