FastQCFastQC Report
Fri 17 Jun 2016
SRR1528150_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528150_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3068150
Sequences flagged as poor quality0
Sequence length59
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCTA503.6379788E-1253.00000447
CGTATAA251.47931605E-553.00000450
CCAGGTA251.47931605E-553.00000447
CTATAAT251.47931605E-553.00000420
ACTTACG503.6379788E-1253.00000420
GCATTTA503.6379788E-1253.00000448
CCACTGA1000.053.00000452
ACGTGCC1000.053.00000452
GTACTTC650.053.00000418
ATAAGGT503.6379788E-1253.00000435
CATACTT3100.053.00000445
ACGTCCC251.47931605E-553.00000437
ACCACCG1550.053.00000425
CTATTGA1000.053.00000434
CGTCACA650.053.00000444
CTTAATA457.6397555E-1153.029
ATTTACA150.006697326453.051
TTTAGAC850.053.030
TGATATA600.053.040
GATACGT307.0143506E-753.020