##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528143_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1881725
Sequences flagged as poor quality	0
Sequence length	52
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.751046513172753	31.0	31.0	34.0	27.0	34.0
2	31.55647132285536	31.0	31.0	34.0	28.0	34.0
3	31.855431053953154	31.0	31.0	34.0	30.0	34.0
4	35.62897182106843	37.0	35.0	37.0	33.0	37.0
5	35.24208106923169	37.0	35.0	37.0	33.0	37.0
6	35.69845646946286	37.0	35.0	37.0	33.0	37.0
7	35.540650732705366	37.0	35.0	37.0	33.0	37.0
8	35.572028325074065	37.0	35.0	37.0	33.0	37.0
9	37.50929067743693	39.0	37.0	39.0	35.0	39.0
10	37.27385032350636	39.0	37.0	39.0	33.0	39.0
11	37.76074771818411	39.0	37.0	39.0	35.0	39.0
12	37.59542759967583	39.0	37.0	39.0	35.0	39.0
13	37.453660338253464	39.0	37.0	39.0	35.0	39.0
14	37.54514129322829	40.0	37.0	40.0	32.0	40.0
15	36.73967662649962	39.0	36.0	40.0	31.0	40.0
16	36.682693804886476	38.0	35.0	40.0	31.0	40.0
17	36.49868020034808	39.0	36.0	40.0	31.0	40.0
18	36.69432196521809	39.0	36.0	40.0	31.0	40.0
19	36.85759502584065	39.0	36.0	40.0	31.0	40.0
20	36.944587014574395	39.0	36.0	40.0	31.0	40.0
21	37.020591212849915	39.0	36.0	40.0	31.0	40.0
22	37.04545563246489	39.0	36.0	40.0	31.0	40.0
23	36.97046327173206	39.0	36.0	40.0	31.0	40.0
24	36.99967689221326	39.0	36.0	40.0	31.0	40.0
25	37.09315654519125	39.0	36.0	40.0	31.0	40.0
26	36.83637300880841	39.0	36.0	40.0	31.0	40.0
27	36.4785757748874	39.0	35.0	40.0	30.0	40.0
28	36.110827033705775	38.0	35.0	40.0	30.0	40.0
29	35.993732878077296	38.0	35.0	40.0	30.0	40.0
30	35.83311323386786	38.0	35.0	40.0	30.0	40.0
31	35.75778235395714	38.0	35.0	40.0	30.0	40.0
32	35.66051149875779	38.0	35.0	40.0	30.0	40.0
33	35.50687002617279	38.0	35.0	40.0	29.0	40.0
34	35.28370192243819	38.0	35.0	40.0	27.0	40.0
35	35.11722222960316	38.0	34.0	40.0	26.0	40.0
36	34.86052531586709	37.0	34.0	40.0	25.0	40.0
37	34.63309410248575	37.0	33.0	40.0	24.0	40.0
38	34.25083952224688	37.0	33.0	40.0	22.0	40.0
39	33.68347394013472	36.0	33.0	40.0	21.0	40.0
40	33.12700899440673	35.0	33.0	40.0	18.0	40.0
41	32.52871328169632	35.0	33.0	39.0	15.0	40.0
42	31.983235594999268	35.0	31.0	39.0	12.0	40.0
43	31.389330534482987	35.0	30.0	39.0	10.0	40.0
44	30.87101090754494	35.0	29.0	39.0	9.0	40.0
45	30.487757244018123	35.0	26.0	38.0	9.0	40.0
46	30.02743705908143	35.0	25.0	38.0	8.0	40.0
47	29.47477341269314	35.0	23.0	38.0	8.0	40.0
48	29.00105754028883	35.0	23.0	37.0	8.0	40.0
49	28.696740490773095	34.0	21.0	37.0	8.0	40.0
50	28.26076552099802	34.0	20.0	36.0	8.0	40.0
51	27.82895587825001	33.0	19.0	35.0	8.0	39.0
52	27.028956409677292	33.0	15.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	14.0
15	31.0
16	115.0
17	354.0
18	922.0
19	2095.0
20	4057.0
21	6663.0
22	10286.0
23	14867.0
24	20472.0
25	28227.0
26	37756.0
27	49263.0
28	63321.0
29	78303.0
30	90699.0
31	93409.0
32	90493.0
33	96699.0
34	119837.0
35	160162.0
36	221902.0
37	313720.0
38	325119.0
39	52932.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.44615977361198	21.461371879525434	38.00980483333112	6.082663513531467
2	20.1392339475747	22.605534815129737	34.392432475521126	22.862798761774435
3	19.70394186185548	22.755131594680414	36.0164742457054	21.524452297758707
4	21.50494891655263	20.673796649350994	36.62357677131355	21.19767766278282
5	0.0	0.0	100.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	0.0	100.0
8	0.0	0.0	100.0	0.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	0.0	100.0
11	99.11655528836573	0.518513597895548	0.27591704420146407	0.0890140695372597
12	98.00698826874277	1.470671856939776	0.33389576053886727	0.18844411377858084
13	91.34028617359073	5.719114110722874	1.4262445362632692	1.5143551794231358
14	39.022226946020275	39.668336234040574	13.035432913948638	8.274003905990513
15	28.028325074067677	43.927088177071575	19.768297705562716	8.276289043298037
16	36.817654014268825	32.406435584370726	18.67260093796915	12.103309463391303
17	18.57896345108876	34.417781556816216	23.24882753856169	23.754427453533328
18	18.088084071795826	44.899068673690365	20.255244522977588	16.757602731536224
19	23.766703423720255	42.336473182850845	23.087911357929556	10.808912035499342
20	16.144707648567138	43.248933824017854	25.286319733223507	15.3200387941915
21	23.729078372238238	41.2304135832813	17.883962853233072	17.156545191247393
22	22.514023037372624	42.31898922531188	20.22739773346265	14.939590003852848
23	15.017869242317555	44.366259681940775	22.08330122626845	18.532569849473223
24	15.758413158139472	44.38570992041876	21.171053156013762	18.684823765428
25	18.47953340684744	39.56933133162391	23.993038302621265	17.958096958907387
26	17.006204413503568	42.196176380714505	18.734724787096944	22.062894418684984
27	20.0759409584291	44.792836360254555	17.864831471123573	17.266391210192776
28	20.875579587878143	46.28056703290863	17.21133534389988	15.632518035313343
29	22.12778168967304	45.87838286678447	14.555368079820378	17.438467363722115
30	23.88584942007998	47.5484462394877	16.72449481194117	11.841209528491145
31	18.743174480861974	51.56162563605202	16.488806812897742	13.206393070188257
32	17.945927274176622	53.98036376197373	16.369395102897606	11.704313860952052
33	18.570354329139484	53.526312293241574	16.57208146780215	11.33125190981679
34	16.34484316252375	57.70896384965922	14.942406568441191	11.003786419375839
35	16.917190343966308	53.04478603409106	17.12646640715301	12.911557214789621
36	15.290066295553281	54.11550571948611	19.575177031712922	11.019250953247685
37	14.392591903705377	55.294158285615595	18.088509213620483	12.22474059705855
38	15.302501693924459	54.58714743121338	16.659820111865443	13.450530762996719
39	17.071676254500527	54.7401453453613	17.151709202991935	11.036469197146236
40	18.348855438493935	53.83113898151962	15.660417967556365	12.159587612430084
41	18.797326920777476	54.736478497123656	15.69496074080963	10.771233841289241
42	18.876615871075742	55.80209648062283	13.199750229178015	12.121537419123412
43	18.159135899241388	53.809137892093695	14.88171757297161	13.150008635693315
44	18.091060064568413	52.86516361317408	17.66055082437657	11.383225497880932
45	18.311974385205065	53.750149463922725	15.666263667645378	12.271612483226827
46	20.16569902615951	53.775445402489744	12.17446757629303	13.884387995057725
47	21.81817215586762	54.76586642575296	12.632770463271731	10.78319095510768
48	21.539225976165486	55.22586988003029	12.823659142542082	10.41124500126214
49	20.219692037890766	55.58415815275877	12.906136656525263	11.2900131528252
50	18.07267268065206	57.516799744914906	12.684797194063957	11.725730380369075
51	17.555859650055137	57.10345560589353	13.028524359297986	12.312160384753351
52	19.75394916898059	54.27992932017165	13.449095909338507	12.517025601509255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.0
4	4.0
5	87.5
6	171.0
7	270.5
8	370.0
9	3983.5
10	7597.0
11	12002.0
12	16407.0
13	19360.0
14	23643.5
15	24974.0
16	22075.0
17	19176.0
18	20926.0
19	22676.0
20	28067.0
21	33458.0
22	44501.5
23	55545.0
24	71392.5
25	87240.0
26	101722.5
27	116205.0
28	119828.0
29	123451.0
30	117608.5
31	111766.0
32	104607.0
33	97448.0
34	91106.0
35	84764.0
36	83804.5
37	82845.0
38	84833.5
39	85295.0
40	83768.0
41	88608.5
42	93449.0
43	99566.0
44	105683.0
45	115274.0
46	124865.0
47	124588.0
48	124311.0
49	118973.5
50	113636.0
51	104414.5
52	95193.0
53	80160.0
54	65127.0
55	51755.0
56	38383.0
57	28477.0
58	18571.0
59	13857.5
60	9144.0
61	7542.5
62	5941.0
63	4774.5
64	3970.0
65	4332.0
66	2447.0
67	562.0
68	933.0
69	1304.0
70	749.5
71	195.0
72	249.5
73	304.0
74	175.5
75	47.0
76	34.0
77	21.0
78	19.0
79	17.0
80	14.0
81	11.0
82	9.5
83	8.0
84	10.0
85	12.0
86	6.0
87	0.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1881725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.940360691635455
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.55516574103646	34.17104178729649
2	5.930515225875473	4.737338344174045
3	1.8854595275690123	2.2591780080176074
4	0.9854890470335815	1.5744315198470935
5	0.6086930751685931	1.2155710486367186
6	0.4536843804073786	1.0872190676179108
7	0.3729238967821636	1.0426300463606888
8	0.3163678060155635	1.0108675426786369
9	0.2496724107659617	0.8974805526668409
>10	3.2201047624685155	27.887514892796624
>50	0.26920411216090884	7.221957750425034
>100	0.13427439979928615	10.180825605853595
>500	0.013156063580592878	3.545270034981972
>1k	0.005289551336527034	3.168673798646754
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTACTTCGGGCGGATTGCAAATCCGTCTAGTCCGGTTCGACTCCGGAAC	3113	0.16543331251909818	No Hit
AACCTACTTCGGGTGGCTTATGCCTGTAATCTTAGCACATTTGGAGGCTGAG	2327	0.12366312824668854	No Hit
CCGATACTTCGGGGCATATTGCATATTGAGTGCATATTGCGTATTGCATATT	2233	0.11866771180698561	No Hit
CGGCTACTTCGGGCCGGATGAGTATTCAAAAGGAAACTCATCTGGCTATTTT	2221	0.11802999907000225	No Hit
AGTGTACTTCGGGTTGTCACATCTCAATATCATGGCAATTATACAGATTCAG	1971	0.10474431704951574	No Hit
GCGGTACTTCGGGCTGGCCAATATGGTGAAACCCCATCTCTACCAAAAGTAC	1931	0.10261860792623789	No Hit
TGCATACTTCGGGATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAG	1925	0.1022997515577462	No Hit
CGAATACTTCGGGGAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCG	1911	0.10155575336459896	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.004835988255457094	0.0	0.0	0.0	0.0
12	0.030238212278627323	0.0	0.0	0.0	0.0
13	0.07689752753457599	0.0	0.0	0.0	0.0
14	0.36902310380103365	0.0	0.0	0.0	0.0
15	0.6109819447581342	0.0	0.0	0.0	0.0
16	0.7851306646826715	0.0	0.0	0.0	0.0
17	0.9702799293201716	0.0	0.0	0.0	0.0
18	1.1945422418259841	0.0	0.0	0.0	0.0
19	1.4040840186530976	0.0	0.0	0.0	0.0
20	1.6149543636822596	0.0	0.0	0.0	0.0
21	1.818597297692277	0.0	0.0	0.0	0.0
22	2.012674540647544	0.0	0.0	0.0	0.0
23	2.193147245213833	0.0	0.0	0.0	0.0
24	2.3908381936786727	0.0	0.0	0.0	0.0
25	2.589645139433233	0.0	0.0	0.0	0.0
26	2.823845142090369	0.0	0.0	0.0	0.0
27	3.0480011691400177	0.0	0.0	0.0	0.0
28	3.288312585526578	0.0	0.0	0.0	0.0
29	3.5875061446279344	0.0	0.0	0.0	0.0
30	3.950470977427626	0.0	0.0	0.0	0.0
31	4.277033041491185	0.0	0.0	0.0	0.0
32	4.530895853538642	0.0	0.0	0.0	0.0
33	4.737142781224674	0.0	0.0	0.0	0.0
34	4.945302847121657	0.0	0.0	0.0	0.0
35	5.1392738046207604	0.0	0.0	0.0	0.0
36	5.332394478470552	0.0	0.0	0.0	0.0
37	5.531413995137441	0.0	0.0	0.0	0.0
38	5.7322403645591145	0.0	0.0	0.0	0.0
39	5.951507260625224	0.0	0.0	0.0	0.0
40	6.168010734831072	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACTC	20	6.312484E-4	46.000004	24
TTAGGTG	80	0.0	46.000004	26
GAAATGC	55	1.8189894E-12	46.000004	29
AGCAATT	70	0.0	46.000004	43
AGACTAA	110	0.0	46.000004	35
TGCCCAT	35	1.0200347E-7	46.000004	21
CCCTCGA	40	5.6152203E-9	46.000004	22
ATGATCC	80	0.0	46.000004	18
GTCTGTG	40	5.6152203E-9	46.000004	24
CCCTAGT	40	5.6152203E-9	46.000004	26
TAGACCT	70	0.0	46.000004	22
TCCTGCA	35	1.0200347E-7	46.000004	28
TTTAAGG	40	5.6152203E-9	46.000004	22
GTTTACA	35	1.0200347E-7	46.000004	13
ATGTGTC	35	1.0200347E-7	46.000004	27
CATGACA	20	6.312484E-4	46.000004	26
GACGTGA	35	1.0200347E-7	46.000004	40
CTATAAT	70	0.0	46.000004	15
CTATAAC	40	5.6152203E-9	46.000004	31
TTATGAA	40	5.6152203E-9	46.000004	38
>>END_MODULE