##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528157_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 573998 Sequences flagged as poor quality 0 Sequence length 52 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.22349381008296 31.0 30.0 31.0 28.0 33.0 2 30.84641235683748 31.0 31.0 33.0 28.0 33.0 3 31.461073035097684 31.0 31.0 34.0 30.0 34.0 4 35.3805762389416 37.0 35.0 37.0 33.0 37.0 5 35.674436147861144 37.0 35.0 37.0 35.0 37.0 6 31.867276541033245 33.0 32.0 35.0 28.0 35.0 7 34.10742197708006 35.0 35.0 35.0 32.0 37.0 8 34.88873654612037 35.0 35.0 37.0 32.0 37.0 9 36.64412071122199 39.0 35.0 39.0 32.0 39.0 10 32.801852968128806 34.0 31.0 37.0 27.0 37.0 11 36.34815626535284 37.0 35.0 39.0 34.0 39.0 12 37.3601197216715 39.0 37.0 39.0 35.0 39.0 13 37.46221241188994 39.0 37.0 39.0 35.0 39.0 14 37.41877323614368 40.0 37.0 40.0 32.0 40.0 15 36.561742375408976 38.0 36.0 40.0 31.0 40.0 16 36.65381412478789 38.0 36.0 40.0 31.0 40.0 17 36.53346701556451 38.0 36.0 40.0 31.0 40.0 18 36.609352297394764 38.0 36.0 40.0 31.0 40.0 19 36.79392611124081 39.0 36.0 40.0 31.0 40.0 20 37.03629280938261 39.0 36.0 40.0 32.0 40.0 21 37.000412893424716 39.0 36.0 40.0 31.0 40.0 22 37.069350415855105 39.0 36.0 40.0 31.0 40.0 23 36.985283920849895 39.0 36.0 40.0 31.0 40.0 24 37.114496914623395 39.0 36.0 40.0 31.0 40.0 25 37.003331022059314 39.0 36.0 40.0 31.0 40.0 26 36.657425635629394 39.0 35.0 40.0 31.0 40.0 27 36.11422513667295 38.0 35.0 40.0 30.0 40.0 28 35.899884668587696 38.0 35.0 40.0 30.0 40.0 29 35.84153777539294 38.0 35.0 40.0 30.0 40.0 30 35.689802055059424 38.0 35.0 40.0 30.0 40.0 31 35.64360851431538 38.0 35.0 40.0 30.0 40.0 32 35.56271798856442 38.0 35.0 40.0 30.0 40.0 33 35.48511841504674 38.0 35.0 40.0 29.0 40.0 34 35.314898309750205 37.0 34.0 40.0 28.0 40.0 35 35.02292690915299 37.0 34.0 40.0 27.0 40.0 36 34.802924400433454 37.0 34.0 40.0 26.0 40.0 37 34.42370879341043 36.0 33.0 40.0 25.0 40.0 38 33.96416015386813 35.0 33.0 39.0 24.0 40.0 39 33.435569461914504 35.0 33.0 39.0 22.0 40.0 40 32.849980313520255 35.0 33.0 39.0 20.0 40.0 41 32.229079195397894 35.0 32.0 38.0 17.0 40.0 42 31.60659270589793 35.0 31.0 38.0 14.0 40.0 43 31.164702664469214 35.0 31.0 38.0 10.0 40.0 44 30.57774068899195 35.0 29.0 37.0 10.0 40.0 45 30.205018135951693 35.0 27.0 37.0 9.0 40.0 46 29.747685532005338 35.0 25.0 36.0 9.0 40.0 47 29.33683915274966 34.0 24.0 36.0 8.0 39.0 48 28.90435158310656 34.0 23.0 35.0 8.0 39.0 49 28.41446660092892 34.0 22.0 35.0 8.0 39.0 50 27.967660166063297 33.0 20.0 35.0 8.0 39.0 51 27.50003484332698 33.0 20.0 35.0 8.0 38.0 52 26.357111000386762 33.0 15.0 35.0 8.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 0.0 12 0.0 13 1.0 14 3.0 15 7.0 16 20.0 17 60.0 18 217.0 19 512.0 20 964.0 21 1763.0 22 2852.0 23 4337.0 24 6142.0 25 8622.0 26 11689.0 27 15558.0 28 20062.0 29 25388.0 30 28721.0 31 29686.0 32 31316.0 33 37177.0 34 48284.0 35 64309.0 36 88446.0 37 87041.0 38 58328.0 39 2492.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.893668619054424 22.412970080035123 38.531667357725986 6.161693943184471 2 18.30703242868442 26.66925668730553 34.17137341941958 20.85233746459047 3 20.80425367335775 29.891567566437516 34.001686417025844 15.302492343178898 4 22.62655967442395 30.20184739319649 27.47814452315165 19.693448409227905 5 100.0 0.0 0.0 0.0 6 0.0 0.0 100.0 0.0 7 0.0 0.0 0.0 100.0 8 100.0 0.0 0.0 0.0 9 100.0 0.0 0.0 0.0 10 0.0 0.0 100.0 0.0 11 99.35470158432608 0.3874577960201952 0.11933839490729933 0.13850222474642768 12 98.00818121317496 1.028923445726292 0.2414642559730173 0.7214310851257322 13 93.8642643354158 3.380325366987341 1.2993076630929028 1.456102634503953 14 41.263906842880985 32.89332018578462 14.038724873605831 11.804048097728563 15 26.188941424883012 41.790912163457016 23.159662577221525 8.860483834438448 16 31.602200704532073 32.39175049390416 23.320987181140005 12.685061620423765 17 21.475858800901744 34.888449088672786 22.256697758528777 21.378994351896697 18 19.479684598204177 38.864421130387214 23.290673486667202 18.36522078474141 19 17.977588772086314 38.301352966386645 29.23825518555814 14.482803075968906 20 21.153035376429884 34.836880964742036 28.35671901295823 15.653364645869846 21 25.21158610308747 34.93705552981021 18.57863616249534 21.272722204606982 22 29.58651423872557 36.9950766378977 17.765392910776693 15.653016212600043 23 23.076387025738764 36.717897971769936 23.66558768497451 16.540127317516788 24 25.531099411496207 36.10953348269506 17.49030484426775 20.86906226154098 25 27.731978160202647 34.261791852933285 20.556169185258486 17.45006080160558 26 26.441207112219907 37.76302356454204 16.362077916647795 19.433691406590267 27 24.65496395457824 41.86634796636922 15.722006000020905 17.756682079031634 28 24.67430200105227 43.37210234182001 15.358415882982172 16.595179774145556 29 24.783013181230594 45.69893971755999 15.103711162756664 14.414335938452746 30 27.094345276464377 50.01829274666463 12.581054289387769 10.306307687483232 31 21.30808818149192 51.0454740260419 13.58436788978359 14.06206990268259 32 20.720107038700483 55.38329401844606 15.629845400158187 8.266753542695271 33 20.651988334454128 55.71291189167906 13.959107871455997 9.67599190241081 34 17.31399760974777 56.23869769581079 15.161725302178752 11.28557939226269 35 18.437346471590494 56.22232133213008 14.185763713462416 11.154568482817014 36 17.17845706779466 56.467966787340714 14.163986634099771 12.189589510764847 37 17.286471381433387 57.82546280649061 13.039244039177836 11.848821772898162 38 16.2288021909484 59.354562211018155 13.57548284140363 10.841152756629814 39 16.87619120624114 55.67615218171492 15.34186530266656 12.105791309377384 40 20.398154697403125 54.88712504224753 13.740117561385231 10.974602698964107 41 20.611570075157058 53.51534325903574 15.024616810511535 10.848469855295663 42 19.031773629873275 52.634678169610346 14.592907989226445 13.740640211289934 43 20.810177038944385 52.53032240530455 13.757887658145151 12.901612897605915 44 20.447283788445255 51.058714490294385 13.98332398370726 14.510677737553094 45 20.02864121477775 52.44948588671041 15.44953118303548 12.07234171547636 46 19.232819626549222 54.28119958606128 13.85632005686431 12.629660730525194 47 22.47412011888543 52.40070522893808 12.789243168094663 12.335931484081826 48 21.46470893626807 54.72998163756668 12.673040672615585 11.132268753549663 49 19.277070651814117 53.328060376516994 13.27879191216694 14.116077059501949 50 18.437694904860297 53.60576169254945 15.78385290541082 12.172690497179433 51 18.682991926801137 53.754194265485246 14.498831006379815 13.063982801333804 52 19.943449280311082 49.936759361530875 15.760856309603867 14.358935048554175 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 2.0 8 4.0 9 14.5 10 25.0 11 95.0 12 165.0 13 1643.5 14 4143.0 15 5164.0 16 5054.5 17 4945.0 18 5336.0 19 5727.0 20 5418.0 21 5109.0 22 5400.0 23 5691.0 24 8295.5 25 10900.0 26 13363.5 27 15827.0 28 20234.5 29 24642.0 30 29471.0 31 34300.0 32 34739.5 33 35179.0 34 33186.5 35 31194.0 36 28931.5 37 26669.0 38 24933.5 39 22496.5 40 21795.0 41 23618.0 42 25441.0 43 23585.5 44 21730.0 45 23973.0 46 26216.0 47 28646.5 48 31077.0 49 30638.5 50 30200.0 51 29693.5 52 29187.0 53 31547.5 54 33908.0 55 32744.0 56 31580.0 57 29492.0 58 27404.0 59 27137.5 60 26871.0 61 20056.5 62 13242.0 63 11410.5 64 9421.5 65 9264.0 66 5705.0 67 2146.0 68 1685.0 69 1224.0 70 916.5 71 609.0 72 416.5 73 224.0 74 209.5 75 195.0 76 133.5 77 72.0 78 62.5 79 53.0 80 64.0 81 75.0 82 40.0 83 5.0 84 7.5 85 10.0 86 7.5 87 5.0 88 4.5 89 12.5 90 21.0 91 10.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 52 573998.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.712388398732905 #Duplication Level Percentage of deduplicated Percentage of total 1 86.46786292722618 36.067810824288564 2 5.7911808245137655 4.831279676788249 3 1.8156576224917378 2.2720624784548598 4 0.8696322761860916 1.4509775707339367 5 0.6064186134505888 1.2647584368236016 6 0.4355659972422594 1.0901098830150329 7 0.3620632944038529 1.0571767332768802 8 0.31031605291791337 1.0355218980539003 9 0.24380493940849005 0.915271769352283 >10 2.7638366611912466 23.46606431593258 >50 0.19944385625625735 5.642808638260824 >100 0.10703905073574098 8.836400434402176 >500 0.017561094261332507 5.241669370483034 >1k 0.009616789714539229 6.828087970134118 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGGTCGGTGGGGATTCTGCATGGGGCAAATCTTGAACATTCTTAGAAAGC 3461 0.6029637733929387 No Hit TATCGTCGGTGGGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTAT 3388 0.5902459590451534 No Hit ACGCGTCGGTGGGCCTGCCAAAGAGGCTGCCTGGGCAGTGGGGAAAAGCACA 3257 0.5674235798731007 No Hit GCAAGTCGGTGGGGTTGGACGCCTCGAACGTTGAGCAGGCTTTCCAAAACAT 2628 0.45784131652026666 No Hit CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCA 2413 0.4203847400165157 No Hit TAAGGTCGGTGGGGTTACAGTGAGCTATGATGGCAACACTGCACTCCAGCCT 2065 0.35975735107090967 No Hit CTTTGTCGGTGGGAGAGGCCAAGGTGGGCAGATCACCTGAGGTCAGGACTTC 2035 0.3545308520238747 No Hit TTGCGTCGGTGGGTGAGGCAAAGGTTGCAGTGAGCCGAGCCCGCACCACTGC 1864 0.3247398074557751 No Hit GTGGGTCGGTGGGTTGAAATAACCGCCGGGCGTGGTGGCTGATGCCTGTAAT 1684 0.29338081317356507 No Hit GGATGTCGGTGGGAAAGAGGATGGTTCAGAAAATTTTGTCGGATAAGGTTAT 1423 0.2479102714643605 No Hit TTAAGTCGGTGGGGAGTTCAAGACCAGCATGGTCAACATGGCAAAACCCTGT 1353 0.23571510702127882 No Hit GAATGTCGGTGGGGTGTGTGCTTGAGCTTTCTTTAAACTGCTCCAAATAGTA 1294 0.22543632556211 No Hit GTTCGTCGGTGGAGGGATTAAAGGCGTGAGCCATCGTGCCCTGCCTGAAACA 1244 0.2167254938170516 No Hit CTTAGTCGGTGGGCTGCGACACCGCATAAATCGCCGCAAGTTTTCAACCTGA 1237 0.21550597737274346 No Hit GTAGGTCGGTGGGGGTACAAAGTGCAAAGTGGCTGGTCTGGTGTTGTGCTTA 1192 0.20766622880219096 No Hit GTGAGTCGGTGGGAGCCCAATACAGCCAAATACTTATAGTCAACTGATCTTC 1163 0.2026139463900571 No Hit AGAGGTCGGTGGGGTCATTTTAAATAGCAATGAAAGAAAGACAGGGGAGAAA 1110 0.19338046474029524 No Hit AAGGGTCGGTGGGGGAGCTGGGATTAACAGTCCCACCTTTTGGCTTGGTCCT 1097 0.19111564848658008 No Hit TAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1092 0.19024456531207426 No Hit GTTAGTCGGTGGGAGTCTTGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGTG 1081 0.18832818232816143 No Hit GCTCGTCGGTGGGGGGAAATTAGGAAACCATAGCTACCTGAGTACTGAAATC 1049 0.1827532500113241 No Hit TTGTGTCGGTGGGCAACTCAGGACAATTAGGTGATTGGCGTTTGGGTAGAAT 1010 0.17595880125017857 No Hit CACTGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC 1010 0.17595880125017857 No Hit ATTTGTCGGTGGGTTCCTTTCCATTCCATTCCATTCCATTCCGTTCCATTCC 984 0.17142916874274822 No Hit GGTGGTCGGTGGATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTAT 983 0.17125495210784705 No Hit TTGGGTCGGTGGGGATCCTCGGCGTGATTGCGATTATCCTCGGCGTGCTGTT 978 0.17038386893334123 No Hit CACGGTCGGTGGGGTAGCCTTGTATTATAGTTTGAAGTCAGGTAGCATGATG 957 0.16672531960041673 No Hit GATGGTCGGTGGGTTGCTCAAAGCTCCACTCAGACCACTGTTGAATGTTTCC 936 0.16306677026749222 No Hit AAGCGTCGGTGGGAGAGTATTTGTCGGTGGGAGGGAACAGAGTTTGTGGCAA 925 0.1611503872835794 No Hit TGCCGTCGGTGGGGCTTTTCCATGCAAAGACTAGAAGTATTAAGGGTACTAT 912 0.1588855710298642 No Hit AAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 907 0.15801448785535838 No Hit GGCCGTCGGTGGGATAGCTTGCAGGCTAACATCCTGGGTCCAACCCCACATT 896 0.15609810487144554 No Hit AGGGGTCGGTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGG 879 0.15313642207812572 No Hit CGTAGTCGGTGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGC 858 0.1494778727452012 No Hit TTGAGTCGGTGGGTTGGACTATTTCAATATTTATAATTTCCCACTACATACT 842 0.14669040658678253 No Hit CAAGGTCGGTGGTAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCC 839 0.14616775668207904 No Hit TCGTGTCGGTGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCC 839 0.14616775668207904 No Hit ACACGTCGGTGGGCAGATGCTTTCATTTTAGATTTAGTTTTATTACAGATAG 827 0.14407715706326502 No Hit GCTAGTCGGTGGGTACGCAGAGACACCAGGAAACAGAGGAACGACACCAGCC 800 0.13937330792093353 No Hit ATTAGTCGGTGGGTACTACTCTTTTAAATAACATTTGTTTTTTTTCTAATTT 791 0.13780535820682302 No Hit TTTTGTCGGTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA 738 0.12857187655706118 No Hit TCTAGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC 732 0.12752657674765416 No Hit GTTCGTCGGTGGGGGTATTTCTCTTCAATGCGTGCCAGCGTACCGTCTTCAT 710 0.1236938107798285 No Hit TTTTGTCGGTGGGTCAATGTCATTGAGCCTTCTCATTATTTCAACTATTATT 696 0.12125477789121217 No Hit TCATGTCGGTGGGACTTTGGGAGGCCGAGGTGGTTCAATCGCCTGAGGTCAG 690 0.12020947808180515 No Hit TAAAGTCGGTGGGGGTTCTGACAGGCAGAGCAGAGGGAGCAGTTCCCCCTTT 667 0.11620249547907834 No Hit TCAGGTCGGTGGGACAACGGTTTTAGCACAGGTGTCATTAACTGCTGCGCGG 645 0.11236972951125265 No Hit GATCGTCGGTGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC 618 0.10766588036892114 No Hit GGGGGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 601 0.10470419757560132 No Hit CTGAGTCGGTGGGCAGAGTAGGAGTCGGTGGGGGAATTCCTGGAGATTGTCT 593 0.10331046449639197 No Hit GCTTGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 592 0.1031362478614908 No Hit TTAAGTCGGTGGGAACTAGCCTATCGCAGACGGTATCAACAGGAACATCCTC 592 0.1031362478614908 No Hit AAAGGTCGGTGGGAATCACGTCAGGGGTGGGAAATCACAGCCACATGCAGTT 575 0.10017456506817098 No Hit GACTGTCGGTGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG 574 0.1000003484332698 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.026306711870076202 0.0 0.0 0.0 0.0 12 0.061150038850309583 0.0 0.0 0.0 0.0 13 0.0979097488144558 0.0 0.0 0.0 0.0 14 0.40958330865264336 0.0 0.0 0.0 0.0 15 0.7907693058163966 0.0 0.0 0.0 0.0 16 1.1465196742845793 0.0 0.0 0.0 0.0 17 1.3383321893107643 0.0 0.0 0.0 0.0 18 1.4961724605312214 0.0 0.0 0.0 0.0 19 1.6541869483865796 0.0 0.0 0.0 0.0 20 1.8271840668434385 0.0 0.0 0.0 0.0 21 2.02300356447235 0.0 0.0 0.0 0.0 22 2.233631476067861 0.0 0.0 0.0 0.0 23 2.41115822703215 0.0 0.0 0.0 0.0 24 2.592517743964265 0.0 0.0 0.0 0.0 25 2.825445384827125 0.0 0.0 0.0 0.0 26 3.1237042637779227 0.0 0.0 0.0 0.0 27 3.396701730668051 0.0 0.0 0.0 0.0 28 3.651058017623755 0.0 0.0 0.0 0.0 29 4.00576308628253 0.0 0.0 0.0 0.0 30 4.47754173359489 0.0 0.0 0.0 0.0 31 4.852281715267301 0.0 0.0 0.0 0.0 32 5.112387151174743 0.0 0.0 0.0 0.0 33 5.357161523210882 0.0 0.0 0.0 0.0 34 5.58224941550319 0.0 0.0 0.0 0.0 35 5.813434890017039 0.0 0.0 0.0 0.0 36 6.0346900163415205 0.0 0.0 0.0 0.0 37 6.262739591427148 0.0 0.0 0.0 0.0 38 6.504378064035066 0.0 0.0 0.0 0.0 39 6.743229070484566 0.0 0.0 0.0 0.0 40 6.987829225885805 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCATG 35 1.0183794E-7 46.000004 17 CGTTTGG 75 0.0 46.000004 39 CGTCATA 165 0.0 46.000004 42 ATCCTAT 35 1.0183794E-7 46.000004 15 CATTGTA 140 0.0 46.000004 27 TCCTTAA 35 1.0183794E-7 46.000004 29 TTCACAC 165 0.0 46.000004 19 CCTATTT 35 1.0183794E-7 46.000004 17 ACTCGGG 35 1.0183794E-7 46.000004 23 TAAACTG 150 0.0 46.000004 34 CAGCATG 140 0.0 46.000004 25 TAGGAGG 35 1.0183794E-7 46.000004 36 TCGATTA 35 1.0183794E-7 46.000004 21 AGCTCCA 75 0.0 46.000004 22 CACACCG 165 0.0 46.000004 21 CCAGCTA 35 1.0183794E-7 46.000004 17 CTCCCGA 70 0.0 46.000004 35 AATTCAC 165 0.0 46.000004 17 CTGGGCA 325 0.0 46.000004 31 GGTCCCA 35 1.0183794E-7 46.000004 13 >>END_MODULE