##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528147_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1324147
Sequences flagged as poor quality	0
Sequence length	59
%GC	41
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.12989871970408	31.0	30.0	31.0	27.0	33.0
2	30.883821056121413	31.0	31.0	33.0	28.0	34.0
3	31.427923787917805	31.0	31.0	34.0	30.0	34.0
4	35.29061728040769	35.0	35.0	37.0	33.0	37.0
5	35.37799428613288	37.0	35.0	37.0	33.0	37.0
6	35.239129794501665	37.0	35.0	37.0	32.0	37.0
7	34.9453013902535	37.0	35.0	37.0	32.0	37.0
8	30.28819685427675	32.0	28.0	35.0	17.0	35.0
9	35.21602661940102	37.0	34.0	37.0	31.0	39.0
10	36.878548982854625	37.0	37.0	39.0	34.0	39.0
11	36.60061156351976	37.0	35.0	39.0	33.0	39.0
12	36.95869642871977	39.0	37.0	39.0	34.0	39.0
13	36.90626720447201	39.0	37.0	39.0	32.0	39.0
14	36.62108436601072	39.0	36.0	40.0	30.0	40.0
15	35.836674477984694	38.0	34.0	40.0	29.0	40.0
16	35.55198554239069	38.0	34.0	40.0	29.0	40.0
17	35.67026244065047	38.0	34.0	40.0	28.0	40.0
18	35.859907548029035	38.0	34.0	40.0	29.0	40.0
19	36.208402843490944	38.0	35.0	40.0	30.0	40.0
20	36.30283797795864	38.0	35.0	40.0	30.0	40.0
21	36.31114068151044	38.0	35.0	40.0	30.0	40.0
22	36.10409342769345	38.0	35.0	40.0	30.0	40.0
23	36.042860044995	38.0	35.0	40.0	30.0	40.0
24	36.097464254346384	38.0	35.0	40.0	30.0	40.0
25	35.92090983856022	38.0	34.0	40.0	30.0	40.0
26	35.41043403791271	38.0	34.0	40.0	27.0	40.0
27	35.04706577139849	38.0	34.0	40.0	26.0	40.0
28	34.8214586446973	37.0	33.0	40.0	26.0	40.0
29	34.77041748385942	37.0	33.0	40.0	26.0	40.0
30	34.58722558749142	37.0	33.0	40.0	25.0	40.0
31	34.495564314233995	37.0	33.0	40.0	25.0	40.0
32	34.38459778257248	36.0	33.0	40.0	25.0	40.0
33	34.08083845675745	36.0	33.0	40.0	23.0	40.0
34	33.847681564055954	35.0	33.0	40.0	23.0	40.0
35	33.74157703034482	35.0	33.0	39.0	23.0	40.0
36	33.32921042754317	35.0	32.0	39.0	21.0	40.0
37	33.247409841958635	35.0	32.0	39.0	21.0	40.0
38	32.86527024567514	35.0	32.0	39.0	21.0	40.0
39	32.419141530358786	35.0	31.0	38.0	19.0	40.0
40	32.024640013533244	35.0	31.0	38.0	18.0	40.0
41	31.545546680240186	35.0	30.0	38.0	15.0	40.0
42	31.110733929087935	35.0	29.0	38.0	13.0	40.0
43	30.6219943858197	35.0	28.0	37.0	10.0	40.0
44	30.15107235072843	34.0	26.0	37.0	10.0	40.0
45	29.948770793575033	34.0	25.0	37.0	9.0	40.0
46	29.589191381319445	34.0	24.0	37.0	9.0	40.0
47	29.070891675924198	33.0	23.0	36.0	9.0	40.0
48	28.664801566593436	33.0	23.0	36.0	8.0	39.0
49	28.23591867066119	33.0	21.0	35.0	8.0	39.0
50	27.645887503426735	33.0	20.0	35.0	8.0	39.0
51	27.19541108351263	33.0	20.0	35.0	8.0	39.0
52	26.469406342347188	33.0	15.0	35.0	8.0	38.0
53	26.146675557925214	32.0	15.0	35.0	8.0	38.0
54	25.738541868840848	31.0	12.0	35.0	8.0	38.0
55	25.316441452497344	31.0	10.0	35.0	8.0	37.0
56	24.758819073713116	31.0	10.0	35.0	8.0	37.0
57	24.239627473384754	30.0	10.0	35.0	8.0	37.0
58	23.78415613976394	29.0	10.0	35.0	8.0	36.0
59	23.09095591350507	27.0	9.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	5.0
12	3.0
13	8.0
14	25.0
15	121.0
16	355.0
17	1035.0
18	2271.0
19	4494.0
20	7618.0
21	12264.0
22	17746.0
23	24357.0
24	32214.0
25	41636.0
26	51383.0
27	60723.0
28	64198.0
29	63784.0
30	68826.0
31	78659.0
32	90923.0
33	103503.0
34	119204.0
35	128731.0
36	140820.0
37	121814.0
38	83387.0
39	4039.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.35958016745875	22.405820501802292	39.55444523908599	8.680154091652966
2	14.94645231987083	25.461145930172407	35.09761378457226	24.494787965384507
3	16.458746649729978	26.29360637451884	38.635136431227046	18.612510544524135
4	19.9008871371532	24.872616106821976	34.05936047885922	21.167136277165604
5	100.0	0.0	0.0	0.0
6	100.0	0.0	0.0	0.0
7	100.0	0.0	0.0	0.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	98.7512715733223	0.4325803706083992	0.5904933515689723	0.22565470450033118
12	98.65415244682049	0.6882166405995709	0.15964994823082332	0.49798096434912437
13	94.79000443304255	2.6021280114670047	1.2633793680006828	1.3444881874897574
14	44.01746935951975	31.666952385195902	13.835926071652166	10.47965218363218
15	28.63737938461515	38.78496873836515	21.69321079910312	10.884441077916577
16	32.126795589915616	33.09760925335329	20.030631040209283	14.744964116521805
17	23.431688475675283	32.11916803798974	22.739544778638628	21.70959870769635
18	18.537820951903377	38.600548126454235	24.265055163814893	18.59657575782749
19	22.189077194601506	36.40555013907066	27.949087223699483	13.45628544262835
20	20.31013173008737	36.7042329892376	25.64126188406574	17.34437339660929
21	27.58794907211964	35.28716977797782	16.78658034191068	20.338300807991864
22	28.03646422942468	36.70128769690979	20.488057594813867	14.774190478851668
23	22.42477610114285	37.25432297169423	24.910678346135285	15.410222581027636
24	24.971396680277948	38.527822062052024	18.888839381126115	17.61194187654392
25	28.391258674452306	36.59125459635524	20.697475431353165	14.320011297839288
26	22.535866486122764	38.25338123335249	21.756874425573596	17.45387785495115
27	21.916222292540027	40.7891268869695	22.048987008240022	15.24566381225045
28	20.744826669546505	41.097023215700375	19.18616286560329	18.97198724914983
29	23.33449382885737	43.911212274770094	16.733414039377802	16.02087985699473
30	24.55324069004423	46.18437378931493	15.738509395104924	13.52387612553591
31	20.113174745704214	49.39376066252463	16.43737440027429	14.055690191496867
32	20.638116462900268	50.82826906680301	17.176869335504293	11.356745134792435
33	21.13647502882988	50.190802078621175	15.828000969680858	12.84472192286808
34	18.68251787754683	52.32281612237916	16.014007508229827	12.980658491844183
35	20.006086937477484	51.88034259036195	15.213643198224972	12.8999272739356
36	19.359784072312213	51.459694429697	17.595402927318492	11.58511857067229
37	19.09576504723418	52.19337430058747	17.483406298545404	11.227454353632943
38	15.975869748600418	54.117480914128116	18.616664161909515	11.289985175361949
39	16.599818600200734	54.82782500734436	17.882531169122462	10.689825223332456
40	19.912970387728855	52.816416908394615	15.648715739264599	11.621896964611935
41	22.201538046757648	52.10297648221837	15.06924835384591	10.626237117178079
42	20.66590793922427	51.571540017837904	14.269186125105445	13.493365917832387
43	18.74316069137339	50.17962507183869	17.716235433075028	13.36097880371288
44	17.503268141679136	49.888947375178134	18.987846515530375	13.619937967612358
45	17.73164157755899	49.57765263222286	20.236046299995394	12.454659490222761
46	21.391733697240564	48.85190239452266	16.89351710950521	12.862846798731562
47	21.614518629729176	48.04632718270705	16.151756564792276	14.187397622771488
48	22.26180325900372	49.2726260755037	14.316461842982688	14.149108822509888
49	20.766274439318295	49.6181315216513	15.200880264804436	14.414713774225973
50	18.285129974240018	49.9947513380312	16.28603168681423	15.43408700091455
51	17.751956542589305	49.8308722520989	16.329606909202678	16.087564296109118
52	19.424656023840253	48.425892291414776	16.326586096558763	15.822865588186206
53	19.711708745328124	49.23826433167919	15.627947652337692	15.422079270654995
54	19.605602701210668	48.327489319539296	16.283841597647392	15.783066381602648
55	19.68535215500998	46.86549152020131	17.88502333955369	15.564132985235023
56	19.45901776766477	45.027780148276584	18.33089528579531	17.182306798263337
57	21.16857116317146	43.323513174896746	18.08424593341978	17.423669728512014
58	19.9498243019846	43.1786652086211	18.96013055952247	17.911379929871835
59	22.8240520123521	39.937786363598605	19.593670491267208	17.644491132782086
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	4.0
5	8.0
6	15.0
7	22.0
8	611.5
9	3521.5
10	5842.0
11	8988.5
12	12135.0
13	13836.0
14	15028.5
15	14520.0
16	12224.0
17	9928.0
18	9263.5
19	9624.0
20	10649.0
21	13232.5
22	15816.0
23	19187.5
24	22559.0
25	30262.0
26	42828.0
27	47691.0
28	52897.5
29	58104.0
30	61556.5
31	65464.5
32	65920.0
33	65816.5
34	65713.0
35	61089.5
36	55223.0
37	53980.0
38	52305.0
39	50630.0
40	48319.5
41	46009.0
42	48698.0
43	50374.0
44	49361.0
45	50181.5
46	51002.0
47	55166.0
48	63725.0
49	68120.0
50	64291.5
51	60463.0
52	62787.0
53	62194.0
54	59277.0
55	51781.0
56	44285.0
57	38061.0
58	33425.5
59	35014.0
60	33624.5
61	32235.0
62	29588.0
63	26941.0
64	18223.0
65	8829.5
66	8154.0
67	6222.5
68	4291.0
69	3186.0
70	1219.0
71	357.0
72	351.0
73	345.0
74	408.5
75	296.5
76	121.0
77	95.5
78	70.0
79	48.0
80	26.0
81	27.5
82	21.0
83	13.0
84	9.0
85	5.0
86	8.0
87	6.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1324147.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.816629860411076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.49303757995847	46.493678255054306
2	4.156892742018767	4.22478559681194
3	1.2854868150093144	1.9597232300630116
4	0.6150716901441523	1.2502348166269135
5	0.3786401523487691	0.9620608236098529
6	0.2423134750234066	0.7388132502272651
7	0.18845058886290958	0.6703496674856088
8	0.1456369154079208	0.5920621779437047
9	0.11678738974908685	0.5341267401518621
>10	1.1253652424532654	12.19060935549623
>50	0.11259700849553371	3.9124445734524387
>100	0.10937938139926526	12.747914201856211
>500	0.021238713390438903	7.415963034781378
>1k	0.00910230573875953	6.307234276439373
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATAGGGGTTTGGGACAAGCCCCTAGACCTGCGACCCAAGAAGACTAGAGCCATGCGCCG	3452	0.2606961311697266	No Hit
CTTGGGGTTTGGTGTGCAGCGTTGCTCCCCGTTAACTCGCGTAAAGGCGCAGATAAAAT	2907	0.21953755889640653	No Hit
CATAGGGTTTGGGCGAGGTCAGGAGATCGAGGCCATCCCGGCTAAAACGGTGAAACCCC	2297	0.17347016607672713	No Hit
CCAGGGGTTTGGGGGGAGGCCGAGGTGGGCGGATCATGAAGTCAGGAGATCGAGACCTT	2231	0.16848582521427002	No Hit
GATAGGGTTTGGGAACATTACAGGGCTTGCCTCAAATCTCTGAAAAGCCACATGTAAGC	2110	0.159347866966432	No Hit
TCTTGGGTTTAGGAGGCCTCTGTACCTTCCAAGGGGCTCCCCCCTCTGCTCTGCACCAG	2085	0.1574598590639861	No Hit
GATGGGGTTTCCCAGGACTGGGCTAGGGAGTGTTGGTCTAGATGCAAACCTACAGAGTT	1880	0.1419781942639299	No Hit
CTCAGGGTTTGGGCTGGACGCTTTGCGTGAAAATCTGCATTTGATCGGTACCAAGAAAG	1793	0.13540792676341826	No Hit
GACTGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTACTCTTCT	1774	0.1339730407575594	No Hit
TATGGGGTTTGGGGTTGCTGGGTTTGGGAAAACCCTCACTAGTGACTGAAGATGAAAAG	1601	0.12090802607263393	No Hit
AAAGGGGTTTGGGCATGTGCGTCCCATGGTGTGGCCCCAAGCTTCGGGCATGGCAGTAC	1543	0.1165278477389595	No Hit
TCAAGGGTTTGGGGCCGGCGTTGTGGCGCACGCCTTTAATCCCAGCATTGGGGAGGCAG	1528	0.11539504299749198	No Hit
TTTGGGGTTTGGGGGGCTGGAGAGATGGCTCAGCAGTTAAGAGCACTGACTACTCTTCT	1480	0.11177006782479587	No Hit
CCAGGGGTTTGGGAAATAAAACGGGATAGCTGAATAAGTACCACGGTGAGCGTAAACGC	1462	0.11041070213503486	No Hit
ACCTGGGTTTGGGCAATTCTCCTCCCTCAGCCTCCCAAGTAGCTAGGATTGCAGGTGCC	1403	0.10595500348526259	No Hit
ACTAGGGTTTGGGGTTTCTCTCTTGCTCTGCGCGCCCGGCGCTCTGGCTCCTAAAGATG	1347	0.10172586578378383	No Hit
GGATGGGTTTGGGCAAGGTTCGCTGCAGCAGGTACCTTTACACCCTGGTTATCACAGAC	1345	0.10157482515158817	No Hit
TACGGGGTTTGGGCTCTTCTGGGATCACAGGCCTGCACACTACAGCTGGTTTGTGCAGT	1327	0.10021545946182711	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.009515559828327217	0.0	0.0	0.0	0.0
12	0.028999801381568664	0.0	0.0	0.0	0.0
13	0.07763488494857444	0.0	0.0	0.0	0.0
14	0.47555143046806736	0.0	0.0	0.0	0.0
15	0.8633482536304504	0.0	0.0	0.0	0.0
16	1.0745030574399972	0.0	0.0	0.0	0.0
17	1.1976766929955662	0.0	0.0	0.0	0.0
18	1.3338398229199628	0.0	0.0	0.0	0.0
19	1.4726461639077837	0.0	0.0	0.0	0.0
20	1.6233847148390623	0.0	0.0	0.0	0.0
21	1.770573810913743	0.0	0.0	0.0	0.0
22	1.9147420943445101	0.0	0.0	0.0	0.0
23	2.0491682569986565	0.0	0.0	0.0	0.0
24	2.1961307921250435	0.0	0.0	0.0	0.0
25	2.3655228611324874	0.0	0.0	0.0	0.0
26	2.558930390659043	0.0	0.0	0.0	0.0
27	2.764647731709546	0.0	0.0	0.0	0.0
28	2.976557738680071	0.0	0.0	0.0	0.0
29	3.2350637806829603	0.0	0.0	0.0	0.0
30	3.5488506940694653	0.0	0.0	0.0	0.0
31	3.803052078054778	0.0	0.0	0.0	0.0
32	3.9960064856847466	0.0	0.0	0.0	0.0
33	4.1638881483702335	0.0	0.0	0.0	0.0
34	4.324897462290818	0.0	0.0	0.0	0.0
35	4.476466736699173	0.0	0.0	0.0	0.0
36	4.636041164613899	0.0	0.0	0.0	0.0
37	4.794482787787157	0.0	0.0	0.0	0.0
38	4.967499831967297	0.0	0.0	0.0	0.0
39	5.1336445273825335	0.0	0.0	0.0	0.0
40	5.302054832280706	0.0	0.0	0.0	0.0
41	5.4732593888744985	0.0	0.0	0.0	0.0
42	5.640083767134616	0.0	0.0	0.0	0.0
43	5.810382079935234	0.0	0.0	0.0	0.0
44	5.962706557504567	0.0	0.0	0.0	0.0
45	6.119562254039771	0.0	0.0	0.0	0.0
46	6.274152341092039	0.0	0.0	0.0	0.0
47	6.4085785037461855	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTTCAC	65	0.0	53.000004	28
CTAGATG	245	0.0	53.000004	38
GACAGTT	75	0.0	53.000004	50
TTAATGT	65	0.0	53.000004	20
CTTCACT	65	0.0	53.000004	29
GACGTTC	65	0.0	53.000004	15
CACATCG	65	0.0	53.000004	37
ACACATC	65	0.0	53.000004	36
ACATTAC	245	0.0	53.000004	15
ATTGTAC	75	0.0	53.000004	31
TATATGA	65	0.0	53.000004	29
TTACTGT	75	0.0	53.000004	44
CCTAATA	65	0.0	53.000004	35
CCTAAAT	65	0.0	53.000004	31
TCTAGCT	75	0.0	53.000004	37
CATTACA	245	0.0	53.000004	16
TATGAAG	65	0.0	53.000004	31
CACCTAG	65	0.0	53.000004	18
TACTGTA	75	0.0	53.000004	45
GCTTAAG	150	0.0	53.000004	37
>>END_MODULE