FastQCFastQC Report
Fri 17 Jun 2016
SRR1528158_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528158_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574408
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCGGTGGGGATTCTGCATGGGGCAAATCTTGAACATTCTTAGAAAGC35650.620638988314926No Hit
TATCGTCGGTGGGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTAT33880.5898246542527263No Hit
ACGCGTCGGTGGGCCTGCCAAAGAGGCTGCCTGGGCAGTGGGGAAAAGCACA32890.5725895182518349No Hit
GCAAGTCGGTGGGGTTGGACGCCTCGAACGTTGAGCAGGCTTTCCAAAACAT27060.47109371735769695No Hit
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA25890.45072492026573446No Hit
TAAGGTCGGTGGGGTTACAGTGAGCTATGATGGCAACACTGCACTCCAGCCT21110.3675088090695116No Hit
CTTTGTCGGTGGGAGAGGCCAAGGTGGGCAGATCACCTGAGGTCAGGACTTC20430.3556705338365761No Hit
TTGCGTCGGTGGGTGAGGCAAAGGTTGCAGTGAGCCGAGCCCGCACCACTGC18820.327641676299773No Hit
GTGGGTCGGTGGGTTGAAATAACCGCCGGGCGTGGTGGCTGATGCCTGTAAT18070.3145847550869765No Hit
GGATGTCGGTGGGAAAGAGGATGGTTCAGAAAATTTTGTCGGATAAGGTTAT14790.25748248631634657No Hit
TTAAGTCGGTGGGGAGTTCAAGACCAGCATGGTCAACATGGCAAAACCCTGT13770.23972507346694336No Hit
GTTCGTCGGTGGAGGGATTAAAGGCGTGAGCCATCGTGCCCTGCCTGAAACA13280.2311945516079163No Hit
GTAGGTCGGTGGGGGTACAAAGTGCAAAGTGGCTGGTCTGGTGTTGTGCTTA12840.22353449116307572No Hit
GAATGTCGGTGGGGTGTGTGCTTGAGCTTTCTTTAAACTGCTCCAAATAGTA12510.2177894458294453No Hit
GTGAGTCGGTGGGAGCCCAATACAGCCAAATACTTATAGTCAACTGATCTTC12060.20995529310176736No Hit
CTTAGTCGGTGGGCTGCGACACCGCATAAATCGCCGCAAGTTTTCAACCTGA11680.20333978635395047No Hit
GCTCGTCGGTGGGGGGAAATTAGGAAACCATAGCTACCTGAGTACTGAAATC11660.2029916017882759No Hit
TAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11210.19515744906059806No Hit
AAGGGTCGGTGGGGGAGCTGGGATTAACAGTCCCACCTTTTGGCTTGGTCCT11120.19359061851506246No Hit
AGAGGTCGGTGGGGTCATTTTAAATAGCAATGAAAGAAAGACAGGGGAGAAA11100.1932424339493879No Hit
GTTAGTCGGTGGGAGTCTTGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGTG10890.18958649600980487No Hit
TTGTGTCGGTGGGCAACTCAGGACAATTAGGTGATTGGCGTTTGGGTAGAAT10850.18889012687845572No Hit
CACTGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC10170.17705185164552026No Hit
CACGGTCGGTGGGGTAGCCTTGTATTATAGTTTGAAGTCAGGTAGCATGATG10150.17670366707984567No Hit
ATTTGTCGGTGGGTTCCTTTCCATTCCATTCCATTCCATTCCGTTCCATTCC9920.1726995445745881No Hit
GATGGTCGGTGGGTTGCTCAAAGCTCCACTCAGACCACTGTTGAATGTTTCC9720.16921769891784239No Hit
GGTGGTCGGTGGATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTAT9640.16782496065514407No Hit
TTGGGTCGGTGGGGATCCTCGGCGTGATTGCGATTATCCTCGGCGTGCTGTT9590.16695449924095765No Hit
AAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9250.1610353616244899No Hit
TGCCGTCGGTGGGGCTTTTCCATGCAAAGACTAGAAGTATTAAGGGTACTAT9160.15946853107895434No Hit
GGCCGTCGGTGGGATAGCTTGCAGGCTAACATCCTGGGTCCAACCCCACATT9110.15859806966476792No Hit
AAGCGTCGGTGGGAGAGTATTTGTCGGTGGGAGGGAACAGAGTTTGTGGCAA9100.1584239773819306No Hit
CGTAGTCGGTGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC8990.15650896227072045No Hit
GCTAGTCGGTGGGTACGCAGAGACACCAGGAAACAGAGGAACGACACCAGCC8770.15267893204830016No Hit
TCGTGTCGGTGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCC8770.15267893204830016No Hit
CAAGGTCGGTGGTAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCC8760.1525048397654629No Hit
AGGGGTCGGTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGG8550.14884890182587987No Hit
TTGAGTCGGTGGGTTGGACTATTTCAATATTTATAATTTCCCACTACATACT8430.14675979443183243No Hit
ACACGTCGGTGGGCAGATGCTTTCATTTTAGATTTAGTTTTATTACAGATAG8310.144670687037785No Hit
TTTTGTCGGTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA8170.14223339507806299No Hit
TTTTGTCGGTGGGTCAATGTCATTGAGCCTTCTCATTATTTCAACTATTATT7520.13091739669363936No Hit
ATTAGTCGGTGGGTACTACTCTTTTAAATAACATTTGTTTTTTTTCTAATTT7320.12743555103689364No Hit
TCTAGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC7220.1256946282085208No Hit
TCATGTCGGTGGGACTTTGGGAGGCCGAGGTGGTTCAATCGCCTGAGGTCAG6800.11838275232935475No Hit
GCTTGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6580.11455272210693444No Hit
GACTGTCGGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG6520.11350816840991072No Hit
GTTCGTCGGTGGGGGTATTTCTCTTCAATGCGTGCCAGCGTACCGTCTTCAT6400.1114190610158633No Hit
TAAAGTCGGTGGGGGTTCTGACAGGCAGAGCAGAGGGAGCAGTTCCCCCTTT6290.10950404590465312No Hit
GGGGGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6250.108807676773304No Hit
CTGAGTCGGTGGGCAGAGTAGGAGTCGGTGGGGGAATTCCTGGAGATTGTCT6170.1074149385106057No Hit
TCAGGTCGGTGGGACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGG6100.10619629253074471No Hit
GATCGTCGGTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC6030.10497764655088368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTA551.8189894E-1246.00000421
CGTTTGG900.046.00000439
CAGCCAC551.8189894E-1246.00000438
CTCTGGT351.0183794E-746.00000441
ACAGCAA351.0183794E-746.00000436
CTTCAGA351.0183794E-746.00000424
CTTCAAT900.046.00000423
TTGTTAG700.046.00000430
AAGTAGC453.092282E-1046.00000436
TCCTTCG453.092282E-1046.00000420
CAATGAA900.046.00000428
GTTTGAA1100.046.00000430
GTGTTGC351.0183794E-746.00000418
CCAATAC1100.046.00000417
GCCTTGT1100.046.00000417
AATCCAC750.046.00000446
TATACTG700.046.00000418
TAAACAG351.0183794E-746.00000433
GCCCAAT1100.046.00000415
TTGTATT1100.046.00000420
Produced by FastQC (version 0.11.5)