SRR1528156_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528156_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	518570
Sequences flagged as poor quality	0
Sequence length	59
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
TATCGTCGGTGGGTGCCACTGCACTCCAGCCTGGGCGACAGAGCAAGACTATGTCTCAA	3202	0.6174672657500434	No Hit
CTTGGTCGGTGGGGATTCTGCATGGGGCAAATCTTGAACATTCTTAGAAAGCTGCAAGC	2800	0.5399463910368899	No Hit
ACGCGTCGGTGGGCCTGCCAAAGAGGCTGCCTGGGCAGTGGGGAAAAGCACAGATTCTG	2646	0.510249339529861	No Hit
GCAAGTCGGTGGGGTTGGACGCCTCGAACGTTGAGCAGGCTTTCCAAAACATCTTGACC	2374	0.4577974044005631	No Hit
TAAGGTCGGTGGGGTTACAGTGAGCTATGATGGCAACACTGCACTCCAGCCTGTGAGAC	1853	0.3573288080683418	No Hit
CGACGTCGGTGCCCGTTACGGCCTTTCCGTCGGCCACCTTACAGTCCTTGCACAGCTTG	1837	0.3542434001195595	No Hit
CTTTGTCGGTGGGAGAGGCCAAGGTGGGCAGATCACCTGAGGTCAGGACTTCAAGACTA	1741	0.3357309524268662	No Hit
TTGCGTCGGTGGGTGAGGCAAAGGTTGCAGTGAGCCGAGCCCGCACCACTGCCCTCCAG	1647	0.31760418072777064	No Hit
GTGGGTCGGTGGGTTGAAATAACCGCCGGGCGTGGTGGCTGATGCCTGTAATCCTAGCA	1291	0.24895385386736602	No Hit
TTAAGTCGGTGGGGAGTTCAAGACCAGCATGGTCAACATGGCAAAACCCTGTCTCTACT	1164	0.2244634282739071	No Hit
GTTCGTCGGTGGAGGGATTAAAGGCGTGAGCCATCGTGCCCTGCCTGAAACAATAACTT	1136	0.21906396436353817	No Hit
GAATGTCGGTGGGGTGTGTGCTTGAGCTTTCTTTAAACTGCTCCAAATAGTATGGAACT	1134	0.2186782883699404	No Hit
AGAGGTCGGTGGGGTCATTTTAAATAGCAATGAAAGAAAGACAGGGGAGAAAGAAACAG	1089	0.2100005785139904	No Hit
CTTAGTCGGTGGGCTGCGACACCGCATAAATCGCCGCAAGTTTTCAACCTGATCATGAA	1055	0.20344408662282815	No Hit
GTGAGTCGGTGGGAGCCCAATACAGCCAAATACTTATAGTCAACTGATCTTCAACAAAG	1045	0.20151570665483928	No Hit
GGATGTCGGTGGGAAAGAGGATGGTTCAGAAAATTTTGTCGGATAAGGTTATGAATGAA	1025	0.1976589467188615	No Hit
GCTCGTCGGTGGGGGGAAATTAGGAAACCATAGCTACCTGAGTACTGAAATCTATGATG	964	0.18589582891412926	No Hit
ATTTGTCGGTGGGTTCCTTTCCATTCCATTCCATTCCATTCCGTTCCATTCCATTCGTG	949	0.1830032589621459	No Hit
GATGGTCGGTGGGTTGCTCAAAGCTCCACTCAGACCACTGTTGAATGTTTCCAGGGAAG	936	0.18049636500376032	No Hit
CACGGTCGGTGGGGTAGCCTTGTATTATAGTTTGAAGTCAGGTAGCATGATGCCTCCAA	906	0.17471122509979367	No Hit
GTTAGTCGGTGGGAGTCTTGCTCTGTCACCCAGCCTGGAGTGCAGTGGCGTGATCTCAG	884	0.1704687891702181	No Hit
AAGGGTCGGTGGGGGAGCTGGGATTAACAGTCCCACCTTTTGGCTTGGTCCTTTACGGG	875	0.1687332471990281	No Hit
TAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	871	0.16796189521183255	No Hit
CACTGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA	826	0.1592841853558825	No Hit
GGTGGTCGGTGGATAACATCTTCAGTCATTGTAGTCTGACAGGCAAAATTATTATTGCT	826	0.1592841853558825	No Hit
CAAGGTCGGTGGTAAGGTATACTGCAAATATTTCCTCCCGACTCAAGCATCCAGTCATA	824	0.15889850936228475	No Hit
TTGTGTCGGTGGGCAACTCAGGACAATTAGGTGATTGGCGTTTGGGTAGAATCTCGAAT	815	0.15716296739109473	No Hit
AAGCGTCGGTGGGAGAGTATTTGTCGGTGGGAGGGAACAGAGTTTGTGGCAAGAGAAAC	756	0.14578552557996027	No Hit
TTGAGTCGGTGGGTTGGACTATTTCAATATTTATAATTTCCCACTACATACTGGCATCC	736	0.1419287656439825	No Hit
GCTAGTCGGTGGGTACGCAGAGACACCAGGAAACAGAGGAACGACACCAGCCAGGAACC	729	0.14057889966639028	No Hit
CGTAGTCGGTGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCGG	721	0.13903619569199915	No Hit
GGCCGTCGGTGGGATAGCTTGCAGGCTAACATCCTGGGTCCAACCCCACATTGTAACTT	719	0.13865051969840136	No Hit
ACACGTCGGTGGGCAGATGCTTTCATTTTAGATTTAGTTTTATTACAGATAGAATGAGA	719	0.13865051969840136	No Hit
AGGGGTCGGTGGGTCATGCGGGAGATGTATTGTTAGCCATTTCCACCCGTGGCAACAGC	702	0.13537227375282024	No Hit
TGCCGTCGGTGGGGCTTTTCCATGCAAAGACTAGAAGTATTAAGGGTACTATCAATAAC	696	0.13421524577202693	No Hit
TTGGGTCGGTGGGGATCCTCGGCGTGATTGCGATTATCCTCGGCGTGCTGTTTGAGAAT	694	0.13382956977842914	No Hit
AAAAGTCGGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	689	0.13286537979443472	No Hit
TCGTGTCGGTGGGATTTGAGGAAAACTGCGACTGTTCTTTAACCAAACATCCGTGCGAT	667	0.12862294386485912	No Hit
ATTAGTCGGTGGGTACTACTCTTTTAAATAACATTTGTTTTTTTTCTAATTTACAAATA	639	0.12322347995449023	No Hit
TTTTGTCGGTGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG	601	0.11589563607613246	No Hit
TCTAGTCGGTGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA	551	0.10625373623618797	No Hit
TCATGTCGGTGGGACTTTGGGAGGCCGAGGTGGTTCAATCGCCTGAGGTCAGGAGTTCA	526	0.10143278631621575	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGTTTC	30	7.0029455E-7	53.000004	50
GTCATTT	110	0.0	53.000004	14
CGGGTTA	30	7.0029455E-7	53.000004	17
ATCCTAG	150	0.0	53.000004	51
ATCCTAC	15	0.0066942032	53.000004	28
TGTTTAA	30	7.0029455E-7	53.000004	48
AGACTTT	30	7.0029455E-7	53.000004	36
GAAATCT	60	0.0	53.000004	47
AGCGTGA	30	7.0029455E-7	53.000004	15
CTAGAAG	60	0.0	53.000004	31
TGGAGTA	15	0.0066942032	53.000004	10
AGCAACT	30	7.0029455E-7	53.000004	51
ACAGTTA	15	0.0066942032	53.000004	28
GATACCT	15	0.0066942032	53.000004	13
CTGTCCC	15	0.0066942032	53.000004	53
CTCACGC	55	0.0	53.000004	22
GTACCCA	15	0.0066942032	53.000004	13
AATTTTA	30	7.0029455E-7	53.000004	20
ATCTATG	60	0.0	53.000004	50
TACCATC	15	0.0066942032	53.000004	28

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content