##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528158_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	574408
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.19333818470495	31.0	30.0	31.0	28.0	33.0
2	30.827296973579756	31.0	31.0	33.0	28.0	33.0
3	31.44884994637958	31.0	31.0	34.0	30.0	34.0
4	35.34961038147101	37.0	35.0	37.0	33.0	37.0
5	35.489362961518644	37.0	35.0	37.0	33.0	37.0
6	31.745036629016308	33.0	32.0	35.0	27.0	35.0
7	34.09913336861604	35.0	33.0	35.0	32.0	37.0
8	34.889799584963995	35.0	35.0	37.0	32.0	37.0
9	36.65337181933399	39.0	35.0	39.0	32.0	39.0
10	32.82881679920892	34.0	31.0	37.0	27.0	37.0
11	36.35978781632568	37.0	35.0	39.0	34.0	39.0
12	37.34774237127617	39.0	37.0	39.0	35.0	39.0
13	37.46577693903985	39.0	37.0	39.0	35.0	39.0
14	37.46538523140346	40.0	37.0	40.0	32.0	40.0
15	36.56362028384006	38.0	36.0	40.0	31.0	40.0
16	36.6704363448977	38.0	36.0	40.0	31.0	40.0
17	36.5184015542959	38.0	36.0	40.0	31.0	40.0
18	36.699882313616804	38.0	36.0	40.0	31.0	40.0
19	36.82276361053467	39.0	36.0	40.0	31.0	40.0
20	37.03911157226222	39.0	36.0	40.0	32.0	40.0
21	36.95636551022966	39.0	36.0	40.0	31.0	40.0
22	37.100783763457336	39.0	36.0	40.0	31.0	40.0
23	36.88970383420844	39.0	35.0	40.0	31.0	40.0
24	36.97052965836131	39.0	36.0	40.0	31.0	40.0
25	36.920955139900556	39.0	35.0	40.0	31.0	40.0
26	36.72955286138076	39.0	35.0	40.0	31.0	40.0
27	36.22578724530299	38.0	35.0	40.0	30.0	40.0
28	35.958992562777674	38.0	35.0	40.0	30.0	40.0
29	35.8862655116224	38.0	35.0	40.0	30.0	40.0
30	35.878138883859556	38.0	35.0	40.0	30.0	40.0
31	35.76831102630882	38.0	35.0	40.0	30.0	40.0
32	35.59938406150332	38.0	35.0	40.0	30.0	40.0
33	35.43059462960126	38.0	35.0	40.0	29.0	40.0
34	35.191771354159414	37.0	34.0	40.0	27.0	40.0
35	35.093745212462224	37.0	34.0	40.0	27.0	40.0
36	34.830054595339895	37.0	34.0	40.0	26.0	40.0
37	34.44497117031796	36.0	33.0	40.0	25.0	40.0
38	33.93866728875643	35.0	33.0	39.0	24.0	40.0
39	33.41052004846729	35.0	33.0	39.0	22.0	40.0
40	32.890812802050114	35.0	33.0	39.0	20.0	40.0
41	32.30101774348547	35.0	32.0	38.0	17.0	40.0
42	31.669733012075042	35.0	31.0	38.0	14.0	40.0
43	31.179795546023037	35.0	31.0	38.0	10.0	40.0
44	30.668133103995768	35.0	29.0	37.0	10.0	40.0
45	30.306658333449395	35.0	27.0	37.0	9.0	40.0
46	29.871603459561843	35.0	26.0	36.0	9.0	40.0
47	29.34826290720185	34.0	24.0	36.0	8.0	39.0
48	28.936858469937743	34.0	23.0	35.0	8.0	39.0
49	28.612413127950862	34.0	23.0	35.0	8.0	39.0
50	28.178784069859752	34.0	20.0	35.0	8.0	39.0
51	27.704252029916017	33.0	20.0	35.0	8.0	38.0
52	26.727503795211767	33.0	15.0	35.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	5.0
16	29.0
17	76.0
18	227.0
19	462.0
20	958.0
21	1682.0
22	2866.0
23	4282.0
24	5995.0
25	8661.0
26	11674.0
27	15219.0
28	19907.0
29	25128.0
30	28613.0
31	29783.0
32	30802.0
33	36825.0
34	48180.0
35	64797.0
36	89161.0
37	86907.0
38	59268.0
39	2900.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.941045389339976	22.374166097965208	38.50120471859723	6.183583794097576
2	18.301103048704057	26.589288450021588	34.130966142532834	20.978642358741524
3	20.862870990654724	29.826708541663766	33.957570228826896	15.352850238854613
4	22.575939053773624	30.29484269021323	27.490390105987384	19.638828150025766
5	100.0	0.0	0.0	0.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	0.0	100.0
8	100.0	0.0	0.0	0.0
9	100.0	0.0	0.0	0.0
10	0.0	0.0	100.0	0.0
11	99.3743123354828	0.3713388392919319	0.110026322753165	0.1443225024721104
12	97.95197838470216	1.037590005710227	0.25469700979095	0.7557345997966601
13	93.82337989721591	3.3728638876895864	1.303951198451275	1.4998050166432222
14	41.39653347446414	32.718903636439606	13.99928273979471	11.885280149301542
15	26.41171432152756	41.57497806437237	23.214161362655116	8.799146251444965
16	31.605931672260834	32.35104664280441	23.39173549114915	12.651286193785602
17	21.548620492750796	34.91037729279537	22.294431832425733	21.246570382028104
18	19.354535452152476	38.803080737037085	23.310956671912646	18.531427138897786
19	17.916881380482167	38.27401428949457	29.346562025598526	14.46254230442473
20	21.244655366916895	34.784334480021165	28.35266918288046	15.618340970181475
21	25.131265581259317	34.89679809473405	18.751653876686955	21.220282447319676
22	29.591161682984918	37.0508419102798	17.540145680422278	15.817850726313004
23	23.270219077728722	36.680025347836384	23.50768095151878	16.542074622916115
24	25.579379117282492	36.02195651871144	17.626321360426736	20.772343003579337
25	27.788610186487656	34.1616063843122	20.57126641690227	17.47851701229788
26	26.63524881269063	37.73189092073926	16.2684015542959	19.364458712274203
27	24.668702385760643	41.789633849110736	15.791910976170248	17.74975278895837
28	24.789348337766885	43.1957772175875	15.425968997646272	16.588905446999345
29	24.904771521288005	45.43216668291528	15.124441163772092	14.538620632024625
30	27.24787955599504	49.941156808401	12.504526399353768	10.306437236250192
31	21.432152755532652	50.981706382919455	13.592254982521135	13.993885879026754
32	20.78452946337795	55.26507290984806	15.69633431289258	8.254063313881423
33	20.76886115792259	55.4711981727274	14.096078049052242	9.663862620297767
34	17.291367808247795	56.068160610576456	15.351631592874751	11.288839988300998
35	18.657992228520495	56.06833470285929	14.046461748443614	11.227211320176599
36	17.26873581147895	56.30074790044707	14.280789961142604	12.14972632693138
37	17.44195763290205	57.56291695101739	13.104622498293898	11.89050291778666
38	16.34865809668389	59.12852885057311	13.626899346805754	10.895913705937243
39	17.064873748276487	55.4067840280776	15.385405495745186	12.142936727900725
40	20.520083285748107	54.71650812662776	13.84085876241278	10.922549825211348
41	20.673458586927758	53.406115513711505	15.09362682970989	10.826799069650841
42	19.118640408907954	52.42127546970098	14.66657845991003	13.793505661481037
43	20.89664489352516	52.4237127616607	13.853219314494227	12.826423030319912
44	20.568306848094036	50.89257113410677	13.985355357167728	14.553766660631467
45	20.09529811562513	52.17615353546607	15.556712302057074	12.171836046851716
46	19.37177058815337	53.892877536524566	13.94984053146892	12.785511343853152
47	22.464345900474925	52.04349521594407	13.09330649990947	12.398852383671537
48	21.490995947131655	54.49262545089901	12.856366902967926	11.160011699001407
49	19.255825127783734	53.4602582136739	13.197761869611844	14.086154788930516
50	18.464923886853942	53.66342390774502	15.841875461344548	12.02977674405649
51	18.55423322794947	53.810705979025364	14.463935042687428	13.171125750337739
52	19.72587429144441	50.0985362320859	15.666042255678892	14.509547220790797
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	3.0
8	6.0
9	13.5
10	21.0
11	87.0
12	153.0
13	1641.5
14	4153.5
15	5177.0
16	5147.5
17	5118.0
18	5397.5
19	5677.0
20	5411.5
21	5146.0
22	5362.0
23	5578.0
24	8140.5
25	10703.0
26	13305.5
27	15908.0
28	20334.5
29	24761.0
30	29405.5
31	34050.0
32	34157.0
33	34264.0
34	32538.5
35	30813.0
36	28882.0
37	26951.0
38	25107.0
39	22535.5
40	21808.0
41	23671.5
42	25535.0
43	23589.0
44	21643.0
45	23795.0
46	25947.0
47	28585.0
48	31223.0
49	30789.0
50	30355.0
51	29836.0
52	29317.0
53	31509.5
54	33702.0
55	32856.0
56	32010.0
57	29955.0
58	27900.0
59	27566.5
60	27233.0
61	20261.0
62	13289.0
63	11494.0
64	9499.0
65	9299.0
66	5747.5
67	2196.0
68	1744.0
69	1292.0
70	949.0
71	606.0
72	436.0
73	266.0
74	220.5
75	175.0
76	117.5
77	60.0
78	48.5
79	37.0
80	45.0
81	53.0
82	33.5
83	14.0
84	9.0
85	4.0
86	3.0
87	2.0
88	2.0
89	12.0
90	22.0
91	11.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	574408.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.49461491451419
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.48497730188905	35.88660829032386
2	5.760350768092951	4.780470737890861
3	1.8087761254813255	2.251634063802438
4	0.8852636437536197	1.4693469598150448
5	0.6017430740006284	1.2484548566566045
6	0.4165294505225278	1.037023748999189
7	0.35833466468128145	1.0408271245029939
8	0.29665781173876266	0.9847761327585922
9	0.25317007809192704	0.9454675408471799
>10	2.8068293468380503	23.715777048631363
>50	0.19264332092858083	5.461986311162568
>100	0.10702406718141107	8.798599381765507
>500	0.017627493418114766	5.205980193165676
>1k	0.010072853381779866	7.173047609678114
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGGTCGGTGGGGATTCTGCATGGGGCAAATCTTGAACATTCTTAGAAAGC	3565	0.620638988314926	No Hit
TATCGTCGGTGGGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTAT	3388	0.5898246542527263	No Hit
ACGCGTCGGTGGGCCTGCCAAAGAGGCTGCCTGGGCAGTGGGGAAAAGCACA	3289	0.5725895182518349	No Hit
GCAAGTCGGTGGGGTTGGACGCCTCGAACGTTGAGCAGGCTTTCCAAAACAT	2706	0.47109371735769695	No Hit
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA	2589	0.45072492026573446	No Hit
TAAGGTCGGTGGGGTTACAGTGAGCTATGATGGCAACACTGCACTCCAGCCT	2111	0.3675088090695116	No Hit
CTTTGTCGGTGGGAGAGGCCAAGGTGGGCAGATCACCTGAGGTCAGGACTTC	2043	0.3556705338365761	No Hit
TTGCGTCGGTGGGTGAGGCAAAGGTTGCAGTGAGCCGAGCCCGCACCACTGC	1882	0.327641676299773	No Hit
GTGGGTCGGTGGGTTGAAATAACCGCCGGGCGTGGTGGCTGATGCCTGTAAT	1807	0.3145847550869765	No Hit
GGATGTCGGTGGGAAAGAGGATGGTTCAGAAAATTTTGTCGGATAAGGTTAT	1479	0.25748248631634657	No Hit
TTAAGTCGGTGGGGAGTTCAAGACCAGCATGGTCAACATGGCAAAACCCTGT	1377	0.23972507346694336	No Hit
GTTCGTCGGTGGAGGGATTAAAGGCGTGAGCCATCGTGCCCTGCCTGAAACA	1328	0.2311945516079163	No Hit
GTAGGTCGGTGGGGGTACAAAGTGCAAAGTGGCTGGTCTGGTGTTGTGCTTA	1284	0.22353449116307572	No Hit
GAATGTCGGTGGGGTGTGTGCTTGAGCTTTCTTTAAACTGCTCCAAATAGTA	1251	0.2177894458294453	No Hit
GTGAGTCGGTGGGAGCCCAATACAGCCAAATACTTATAGTCAACTGATCTTC	1206	0.20995529310176736	No Hit
CTTAGTCGGTGGGCTGCGACACCGCATAAATCGCCGCAAGTTTTCAACCTGA	1168	0.20333978635395047	No Hit
GCTCGTCGGTGGGGGGAAATTAGGAAACCATAGCTACCTGAGTACTGAAATC	1166	0.2029916017882759	No Hit
TAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1121	0.19515744906059806	No Hit
AAGGGTCGGTGGGGGAGCTGGGATTAACAGTCCCACCTTTTGGCTTGGTCCT	1112	0.19359061851506246	No Hit
AGAGGTCGGTGGGGTCATTTTAAATAGCAATGAAAGAAAGACAGGGGAGAAA	1110	0.1932424339493879	No Hit
GTTAGTCGGTGGGAGTCTTGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGTG	1089	0.18958649600980487	No Hit
TTGTGTCGGTGGGCAACTCAGGACAATTAGGTGATTGGCGTTTGGGTAGAAT	1085	0.18889012687845572	No Hit
CACTGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	1017	0.17705185164552026	No Hit
CACGGTCGGTGGGGTAGCCTTGTATTATAGTTTGAAGTCAGGTAGCATGATG	1015	0.17670366707984567	No Hit
ATTTGTCGGTGGGTTCCTTTCCATTCCATTCCATTCCATTCCGTTCCATTCC	992	0.1726995445745881	No Hit
GATGGTCGGTGGGTTGCTCAAAGCTCCACTCAGACCACTGTTGAATGTTTCC	972	0.16921769891784239	No Hit
GGTGGTCGGTGGATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTAT	964	0.16782496065514407	No Hit
TTGGGTCGGTGGGGATCCTCGGCGTGATTGCGATTATCCTCGGCGTGCTGTT	959	0.16695449924095765	No Hit
AAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	925	0.1610353616244899	No Hit
TGCCGTCGGTGGGGCTTTTCCATGCAAAGACTAGAAGTATTAAGGGTACTAT	916	0.15946853107895434	No Hit
GGCCGTCGGTGGGATAGCTTGCAGGCTAACATCCTGGGTCCAACCCCACATT	911	0.15859806966476792	No Hit
AAGCGTCGGTGGGAGAGTATTTGTCGGTGGGAGGGAACAGAGTTTGTGGCAA	910	0.1584239773819306	No Hit
CGTAGTCGGTGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC	899	0.15650896227072045	No Hit
GCTAGTCGGTGGGTACGCAGAGACACCAGGAAACAGAGGAACGACACCAGCC	877	0.15267893204830016	No Hit
TCGTGTCGGTGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCC	877	0.15267893204830016	No Hit
CAAGGTCGGTGGTAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCC	876	0.1525048397654629	No Hit
AGGGGTCGGTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGG	855	0.14884890182587987	No Hit
TTGAGTCGGTGGGTTGGACTATTTCAATATTTATAATTTCCCACTACATACT	843	0.14675979443183243	No Hit
ACACGTCGGTGGGCAGATGCTTTCATTTTAGATTTAGTTTTATTACAGATAG	831	0.144670687037785	No Hit
TTTTGTCGGTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA	817	0.14223339507806299	No Hit
TTTTGTCGGTGGGTCAATGTCATTGAGCCTTCTCATTATTTCAACTATTATT	752	0.13091739669363936	No Hit
ATTAGTCGGTGGGTACTACTCTTTTAAATAACATTTGTTTTTTTTCTAATTT	732	0.12743555103689364	No Hit
TCTAGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC	722	0.1256946282085208	No Hit
TCATGTCGGTGGGACTTTGGGAGGCCGAGGTGGTTCAATCGCCTGAGGTCAG	680	0.11838275232935475	No Hit
GCTTGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	658	0.11455272210693444	No Hit
GACTGTCGGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG	652	0.11350816840991072	No Hit
GTTCGTCGGTGGGGGTATTTCTCTTCAATGCGTGCCAGCGTACCGTCTTCAT	640	0.1114190610158633	No Hit
TAAAGTCGGTGGGGGTTCTGACAGGCAGAGCAGAGGGAGCAGTTCCCCCTTT	629	0.10950404590465312	No Hit
GGGGGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	625	0.108807676773304	No Hit
CTGAGTCGGTGGGCAGAGTAGGAGTCGGTGGGGGAATTCCTGGAGATTGTCT	617	0.1074149385106057	No Hit
TCAGGTCGGTGGGACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGG	610	0.10619629253074471	No Hit
GATCGTCGGTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC	603	0.10497764655088368	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02228381220317266	0.0	0.0	0.0	0.0
12	0.05675408420495536	0.0	0.0	0.0	0.0
13	0.09522847871199565	0.0	0.0	0.0	0.0
14	0.4059832035765519	0.0	0.0	0.0	0.0
15	0.7952535480007242	0.0	0.0	0.0	0.0
16	1.147790420746229	0.0	0.0	0.0	0.0
17	1.324494087826075	0.0	0.0	0.0	0.0
18	1.482047603793819	0.0	0.0	0.0	0.0
19	1.6286333059428142	0.0	0.0	0.0	0.0
20	1.7912354981128396	0.0	0.0	0.0	0.0
21	1.9867411317391124	0.0	0.0	0.0	0.0
22	2.201919193325998	0.0	0.0	0.0	0.0
23	2.3761855684461217	0.0	0.0	0.0	0.0
24	2.5741284940321165	0.0	0.0	0.0	0.0
25	2.8183799668528295	0.0	0.0	0.0	0.0
26	3.1134663862620298	0.0	0.0	0.0	0.0
27	3.3777384716090304	0.0	0.0	0.0	0.0
28	3.6390509881477975	0.0	0.0	0.0	0.0
29	4.0093452737427056	0.0	0.0	0.0	0.0
30	4.489491789807942	0.0	0.0	0.0	0.0
31	4.8794585033634625	0.0	0.0	0.0	0.0
32	5.138333727942507	0.0	0.0	0.0	0.0
33	5.3851965850057795	0.0	0.0	0.0	0.0
34	5.62126572053314	0.0	0.0	0.0	0.0
35	5.845496580827565	0.0	0.0	0.0	0.0
36	6.068682887424966	0.0	0.0	0.0	0.0
37	6.3005738081642315	0.0	0.0	0.0	0.0
38	6.533857467166196	0.0	0.0	0.0	0.0
39	6.787858107825796	0.0	0.0	0.0	0.0
40	7.048822439798888	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTTGTA	55	1.8189894E-12	46.000004	21
CGTTTGG	90	0.0	46.000004	39
CAGCCAC	55	1.8189894E-12	46.000004	38
CTCTGGT	35	1.0183794E-7	46.000004	41
ACAGCAA	35	1.0183794E-7	46.000004	36
CTTCAGA	35	1.0183794E-7	46.000004	24
CTTCAAT	90	0.0	46.000004	23
TTGTTAG	70	0.0	46.000004	30
AAGTAGC	45	3.092282E-10	46.000004	36
TCCTTCG	45	3.092282E-10	46.000004	20
CAATGAA	90	0.0	46.000004	28
GTTTGAA	110	0.0	46.000004	30
GTGTTGC	35	1.0183794E-7	46.000004	18
CCAATAC	110	0.0	46.000004	17
GCCTTGT	110	0.0	46.000004	17
AATCCAC	75	0.0	46.000004	46
TATACTG	70	0.0	46.000004	18
TAAACAG	35	1.0183794E-7	46.000004	33
GCCCAAT	110	0.0	46.000004	15
TTGTATT	110	0.0	46.000004	20
>>END_MODULE