FastQCFastQC Report
Fri 17 Jun 2016
SRR1528142_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528142_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878340
Sequences flagged as poor quality0
Sequence length52
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTACTTCGGGCGGATTGCAAATCCGTCTAGTCCGGTTCGACTCCGGAAC32650.17382369539061085No Hit
CCGATACTTCGGGGCATATTGCATATTGAGTGCATATTGCGTATTGCATATT22750.12111758254629089No Hit
AACCTACTTCGGGTGGCTTATGCCTGTAATCTTAGCACATTTGGAGGCTGAG22730.12101110555064577No Hit
CGGCTACTTCGGGCCGGATGAGTATTCAAAAGGAAACTCATCTGGCTATTTT22320.11882832713992142No Hit
CGAATACTTCGGGGAACTGCCTGCCACATTCGGCCCACGGGAGCTGCCTGCG20210.10759500409936434No Hit
GCGGTACTTCGGGCTGGCCAATATGGTGAAACCCCATCTCTACCAAAAGTAC20010.10653023414291343No Hit
AGTGTACTTCGGGTTGTCACATCTCAATATCATGGCAATTATACAGATTCAG19320.10285677779315779No Hit
TGCATACTTCGGGATGAAAAAACAGTGCTTCGGCTGCTTGAGGAGCTTGAAG19130.10184524633452942No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAG551.8189894E-1246.00000439
ACACGGT551.8189894E-1246.00000420
CATATAG1100.046.00000440
ATTAGCA1100.046.00000425
GATAAGT551.8189894E-1246.00000442
TAGGTCA551.8189894E-1246.00000418
CGACCAG1100.046.00000446
CTCTTGT551.8189894E-1246.00000421
ATATGTG551.8189894E-1246.00000432
CCTCGTG551.8189894E-1246.00000439
TCTAGGT551.8189894E-1246.00000432
TCCTAGG551.8189894E-1246.00000440
CATTACG1100.046.00000424
CTAACTG1150.046.00000445
CCCAACA1100.046.00000435
CTTATGA551.8189894E-1246.00000437
TAACAGT551.8189894E-1246.00000423
AGGCACA551.8189894E-1246.00000444
TAACAAT1100.046.00000415
CCTCACT1100.046.00000434