##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528148_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1507395
Sequences flagged as poor quality	0
Sequence length	52
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.476512791935757	31.0	31.0	33.0	28.0	33.0
2	31.26175023799336	31.0	31.0	33.0	30.0	34.0
3	31.748692280391005	31.0	31.0	34.0	30.0	34.0
4	35.532149834648514	37.0	35.0	37.0	33.0	37.0
5	35.75274961108402	37.0	35.0	37.0	35.0	37.0
6	35.52918445397523	37.0	35.0	37.0	33.0	37.0
7	35.39845694061609	37.0	35.0	37.0	32.0	37.0
8	31.44417488448615	33.0	31.0	35.0	25.0	35.0
9	35.91900464045589	37.0	35.0	39.0	32.0	39.0
10	37.31653216310257	39.0	37.0	39.0	34.0	39.0
11	37.33071358204054	39.0	37.0	39.0	35.0	39.0
12	37.4770720348681	39.0	37.0	39.0	35.0	39.0
13	37.38261106080357	39.0	37.0	39.0	35.0	39.0
14	37.51421027666935	40.0	37.0	40.0	32.0	40.0
15	36.81392070426133	39.0	36.0	40.0	31.0	40.0
16	36.67433817944202	38.0	35.0	40.0	31.0	40.0
17	36.611100607339154	39.0	36.0	40.0	31.0	40.0
18	36.62324606357325	39.0	35.0	40.0	31.0	40.0
19	36.87540293022068	39.0	36.0	40.0	31.0	40.0
20	37.074543168844265	39.0	36.0	40.0	32.0	40.0
21	37.17474782654845	39.0	36.0	40.0	32.0	40.0
22	36.96398820481692	39.0	36.0	40.0	31.0	40.0
23	37.01142500804368	39.0	36.0	40.0	31.0	40.0
24	37.093656937962514	39.0	36.0	40.0	32.0	40.0
25	36.94327764122874	39.0	35.0	40.0	31.0	40.0
26	36.533300826923266	39.0	35.0	40.0	30.0	40.0
27	36.16234497261833	38.0	35.0	40.0	30.0	40.0
28	35.99856441078815	38.0	35.0	40.0	30.0	40.0
29	36.01540936516308	38.0	35.0	40.0	30.0	40.0
30	35.79023348226576	38.0	35.0	40.0	30.0	40.0
31	35.746522975066256	38.0	35.0	40.0	30.0	40.0
32	35.7158409043416	38.0	35.0	40.0	30.0	40.0
33	35.64115775891521	38.0	35.0	40.0	30.0	40.0
34	35.48005997100959	38.0	35.0	40.0	29.0	40.0
35	35.16864590900195	37.0	34.0	40.0	27.0	40.0
36	34.95866312413136	37.0	34.0	40.0	27.0	40.0
37	34.7190776140295	36.0	34.0	40.0	26.0	40.0
38	34.287965662616635	35.0	33.0	40.0	24.0	40.0
39	33.87745813141214	35.0	33.0	39.0	23.0	40.0
40	33.39106073723211	35.0	33.0	39.0	21.0	40.0
41	32.90407358389805	35.0	33.0	39.0	19.0	40.0
42	32.41645089707741	35.0	32.0	39.0	16.0	40.0
43	31.9337313710076	35.0	31.0	39.0	14.0	40.0
44	31.375465621154376	35.0	31.0	38.0	10.0	40.0
45	31.10063918216526	35.0	30.0	38.0	10.0	40.0
46	30.746629118446062	35.0	29.0	38.0	9.0	40.0
47	30.356453351643065	35.0	27.0	37.0	9.0	40.0
48	29.94011324171833	35.0	25.0	37.0	9.0	40.0
49	29.420523485881272	35.0	24.0	37.0	8.0	40.0
50	29.023424517130547	34.0	23.0	36.0	8.0	40.0
51	28.564699365461607	34.0	22.0	36.0	8.0	39.0
52	27.535166296823327	33.0	20.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	7.0
14	6.0
15	23.0
16	77.0
17	246.0
18	587.0
19	1239.0
20	2503.0
21	4279.0
22	6717.0
23	10081.0
24	14692.0
25	20618.0
26	27769.0
27	36125.0
28	46413.0
29	58358.0
30	68349.0
31	72554.0
32	76409.0
33	88516.0
34	116020.0
35	151195.0
36	204747.0
37	248566.0
38	224337.0
39	26957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.083883122870912	21.694114681287918	40.63593152425211	8.586070671589066
2	15.977364924256749	24.734591795780137	35.37194962169836	23.916093658264757
3	16.60427426122549	25.85831848984506	38.872889985703814	18.66451726322563
4	19.68966329329738	24.65936267534389	34.19627901114174	21.454695020216995
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	98.81318433456393	0.4136274831746158	0.5715821002457883	0.2016060820156628
12	98.68912925941774	0.6865486484962469	0.15264744808096087	0.47167464400505504
13	94.79811197463172	2.5896331087737456	1.304303118956876	1.3079517976376465
14	44.14324049104581	31.72426603511356	13.810779523615244	10.321713950225389
15	28.538438829901917	39.1453467737388	21.55208157118738	10.764132825171902
16	32.13703110332726	33.54840635666166	19.7171942324341	14.59736830757698
17	23.36998596917198	32.56167096215657	22.762049761343246	21.306293307328204
18	18.613767459756733	38.763495964893075	24.203941236371357	18.418795338978835
19	22.14807664878814	36.77443536697415	27.72989163424318	13.347596349994529
20	20.424772538054057	37.15024927109351	25.28925729486963	17.135720895982807
21	27.54891717167697	35.64759071112748	16.52805004660358	20.275442070591982
22	27.83331508993993	37.22932608904766	20.383243940705654	14.554114880306754
23	22.627446687829003	37.62895591401059	24.492783908663622	15.250813489496782
24	25.230812096364918	38.55187260140839	18.771921095665036	17.44539420656165
25	28.347579765091435	36.67485960879531	20.71660049290332	14.26096013320994
26	22.7227103712033	38.24458751687514	21.617890466666005	17.414811645255558
27	21.914826571668343	40.85989405563903	22.082864809820915	15.142414562871709
28	20.820156627824822	41.10030881089562	19.305755956467944	18.77377860481161
29	23.401099247377097	43.963526481114776	16.732973109238124	15.902401162270008
30	24.6296425289987	46.13502101307222	15.703913042036097	13.531423415892982
31	20.326589911735145	49.43727423800663	16.33725732140547	13.898878528852757
32	20.676133329352954	50.61135269786619	17.343363882724834	11.369150090056024
33	21.182967967918167	50.00288577313843	15.867307507322234	12.946838751621174
34	18.68621031647312	52.221614109108764	15.933978817761767	13.158196756656352
35	19.89571412934234	51.617790957247436	15.423031123229148	13.063463790181073
36	19.49489019135661	51.38248435214393	17.453752997721235	11.668872458778223
37	19.194504426510637	51.983123202611125	17.513657667698247	11.308714703179989
38	16.342431811170925	53.54031292395158	18.724156574753135	11.393098690124354
39	16.86910199383705	54.268655528245745	17.998998271853097	10.863244206064104
40	20.20193778007755	51.90789408217488	16.07952792731832	11.81064021042925
41	22.486541351138886	51.3297443603037	15.261427827477203	10.922286461080208
42	20.980764829391102	50.67994785706467	14.647919092208744	13.691368221335482
43	19.287512563064094	49.23016196816362	17.8592207085734	13.623104760198887
44	18.11044882064754	49.00673015367571	19.172612354426015	13.710208671250735
45	18.508619174138165	48.59124516135452	20.44646559130155	12.453670073205762
46	22.09208601594141	47.93302352734353	17.12205493583301	12.852835520882053
47	22.109865032058618	47.392488365690475	16.348402376284916	14.149244225965987
48	22.86945359378265	48.51402585254694	14.431519276632867	14.185001277037538
49	21.0603060246319	49.00931739855844	15.463365607554755	14.467010969254906
50	19.047230487032266	49.166807638342966	16.465956169418103	15.320005705206666
51	18.445795561216535	49.3367697252545	16.207099001920533	16.010335711608437
52	21.513936294070234	46.72166220532773	16.11926535513253	15.645136145469502
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	3.5
6	7.0
7	18.0
8	29.0
9	826.5
10	1624.0
11	4881.5
12	8139.0
13	12450.5
14	19136.0
15	21510.0
16	21273.0
17	21036.0
18	18509.5
19	15983.0
20	16529.5
21	17076.0
22	21967.0
23	26858.0
24	37546.5
25	48235.0
26	59567.5
27	70900.0
28	78926.0
29	86952.0
30	85080.0
31	83208.0
32	74632.0
33	66056.0
34	61633.5
35	57211.0
36	56877.5
37	56544.0
38	53490.0
39	49869.0
40	49302.0
41	54370.5
42	59439.0
43	55781.0
44	52123.0
45	58674.5
46	65226.0
47	71050.5
48	76875.0
49	82696.5
50	88518.0
51	92818.0
52	97118.0
53	97149.5
54	97181.0
55	89439.0
56	81697.0
57	64022.0
58	46347.0
59	48790.5
60	51234.0
61	43608.0
62	35982.0
63	29708.5
64	18821.0
65	14207.0
66	11996.5
67	9786.0
68	8335.5
69	6885.0
70	4825.5
71	2766.0
72	1554.5
73	343.0
74	258.5
75	174.0
76	120.5
77	67.0
78	58.5
79	50.0
80	41.0
81	32.0
82	28.0
83	24.0
84	13.5
85	3.0
86	6.5
87	10.0
88	7.5
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1507395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.06917056742451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.51341584243092	29.1337115013888
2	6.555887941632703	4.4670732900881225
3	2.0983045427919085	2.1446248611233765
4	1.0409618376164809	1.4185882559974214
5	0.6456745917785721	1.0998798899178182
6	0.43524743466248816	0.8897111454330158
7	0.3218681046994918	0.7676045551454445
8	0.2657638869314873	0.7243484155624449
9	0.2155997103128975	0.6610772974443675
>10	2.326813806335602	17.729139152881718
>50	0.3128393593789234	7.259981867166244
>100	0.20723015405427467	14.869368183569835
>500	0.04361701310363027	10.299360563233527
>1k	0.016775774270627025	8.535531021047897
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTGGGGTTTGGTGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAG	3914	0.25965324284610203	No Hit
ATAGGGGTTTGGGACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCA	3675	0.24379807548784493	No Hit
CCAGGGGTTTGGGGGGAGGCCGAGGTGGGCGGATCATGAAGTCAGGAGATCG	2859	0.18966495178768672	No Hit
TCTTGGGTTTAGGAGGCCTCTGTACCTTCCAAGGGGCTCCCCCCTCTGCTCT	2564	0.17009476613628147	No Hit
CATAGGGTTTGGGCGAGGTCAGGAGATCGAGGCCATCCCGGCTAAAACGGTG	2491	0.1652519744327134	No Hit
GATAGGGTTTGGGAACATTACAGGGCTTGCCTCAAATCTCTGAAAAGCCACA	2472	0.16399152179753812	No Hit
CTCAGGGTTTGGGCTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACC	2415	0.16021016389201237	No Hit
GATGGGGTTTCCCAGGACTGGGCTAGGGAGTGTTGGTCTAGATGCAAACCTA	2116	0.14037461979109656	No Hit
GGCAGGGTTTGGGGCCGGTACTGGTGGCGCAGGCCGGTAGGATTTGCTGAAG	2107	0.13977756327969776	No Hit
TATGGGGTTTGGGGTTGCTGGGTTTGGGAAAACCCTCACTAGTGACTGAAGA	1926	0.12777009343934403	No Hit
TCAAGGGTTTGGGGCCGGCGTTGTGGCGCACGCCTTTAATCCCAGCATTGGG	1829	0.12133515103871248	No Hit
GACTGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA	1776	0.1178191515826973	No Hit
GGATGGGTTTGGGCAAGGTTCGCTGCAGCAGGTACCTTTACACCCTGGTTAT	1770	0.11742111390843142	No Hit
CCAGGGGTTTGGGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCG	1761	0.11682405739703262	No Hit
TTCCGGGTTTGGTGACGTCACCATTGCCGAAGTCCGCATGGAAACCCGCAGC	1735	0.11509922747521385	No Hit
ATGTGGGTTTGGGGCTTTCTGCGCTCTCGCTGGACAAGTGAGCAGGGACGGC	1725	0.11443583135143742	No Hit
TCTTGGGTTTGGGTACAAAGATGGCGGCTGCCGCTGCTAGCCGAGCGGTCGG	1720	0.1141041332895492	No Hit
TCTCGGGTTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCC	1684	0.11171590724395397	No Hit
ACCTGGGTTTGGGCAATTCTCCTCCCTCAGCCTCCCAAGTAGCTAGGATTGC	1679	0.11138420918206575	No Hit
ACTAGGGTTTGGGGTTTCTCTCTTGCTCTGCGCGCCCGGCGCTCTGGCTCCT	1672	0.11091983189542225	No Hit
TTTGGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTA	1645	0.10912866236122583	No Hit
GCCGGGGTTTGCCCAGGGGTGTTACCAAACGTGGCAAAACAGCCTGTGATAA	1623	0.10766919088891765	No Hit
TACGGGGTTTGGGCTCTTCTGGGATCACAGGCCTGCACACTACAGCTGGTTT	1605	0.10647507786612004	No Hit
TCTCGGGTTTGGGAGAGTTTGAGGGTTTGGGTGGCTGGCCGCTGTATGAATC	1594	0.10574534212996593	No Hit
TTAAGGGTTTGGGAGATTGGGACCATCCTGGCTAACACGGTGAAACCCCATC	1562	0.10362247453388129	No Hit
AAAGGGGTTTGGGCATGTGCGTCCCATGGTGTGGCCCCAAGCTTCGGGCATG	1559	0.10342345569674836	No Hit
ATGTGGGTTTGGGGTGGGTGATTGCATGAGCCCCGGGGTTCGAGACCAGCTT	1554	0.10309175763486013	No Hit
TCGAGGGTTTGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCCTCCC	1552	0.10295907841010486	No Hit
TGTGGGGTTTGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACT	1518	0.10070353158926491	No Hit
CATGGGGTTTGGGGAGTATTATGGGTTTGGGGGCTGGTGAGATGGCTCAGTG	1508	0.10004013546548848	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.011609432166087853	0.0	0.0	0.0	0.0
12	0.033766862700221244	0.0	0.0	0.0	0.0
13	0.09393689112674515	0.0	0.0	0.0	0.0
14	0.5506187827344525	0.0	0.0	0.0	0.0
15	1.0068362970555162	0.0	0.0	0.0	0.0
16	1.2451281847160167	0.0	0.0	0.0	0.0
17	1.3857018233442462	0.0	0.0	0.0	0.0
18	1.540604818246047	0.0	0.0	0.0	0.0
19	1.6995545295028842	0.0	0.0	0.0	0.0
20	1.8725018989714044	0.0	0.0	0.0	0.0
21	2.0475057964236316	0.0	0.0	0.0	0.0
22	2.2241018445729224	0.0	0.0	0.0	0.0
23	2.3729015951359798	0.0	0.0	0.0	0.0
24	2.5447875308064574	0.0	0.0	0.0	0.0
25	2.735248557942676	0.0	0.0	0.0	0.0
26	2.9635895037465296	0.0	0.0	0.0	0.0
27	3.2209871997717916	0.0	0.0	0.0	0.0
28	3.4707558403736245	0.0	0.0	0.0	0.0
29	3.788124545988278	0.0	0.0	0.0	0.0
30	4.161218526000153	0.0	0.0	0.0	0.0
31	4.463196441543192	0.0	0.0	0.0	0.0
32	4.681785464327532	0.0	0.0	0.0	0.0
33	4.878150716965361	0.0	0.0	0.0	0.0
34	5.081614308127597	0.0	0.0	0.0	0.0
35	5.260266884260595	0.0	0.0	0.0	0.0
36	5.442966176748629	0.0	0.0	0.0	0.0
37	5.632830147373449	0.0	0.0	0.0	0.0
38	5.833242116366314	0.0	0.0	0.0	0.0
39	6.031929255437361	0.0	0.0	0.0	0.0
40	6.233734356290157	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAATG	40	5.6134013E-9	46.000004	19
TATTTAG	35	1.0198528E-7	46.000004	33
CTGCTAC	40	5.6134013E-9	46.000004	43
GAAATCT	20	6.312082E-4	46.000004	13
GATACTC	70	0.0	46.000004	38
TGGAGAC	20	6.312082E-4	46.000004	20
CCTTAGT	20	6.312082E-4	46.000004	35
CTGTCCC	20	6.312082E-4	46.000004	32
TCCATCC	20	6.312082E-4	46.000004	29
ACAGTAC	80	0.0	46.000004	28
CAAACAT	215	0.0	46.000004	45
CTACTTG	35	1.0198528E-7	46.000004	43
CTACTTA	20	6.312082E-4	46.000004	18
ATGATCT	20	6.312082E-4	46.000004	29
CATATAC	35	1.0198528E-7	46.000004	32
CCCTCAA	70	0.0	46.000004	44
CTCCGTA	40	5.6134013E-9	46.000004	31
CTGTAGG	40	5.6134013E-9	46.000004	19
AATCAAG	35	1.0198528E-7	46.000004	41
TACTTCA	20	6.312082E-4	46.000004	17
>>END_MODULE