FastQCFastQC Report
Fri 17 Jun 2016
SRR1528152_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528152_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3394910
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTGTGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGACCTGCG38470.1133167005900009No Hit
GGAAGCTGTGGAGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACT35460.10445048616900005No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGCC405.6170393E-946.00000424
ACAACTA206.3132046E-446.00000419
GTCATTC551.8189894E-1246.00000417
TTTAGTG800.046.00000443
CTTAACC351.0203621E-746.00000433
ATCCTAC206.3132046E-446.00000422
CATATTA551.8189894E-1246.00000432
ATGATTT206.3132046E-446.00000437
TTAATTG700.046.00000420
TCACCGA206.3132046E-446.00000439
CTCAATA1100.046.00000416
TTAATCT351.0203621E-746.00000429
CGTATAT2200.046.00000440
ATAGGTA405.6170393E-946.00000433
TACCATA351.0203621E-746.00000434
GATAAAC700.046.00000422
ATCTAAC206.3132046E-446.00000434
CATGCAC206.3132046E-446.00000434
GACACGT551.8189894E-1246.00000420
ACACCTA700.046.00000420
Produced by FastQC (version 0.11.5)