FastQCFastQC Report
Fri 17 Jun 2016
SRR1528146_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528146_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228167
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGAAGCGGGGGTACCGGTTTTAATGGCGATACGCTAGCCTTTAATCGC23161.0150459970109613No Hit
CGAATGAAGCGGGGGGTTCTTATCTGCCTAGTACCTTTCGAGCCCCGCGCGT16280.7135124711286032No Hit
GAATTGAAGCGGGGGAAGGCCGAAGTGGGCGGATCACAAGGTCAGGATTTCG16040.7029938597606139No Hit
AGCTTGAAGCGGGGATAGCTTCGTGCTCTAAAGATGGCCTTGATAACTGATA15640.6854628408139652No Hit
TATCTGAAGCGGGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTAT14020.6144622140800379No Hit
TTGATGAAGCAGGGAGGGTATTGGCCTAGTGTGTGAGAAGTTGATAAAGGAG12280.5382022816621159No Hit
TTGTTGAAGCGGGAATTAAATTAGCTGGACATGGTGGCACATGCTTGTGGTC11530.5053316211371496No Hit
GTTTTGAAGCGGGAGGCTGATCCTGCCCAAGAGTCCATATCGACGGCATGGT10990.4816647455591737No Hit
TACATGAAGCGGGGCCTCACATAGCCTTGGGAAAGTTGAGTCAATTGTACAT10610.46501027755985747No Hit
TGTGTGAAGCGGGGGGGGGTAGAGGTCGCAGTGAACTGAGATCATGCGCCAC9740.4268803113508965No Hit
TGCGTGAAGCGGGAACTTATTCTCTTAAGCTCACTTAAAAATGCATCGTTTA9380.4111023942989126No Hit
ATGCTGAAGCGGGGAGGGAATCCCTAAAGTGGAATAAAAATGAATCCAACTA9000.39444792629959635No Hit
TATTTGAAGCGGGCGTTCGCGTCCTTCACCCCCGGCAGCGCAAAAAAAATAA8960.39269482440493153No Hit
GCTGTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA8510.3729724280899517No Hit
AACTTGAAGCGGGAGAGGTTCCCTGCTGAGCTGTGTCTAGATTCCTAACTCA8490.3720958771426192No Hit
GCGATGAAGCGGGAGGGCGGATCACGAGGTCAGGAGATAGAGACCATCCTGG8450.37034277524795434No Hit
GCACTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT7980.3497438279856421No Hit
TTATTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6870.3010952504086919No Hit
GGGGTGAAGCGGGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAA6860.30065697493502563No Hit
ATATTGAAGCGGGATCCTCTAATTACTCCTTTTGTAGACTTAGAATGGTTAT6820.29890387304036076No Hit
AGTTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC6750.29583594472469726No Hit
CAGTTGAAGCGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG6530.28619388430404047No Hit
TGTTTGAAGCGGTCAGCTATTGACAAATATCTATTGAAGATGAAATATCTGC6320.2769900993570499No Hit
CTTATGAAGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT5870.25726770304207003No Hit
CGTCTGAAGCGGGGCTAATTTTGTGTATTTAGTAGAGACAGGGCTTCACCAT5730.25113184641074304No Hit
CCACTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC5690.24937874451607814No Hit
GTAATGAAGCGGGGAATCCCAGGAATGGAGGAGCCTGGTGGGCTGCCGTCTA5680.2489404690424119No Hit
GGGTTGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5640.24718736714774703No Hit
TTCTTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5630.24674909167408085No Hit
CATCTGAAGCGGGAAGGTGATGGGTCAGGCAGGCGCCTTGGGGAACCAGACC5470.23973668409542134No Hit
AGTCTGAAGCGGGAGCAAATAGCTAACACGTCATAACAGAAACAAAACCTCA5460.2392984086217551No Hit
CTGTTGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAG5380.23579220483242536No Hit
TATGTGAAGCGGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT5290.2318477255694294No Hit
CTCTTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5280.23140945009576316No Hit
ACGCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5210.22834152178009964No Hit
TGTCTGAAGCGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAA5120.22439704251710368No Hit
GGTTTGAAGCGGGGTAGGCGGGCAGCTAAAGCTCTGATTAGACCCCTAGCCT5090.22308221609610504No Hit
AGTCTGAAGCGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC5010.21957601230677531No Hit
CGGGTGAAGCGGGGGGTATGATAAGAAAAAAAATGGAGAAAAAAAATTTGCT4970.21782291041211044No Hit
AGGTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC4970.21782291041211044No Hit
ATACTGAAGCGGGCAGAGTGTGTTGAAGCGGCCAAATCAGTAAATTTGTGCT4960.2173846349384442No Hit
AGTATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4930.21606980851744556No Hit
ATCCTGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4880.21387843114911448No Hit
AGTTTGAAGCGGGAGGTCACTAAAATGTGTTCCTATTATTTTAACTTGGTTT4870.21344015567544825No Hit
CTATTGAAGCGGGGTTTTTAAAGTTCTTTAAGTCAGAGAAAAATAAATAATG4850.21256360472811578No Hit
GGTATGAAGCGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT4820.21124877830711714No Hit
TGGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC4780.20949567641245231No Hit
GAATTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4760.20861912546511985No Hit
TCCATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4750.20818084999145361No Hit
CGTATGAAGCGGGGTGGCATAGGGCCAAGGAGAAGTTGTTTATAATAAGGGG4660.20423637072845766No Hit
CTGGTGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG4590.20116844241279414No Hit
TCGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC4430.19415603483413463No Hit
TGGTTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4330.18977328009747246No Hit
GGTATGAAGCGGGAAGTATGGAGAGGAGGGACAGGATGCAAGGAATTAACCT4180.1831991479924792No Hit
ATCCTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGAAGAGC4160.18232259704514675No Hit
ACTCTGAAGCGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCT4100.17969294420314944No Hit
TCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4030.17662501588748591No Hit
AGGTTGAAGCGGGGAAGTAGGTCATAAAAGAGAATCATAGATGTTTGCATTG3980.17443363851915483No Hit
GGGGTGAAGCGGGATGTGGCTGATCAGGCACTGATCACGTGGCTGATCGTAC3980.17443363851915483No Hit
AGTGTGAAGCGGGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCC3940.17268053662448996No Hit
GTACTGAAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGC3870.1696126083088264No Hit
CTTGTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC3840.16829778188782776No Hit
CCGCTGAAGCGGGGCCTGGAAAAAGAAGAAAAAAAAAAAAAAAAAAAAAAAA3770.16522985357216427No Hit
CGTTTGAAGCGGGAGTACTTAAAAATTTGTGTCATAGAAGACATGTATATTT3410.14945193652018038No Hit
GCATTGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATT3380.14813711009918173No Hit
CGATTGAAGCGGGACTGGACCCCTTCCTTTCACCGTATACAGAAATCAACTC3330.14594573273085065No Hit
CGAATGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG3180.13937160062585738No Hit
GGTCTGAAGCGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3120.13674194778386006No Hit
TTGTTGAAGCGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGG3100.13586539683652762No Hit
GAGATGAAGCGGGAATTTTAAAAAATTAAAATTCAGATGGAATTGTGATTTT3070.13455057041552898No Hit
TTTATGAAGCGGGGAGGGGATGGATACCCATTTTACATGATGTAATTAGTAC2930.12841471378420194No Hit
ACCCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2910.1275381628368695No Hit
CTCATGAAGCGGGAGACTCCTGTGAAACATCTGAGTCAAGATGTCTTTCAGG2860.1253467854685384No Hit
TCTCTGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2860.1253467854685384No Hit
TTCTTGAAGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.12490850999487219No Hit
GGTTTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG2700.11833437788987891No Hit
TACATGAAGCGGGTGGAATGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAA2640.11570472504788158No Hit
TCCATGAAGCGGGGGGAACTAAGCGTGCACAAGTAGGGCGGGACACGTGAAA2620.11482817410054917No Hit
CGATTGAAGCGGGGGCAGGAGAATGGCATGAACCCGGGAGGCTGAGCTTGCA2610.11438989862688294No Hit
GAATTGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2560.11219852125855184No Hit
CGTCTGAAGCGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2550.11176024578488564No Hit
GGGCTGAAGCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.11000714389022076No Hit
CCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC2490.10913059294288831No Hit
TACATGAAGCGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2430.106500940100891No Hit
GTATTGAAGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATAC2410.10562438915355857No Hit
ATAATGAAGCGGGGCCACAGGTTAGATTCTAAACTCCTTAATCTCCAGATCT2390.10474783820622616No Hit
TGTATGAAGCGGGGAAGCAGTGATATCAACGCAGAGTGTGGTGAAGCGGGGA2350.10299473631156127No Hit
TTCCTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2340.10255646083789506No Hit
GAATTGAAGCGGGGGAATTCCAGCAGCGATTAAGGCAGAGGCGTTTGTATCT2310.10124163441689639No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAGAAG1100.046.00000434
ATCCTGC1100.046.00000420
TAGAAGA551.8189894E-1246.00000435
GGATGTG351.0147596E-746.00000412
TGTGAGA1100.046.00000432
ACACGTG551.8189894E-1246.00000443
ACACGTC551.8189894E-1246.00000426
GTTGATA1050.046.00000440
CATGATT351.0147596E-746.00000436
CAAGAGT1050.046.00000428
CCGGATT351.0147596E-746.00000432
AACGTGA551.8189894E-1246.0000041
ACCGGCG700.046.00000425
ACAAAAC551.8189894E-1246.00000442
ACCGGAT351.0147596E-746.00000431
GTTGAGT1050.046.00000435
ACTAAGC551.8189894E-1246.00000418
TTCACAC700.046.00000420
CCTATTA551.8189894E-1246.00000432
TGGCACA1450.046.00000435
Produced by FastQC (version 0.11.5)