FastQCFastQC Report
Fri 17 Jun 2016
SRR1528159_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528159_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1891052
Sequences flagged as poor quality0
Sequence length59
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGGCACTAGC31650.16736715859743675No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGACTGCTCTT30880.16329535094751493No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCCATATTCC25470.13468693616040173No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGGGAGGCCG23950.12664908209821835No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGGCTGAGGC23820.12596163405342634No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATGACGTTAC22710.12009188536327928No Hit
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGGTATGAA22520.11908715360550634No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATTACTTAGG22330.11808242184773343No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG21370.11300588244003867No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTT20360.10766493993819312No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTCTAGCTCT20250.10708325313106146No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA19400.10258840053049836No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATCAACTCGT19370.1024297586740079No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATCGCCCAAA19370.1024297586740079No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT19280.10195383310453651No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAATAAGCCT19070.1008433401091033No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTAAC203.1387628E-453.00000416
AGGTACT457.6397555E-1153.00000439
GTTTCTT203.1387628E-453.00000433
TACTTAC457.6397555E-1153.00000431
TAGACTT203.1387628E-453.00000436
TTTAACG1550.053.00000449
CATGACT900.053.00000445
ATTACTC203.1387628E-453.00000444
TTCACGA800.053.00000420
ACATTAG1500.053.00000435
CCTACAC1600.053.00000432
TAAGTTA203.1387628E-453.00000437
ATTCGGC203.1387628E-453.00000428
TAAGTGT900.053.00000414
TAACGTT1600.053.00000451
CTAAGCT203.1387628E-453.00000431
CACTGAA203.1387628E-453.00000446
CGACACC203.1387628E-453.00000416
TACATGT203.1387628E-453.00000431
TATTGGC900.053.00000425