FastQCFastQC Report
Fri 17 Jun 2016
SRR1528149_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528149_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1512656
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGTTTGGTGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAG39550.26146063612612513No Hit
ATAGGGGTTTGGGACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCA38000.2512137591098042No Hit
CCAGGGGTTTGGGGGGAGGCCGAGGTGGGCGGATCATGAAGTCAGGAGATCG28160.1861626172771602No Hit
TCTTGGGTTTAGGAGGCCTCTGTACCTTCCAAGGGGCTCCCCCCTCTGCTCT25740.17016426735490423No Hit
CATAGGGTTTGGGCGAGGTCAGGAGATCGAGGCCATCCCGGCTAAAACGGTG25710.16996594070297544No Hit
GATAGGGTTTGGGAACATTACAGGGCTTGCCTCAAATCTCTGAAAAGCCACA24470.16176843908991867No Hit
CTCAGGGTTTGGGCTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACC23450.1550253329243397No Hit
GATGGGGTTTCCCAGGACTGGGCTAGGGAGTGTTGGTCTAGATGCAAACCTA22180.14662950465935415No Hit
GGCAGGGTTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAG21380.14134079394125298No Hit
TATGGGGTTTGGGGTTGCTGGGTTTGGGAAAACCCTCACTAGTGACTGAAGA19610.12963952147745422No Hit
TCAAGGGTTTGGGGCCGGCGTTGTGGCGCACGCCTTTAATCCCAGCATTGGG18810.12435081075935309No Hit
CCAGGGGTTTGGGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCG17970.11879766450534689No Hit
GACTGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA17860.11807046678160799No Hit
TCTTGGGTTTGGGTACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGG17730.11721105128991655No Hit
ACTAGGGTTTGGGGTTTCTCTCTTGCTCTGCGCGCCCGGCGCTCTGGCTCCT17400.11502945811869983No Hit
TCTCGGGTTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCC17360.11476502258279478No Hit
GCCGGGGTTTGCCCAGGGGTGTTACCAAACGTGGCAAAACAGCCTGTGATAA17050.11271564717953057No Hit
TTCCGGGTTTGGTGACGTCACCATTGCCGAAGTCCGCATGGAAACCCGCAGC17040.11264953829555431No Hit
AAAGGGGTTTGGGCATGTGCGTCCCATGGTGTGGCCCCAAGCTTCGGGCATG16750.11073238066024264No Hit
ACCTGGGTTTGGGCAATTCTCCTCCCTCAGCCTCCCAAGTAGCTAGGATTGC16570.10954242074866988No Hit
GGATGGGTTTGGGCAAGGTTCGCTGCAGCAGGTACCTTTACACCCTGGTTAT16430.1086168963730022No Hit
TACGGGGTTTGGGCTCTTCTGGGATCACAGGCCTGCACACTACAGCTGGTTT16400.10841856972107339No Hit
TTTGGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA16360.10815413418516834No Hit
ATGTGGGTTTGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGC16340.10802191641721581No Hit
ATGTGGGTTTGGGGTGGGTGATTGCATGAGCCCCGGGGTTCGAGACCAGCTT16110.10650141208576173No Hit
TTCAGGGTTTGGGTACGCTCTAAGCTCGGCAATCGCTCTTGCCGTGCGAGAT16050.10610475878190416No Hit
TCTCGGGTTTGGGAGAGTTTGAGGGTTTGGGTGGCTGGCCGCTGTATGAATC16000.10577421436202283No Hit
TGTGGGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15420.10193989909139951No Hit
TTAAGGGTTTGGGAGATTGGGACCATCCTGGCTAACACGGTGAAACCCCATC15220.10061772141187421No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCGA1450.046.00000421
GATACTC700.046.00000438
GCCTTTA2150.046.00000432
TTGGCCT351.0198528E-746.0000049
TCGCAGT351.0198528E-746.00000444
TACCCGT351.0198528E-746.00000439
CAAGTTA700.046.00000444
TTAATGT351.0198528E-746.00000420
CTACTCT700.046.00000423
CTTCCGG700.046.00000417
TTTACGA351.0198528E-746.00000415
TTAGCAG351.0198528E-746.00000428
GACGTTC700.046.00000415
CTGAGGT700.046.00000425
CATTGCT1450.046.00000433
CTATACT1400.046.00000440
TATGGAT351.0198528E-746.00000445
TAAACTG351.0198528E-746.00000423
TAAACGT1650.046.00000441
TAAACGC351.0198528E-746.00000423
Produced by FastQC (version 0.11.5)