FastQCFastQC Report
Fri 17 Jun 2016
SRR1528157_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528157_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences573998
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGTCGGTGGGGATTCTGCATGGGGCAAATCTTGAACATTCTTAGAAAGC34610.6029637733929387No Hit
TATCGTCGGTGGGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTAT33880.5902459590451534No Hit
ACGCGTCGGTGGGCCTGCCAAAGAGGCTGCCTGGGCAGTGGGGAAAAGCACA32570.5674235798731007No Hit
GCAAGTCGGTGGGGTTGGACGCCTCGAACGTTGAGCAGGCTTTCCAAAACAT26280.45784131652026666No Hit
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA24130.4203847400165157No Hit
TAAGGTCGGTGGGGTTACAGTGAGCTATGATGGCAACACTGCACTCCAGCCT20650.35975735107090967No Hit
CTTTGTCGGTGGGAGAGGCCAAGGTGGGCAGATCACCTGAGGTCAGGACTTC20350.3545308520238747No Hit
TTGCGTCGGTGGGTGAGGCAAAGGTTGCAGTGAGCCGAGCCCGCACCACTGC18640.3247398074557751No Hit
GTGGGTCGGTGGGTTGAAATAACCGCCGGGCGTGGTGGCTGATGCCTGTAAT16840.29338081317356507No Hit
GGATGTCGGTGGGAAAGAGGATGGTTCAGAAAATTTTGTCGGATAAGGTTAT14230.2479102714643605No Hit
TTAAGTCGGTGGGGAGTTCAAGACCAGCATGGTCAACATGGCAAAACCCTGT13530.23571510702127882No Hit
GAATGTCGGTGGGGTGTGTGCTTGAGCTTTCTTTAAACTGCTCCAAATAGTA12940.22543632556211No Hit
GTTCGTCGGTGGAGGGATTAAAGGCGTGAGCCATCGTGCCCTGCCTGAAACA12440.2167254938170516No Hit
CTTAGTCGGTGGGCTGCGACACCGCATAAATCGCCGCAAGTTTTCAACCTGA12370.21550597737274346No Hit
GTAGGTCGGTGGGGGTACAAAGTGCAAAGTGGCTGGTCTGGTGTTGTGCTTA11920.20766622880219096No Hit
GTGAGTCGGTGGGAGCCCAATACAGCCAAATACTTATAGTCAACTGATCTTC11630.2026139463900571No Hit
AGAGGTCGGTGGGGTCATTTTAAATAGCAATGAAAGAAAGACAGGGGAGAAA11100.19338046474029524No Hit
AAGGGTCGGTGGGGGAGCTGGGATTAACAGTCCCACCTTTTGGCTTGGTCCT10970.19111564848658008No Hit
TAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10920.19024456531207426No Hit
GTTAGTCGGTGGGAGTCTTGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGTG10810.18832818232816143No Hit
GCTCGTCGGTGGGGGGAAATTAGGAAACCATAGCTACCTGAGTACTGAAATC10490.1827532500113241No Hit
TTGTGTCGGTGGGCAACTCAGGACAATTAGGTGATTGGCGTTTGGGTAGAAT10100.17595880125017857No Hit
CACTGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC10100.17595880125017857No Hit
ATTTGTCGGTGGGTTCCTTTCCATTCCATTCCATTCCATTCCGTTCCATTCC9840.17142916874274822No Hit
GGTGGTCGGTGGATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTAT9830.17125495210784705No Hit
TTGGGTCGGTGGGGATCCTCGGCGTGATTGCGATTATCCTCGGCGTGCTGTT9780.17038386893334123No Hit
CACGGTCGGTGGGGTAGCCTTGTATTATAGTTTGAAGTCAGGTAGCATGATG9570.16672531960041673No Hit
GATGGTCGGTGGGTTGCTCAAAGCTCCACTCAGACCACTGTTGAATGTTTCC9360.16306677026749222No Hit
AAGCGTCGGTGGGAGAGTATTTGTCGGTGGGAGGGAACAGAGTTTGTGGCAA9250.1611503872835794No Hit
TGCCGTCGGTGGGGCTTTTCCATGCAAAGACTAGAAGTATTAAGGGTACTAT9120.1588855710298642No Hit
AAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9070.15801448785535838No Hit
GGCCGTCGGTGGGATAGCTTGCAGGCTAACATCCTGGGTCCAACCCCACATT8960.15609810487144554No Hit
AGGGGTCGGTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGG8790.15313642207812572No Hit
CGTAGTCGGTGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC8580.1494778727452012No Hit
TTGAGTCGGTGGGTTGGACTATTTCAATATTTATAATTTCCCACTACATACT8420.14669040658678253No Hit
CAAGGTCGGTGGTAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCC8390.14616775668207904No Hit
TCGTGTCGGTGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCC8390.14616775668207904No Hit
ACACGTCGGTGGGCAGATGCTTTCATTTTAGATTTAGTTTTATTACAGATAG8270.14407715706326502No Hit
GCTAGTCGGTGGGTACGCAGAGACACCAGGAAACAGAGGAACGACACCAGCC8000.13937330792093353No Hit
ATTAGTCGGTGGGTACTACTCTTTTAAATAACATTTGTTTTTTTTCTAATTT7910.13780535820682302No Hit
TTTTGTCGGTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA7380.12857187655706118No Hit
TCTAGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC7320.12752657674765416No Hit
GTTCGTCGGTGGGGGTATTTCTCTTCAATGCGTGCCAGCGTACCGTCTTCAT7100.1236938107798285No Hit
TTTTGTCGGTGGGTCAATGTCATTGAGCCTTCTCATTATTTCAACTATTATT6960.12125477789121217No Hit
TCATGTCGGTGGGACTTTGGGAGGCCGAGGTGGTTCAATCGCCTGAGGTCAG6900.12020947808180515No Hit
TAAAGTCGGTGGGGGTTCTGACAGGCAGAGCAGAGGGAGCAGTTCCCCCTTT6670.11620249547907834No Hit
TCAGGTCGGTGGGACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGG6450.11236972951125265No Hit
GATCGTCGGTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC6180.10766588036892114No Hit
GGGGGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6010.10470419757560132No Hit
CTGAGTCGGTGGGCAGAGTAGGAGTCGGTGGGGGAATTCCTGGAGATTGTCT5930.10331046449639197No Hit
GCTTGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5920.1031362478614908No Hit
TTAAGTCGGTGGGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTC5920.1031362478614908No Hit
AAAGGTCGGTGGGAATCACGTCAGGGGTGGGAAATCACAGCCACATGCAGTT5750.10017456506817098No Hit
GACTGTCGGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5740.1000003484332698No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCATG351.0183794E-746.00000417
CGTTTGG750.046.00000439
CGTCATA1650.046.00000442
ATCCTAT351.0183794E-746.00000415
CATTGTA1400.046.00000427
TCCTTAA351.0183794E-746.00000429
TTCACAC1650.046.00000419
CCTATTT351.0183794E-746.00000417
ACTCGGG351.0183794E-746.00000423
TAAACTG1500.046.00000434
CAGCATG1400.046.00000425
TAGGAGG351.0183794E-746.00000436
TCGATTA351.0183794E-746.00000421
AGCTCCA750.046.00000422
CACACCG1650.046.00000421
CCAGCTA351.0183794E-746.00000417
CTCCCGA700.046.00000435
AATTCAC1650.046.00000417
CTGGGCA3250.046.00000431
GGTCCCA351.0183794E-746.00000413
Produced by FastQC (version 0.11.5)