FastQCFastQC Report
Fri 17 Jun 2016
SRR1528148_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528148_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1507395
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGTTTGGTGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAG39140.25965324284610203No Hit
ATAGGGGTTTGGGACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCA36750.24379807548784493No Hit
CCAGGGGTTTGGGGGGAGGCCGAGGTGGGCGGATCATGAAGTCAGGAGATCG28590.18966495178768672No Hit
TCTTGGGTTTAGGAGGCCTCTGTACCTTCCAAGGGGCTCCCCCCTCTGCTCT25640.17009476613628147No Hit
CATAGGGTTTGGGCGAGGTCAGGAGATCGAGGCCATCCCGGCTAAAACGGTG24910.1652519744327134No Hit
GATAGGGTTTGGGAACATTACAGGGCTTGCCTCAAATCTCTGAAAAGCCACA24720.16399152179753812No Hit
CTCAGGGTTTGGGCTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACC24150.16021016389201237No Hit
GATGGGGTTTCCCAGGACTGGGCTAGGGAGTGTTGGTCTAGATGCAAACCTA21160.14037461979109656No Hit
GGCAGGGTTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAG21070.13977756327969776No Hit
TATGGGGTTTGGGGTTGCTGGGTTTGGGAAAACCCTCACTAGTGACTGAAGA19260.12777009343934403No Hit
TCAAGGGTTTGGGGCCGGCGTTGTGGCGCACGCCTTTAATCCCAGCATTGGG18290.12133515103871248No Hit
GACTGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA17760.1178191515826973No Hit
GGATGGGTTTGGGCAAGGTTCGCTGCAGCAGGTACCTTTACACCCTGGTTAT17700.11742111390843142No Hit
CCAGGGGTTTGGGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCG17610.11682405739703262No Hit
TTCCGGGTTTGGTGACGTCACCATTGCCGAAGTCCGCATGGAAACCCGCAGC17350.11509922747521385No Hit
ATGTGGGTTTGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGC17250.11443583135143742No Hit
TCTTGGGTTTGGGTACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGG17200.1141041332895492No Hit
TCTCGGGTTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCC16840.11171590724395397No Hit
ACCTGGGTTTGGGCAATTCTCCTCCCTCAGCCTCCCAAGTAGCTAGGATTGC16790.11138420918206575No Hit
ACTAGGGTTTGGGGTTTCTCTCTTGCTCTGCGCGCCCGGCGCTCTGGCTCCT16720.11091983189542225No Hit
TTTGGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA16450.10912866236122583No Hit
GCCGGGGTTTGCCCAGGGGTGTTACCAAACGTGGCAAAACAGCCTGTGATAA16230.10766919088891765No Hit
TACGGGGTTTGGGCTCTTCTGGGATCACAGGCCTGCACACTACAGCTGGTTT16050.10647507786612004No Hit
TCTCGGGTTTGGGAGAGTTTGAGGGTTTGGGTGGCTGGCCGCTGTATGAATC15940.10574534212996593No Hit
TTAAGGGTTTGGGAGATTGGGACCATCCTGGCTAACACGGTGAAACCCCATC15620.10362247453388129No Hit
AAAGGGGTTTGGGCATGTGCGTCCCATGGTGTGGCCCCAAGCTTCGGGCATG15590.10342345569674836No Hit
ATGTGGGTTTGGGGTGGGTGATTGCATGAGCCCCGGGGTTCGAGACCAGCTT15540.10309175763486013No Hit
TCGAGGGTTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCC15520.10295907841010486No Hit
TGTGGGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT15180.10070353158926491No Hit
CATGGGGTTTGGGGAGTATTATGGGTTTGGGGGCTGGTGAGATGGCTCAGTG15080.10004013546548848No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATG405.6134013E-946.00000419
TATTTAG351.0198528E-746.00000433
CTGCTAC405.6134013E-946.00000443
GAAATCT206.312082E-446.00000413
GATACTC700.046.00000438
TGGAGAC206.312082E-446.00000420
CCTTAGT206.312082E-446.00000435
CTGTCCC206.312082E-446.00000432
TCCATCC206.312082E-446.00000429
ACAGTAC800.046.00000428
CAAACAT2150.046.00000445
CTACTTG351.0198528E-746.00000443
CTACTTA206.312082E-446.00000418
ATGATCT206.312082E-446.00000429
CATATAC351.0198528E-746.00000432
CCCTCAA700.046.00000444
CTCCGTA405.6134013E-946.00000431
CTGTAGG405.6134013E-946.00000419
AATCAAG351.0198528E-746.00000441
TACTTCA206.312082E-446.00000417
Produced by FastQC (version 0.11.5)