##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528160_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1989648
Sequences flagged as poor quality	0
Sequence length	52
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.31786929145256	31.0	31.0	33.0	27.0	33.0
2	31.31777429977564	31.0	31.0	34.0	30.0	34.0
3	31.755181821106046	31.0	31.0	34.0	30.0	34.0
4	35.63806210947866	37.0	35.0	37.0	33.0	37.0
5	35.77203455083512	37.0	35.0	37.0	35.0	37.0
6	35.89312782964625	37.0	35.0	37.0	35.0	37.0
7	35.76839672143012	37.0	35.0	37.0	35.0	37.0
8	35.49531826735181	37.0	35.0	37.0	33.0	37.0
9	37.06791502818589	39.0	37.0	39.0	33.0	39.0
10	33.88806210947866	35.0	32.0	37.0	28.0	39.0
11	36.64438031249749	37.0	35.0	39.0	34.0	39.0
12	37.50559998552508	39.0	37.0	39.0	35.0	39.0
13	37.529206673743296	39.0	37.0	39.0	35.0	39.0
14	37.71807324712713	40.0	38.0	40.0	33.0	40.0
15	36.93835995110693	39.0	36.0	40.0	31.0	40.0
16	36.84104525021511	38.0	36.0	40.0	31.0	40.0
17	36.74010779796225	39.0	36.0	40.0	31.0	40.0
18	36.6708121235515	39.0	36.0	40.0	31.0	40.0
19	36.91113604014378	39.0	36.0	40.0	32.0	40.0
20	36.999165681567796	39.0	36.0	40.0	32.0	40.0
21	37.04101026915313	39.0	36.0	40.0	32.0	40.0
22	37.09237412848906	39.0	36.0	40.0	32.0	40.0
23	37.14363847273488	39.0	36.0	40.0	32.0	40.0
24	37.26243838106037	39.0	36.0	40.0	32.0	40.0
25	37.29180940548278	39.0	36.0	40.0	32.0	40.0
26	36.97759503188504	39.0	36.0	40.0	31.0	40.0
27	36.722119691523325	39.0	36.0	40.0	31.0	40.0
28	36.608284480470914	39.0	35.0	40.0	30.0	40.0
29	36.571929306088315	39.0	35.0	40.0	31.0	40.0
30	36.39518698784911	39.0	35.0	40.0	30.0	40.0
31	36.38482636124581	39.0	35.0	40.0	30.0	40.0
32	36.451225543412704	39.0	35.0	40.0	30.0	40.0
33	36.40503797656671	39.0	35.0	40.0	31.0	40.0
34	36.2226253085973	38.0	35.0	40.0	30.0	40.0
35	35.94919955690655	38.0	35.0	40.0	30.0	40.0
36	35.78516903492477	38.0	35.0	40.0	30.0	40.0
37	35.55298927247433	38.0	35.0	40.0	29.0	40.0
38	35.23578190715141	38.0	34.0	40.0	27.0	40.0
39	34.987180144427555	37.0	34.0	40.0	27.0	40.0
40	34.647711555008726	37.0	34.0	40.0	25.0	40.0
41	34.255013449615205	36.0	33.0	40.0	24.0	40.0
42	33.901903251228354	36.0	33.0	40.0	22.0	40.0
43	33.54888000289498	35.0	33.0	40.0	21.0	40.0
44	33.073867337338065	35.0	33.0	40.0	17.0	40.0
45	32.742107146590754	35.0	33.0	40.0	15.0	40.0
46	32.255300937653296	35.0	31.0	39.0	14.0	40.0
47	31.919699363907586	35.0	31.0	39.0	10.0	40.0
48	31.519225008644746	35.0	30.0	39.0	10.0	40.0
49	31.010012323787926	35.0	28.0	39.0	9.0	40.0
50	30.596970921489632	35.0	27.0	38.0	9.0	40.0
51	30.172384260934596	35.0	26.0	38.0	9.0	40.0
52	28.993084706440538	34.0	23.0	37.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	1.0
13	2.0
14	7.0
15	30.0
16	71.0
17	198.0
18	538.0
19	1176.0
20	2264.0
21	3896.0
22	6356.0
23	9678.0
24	14020.0
25	20224.0
26	27403.0
27	36387.0
28	47481.0
29	61137.0
30	75071.0
31	84509.0
32	92867.0
33	110297.0
34	142946.0
35	189465.0
36	237105.0
37	347337.0
38	415728.0
39	63453.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.32056725611767	21.196714192661215	37.59966587054595	7.883052680675175
2	26.21860751248462	20.95129389721197	36.533095301279424	16.297003289023987
3	21.921616285895798	20.639680988798016	41.692450121830596	15.746252603475588
4	25.18641488343667	18.149843590424034	40.41262575088659	16.251115775252707
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	98.00331515926435	0.484407292144138	1.3981870159947891	0.11409053259672064
12	98.750231196674	0.8613081308854631	0.22310479039508493	0.16535588204546733
13	94.33186171624327	3.646624930640998	1.132913962670784	0.8885993904449431
14	47.28318777994902	32.4879576688942	11.779470539512516	8.44938401164427
15	28.00937653293447	37.63675785867651	21.48510691338367	12.868758695005347
16	38.65005267263355	23.773551904658515	21.86261087388322	15.713784548824716
17	27.811954677410277	23.461587175218934	21.807023151833892	26.9194349955369
18	20.075510844129212	36.0872878016614	25.774106776676074	18.063094577533313
19	25.455105626723924	32.41407525351218	29.317547626514838	12.813271493249056
20	20.812575892821243	34.77851358632281	27.946249788907384	16.462660731948567
21	26.29510345548559	34.79866790507668	18.055203734529925	20.851024904907803
22	25.814767235209445	34.474389439740094	18.683355045716628	21.02748827933383
23	19.470981801806147	37.059721116499	26.35109325870707	17.118203822987784
24	22.833636904618302	39.4060155364165	16.81724606563573	20.943101493329475
25	22.15532596720626	40.001698792952325	20.195733114601175	17.647242125240243
26	21.59246258634693	36.35643088626732	21.70780962260661	20.343296904779137
27	24.68954307495597	39.40445747187442	17.01607520526244	18.889924247907167
28	21.63528423118059	38.96040907738455	14.967672673759377	24.436634017675487
29	22.767544811946635	42.82089093146124	15.291448537630778	19.120115718961344
30	23.85889363344672	42.01310985661785	18.72672955216199	15.401266957773435
31	19.260542568333694	44.62729085747831	15.099253737344496	21.012912836843505
32	20.657975682130708	50.52944038342461	15.042861852950873	13.769722081493812
33	22.569168013638592	44.56366151198604	14.847249362701342	18.019921111674027
34	21.196211591196032	46.672929080922856	15.376840526565502	16.75401880131561
35	21.28436788818927	47.97888872805642	16.466983104549147	14.269760279205165
36	20.686221884474037	45.340934677892776	21.166809405482777	12.806034032150409
37	21.096847281529197	46.59276414722604	16.874793933399275	15.435594637845487
38	16.953300282260983	50.757822489204116	20.464172557155837	11.824704671379058
39	17.866074803181267	48.18123607793942	21.086342910906854	12.866346207972464
40	19.01693163815911	46.657097134769565	16.57257967238426	17.75339155468706
41	20.482014909169862	49.327117158411944	14.061230931300411	16.129637001117786
42	18.823329553770314	49.31977917702026	14.241916158033984	17.614975111175443
43	19.222344856979728	47.50704647254187	16.06248944536923	17.208119225109165
44	20.012786181274276	47.34938039291372	17.113981970680243	15.523851455131762
45	22.250016083246884	42.67694587183261	20.52408265180575	14.548955393114763
46	24.152714449993166	43.005848270648876	17.741178339083095	15.100258940274863
47	22.88751578168601	42.400314025395446	17.14353493683305	17.5686352560855
48	22.977179883074793	42.06824523734852	18.084103318777995	16.870471560798695
49	22.08641930632956	44.659758912129185	15.887282574606163	17.366539206935098
50	19.825265574614203	45.891233022122506	16.602936800881363	17.680564602381928
51	19.31653237155517	45.53895965517519	19.682577018648526	15.46193095462112
52	24.674263990414385	42.17605325163044	17.762840462232514	15.38684229572266
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	22.5
6	41.0
7	62.5
8	84.0
9	817.5
10	1551.0
11	3001.0
12	4451.0
13	7652.0
14	12029.0
15	13205.0
16	12110.0
17	11015.0
18	13350.0
19	15685.0
20	21240.5
21	26796.0
22	38202.0
23	49608.0
24	65853.5
25	82099.0
26	94779.5
27	107460.0
28	105542.0
29	103624.0
30	91316.5
31	79009.0
32	72523.0
33	66037.0
34	63460.0
35	60883.0
36	60388.5
37	59894.0
38	61718.0
39	61757.0
40	59972.0
41	69241.0
42	78510.0
43	91072.5
44	103635.0
45	119352.5
46	135070.0
47	149900.5
48	164731.0
49	157608.5
50	150486.0
51	146085.5
52	141685.0
53	121782.5
54	101880.0
55	87856.0
56	73832.0
57	65977.5
58	58123.0
59	61857.5
60	65592.0
61	52307.0
62	39022.0
63	33730.5
64	20473.5
65	12508.0
66	10964.5
67	9421.0
68	7028.0
69	4635.0
70	3993.5
71	3352.0
72	2979.5
73	2607.0
74	1397.0
75	187.0
76	109.0
77	31.0
78	25.0
79	19.0
80	17.0
81	15.0
82	8.5
83	2.0
84	4.5
85	7.0
86	25.5
87	44.0
88	23.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1989648.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.31098727484951
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.47340074562318	25.82323601259772
2	7.576870383526292	4.744785843235517
3	2.6361096765875986	2.476175896162259
4	1.3503059802080224	1.6911765345378633
5	0.8408149189724313	1.316337261422471
6	0.6081363626462827	1.1424809947314616
7	0.4463797730425893	0.978361397544071
8	0.36038925863695803	0.9027314792939395
9	0.296789347496012	0.836349073448267
>10	2.812452367110781	18.666371864519423
>50	0.32779964665319455	6.9884852842199985
>100	0.20143974392848105	13.18753767284799
>500	0.04536485159342367	10.11568577042577
>1k	0.023746943974896868	11.130284915013334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	4497	0.22601987889315098	No Hit
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGG	3901	0.19606483156819698	No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGAC	3391	0.17043215684382362	No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGG	2973	0.14942341559914116	No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCC	2870	0.1442466205077481	No Hit
GTTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	2702	0.13580291589266041	No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATC	2426	0.12193111545358776	No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATG	2358	0.11851342549033798	No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTC	2350	0.11811134431819095	No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATT	2346	0.11791030373211744	No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAAC	2328	0.11700562109478663	No Hit
AATCCAAAGTGGGAGACGCCGCCTCGGCGAGCCGGGCGCCGGGCAGGAACGC	2318	0.11650301962960283	No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGG	2303	0.11574911743182713	No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATC	2227	0.11192934629643034	No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG	2221	0.11162778541732005	No Hit
TGCTCAAAGTTGGGGTTGTGGCAATAGCCCAGTATGCAGTCACTTAGAAATT	2220	0.11157752527080167	No Hit
TGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTG	2220	0.11157752527080167	No Hit
AGCTCAAAGTGGACAGCGGGTGTTGGCGGGTGTCGGGGCGCAGCCATGACCC	2160	0.10856191647969891	No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAA	2090	0.10504370622341239	No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAA	2089	0.10499344607689401	No Hit
TTTTCAAAGTGGGGAAACAAACAAAGCCTCCAAGAAATATGGGACTATGTGA	2071	0.10408876343956318	No Hit
CTGACAAAGTGGGGCTTCCTGTCCCGGCCGCCATCGGAGAGCATCAGCCATG	2020	0.10152549596712585	No Hit
GTAACAAAGTGAGGGAGAATCGCTTGAACCCGGGAGGTGGAGGTTGCAGTGA	2014	0.10122393508801557	No Hit
AGTTCAAAGTGGGGGCTGGAGAGGTGGCTCAGAGGTTTAGAGCACTGGCTGT	2006	0.10082185391586854	No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTG	2004	0.10072133362283178	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.056341624247103005	0.0	0.0	0.0	0.0
12	0.11997096973937098	0.0	0.0	0.0	0.0
13	0.1741011475396653	0.0	0.0	0.0	0.0
14	0.6064891880372809	0.0	0.0	0.0	0.0
15	1.0857699452365444	0.0	0.0	0.0	0.0
16	1.2835938819328847	0.0	0.0	0.0	0.0
17	1.4076359235402442	0.0	0.0	0.0	0.0
18	1.5903315561345523	0.0	0.0	0.0	0.0
19	1.7393528905615465	0.0	0.0	0.0	0.0
20	1.919686296269491	0.0	0.0	0.0	0.0
21	2.1492243854189286	0.0	0.0	0.0	0.0
22	2.342173087902986	0.0	0.0	0.0	0.0
23	2.5164752760287246	0.0	0.0	0.0	0.0
24	2.694949056315489	0.0	0.0	0.0	0.0
25	2.9040815259784645	0.0	0.0	0.0	0.0
26	3.14020369432181	0.0	0.0	0.0	0.0
27	3.3826083809799523	0.0	0.0	0.0	0.0
28	3.6184792485907056	0.0	0.0	0.0	0.0
29	3.9416017305573647	0.0	0.0	0.0	0.0
30	4.323578844097046	0.0	0.0	0.0	0.0
31	4.6350912322179605	0.0	0.0	0.0	0.0
32	4.897449197043899	0.0	0.0	0.0	0.0
33	5.090096338648847	0.0	0.0	0.0	0.0
34	5.304003522231068	0.0	0.0	0.0	0.0
35	5.496097802224313	0.0	0.0	0.0	0.0
36	5.701008419579745	0.0	0.0	0.0	0.0
37	5.90240082667889	0.0	0.0	0.0	0.0
38	6.1006771046938955	0.0	0.0	0.0	0.0
39	6.299405724027567	0.0	0.0	0.0	0.0
40	6.505019983434256	0.0	5.026014651837913E-5	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCACTA	70	0.0	46.000004	43
CCTTAAG	35	1.0200711E-7	46.000004	41
CCGGGCC	155	0.0	46.000004	28
TACTTTG	140	0.0	46.000004	27
GTCTGTA	35	1.0200711E-7	46.000004	24
CTCCGTA	75	0.0	46.000004	35
GTACAGT	35	1.0200711E-7	46.000004	16
TGCCATT	70	0.0	46.000004	22
TTTACAC	35	1.0200711E-7	46.000004	24
CCCTAGT	35	1.0200711E-7	46.000004	23
GACGTTG	35	1.0200711E-7	46.000004	44
TTAGATA	140	0.0	46.000004	31
TAGGTGC	35	1.0200711E-7	46.000004	10
GACAATT	140	0.0	46.000004	33
ATAGCTA	155	0.0	46.000004	46
CCGTGTC	145	0.0	46.000004	33
TTCACCG	145	0.0	46.000004	25
CCAATCC	35	1.0200711E-7	46.000004	18
AGGACGT	35	1.0200711E-7	46.000004	19
GAATCAC	70	0.0	46.000004	27
>>END_MODULE