##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528159_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1891052
Sequences flagged as poor quality	0
Sequence length	59
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.959162413302227	31.0	30.0	31.0	27.0	33.0
2	30.932159982908985	31.0	31.0	33.0	28.0	34.0
3	31.3976331692624	31.0	31.0	34.0	30.0	34.0
4	35.36313755518093	37.0	35.0	37.0	33.0	37.0
5	35.36009586198581	37.0	35.0	37.0	33.0	37.0
6	35.581692623999764	37.0	35.0	37.0	33.0	37.0
7	35.382050837311716	37.0	35.0	37.0	33.0	37.0
8	35.08119871901989	37.0	35.0	37.0	32.0	37.0
9	36.698124641733806	39.0	35.0	39.0	32.0	39.0
10	33.24394516914395	35.0	32.0	37.0	27.0	39.0
11	36.007437130232276	37.0	35.0	39.0	32.0	39.0
12	37.02978024930039	39.0	37.0	39.0	34.0	39.0
13	37.13389637090889	39.0	37.0	39.0	34.0	39.0
14	36.94127871682006	39.0	36.0	40.0	31.0	40.0
15	36.11736324543164	38.0	35.0	40.0	30.0	40.0
16	35.860604044732774	38.0	34.0	40.0	30.0	40.0
17	35.96582801530577	38.0	35.0	40.0	30.0	40.0
18	36.0387297652312	38.0	35.0	40.0	30.0	40.0
19	36.3498941330011	38.0	35.0	40.0	30.0	40.0
20	36.3158199774517	38.0	35.0	40.0	30.0	40.0
21	36.29364184591434	38.0	35.0	40.0	30.0	40.0
22	36.29567722093311	38.0	35.0	40.0	30.0	40.0
23	36.26081567296933	38.0	35.0	40.0	30.0	40.0
24	36.35379883789552	38.0	35.0	40.0	30.0	40.0
25	36.39976425820125	38.0	35.0	40.0	30.0	40.0
26	36.04990449760239	38.0	35.0	40.0	30.0	40.0
27	35.76840827222097	38.0	35.0	40.0	28.0	40.0
28	35.61366001569497	38.0	34.0	40.0	27.0	40.0
29	35.49832368438308	38.0	34.0	40.0	27.0	40.0
30	35.37436622578332	38.0	34.0	40.0	27.0	40.0
31	35.32337714668872	38.0	34.0	40.0	27.0	40.0
32	35.31553230688527	38.0	34.0	40.0	27.0	40.0
33	35.04926041166504	38.0	34.0	40.0	26.0	40.0
34	34.78351256337742	37.0	33.0	40.0	25.0	40.0
35	34.68052332775619	37.0	33.0	40.0	25.0	40.0
36	34.315118780446014	37.0	33.0	40.0	24.0	40.0
37	34.22968273743927	37.0	33.0	40.0	24.0	40.0
38	33.959190968836396	36.0	33.0	39.0	23.0	40.0
39	33.640747055078336	36.0	33.0	39.0	23.0	40.0
40	33.39006912554493	35.0	33.0	39.0	22.0	40.0
41	32.95467126234498	35.0	32.0	39.0	20.0	40.0
42	32.689171952965864	35.0	31.0	39.0	18.0	40.0
43	32.33964534026563	35.0	31.0	39.0	17.0	40.0
44	31.956160909377427	35.0	31.0	39.0	15.0	40.0
45	31.66514617260657	35.0	30.0	38.0	13.0	40.0
46	31.124689326364372	35.0	29.0	38.0	10.0	40.0
47	30.63925529282114	35.0	27.0	38.0	10.0	40.0
48	30.237910961729238	35.0	26.0	38.0	9.0	40.0
49	29.814271632932357	34.0	24.0	38.0	9.0	40.0
50	29.20513238134118	34.0	23.0	37.0	9.0	40.0
51	28.775634937590294	33.0	22.0	37.0	8.0	40.0
52	27.9737913076954	33.0	20.0	37.0	8.0	40.0
53	27.617684230787944	33.0	19.0	36.0	8.0	39.0
54	27.252070805033387	33.0	18.0	36.0	8.0	39.0
55	26.897821424265434	33.0	15.0	36.0	8.0	39.0
56	26.348780995974728	32.0	13.0	36.0	8.0	39.0
57	25.81928365798508	32.0	10.0	35.0	8.0	39.0
58	25.292533996949846	31.0	10.0	35.0	8.0	38.0
59	24.52522881443768	30.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	1.0
14	20.0
15	89.0
16	318.0
17	960.0
18	2169.0
19	4338.0
20	7659.0
21	12123.0
22	18198.0
23	25313.0
24	34274.0
25	44679.0
26	57133.0
27	69286.0
28	77827.0
29	81986.0
30	91445.0
31	106496.0
32	124133.0
33	143040.0
34	165945.0
35	180938.0
36	202374.0
37	225907.0
38	201784.0
39	12616.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.74021444148548	21.677457838282606	36.74013194772011	7.842195772511808
2	25.887019500257004	21.442773651914386	36.19715375357209	16.473053094256528
3	21.833191260737408	20.95970919890093	41.53021704321193	15.676882497149736
4	25.327331030558653	18.360097977210568	40.02142722674998	16.2911437654808
5	0.0	0.0	0.0	100.0
6	0.0	100.0	0.0	0.0
7	0.0	100.0	0.0	0.0
8	0.0	100.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	98.0640934252469	0.4957029209138617	1.3237605311752398	0.11644312266399866
12	98.71939005379016	0.8751213610202152	0.22738666096966134	0.178101924219958
13	94.29095551047777	3.6894278951610002	1.1585085973310094	0.8611079970302243
14	47.03434913476732	32.71327282380389	11.833889284905966	8.418488756522823
15	27.93334080712746	37.758982830720676	21.63383132774773	12.67384503440413
16	38.5059215716966	23.47598056531497	22.200394277894	15.817703585094433
17	27.634142265786448	23.17630609840449	21.82938385618164	27.360167779627425
18	19.905322540046495	36.26674464795257	25.77062925821183	18.057303553789108
19	25.581475284656367	32.211277109249245	29.50479415690314	12.702453449191244
20	20.599645065286413	34.56806052927154	28.431793520220488	16.400500885221557
21	26.37151172997887	34.430200755981325	18.321283602989237	20.87700391105057
22	25.904575865708612	34.16384107893384	18.84268650465455	21.088896550702994
23	19.218879227012266	36.96254783051973	26.869329875645935	16.949243066822063
24	22.51720206530545	39.626303242851066	17.025285396699825	20.831209295143655
25	22.106319656995154	40.050934612057205	20.328155968212403	17.514589762735238
26	21.339286280863774	36.41158466292836	21.95783087932008	20.291298176887786
27	24.526612700232462	39.700230347975626	17.028458233829635	18.744698717962276
28	21.38978727184657	39.31679298083818	14.711070874835805	24.582348872479447
29	22.55871335108712	43.18178453051529	15.004346786867838	19.255155331529753
30	23.6594763126556	42.51538297201769	18.50139499072474	15.323745724601967
31	18.931790347383362	45.35909113022804	14.8092701840034	20.899848338385198
32	20.404462701184315	51.60260003426664	14.646768042338337	13.346169222210705
33	22.422704399455963	45.41646660165876	14.599915814054823	17.560913184830454
34	20.721482011071085	47.74305518832904	15.18324192037025	16.35222088022963
35	21.117980891059577	49.01335341386699	16.03974930356225	13.828916391511182
36	20.275169588144585	46.21147382515129	21.18328845531482	12.3300681313893
37	20.707891692031737	47.71666775953279	16.494998551071046	15.08044199736443
38	16.522655114719214	51.96848103595247	20.06248373920971	11.4463801101186
39	17.42850011527975	49.414029862743064	20.68864314677756	12.468826875199625
40	18.579076619786235	48.018404570577644	15.96767302009675	17.43484578953937
41	19.823093177765603	50.77020621326119	13.610519435742646	15.796181173230561
42	18.176919513582916	50.98543033189992	13.692854559261194	17.144795595255975
43	18.517206295754956	49.24735015219042	15.477311041684736	16.75813251036989
44	19.39222189553751	49.12810435672842	16.380935056254405	15.098738691479664
45	21.58925296607391	44.101642895065815	20.075069326491285	14.234034812368987
46	23.63811254264822	44.17710353813645	17.36377423783164	14.821009681383696
47	22.39753322489281	43.5065772913701	16.666701920412553	17.42918756332454
48	22.487324515666412	43.1400617222583	17.616543595839776	16.756070166235514
49	21.662915668104315	45.79842331146896	15.384505555637814	17.15415546478891
50	19.0281388348919	47.248832924742416	16.24249359615706	17.48053464420862
51	18.517417818230275	46.71405122651308	19.499728193619212	15.268802761637437
52	22.99503133705472	44.05500218925762	17.6321962590135	15.317770214674159
53	22.10161328191927	45.587746926049626	16.308911653407733	16.00172813862337
54	18.510860621495336	45.73872109280971	19.001063958050864	16.749354327644085
55	20.40874603130956	44.600095608158846	17.72716985043246	17.26398851009914
56	20.737240435482473	41.729259692488625	20.406736567794013	17.126763304234892
57	22.733325154464286	40.092868942789515	18.585739577758833	18.588066324987363
58	21.604059539346355	41.211188269809604	19.159600053303663	18.025152137540374
59	22.19394284239672	38.725693423554716	19.33072173583804	19.74964199821052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	15.0
5	25.0
6	45.5
7	66.0
8	621.5
9	2210.0
10	3243.0
11	5577.0
12	7911.0
13	8721.0
14	8217.0
15	6903.0
16	7524.0
17	8145.0
18	9357.0
19	13117.5
20	15666.0
21	21368.0
22	27070.0
23	36997.0
24	46924.0
25	56019.5
26	70893.0
27	76671.0
28	80439.5
29	84208.0
30	83608.5
31	81474.5
32	79940.0
33	77991.0
34	76042.0
35	73227.5
36	69453.0
37	68493.0
38	66027.0
39	63561.0
40	65144.0
41	66727.0
42	71446.5
43	85855.5
44	95545.0
45	102227.0
46	108909.0
47	115496.0
48	116141.0
49	110199.0
50	104138.5
51	98078.0
52	90462.5
53	78878.5
54	74910.0
55	68985.0
56	63060.0
57	58866.5
58	48347.0
59	42021.0
60	34868.5
61	27716.0
62	26482.5
63	25249.0
64	20073.0
65	14233.0
66	13569.0
67	8877.0
68	4185.0
69	2603.0
70	1742.0
71	2463.0
72	1996.0
73	1529.0
74	926.0
75	209.0
76	95.0
77	60.0
78	25.0
79	18.5
80	12.0
81	10.0
82	10.0
83	12.0
84	16.5
85	21.0
86	16.5
87	10.5
88	9.0
89	5.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1891052.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.37545173181644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.20750983174916	41.37038566317825
2	5.092682959764851	4.723509455720379
3	1.658620652421099	2.3075784602324574
4	0.8668511686629126	1.6080245812398228
5	0.5273585252860542	1.2228244917382656
6	0.3493801346139675	0.9721596941307349
7	0.26501664009115283	0.8603186480472801
8	0.19882308171364532	0.7376408183345724
9	0.1563826030463578	0.6527082473344958
>10	1.3897157152067126	13.815016109969344
>50	0.15079694547114336	4.741352609294388
>100	0.09712395724607205	10.039312361781201
>500	0.027612357967338838	9.011685589651448
>1k	0.012125426759570531	7.937483269347389
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGGCAAAGTGGGCTGATGAGCCAGTCCTGCTTGAAGAACCAGTAGTCTTGGCACTAGC	3165	0.16736715859743675	No Hit
TACGCAAAGTGGGGGGGCTGGTGAGATGGCTCAGTGGTTTAGAGCACCCGACTGCTCTT	3088	0.16329535094751493	No Hit
TCGTCAAAGTAGGCATCAAAAAAAATGAAGAAGAACTCTAGATAATGTAGCCATATTCC	2547	0.13468693616040173	No Hit
TCATCAAAGTGGGCGGGTGCAGTGGCTCACACTTGTTATCCCAGCTCTTTGGGAGGCCG	2395	0.12664908209821835	No Hit
GCTCCAAAGTGGTGTTAACGCAGAGTTATTCAAAGTGGGGCTACTTGGGAGGCTGAGGC	2382	0.12596163405342634	No Hit
GGACCAAAGTGGGTGTATGACAGACACGTGTCTTTCCCCTAGGGAAGACATGACGTTAC	2271	0.12009188536327928	No Hit
GGTTCAAAGTGGGGAGGATAACTGATGCATCACCATCATCACCATGCTCCTGGTATGAA	2252	0.11908715360550634	No Hit
GGTGCAAAGTGGGGATGCAGAGATGACCTGTGACTTGTAGGCAGTTAAAATTACTTAGG	2233	0.11808242184773343	No Hit
TCAGCAAAGTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG	2137	0.11300588244003867	No Hit
GTTCCAAAGTGGGCGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCAACTGCTCTT	2036	0.10766493993819312	No Hit
CAGGCAAAGTGGGGGACTCGACTCGGTCCTCCGCCCGCCCCGGGAATACCTCTAGCTCT	2025	0.10708325313106146	No Hit
TCAGCAAAGTGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAA	1940	0.10258840053049836	No Hit
TGTACAAAGTGGGACGCGCGGCGACATGGAAGACATCCGCGGCGTGACGATCAACTCGT	1937	0.1024297586740079	No Hit
GATTCAAAGTGGGAGAGTGTGTCAAAGTGGGAAGCAGTATCAACGCAGAATCGCCCAAA	1937	0.1024297586740079	No Hit
CAGTCAAAGTGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGATTGCTCTTCT	1928	0.10195383310453651	No Hit
AAATCAAAGTGGGGAGTCATCCAAAGTGGGTCATGTAATTTAATGTGCCAAATAAGCCT	1907	0.1008433401091033	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.04854440808608119	0.0	0.0	0.0	0.0
12	0.10491514775902513	0.0	0.0	0.0	0.0
13	0.154041242652238	0.0	0.0	0.0	0.0
14	0.5326664734761392	0.0	0.0	0.0	0.0
15	0.9400058803248139	0.0	0.0	0.0	0.0
16	1.1127668620429263	0.0	0.0	0.0	0.0
17	1.2258256251017952	0.0	0.0	0.0	0.0
18	1.3851549296370487	0.0	0.0	0.0	0.0
19	1.5116453698787764	0.0	0.0	0.0	0.0
20	1.668965210898484	0.0	0.0	0.0	0.0
21	1.860498812301301	0.0	0.0	0.0	0.0
22	2.019299310648253	0.0	0.0	0.0	0.0
23	2.1666775953278914	0.0	0.0	0.0	0.0
24	2.3274875571903895	0.0	0.0	0.0	0.0
25	2.49950821024488	0.0	0.0	0.0	0.0
26	2.6977576502391263	0.0	0.0	0.0	0.0
27	2.899444330457333	0.0	0.0	0.0	0.0
28	3.0939921271334687	0.0	0.0	0.0	0.0
29	3.3694472706197396	0.0	0.0	0.0	0.0
30	3.6947159570440156	0.0	0.0	0.0	0.0
31	3.9599651410960672	0.0	0.0	0.0	0.0
32	4.173232676838078	0.0	0.0	0.0	0.0
33	4.333936877462915	0.0	0.0	0.0	0.0
34	4.50780835217646	0.0	0.0	0.0	0.0
35	4.671791151168767	0.0	0.0	0.0	0.0
36	4.8445521328868795	0.0	0.0	0.0	0.0
37	5.023605908245781	0.0	0.0	0.0	0.0
38	5.196472651201553	0.0	0.0	0.0	0.0
39	5.363205242373028	0.0	0.0	0.0	0.0
40	5.53929770307744	0.0	0.0	0.0	0.0
41	5.716500656777286	0.0	0.0	0.0	0.0
42	5.906712242709349	0.0	0.0	0.0	0.0
43	6.088568690866248	0.0	0.0	0.0	0.0
44	6.258738522261683	0.0	0.0	0.0	0.0
45	6.4267931289039115	0.0	0.0	0.0	0.0
46	6.582738073834035	0.0	0.0	0.0	0.0
47	6.7295875523253725	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTAAC	20	3.1387628E-4	53.000004	16
AGGTACT	45	7.6397555E-11	53.000004	39
GTTTCTT	20	3.1387628E-4	53.000004	33
TACTTAC	45	7.6397555E-11	53.000004	31
TAGACTT	20	3.1387628E-4	53.000004	36
TTTAACG	155	0.0	53.000004	49
CATGACT	90	0.0	53.000004	45
ATTACTC	20	3.1387628E-4	53.000004	44
TTCACGA	80	0.0	53.000004	20
ACATTAG	150	0.0	53.000004	35
CCTACAC	160	0.0	53.000004	32
TAAGTTA	20	3.1387628E-4	53.000004	37
ATTCGGC	20	3.1387628E-4	53.000004	28
TAAGTGT	90	0.0	53.000004	14
TAACGTT	160	0.0	53.000004	51
CTAAGCT	20	3.1387628E-4	53.000004	31
CACTGAA	20	3.1387628E-4	53.000004	46
CGACACC	20	3.1387628E-4	53.000004	16
TACATGT	20	3.1387628E-4	53.000004	31
TATTGGC	90	0.0	53.000004	25
>>END_MODULE