FastQCFastQC Report
Fri 17 Jun 2016
SRR1528147_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528147_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1324147
Sequences flagged as poor quality0
Sequence length59
%GC41

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAGGGGTTTGGGACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCATGCGCCG34520.2606961311697266No Hit
CTTGGGGTTTGGTGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAGATAAAAT29070.21953755889640653No Hit
CATAGGGTTTGGGCGAGGTCAGGAGATCGAGGCCATCCCGGCTAAAACGGTGAAACCCC22970.17347016607672713No Hit
CCAGGGGTTTGGGGGGAGGCCGAGGTGGGCGGATCATGAAGTCAGGAGATCGAGACCTT22310.16848582521427002No Hit
GATAGGGTTTGGGAACATTACAGGGCTTGCCTCAAATCTCTGAAAAGCCACATGTAAGC21100.159347866966432No Hit
TCTTGGGTTTAGGAGGCCTCTGTACCTTCCAAGGGGCTCCCCCCTCTGCTCTGCACCAG20850.1574598590639861No Hit
GATGGGGTTTCCCAGGACTGGGCTAGGGAGTGTTGGTCTAGATGCAAACCTACAGAGTT18800.1419781942639299No Hit
CTCAGGGTTTGGGCTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACCAAGAAAG17930.13540792676341826No Hit
GACTGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTACTCTTCT17740.1339730407575594No Hit
TATGGGGTTTGGGGTTGCTGGGTTTGGGAAAACCCTCACTAGTGACTGAAGATGAAAAG16010.12090802607263393No Hit
AAAGGGGTTTGGGCATGTGCGTCCCATGGTGTGGCCCCAAGCTTCGGGCATGGCAGTAC15430.1165278477389595No Hit
TCAAGGGTTTGGGGCCGGCGTTGTGGCGCACGCCTTTAATCCCAGCATTGGGGAGGCAG15280.11539504299749198No Hit
TTTGGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTACTCTTCT14800.11177006782479587No Hit
CCAGGGGTTTGGGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGC14620.11041070213503486No Hit
ACCTGGGTTTGGGCAATTCTCCTCCCTCAGCCTCCCAAGTAGCTAGGATTGCAGGTGCC14030.10595500348526259No Hit
ACTAGGGTTTGGGGTTTCTCTCTTGCTCTGCGCGCCCGGCGCTCTGGCTCCTAAAGATG13470.10172586578378383No Hit
GGATGGGTTTGGGCAAGGTTCGCTGCAGCAGGTACCTTTACACCCTGGTTATCACAGAC13450.10157482515158817No Hit
TACGGGGTTTGGGCTCTTCTGGGATCACAGGCCTGCACACTACAGCTGGTTTGTGCAGT13270.10021545946182711No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTCAC650.053.00000428
CTAGATG2450.053.00000438
GACAGTT750.053.00000450
TTAATGT650.053.00000420
CTTCACT650.053.00000429
GACGTTC650.053.00000415
CACATCG650.053.00000437
ACACATC650.053.00000436
ACATTAC2450.053.00000415
ATTGTAC750.053.00000431
TATATGA650.053.00000429
TTACTGT750.053.00000444
CCTAATA650.053.00000435
CCTAAAT650.053.00000431
TCTAGCT750.053.00000437
CATTACA2450.053.00000416
TATGAAG650.053.00000431
CACCTAG650.053.00000418
TACTGTA750.053.00000445
GCTTAAG1500.053.00000437