##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528152_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3394910
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.700453325714083	31.0	31.0	33.0	28.0	34.0
2	31.488805594257286	31.0	31.0	34.0	30.0	34.0
3	31.809266225025112	31.0	31.0	34.0	30.0	34.0
4	35.489236533516355	37.0	35.0	37.0	33.0	37.0
5	35.409896580468995	37.0	35.0	37.0	33.0	37.0
6	35.538782471405725	37.0	35.0	37.0	33.0	37.0
7	35.59601550556569	37.0	35.0	37.0	33.0	37.0
8	35.594711494560975	37.0	35.0	37.0	33.0	37.0
9	35.388574365741654	37.0	34.0	39.0	30.0	39.0
10	36.44551843789673	37.0	35.0	39.0	32.0	39.0
11	37.44170007452333	39.0	37.0	39.0	35.0	39.0
12	37.57532335172361	39.0	37.0	39.0	35.0	39.0
13	37.38131290667499	39.0	37.0	39.0	35.0	39.0
14	37.238784827874674	40.0	37.0	40.0	31.0	40.0
15	35.97063957512865	38.0	35.0	40.0	30.0	40.0
16	36.4767219160449	38.0	35.0	40.0	31.0	40.0
17	36.29359158269291	38.0	35.0	40.0	30.0	40.0
18	36.624489603553556	38.0	35.0	40.0	31.0	40.0
19	36.747603029240835	39.0	35.0	40.0	31.0	40.0
20	36.89677075386387	39.0	35.0	40.0	31.0	40.0
21	36.86457549684675	39.0	35.0	40.0	31.0	40.0
22	37.032371697629685	39.0	36.0	40.0	31.0	40.0
23	37.05512311077466	39.0	36.0	40.0	31.0	40.0
24	36.86406296485032	39.0	35.0	40.0	31.0	40.0
25	36.95597762532733	39.0	36.0	40.0	31.0	40.0
26	36.47184225796855	39.0	35.0	40.0	30.0	40.0
27	35.88972991920257	38.0	35.0	40.0	30.0	40.0
28	35.37639201039203	37.0	34.0	40.0	29.0	40.0
29	35.30033756417696	37.0	34.0	40.0	29.0	40.0
30	35.20451558362372	37.0	34.0	40.0	28.0	40.0
31	35.03632732531938	37.0	33.0	40.0	28.0	40.0
32	34.79200833011774	37.0	33.0	40.0	26.0	40.0
33	34.6718478545823	37.0	33.0	40.0	25.0	40.0
34	34.45529248198038	36.0	33.0	40.0	24.0	40.0
35	34.28803267244198	36.0	33.0	40.0	23.0	40.0
36	33.92038581287869	35.0	33.0	40.0	22.0	40.0
37	33.47882830472678	35.0	33.0	40.0	21.0	40.0
38	32.858911134610345	35.0	33.0	39.0	18.0	40.0
39	32.17452450874986	35.0	32.0	39.0	15.0	40.0
40	31.484699447113474	35.0	31.0	38.0	12.0	40.0
41	30.738108815844896	35.0	29.0	38.0	10.0	40.0
42	30.033948175356638	35.0	26.0	37.0	9.0	40.0
43	29.428405465829726	35.0	23.0	37.0	8.0	40.0
44	28.938314123202087	35.0	22.0	36.0	8.0	40.0
45	28.580209490089576	34.0	22.0	35.0	8.0	40.0
46	28.216806631103623	34.0	20.0	35.0	8.0	39.0
47	27.69950720343102	33.0	20.0	35.0	8.0	39.0
48	27.26627480551767	33.0	18.0	35.0	8.0	39.0
49	26.939133879837758	33.0	15.0	35.0	8.0	39.0
50	26.52613648079036	33.0	15.0	35.0	8.0	38.0
51	26.074093274932178	33.0	12.0	35.0	8.0	37.0
52	25.15825898182868	32.0	10.0	35.0	8.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	6.0
14	14.0
15	73.0
16	252.0
17	675.0
18	1930.0
19	4445.0
20	8753.0
21	15676.0
22	24647.0
23	36545.0
24	51602.0
25	70322.0
26	91851.0
27	117385.0
28	147031.0
29	175864.0
30	189539.0
31	176860.0
32	167742.0
33	190476.0
34	249363.0
35	317581.0
36	411762.0
37	537841.0
38	360312.0
39	46358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.507274125087264	23.554144292484928	34.65977006754229	6.27881151488552
2	20.0941998462404	23.193309984653496	33.11215908521876	23.600331083887347
3	20.80714952679158	21.25947374157194	35.37006872052573	22.56330801111075
4	24.855828284107677	19.102803903490813	38.643115723244506	17.398252089157
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.2172693826935	0.4664924843368455	0.22162590466315749	0.09461222830649414
12	98.36390360863764	0.8466792934127856	0.30133346686657375	0.4880836310830037
13	91.5807488269203	5.333131069748536	1.5651372201324927	1.5209828831986711
14	36.40429348642527	43.02635415960953	10.235529071462866	10.333823282502335
15	21.753301265718385	51.249105278195884	18.28675870641637	8.710834749669358
16	32.1890712861313	39.791305218695044	17.36723506661443	10.652388428559226
17	19.05429009900115	38.93522950534771	23.112924937627213	18.897555458023923
18	19.988777316629896	41.15499379954108	23.032451523015336	15.82377736081369
19	22.779396213743517	39.92126448123809	24.763631436474014	12.53570786854438
20	24.09639725353544	40.24963842929562	21.86449714425419	13.789467172914746
21	24.559031019968128	37.899001740841435	18.107284140080296	19.43468309911014
22	28.747036003899957	38.27709718372505	19.504228388970546	13.47163842340445
23	24.704307330680344	39.18239364224678	16.7241252345423	19.38917379253058
24	22.594118842620276	40.7420520720726	21.94346830991101	14.720360775396108
25	29.921411760547407	37.86536314659299	18.101510791154993	14.11171430170461
26	25.811641545725806	39.98159008633513	20.368551743639742	13.838216624299319
27	33.217581614829264	41.758603320853865	14.443740776633254	10.58007428768362
28	31.70646644535496	41.81621898665944	14.663540417860856	11.813774150124745
29	31.038672601040968	44.35675761654948	11.85454106294423	12.750028719465318
30	29.516923865433842	48.85195778385878	11.802728201925824	9.828390148781558
31	21.933512228601053	54.19313030389613	12.095931850917992	11.777425616584829
32	19.988011464221437	59.142952243211155	11.697217304729728	9.171818987837675
33	19.896285910377596	59.94671434588841	10.429584289421516	9.727415454312485
34	16.06027847571806	61.24645425062815	11.1503397733666	11.542927500287195
35	18.346760297032912	59.79425080488142	11.76608510976727	10.092903788318393
36	17.35056304879953	60.277709865651815	12.85115069324371	9.520576392304951
37	17.834257756464826	59.726325587423524	13.026236336162079	9.413180319949571
38	17.993231042943698	58.9254796150708	13.687432067418575	9.393857274566924
39	18.768244224441887	58.64709226459611	12.685196367503115	9.899467143458885
40	21.20521604401884	57.836525857828335	11.503574468837172	9.454683629315653
41	22.953097431154287	55.94274958688153	10.934192659010106	10.169960322954068
42	20.528231970803347	55.61708557811548	11.895072328868807	11.95961012221237
43	21.741253818216094	53.900073934213275	13.120171079645704	11.238501167924923
44	22.8038151232286	53.16942128068196	12.631675066496609	11.395088529592831
45	21.448433095428154	53.14847816289681	13.353962255258608	12.04912648641643
46	23.171365367564974	53.292517327410735	11.601633032981729	11.934484272042557
47	22.78269527027226	53.17095298549888	11.602516708837642	12.443835035391219
48	22.128568945863073	53.243826787749896	11.49959792748556	13.128006338901473
49	21.920021443867437	54.31166069203602	11.72941256174685	12.038905302349693
50	20.252790206515048	54.83576884217843	11.808943388779083	13.102497562527432
51	19.552653825874618	54.36238368616546	12.072426073150687	14.01253641480923
52	22.147567976765217	51.0496301816543	13.471284953062085	13.331516888518399
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	2.0
8	4.0
9	27.5
10	51.0
11	591.5
12	1132.0
13	5847.0
14	23440.0
15	36318.0
16	43237.0
17	50156.0
18	45200.0
19	40244.0
20	34772.5
21	29301.0
22	33330.0
23	37359.0
24	44388.5
25	51418.0
26	64182.0
27	76946.0
28	94293.5
29	111641.0
30	133768.5
31	155896.0
32	174439.0
33	192982.0
34	207868.5
35	222755.0
36	226050.5
37	229346.0
38	221586.5
39	196756.0
40	179685.0
41	168275.0
42	156865.0
43	147602.0
44	138339.0
45	139446.0
46	140553.0
47	137077.5
48	133602.0
49	142358.5
50	151115.0
51	151346.0
52	151577.0
53	155418.0
54	159259.0
55	150261.0
56	141263.0
57	135888.0
58	130513.0
59	129296.0
60	128079.0
61	118253.0
62	108427.0
63	96610.0
64	67221.0
65	49649.0
66	39360.5
67	29072.0
68	24652.0
69	20232.0
70	15742.0
71	11252.0
72	10091.5
73	8931.0
74	8126.5
75	7322.0
76	4984.5
77	2647.0
78	1746.5
79	846.0
80	453.0
81	60.0
82	350.5
83	641.0
84	401.5
85	162.0
86	100.0
87	38.0
88	28.0
89	22.5
90	27.0
91	15.5
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3394910.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.17124865597776
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.93661201110973	35.79288870417662
2	5.787776489127577	4.765799699981868
3	1.7121615445994645	2.1147548607572255
4	0.8379666800430788	1.3800053819791103
5	0.5160868762369444	1.0623970554819029
6	0.37469148643451783	0.9255909814364073
7	0.2853113017088022	0.8222635782909653
8	0.23132052665785657	0.7619003937809876
9	0.19922893691797916	0.7382253691184606
>10	2.789706685034006	24.61829788967961
>50	0.21234115043675064	5.824204873840143
>100	0.0820581210597197	7.266773914834723
>500	0.02274173920722365	6.687586900720656
>1k	0.011996451426427631	7.239310395921299
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTGCTGTGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGACCTGCG	3847	0.1133167005900009	No Hit
GGAAGCTGTGGAGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACT	3546	0.10445048616900005	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.027040481190959406	0.0	0.0	0.0	0.0
12	0.05820478304284944	0.0	0.0	0.0	0.0
13	0.1887826186850314	0.0	0.0	0.0	0.0
14	0.5988671275527186	0.0	0.0	0.0	0.0
15	0.9183454053273872	0.0	0.0	0.0	0.0
16	1.1340801376177867	0.0	0.0	0.0	0.0
17	1.2724932325157368	0.0	0.0	0.0	0.0
18	1.4472254050917404	0.0	0.0	0.0	0.0
19	1.6065816177748453	0.0	0.0	0.0	0.0
20	1.7540671181268428	0.0	0.0	0.0	0.0
21	1.910742847380343	0.0	0.0	0.0	0.0
22	2.0923087799087456	0.0	0.0	0.0	0.0
23	2.2948178302223035	0.0	0.0	0.0	0.0
24	2.5191536741769296	0.0	0.0	0.0	0.0
25	2.768055706925957	0.0	0.0	0.0	0.0
26	3.033364654733115	0.0	0.0	0.0	0.0
27	3.279350557157627	0.0	0.0	0.0	0.0
28	3.536588598814107	0.0	0.0	0.0	0.0
29	3.8226344733733737	0.0	0.0	0.0	0.0
30	4.129299451237293	0.0	0.0	0.0	0.0
31	4.433696327737701	0.0	0.0	0.0	0.0
32	4.69729683555676	0.0	0.0	0.0	0.0
33	4.94466716348887	0.0	0.0	0.0	0.0
34	5.186352510081269	0.0	0.0	0.0	0.0
35	5.413162646432453	0.0	0.0	0.0	0.0
36	5.6480731447961805	0.0	0.0	0.0	0.0
37	5.8899352265597615	0.0	0.0	0.0	0.0
38	6.136775348978324	0.0	0.0	0.0	0.0
39	6.403940016082901	0.0	0.0	0.0	0.0
40	6.679470147956794	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGCC	40	5.6170393E-9	46.000004	24
ACAACTA	20	6.3132046E-4	46.000004	19
GTCATTC	55	1.8189894E-12	46.000004	17
TTTAGTG	80	0.0	46.000004	43
CTTAACC	35	1.0203621E-7	46.000004	33
ATCCTAC	20	6.3132046E-4	46.000004	22
CATATTA	55	1.8189894E-12	46.000004	32
ATGATTT	20	6.3132046E-4	46.000004	37
TTAATTG	70	0.0	46.000004	20
TCACCGA	20	6.3132046E-4	46.000004	39
CTCAATA	110	0.0	46.000004	16
TTAATCT	35	1.0203621E-7	46.000004	29
CGTATAT	220	0.0	46.000004	40
ATAGGTA	40	5.6170393E-9	46.000004	33
TACCATA	35	1.0203621E-7	46.000004	34
GATAAAC	70	0.0	46.000004	22
ATCTAAC	20	6.3132046E-4	46.000004	34
CATGCAC	20	6.3132046E-4	46.000004	34
GACACGT	55	1.8189894E-12	46.000004	20
ACACCTA	70	0.0	46.000004	20
>>END_MODULE