SRR1528144_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528144_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208276
Sequences flagged as poor quality	0
Sequence length	59
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCATTGAAGCGGGGGTACCGGTTTTAATGGCGATACGCTAGCCTTTAATCGCCGCCTTC	1748	0.8392709673702203	No Hit
AGCTTGAAGCGGGGATAGCTTCGTGCTCTAAAGATGGCCTTGATAACTGATAGGAATCT	1368	0.6568207570723463	No Hit
GAATTGAAGCGGGGGAAGGCCGAAGTGGGCGGATCACAAGGTCAGGATTTCGAGACCAG	1342	0.6443373216309128	No Hit
TATCTGAAGCGGGCCATTCGATGGTGTCCGGGATCTCGACGCTCTCCCTTATGCGACTC	1181	0.5670360483204978	No Hit
TTGATGAAGCAGGGAGGGTATTGGCCTAGTGTGTGAGAAGTTGATAAAGGAGATAGTTG	1018	0.4887745107453571	No Hit
TTGTTGAAGCGGGAATTAAATTAGCTGGACATGGTGGCACATGCTTGTGGTCCCAGCTA	956	0.45900631853886187	No Hit
GTTTTGAAGCGGGAGGCTGATCCTGCCCAAGAGTCCATATCGACGGCATGGTTTGGCAC	913	0.4383606368472604	No Hit
TGTGTGAAGCGGGGGGGGGTAGAGGTCGCAGTGAACTGAGATCATGCGCCACTGCACTC	858	0.41195336956730494	No Hit
TACATGAAGCGGGGCCTCACATAGCCTTGGGAAAGTTGAGTCAATTGTACATATCTCTT	849	0.4076321803760395	No Hit
TGCGTGAAGCGGGAACTTATTCTCTTAAGCTCACTTAAAAATGCATCGTTTACTGTTTC	838	0.40235072692004836	No Hit
ATGCTGAAGCGGGGAGGGAATCCCTAAAGTGGAATAAAAATGAATCCAACTAGGCTGGG	836	0.40139046265532274	No Hit
AACTTGAAGCGGGAGAGGTTCCCTGCTGAGCTGTGTCTAGATTCCTAACTCACAAAAAC	785	0.3769037239048186	No Hit
GCGATGAAGCGGGAGGGCGGATCACGAGGTCAGGAGATAGAGACCATCCTGGCTAACAC	749	0.3596189671397569	No Hit
TATTTGAAGCGGGCGTTCGCGTCCTTCACCCCCGGCAGCGCAAAAAAAATAACCGCCGC	694	0.3332116998598014	No Hit
ATATTGAAGCGGGATCCTCTAATTACTCCTTTTGTAGACTTAGAATGGTTATCCTGAAC	661	0.31736733949182816	No Hit
GCTGTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG	638	0.30632430044748316	No Hit
AGTCTGAAGCGGGAGCAAATAGCTAACACGTCATAACAGAAACAAAACCTCACATATCA	627	0.3010428469914921	No Hit
CGAATGAAGCGGGGGGTTCTTATCTGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGC	624	0.29960245059440355	No Hit
GCACTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	623	0.29912231846204074	No Hit
TGTTTGAAGCGGTCAGCTATTGACAAATATCTATTGAAGATGAAATATCTGCCTACTCC	571	0.27415544757917376	No Hit
GGGGTGAAGCGGGGAACAGGGCTGACGCCGCTACATATATAGGAAAAGGGAAGGTAGAA	567	0.2722349190497225	No Hit
CAGTTGAAGCGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAG	536	0.2573508229464749	No Hit
TTATTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG	517	0.24822831243158117	No Hit
CTTATGAAGCGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGAC	491	0.2357448769901477	No Hit
CGTCTGAAGCGGGGCTAATTTTGTGTATTTAGTAGAGACAGGGCTTCACCATGTTAGCC	485	0.2328640841959707	No Hit
CTGTTGAAGCGGCTACAGTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTC	473	0.2271024986076168	No Hit
AGTTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT	471	0.22614223434289116	No Hit
TTCTTGAAGCGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG	456	0.21894025235744877	No Hit
TATGTGAAGCGGGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACA	455	0.21846012022508593	No Hit
CATCTGAAGCGGGAAGGTGATGGGTCAGGCAGGCGCCTTGGGGAACCAGACCAGACTTG	445	0.2136587989014577	No Hit
AGGTTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT	439	0.2107780061072807	No Hit
CCACTGAAGCGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT	439	0.2107780061072807	No Hit
GTAATGAAGCGGGGAATCCCAGGAATGGAGGAGCCTGGTGGGCTGCCGTCTATGGGGTC	427	0.2050164205189268	No Hit
ACGCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	424	0.2035760241218383	No Hit
AGGTTGAAGCGGGGAAGTAGGTCATAAAAGAGAATCATAGATGTTTGCATTGTGGAGGA	415	0.19925483493057292	No Hit
CTCTTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	413	0.19829457066584724	No Hit
GGTATGAAGCGGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGA	408	0.19589391000403314	No Hit
GGGTTGAAGCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	407	0.1954137778716703	No Hit
AGTTTGAAGCGGGAGGTCACTAAAATGTGTTCCTATTATTTTAACTTGGTTTTAACCCT	405	0.19445351360694463	No Hit
CGGGTGAAGCGGGGGGTATGATAAGAAAAAAAATGGAGAAAAAAAATTTGCTCCTTTCT	404	0.1939733814745818	No Hit
CTATTGAAGCGGGGTTTTTAAAGTTCTTTAAGTCAGAGAAAAATAAATAATGTAATGGC	402	0.19301311720985617	No Hit
ATCCTGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG	394	0.18917206015095356	No Hit
TCGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG	391	0.18773166375386505	No Hit
CGTATGAAGCGGGGTGGCATAGGGCCAAGGAGAAGTTGTTTATAATAAGGGGAAAAGCC	388	0.1862912673567766	No Hit
AGTATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	388	0.1862912673567766	No Hit
TGGTTGAAGCGGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGG	379	0.18197007816551117	No Hit
TGGTTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	375	0.18004954963605985	No Hit
GGGGTGAAGCGGGATGTGGCTGATCAGGCACTGATCACGTGGCTGATCGTACTCTGAAC	372	0.17860915323897136	No Hit
TCCATGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	367	0.17620849257715723	No Hit
GAATTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCAT	360	0.17284756765061746	No Hit
CTGGTGAAGCGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAAG	345	0.16564558566517507	No Hit
AGTCTGAAGCGGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCCGCCCGT	336	0.16132439647390961	No Hit
GGTTTGAAGCGGGGTAGGCGGGCAGCTAAAGCTCTGATTAGACCCCTAGCCTGGGAACC	333	0.15988400007682116	No Hit
GGTATGAAGCGGGAAGTATGGAGAGGAGGGACAGGATGCAAGGAATTAACCTATTTTCT	328	0.157483339415007	No Hit
ATACTGAAGCGGGCAGAGTGTGTTGAAGCGGCCAAATCAGTAAATTTGTGCTACCATGT	324	0.1555628108855557	No Hit
ATCCTGAAGCGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGAAGAGCTAGCATC	319	0.15316215022374158	No Hit
CTTGTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC	303	0.14548003610593635	No Hit
TCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC	299	0.14355950757648506	No Hit
GAGATGAAGCGGGAATTTTAAAAAATTAAAATTCAGATGGAATTGTGATTTTAAAGTTA	297	0.14259924331175938	No Hit
ACTCTGAAGCGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTA	296	0.14211911117939657	No Hit
CGAATGAAGCGGGGGGGTCTTATCTGCCTAGTACCTTTCGAGCCCCGCGCGTACTAGGC	292	0.14019858264994525	No Hit
AGTGTGAAGCGGGAGTATGAAGATTACGGTACCGGATTGACCAACAGTTGCCCAATCGA	286	0.1373177898557683	No Hit
CCGCTGAAGCGGGGCCTGGAAAAAGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	274	0.13155620426741438	No Hit
GTACTGAAGCGGGGGGGAATTCCTTCGATTCTGTTTTGCTACCCGTTGTAGCGCCGGAA	274	0.13155620426741438	No Hit
GCATTGAAGCGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTATCTCAT	272	0.13059594000268876	No Hit
ATAATGAAGCGGGGCCACAGGTTAGATTCTAAACTCCTTAATCTCCAGATCTTCCTCAG	272	0.13059594000268876	No Hit
CGTTTGAAGCGGGAGTACTTAAAAATTTGTGTCATAGAAGACATGTATATTTACCTGGT	266	0.12771514720851176	No Hit
ACCCTGAAGCGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTAATGGA	256	0.12291382588488352	No Hit
TCCATGAAGCGGGGGGAACTAAGCGTGCACAAGTAGGGCGGGACACGTGAAATCCTGTC	252	0.12099329735543221	No Hit
GGTCTGAAGCGGGGTCGTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	247	0.11859263669361808	No Hit
CGAATGAAGCGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATGCACAGCG	246	0.11811250456125527	No Hit
CTCATGAAGCGGGAGACTCCTGTGAAACATCTGAGTCAAGATGTCTTTCAGGAAATTGG	238	0.11427144750235266	No Hit
TTGTTGAAGCGGGGGGAATTCATCTCCTCTAACTTTGGAGAGGTAGGAATGGGAGTATT	232	0.11139065470817569	No Hit
CGATTGAAGCGGGACTGGACCCCTTCCTTTCACCGTATACAGAAATCAACTCAAGATGG	230	0.11043039044345004	No Hit
CGATTGAAGCGGGGGCAGGAGAATGGCATGAACCCGGGAGGCTGAGCTTGCAGTGAGCC	225	0.1080297297816359	No Hit
GTATTGAAGCGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTATTATA	222	0.10658933338454743	No Hit
CCGTTGAAGCGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTAGCATC	222	0.10658933338454743	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAGACC	50	3.6379788E-12	53.000004	38
ACTGTTT	100	0.0	53.000004	52
CAGCTAA	50	3.6379788E-12	53.000004	23
ACAGTAG	50	3.6379788E-12	53.000004	35
AAACTTA	25	1.4745148E-5	53.000004	42
CCAATTG	25	1.4745148E-5	53.000004	41
GTTGAGC	25	1.4745148E-5	53.000004	35
GAGAACG	25	1.4745148E-5	53.000004	51
TGCATCG	100	0.0	53.000004	42
TAGGATT	50	3.6379788E-12	53.000004	39
CACATTG	25	1.4745148E-5	53.000004	20
GTGTCAT	25	1.4745148E-5	53.000004	29
GTCATAG	25	1.4745148E-5	53.000004	31
CACATGT	25	1.4745148E-5	53.000004	29
AGCTCAC	100	0.0	53.000004	28
AGCCAAT	25	1.4745148E-5	53.000004	39
AATTTTA	50	3.6379788E-12	53.000004	14
TGACTCA	25	1.4745148E-5	53.000004	33
GTACTTA	25	1.4745148E-5	53.000004	15
GATACAG	50	3.6379788E-12	53.000004	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content