FastQCFastQC Report
Fri 17 Jun 2016
SRR1528143_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528143_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1881725
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTACTTCGGGCGGATTGCAAATCCGTCTAGTCCGGTTCGACTCCGGAAC31130.16543331251909818No Hit
AACCTACTTCGGGTGGCTTATGCCTGTAATCTTAGCACATTTGGAGGCTGAG23270.12366312824668854No Hit
CCGATACTTCGGGGCATATTGCATATTGAGTGCATATTGCGTATTGCATATT22330.11866771180698561No Hit
CGGCTACTTCGGGCCGGATGAGTATTCAAAAGGAAACTCATCTGGCTATTTT22210.11802999907000225No Hit
AGTGTACTTCGGGTTGTCACATCTCAATATCATGGCAATTATACAGATTCAG19710.10474431704951574No Hit
GCGGTACTTCGGGCTGGCCAATATGGTGAAACCCCATCTCTACCAAAAGTAC19310.10261860792623789No Hit
TGCATACTTCGGGATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAG19250.1022997515577462No Hit
CGAATACTTCGGGGAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCG19110.10155575336459896No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACTC206.312484E-446.00000424
TTAGGTG800.046.00000426
GAAATGC551.8189894E-1246.00000429
AGCAATT700.046.00000443
AGACTAA1100.046.00000435
TGCCCAT351.0200347E-746.00000421
CCCTCGA405.6152203E-946.00000422
ATGATCC800.046.00000418
GTCTGTG405.6152203E-946.00000424
CCCTAGT405.6152203E-946.00000426
TAGACCT700.046.00000422
TCCTGCA351.0200347E-746.00000428
TTTAAGG405.6152203E-946.00000422
GTTTACA351.0200347E-746.00000413
ATGTGTC351.0200347E-746.00000427
CATGACA206.312484E-446.00000426
GACGTGA351.0200347E-746.00000440
CTATAAT700.046.00000415
CTATAAC405.6152203E-946.00000431
TTATGAA405.6152203E-946.00000438