FastQCFastQC Report
Fri 17 Jun 2016
SRR1528151_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528151_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379202
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGCTGTGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGACCTGCG38200.11304444066972023No Hit
GGAAGCTGTGGAGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACT34350.10165121824620132No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTTA1400.046.00000424
ATGATTA351.0203621E-746.00000430
GCGACGA351.0203621E-746.00000417
ATAGGTA351.0203621E-746.00000433
TACTTAT700.046.00000423
TCCTGTA700.046.00000418
TCATTCA700.046.00000418
TTTGTCC700.046.00000415
TAGGTAA351.0203621E-746.00000434
CACATAA700.046.00000446
CGGTTAT351.0203621E-746.00000424
CCTACAT700.046.00000424
CGATTAA700.046.00000435
CATTCGT1450.046.00000435
CCCACTA351.0203621E-746.00000415
ACCTCCG1450.046.00000446
CGTTAAT351.0203621E-746.00000424
ACGATTA700.046.00000434
GTAGCCA351.0203621E-746.00000444
CCATCTA700.046.00000416
Produced by FastQC (version 0.11.5)