FastQCFastQC Report
Fri 17 Jun 2016
SRR1528145_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528145_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226620
Sequences flagged as poor quality0
Sequence length52
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGAAGCGGGGGTACCGGTTTTAATGGCGATACGCTAGCCTTTAATCGC22500.9928514694201748No Hit
CGAATGAAGCGGGGGGTTCTTATCTGCCTAGTACCTTTCGAGCCCCGCGCGT15960.7042626423087106No Hit
AGCTTGAAGCGGGGATAGCTTCGTGCTCTAAAGATGGCCTTGATAACTGATA15380.6786691377636572No Hit
GAATTGAAGCGGGGGAAGGCCGAAGTGGGCGGATCACAAGGTCAGGATTTCG14690.6482216927014385No Hit
TATCTGAAGCGGGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTAT14220.6274821286735505No Hit
GTTTTGAAGCGGGAGGCTGATCCTGCCCAAGAGTCCATATCGACGGCATGGT11290.4981908039890566No Hit
TTGTTGAAGCGGGAATTAAATTAGCTGGACATGGTGGCACATGCTTGTGGTC10860.4792163092401377No Hit
TGTGTGAAGCGGGGGGGGGTAGAGGTCGCAGTGAACTGAGATCATGCGCCAC10720.4730385667637455No Hit
TTGATGAAGCAGGGAGGGTATTGGCCTAGTGTGTGAGAAGTTGATAAAGGAG10350.4567116759332804No Hit
TACATGAAGCGGGGCCTCACATAGCCTTGGGAAAGTTGAGTCAATTGTACAT9880.43597211190539226No Hit
GCGATGAAGCGGGAGGGCGGATCACGAGGTCAGGAGATAGAGACCATCCTGG9360.41302621127879263No Hit
AACTTGAAGCGGGAGAGGTTCCCTGCTGAGCTGTGTCTAGATTCCTAACTCA9090.40111199364575056No Hit
TATTTGAAGCGGGCGTTCGCGTCCTTCACCCCCGGCAGCGCAAAAAAAATAA8740.38566763745477006No Hit
TGCGTGAAGCGGGAACTTATTCTCTTAAGCTCACTTAAAAATGCATCGTTTA8570.378166093019151No Hit
ATGCTGAAGCGGGGAGGGAATCCCTAAAGTGGAATAAAAATGAATCCAACTA7910.3490424499161592No Hit
GCACTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT7740.3415409054805401No Hit
GCTGTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA7300.3221251434118789No Hit
TTATTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA6880.30359191598270235No Hit
GGGGTGAAGCGGGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAA6580.29035389639043335No Hit
ATATTGAAGCGGGATCCTCTAATTACTCCTTTTGTAGACTTAGAATGGTTAT6500.2868237578324949No Hit
TGTTTGAAGCGGTCAGCTATTGACAAATATCTATTGAAGATGAAATATCTGC6430.28373488659429885No Hit
CAGTTGAAGCGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTG6180.2727032036007413No Hit
AGTCTGAAGCGGGAGCAAATAGCTAACACGTCATAACAGAAACAAAACCTCA6130.2704968670020298No Hit
AGTTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC6000.2647603918453799No Hit
CGTCTGAAGCGGGGCTAATTTTGTGTATTTAGTAGAGACAGGGCTTCACCAT5660.24975730297414175No Hit
AGGTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC5620.24799223369517254No Hit
GTAATGAAGCGGGGAATCCCAGGAATGGAGGAGCCTGGTGGGCTGCCGTCTA5440.24004942193981113No Hit
TTCTTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5440.24004942193981113No Hit
CATCTGAAGCGGGAAGGTGATGGGTCAGGCAGGCGCCTTGGGGAACCAGACC5390.23784308534109963No Hit
TGTCTGAAGCGGGGTGAAACCCCGTCTCTACTAAAAATACAAAAAAAAAAAA5360.23651928338187275No Hit
GGGTTGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5310.23431294678316122No Hit
CCACTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC5280.23298914482393435No Hit
CTCTTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG5250.23166534286470747No Hit
CTGTTGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAG5220.23034154090548054No Hit
ACGCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT5120.22592886770805756No Hit
TATGTGAAGCGGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCT5070.22372253110934603No Hit
CTTATGAAGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTT4960.21886859059218072No Hit
TCCATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4920.21710352131321153No Hit
CTATTGAAGCGGGGTTTTTAAAGTTCTTTAAGTCAGAGAAAAATAAATAATG4860.21445591739475775No Hit
GGTTTGAAGCGGGGTAGGCGGGCAGCTAAAGCTCTGATTAGACCCCTAGCCT4800.21180831347630394No Hit
AGTTTGAAGCGGGAGGTCACTAAAATGTGTTCCTATTATTTTAACTTGGTTT4780.21092577883681934No Hit
TGGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC4770.21048451151707706No Hit
ATACTGAAGCGGGCAGAGTGTGTTGAAGCGGCCAAATCAGTAAATTTGTGCT4760.21004324419733475No Hit
GAATTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4730.20871944223810784No Hit
TCGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAAC4720.20827817491836553No Hit
AGTATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4720.20827817491836553No Hit
TGGTTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT4670.20607183831965406No Hit
AGTCTGAAGCGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTC4600.20298296708145797No Hit
ATCCTGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4580.20210043244197334No Hit
AGGTTGAAGCGGGGAAGTAGGTCATAAAAGAGAATCATAGATGTTTGCATTG4430.19548142264583884No Hit
CGGGTGAAGCGGGGGGTATGATAAGAAAAAAAATGGAGAAAAAAAATTTGCT4360.19239255140764275No Hit
GGTATGAAGCGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTT4340.19151001676815815No Hit
TCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC4300.18974494748918896No Hit
CGTATGAAGCGGGGTGGCATAGGGCCAAGGAGAAGTTGTTTATAATAAGGGG4220.18621480893125053No Hit
CTGGTGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCG4190.18489100697202365No Hit
AGTGTGAAGCGGGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCC3880.17121172006001234No Hit
GTACTGAAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGC3870.17077045274027006No Hit
ATCCTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGAAGAGC3790.16724031418233165No Hit
CTTGTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC3750.16547524490336246No Hit
GGTATGAAGCGGGAAGTATGGAGAGGAGGGACAGGATGCAAGGAATTAACCT3740.16503397758362015No Hit
ACTCTGAAGCGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCT3730.16459271026387784No Hit
GGGGTGAAGCGGGATGTGGCTGATCAGGCACTGATCACGTGGCTGATCGTAC3650.16106257170593946No Hit
CCGCTGAAGCGGGGCCTGGAAAAAGAAGAAAAAAAAAAAAAAAAAAAAAAAA3620.15973876974671256No Hit
CGAATGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG3410.15047215603212427No Hit
CGATTGAAGCGGGACTGGACCCCTTCCTTTCACCGTATACAGAAATCAACTC3400.15003088871238196No Hit
GGTCTGAAGCGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3400.15003088871238196No Hit
TCTCTGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.14650075015444358No Hit
GCATTGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATT3200.14120554231753596No Hit
CGTTTGAAGCGGGAGTACTTAAAAATTTGTGTCATAGAAGACATGTATATTT3140.13855793839908218No Hit
TTCTTGAAGCGGGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.13679286912011296No Hit
GGGCTGAAGCGGGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3070.13546906716088608No Hit
TTGTTGAAGCGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGG3060.13502779984114377No Hit
GAGATGAAGCGGGAATTTTAAAAAATTAAAATTCAGATGGAATTGTGATTTT3050.13458653252140146No Hit
TCCATGAAGCGGGGGGAACTAAGCGTGCACAAGTAGGGCGGGACACGTGAAA2870.12664372076604005No Hit
ACCCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT2790.12311358220810167No Hit
GGTTTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG2770.12223104756861707No Hit
CTCATGAAGCGGGAGACTCCTGTGAAACATCTGAGTCAAGATGTCTTTCAGG2770.12223104756861707No Hit
TTCCTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC2650.11693583973170947No Hit
GAATTGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2510.11075809725531727No Hit
GTATTGAAGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATAC2450.10811049333686348No Hit
TTTTTGAAGCGGGGCCACCTGATGTGAAGAGCTGACTCACTGGAAAAGACCC2440.10766922601712119No Hit
CCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGC2410.10634542405789428No Hit
TACATGAAGCGGGTGGAATGAAGCGGGGAAAAAAAAAAAAAAAAAAAAAAAA2400.10590415673815197No Hit
ATAATGAAGCGGGGCCACAGGTTAGATTCTAAACTCCTTAATCTCCAGATCT2360.10413908745918277No Hit
TTTATGAAGCGGGGAGGGGATGGATACCCATTTTACATGATGTAATTAGTAC2340.10325655281969816No Hit
CGTCTGAAGCGGGGGGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2330.10281528549995586No Hit
TGTATGAAGCGGGGAAGCAGTGATATCAACGCAGAGTGTGGTGAAGCGGGGA2290.10105021622098667No Hit
CGATTGAAGCGGGGGCAGGAGAATGGCATGAACCCGGGAGGCTGAGCTTGCA2280.10060894890124437No Hit
TTTGTGAAGCGGGTAAGCTCATGTGTAATTTAGTTAATGTAAATATTAAAGG2270.10016768158150208No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAT700.046.00000440
ATCCTGG700.046.00000446
ACCGGTT2950.046.00000417
GGATGTG700.046.00000412
GAGGTAG351.01472324E-746.00000440
CATGATT351.01472324E-746.00000436
CCGGATT351.01472324E-746.00000432
ACCGGAT351.01472324E-746.00000431
GTTGAGT700.046.00000435
GCCTTTA2950.046.00000441
TTCATCT351.01472324E-746.00000419
CCAACCC351.01472324E-746.00000443
CCGTGAA351.01472324E-746.0000042
TAAATAT351.01472324E-746.00000440
TAGGAAT351.01472324E-746.00000444
GCCCAAC351.01472324E-746.00000441
ATGATTT351.01472324E-746.00000437
AATTCAT351.01472324E-746.00000417
AATTTAG351.01472324E-746.00000427
TAAGTGA700.046.0000041
Produced by FastQC (version 0.11.5)