##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528151_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3379202
Sequences flagged as poor quality	0
Sequence length	52
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.72467937696533	31.0	31.0	33.0	28.0	34.0
2	31.50943921079592	31.0	31.0	34.0	30.0	34.0
3	31.825920439204285	31.0	31.0	34.0	30.0	34.0
4	35.52313859899468	37.0	35.0	37.0	33.0	37.0
5	35.615744190492315	37.0	35.0	37.0	33.0	37.0
6	35.6073442783237	37.0	35.0	37.0	33.0	37.0
7	35.5936709317762	37.0	35.0	37.0	33.0	37.0
8	35.59687819787038	37.0	35.0	37.0	33.0	37.0
9	35.37157411720282	37.0	34.0	39.0	30.0	39.0
10	36.38982724323672	37.0	35.0	39.0	32.0	39.0
11	37.415904997688806	39.0	37.0	39.0	35.0	39.0
12	37.58912725548813	39.0	37.0	39.0	35.0	39.0
13	37.37899657966585	39.0	37.0	39.0	35.0	39.0
14	37.20877650995708	40.0	37.0	40.0	31.0	40.0
15	36.012009048290096	38.0	35.0	40.0	30.0	40.0
16	36.47787110684712	38.0	35.0	40.0	31.0	40.0
17	36.314372742440376	38.0	35.0	40.0	30.0	40.0
18	36.561046661312346	38.0	35.0	40.0	30.0	40.0
19	36.751635741219374	39.0	35.0	40.0	31.0	40.0
20	36.91176023214948	39.0	35.0	40.0	31.0	40.0
21	36.925251583066064	39.0	36.0	40.0	31.0	40.0
22	36.97417851907048	39.0	36.0	40.0	31.0	40.0
23	37.07503369138631	39.0	36.0	40.0	31.0	40.0
24	37.00685753618754	39.0	36.0	40.0	31.0	40.0
25	37.01437114442996	39.0	36.0	40.0	31.0	40.0
26	36.405638372609864	39.0	35.0	40.0	30.0	40.0
27	35.79939908889732	38.0	35.0	40.0	29.0	40.0
28	35.335409957735585	37.0	34.0	40.0	29.0	40.0
29	35.25489864175033	37.0	34.0	40.0	29.0	40.0
30	35.025488858020324	37.0	33.0	40.0	28.0	40.0
31	34.923297275510606	37.0	33.0	40.0	27.0	40.0
32	34.76970006528168	37.0	33.0	40.0	26.0	40.0
33	34.74175323049643	37.0	33.0	40.0	25.0	40.0
34	34.578547538738434	37.0	33.0	40.0	25.0	40.0
35	34.23322547749439	36.0	33.0	40.0	23.0	40.0
36	33.901252721796446	35.0	33.0	40.0	22.0	40.0
37	33.46022285734916	35.0	33.0	40.0	21.0	40.0
38	32.89368288726155	35.0	33.0	39.0	18.0	40.0
39	32.20983149276071	35.0	32.0	39.0	15.0	40.0
40	31.452779088080558	35.0	31.0	38.0	12.0	40.0
41	30.68595337005601	35.0	29.0	38.0	10.0	40.0
42	29.986132524779517	35.0	26.0	37.0	9.0	40.0
43	29.421457196107248	35.0	23.0	37.0	8.0	40.0
44	28.850628343614854	34.0	22.0	36.0	8.0	40.0
45	28.489952065606023	34.0	21.0	35.0	8.0	40.0
46	28.11442168890762	34.0	20.0	35.0	8.0	39.0
47	27.71467257654322	33.0	20.0	35.0	8.0	39.0
48	27.28001403881745	33.0	18.0	35.0	8.0	39.0
49	26.78971544169304	33.0	15.0	35.0	8.0	39.0
50	26.376149753699245	33.0	13.0	35.0	8.0	38.0
51	25.943338989501072	33.0	11.0	35.0	8.0	37.0
52	24.886018059885146	31.0	10.0	35.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	0.0
12	3.0
13	10.0
14	20.0
15	71.0
16	208.0
17	704.0
18	1894.0
19	4402.0
20	9096.0
21	15364.0
22	24747.0
23	36343.0
24	51672.0
25	70427.0
26	92470.0
27	117714.0
28	146729.0
29	175719.0
30	189598.0
31	175401.0
32	168172.0
33	191290.0
34	248722.0
35	314271.0
36	408652.0
37	535145.0
38	358911.0
39	41445.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.58491028355215	23.548251924566806	34.576832044962096	6.290005746918947
2	20.12697080553338	23.16990816174943	33.114386177564995	23.588734855152193
3	20.736966893367132	21.270317666715396	35.42519802012428	22.56751741979319
4	24.87463016416302	19.06967976463082	38.68700361801396	17.368686453192204
5	100.0	0.0	0.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	100.0	0.0	0.0	0.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	99.21806982832041	0.4657608512305568	0.22123566451487658	0.0949336559341525
12	98.3806235910135	0.8313205307051784	0.29731871607557053	0.49073716220575153
13	91.5854689953427	5.334395517048108	1.563416451576437	1.5167190360327674
14	36.30357107979931	43.15705305572144	10.22700033913332	10.312375525345924
15	21.743595085466925	51.348720792660515	18.180386967100517	8.727297154772044
16	32.2037273888924	39.83839971685622	17.34308869372118	10.614784200530185
17	19.008541069755523	38.98769591163831	23.109893992723727	18.89386902588244
18	19.96098487157619	41.24846635389065	22.981254154087267	15.809294620445893
19	22.785290728402742	39.9926077221782	24.71367500374349	12.508426545675578
20	24.047215881145902	40.336535075440885	21.83145606566284	13.784792977750367
21	24.585567835246312	37.93895718574977	18.03715788520485	19.438317093799068
22	28.6954138876575	38.317034613497505	19.552456467532867	13.435095031312127
23	24.583348376332637	39.278326658187346	16.801955017782305	19.33636994769771
24	22.622471222495726	40.82608852622601	21.84486159750142	14.70657865377684
25	29.88288359204333	37.87337957304713	18.11395116361792	14.129785671291625
26	25.71962256177642	40.05182288599497	20.340068454031453	13.888486098197147
27	33.16993183597784	41.76119095573452	14.450689837423155	10.618187370864481
28	31.654574068078794	41.880597845290104	14.660206758873839	11.804621327757262
29	30.972164434088285	44.40696353754525	11.90935019569709	12.71152183266937
30	29.48841176111993	48.81978052806551	11.891505746031164	9.800301964783403
31	21.910380024633035	54.21214831193873	12.087173243860533	11.790298419567696
32	19.975219001409208	59.188382345891135	11.6598830138003	9.176515638899362
33	19.840867755168233	60.05672345127636	10.34744297618195	9.754965817373451
34	16.0644732099472	61.38076386081684	11.025502470701664	11.529260458534294
35	18.25138005955252	59.7845881956746	11.912960515529997	10.051071229242881
36	17.356760560629404	60.31959616501174	12.84705679033097	9.476586484027887
37	17.794082744979438	59.8074930116637	13.013042724288159	9.385381519068703
38	18.000196496095825	58.98528705889734	13.656153139113908	9.35836330589293
39	18.814797102984667	58.65665917574623	12.633396878908098	9.895146842361006
40	21.219477261199536	57.83764924381555	11.525590953130354	9.417282541854556
41	23.017978800912168	55.88570911120436	10.924265551452681	10.17204653643079
42	20.57302286161052	55.59007718390318	11.851111593802324	11.985788360683971
43	21.81417387892171	53.8111660682019	13.143931614623808	11.230728438252582
44	22.798903409739932	53.126536975297725	12.744429010162756	11.330130604799594
45	21.44515184354176	53.124495073097144	13.403105230169727	12.027247853191374
46	23.20157244225116	53.327531174519905	11.53266362886859	11.93823275436035
47	22.883035698960878	53.218363388752735	11.500644234940676	12.397956677345716
48	22.153810278284634	53.332502762486534	11.354396688922415	13.15929027030642
49	21.856491562209065	54.2270334830531	11.914854453802999	12.001620500934836
50	20.26697427380784	54.8390122875164	11.825129128119597	13.068884310556161
51	19.533635455944925	54.38011696252547	12.103686018178255	13.982561563351348
52	22.347998136838225	50.85603050661073	13.53414208443295	13.261829272118092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	1.0
8	2.0
9	19.0
10	36.0
11	530.0
12	1024.0
13	5685.0
14	23309.5
15	36273.0
16	42968.5
17	49664.0
18	44864.5
19	40065.0
20	34515.5
21	28966.0
22	32948.5
23	36931.0
24	44021.5
25	51112.0
26	63683.5
27	76255.0
28	93611.0
29	110967.0
30	133646.5
31	156326.0
32	174672.5
33	193019.0
34	207572.5
35	222126.0
36	225276.5
37	228427.0
38	221413.0
39	197203.0
40	180007.0
41	167868.5
42	155730.0
43	146493.5
44	137257.0
45	138534.5
46	139812.0
47	136117.5
48	132423.0
49	141587.0
50	150751.0
51	150693.5
52	150636.0
53	154116.5
54	157597.0
55	148894.0
56	140191.0
57	135410.5
58	130630.0
59	128845.0
60	127060.0
61	117304.5
62	107549.0
63	95734.5
64	66501.0
65	49082.0
66	39029.5
67	28977.0
68	24455.0
69	19933.0
70	15667.0
71	11401.0
72	10124.0
73	8847.0
74	7965.0
75	7083.0
76	4860.0
77	2637.0
78	1719.5
79	802.0
80	433.0
81	64.0
82	337.0
83	610.0
84	389.0
85	168.0
86	112.5
87	57.0
88	40.5
89	19.5
90	15.0
91	8.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	3379202.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.4240346526618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.94372573222104	36.015599075630476
2	5.811569331209815	4.814772987247639
3	1.6953869398920716	2.106893020432783
4	0.8301606674804859	1.3755441702795401
5	0.5032310174672742	1.042292955292931
6	0.3628830065665089	0.90192469433239
7	0.3014730582009074	0.8741761286830824
8	0.24230416746978822	0.8029772983802301
9	0.21722584655473764	0.8098533895623523
>10	2.76584200857793	24.506276970753
>50	0.21017971836810476	5.775173636867728
>100	0.08165946598204503	7.233891698236306
>500	0.022589241197752484	6.626717406847214
>1k	0.011769798811732627	7.113906567454322
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTGCTGTGGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGACCTGCG	3820	0.11304444066972023	No Hit
GGAAGCTGTGGAGGGGCTGGTGAGATGGCTCAGTGAGTAAGAGCACCCGACT	3435	0.10165121824620132	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02766925445711739	0.0	0.0	0.0	0.0
12	0.06075398866359572	0.0	0.0	0.0	0.0
13	0.19537156997421284	0.0	0.0	0.0	0.0
14	0.5987804221233297	0.0	0.0	0.0	0.0
15	0.9202764439651728	0.0	0.0	0.0	0.0
16	1.1343802471707818	0.0	0.0	0.0	0.0
17	1.2706550244702743	0.0	0.0	0.0	0.0
18	1.4427962578147149	0.0	0.0	0.0	0.0
19	1.6004074334709792	0.0	0.0	0.0	0.0
20	1.744820226787271	0.0	0.0	0.0	0.0
21	1.8991466032513002	0.0	0.0	0.0	0.0
22	2.07732476484093	0.0	0.0	0.0	0.0
23	2.272933077099268	0.0	0.0	0.0	0.0
24	2.492333988912175	0.0	0.0	0.0	0.0
25	2.736740804485793	0.0	0.0	0.0	0.0
26	2.9927183991960233	0.0	0.0	0.0	0.0
27	3.243961148223752	0.0	0.0	0.0	0.0
28	3.5001162996470763	0.0	0.0	0.0	0.0
29	3.7834968137447835	0.0	0.0	0.0	0.0
30	4.090226035614325	0.0	0.0	0.0	0.0
31	4.387781493974021	0.0	0.0	0.0	0.0
32	4.650920542779035	0.0	0.0	0.0	0.0
33	4.893522198436199	0.0	0.0	0.0	0.0
34	5.133016611614221	0.0	0.0	0.0	0.0
35	5.360141240446709	0.0	0.0	0.0	0.0
36	5.593421168666448	0.0	0.0	0.0	0.0
37	5.830962458000439	0.0	0.0	0.0	0.0
38	6.074540675579619	0.0	0.0	0.0	0.0
39	6.331287682713256	0.0	0.0	0.0	0.0
40	6.606175067367976	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCTTA	140	0.0	46.000004	24
ATGATTA	35	1.0203621E-7	46.000004	30
GCGACGA	35	1.0203621E-7	46.000004	17
ATAGGTA	35	1.0203621E-7	46.000004	33
TACTTAT	70	0.0	46.000004	23
TCCTGTA	70	0.0	46.000004	18
TCATTCA	70	0.0	46.000004	18
TTTGTCC	70	0.0	46.000004	15
TAGGTAA	35	1.0203621E-7	46.000004	34
CACATAA	70	0.0	46.000004	46
CGGTTAT	35	1.0203621E-7	46.000004	24
CCTACAT	70	0.0	46.000004	24
CGATTAA	70	0.0	46.000004	35
CATTCGT	145	0.0	46.000004	35
CCCACTA	35	1.0203621E-7	46.000004	15
ACCTCCG	145	0.0	46.000004	46
CGTTAAT	35	1.0203621E-7	46.000004	24
ACGATTA	70	0.0	46.000004	34
GTAGCCA	35	1.0203621E-7	46.000004	44
CCATCTA	70	0.0	46.000004	16
>>END_MODULE