FastQCFastQC Report
Fri 17 Jun 2016
SRR1528160_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528160_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1989648
Sequences flagged as poor quality0
Sequence length52
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG44970.22601987889315098No Hit
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGG39010.19606483156819698No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGAC33910.17043215684382362No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGG29730.14942341559914116No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCC28700.1442466205077481No Hit
GTTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG27020.13580291589266041No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATC24260.12193111545358776No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATG23580.11851342549033798No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTC23500.11811134431819095No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATT23460.11791030373211744No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC23280.11700562109478663No Hit
AATCCAAAGTGGGAGACGCCGCCTCGGCGAGCCGGGCGCCGGGCAGGAACGC23180.11650301962960283No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGG23030.11574911743182713No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATC22270.11192934629643034No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG22210.11162778541732005No Hit
TGCTCAAAGTTGGGGTTGTGGCAATAGCCCAGTATGCAGTCACTTAGAAATT22200.11157752527080167No Hit
TGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG22200.11157752527080167No Hit
AGCTCAAAGTGGACAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCC21600.10856191647969891No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA20900.10504370622341239No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAA20890.10499344607689401No Hit
TTTTCAAAGTGGGGAAACAAACAAAGCCTCCAAGAAATATGGGACTATGTGA20710.10408876343956318No Hit
CTGACAAAGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATG20200.10152549596712585No Hit
GTAACAAAGTGAGGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGA20140.10122393508801557No Hit
AGTTCAAAGTGGGGGCTGGAGAGGTGGCTCAGAGGTTTAGAGCACTGGCTGT20060.10082185391586854No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG20040.10072133362283178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCACTA700.046.00000443
CCTTAAG351.0200711E-746.00000441
CCGGGCC1550.046.00000428
TACTTTG1400.046.00000427
GTCTGTA351.0200711E-746.00000424
CTCCGTA750.046.00000435
GTACAGT351.0200711E-746.00000416
TGCCATT700.046.00000422
TTTACAC351.0200711E-746.00000424
CCCTAGT351.0200711E-746.00000423
GACGTTG351.0200711E-746.00000444
TTAGATA1400.046.00000431
TAGGTGC351.0200711E-746.00000410
GACAATT1400.046.00000433
ATAGCTA1550.046.00000446
CCGTGTC1450.046.00000433
TTCACCG1450.046.00000425
CCAATCC351.0200711E-746.00000418
AGGACGT351.0200711E-746.00000419
GAATCAC700.046.00000427
Produced by FastQC (version 0.11.5)