FastQCFastQC Report
Fri 17 Jun 2016
SRR1528141_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528141_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1779536
Sequences flagged as poor quality0
Sequence length59
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTGTACTTCGGGCGGATTGCAAATCCGTCTAGTCCGGTTCGACTCCGGAACGCGCCTC30980.17409032466890245No Hit
CCGATACTTCGGGGCATATTGCATATTGAGTGCATATTGCGTATTGCATATTGAGTGCA19680.11059062587101356No Hit
CGGCTACTTCGGGCCGGATGAGTATTCAAAAGGAAACTCATCTGGCTATTTTTATATTA18350.10311676751692576No Hit
AGTGTACTTCGGGTTGTCACATCTCAATATCATGGCAATTATACAGATTCAGAGTCAAT17880.1004756296023233No Hit
TAGCTACTTCGGGGACAAGAGAAATAAATAAAATGTCATCTGTTAGGAAGCCATTCTAA17870.10041943517860835No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAAGG251.4790627E-553.00000452
CCTTAGG503.6379788E-1253.00000440
CAACGAC503.6379788E-1253.00000422
CTAAATG503.6379788E-1253.00000428
ACGTGAC251.4790627E-553.00000441
GGATTAG251.4790627E-553.00000449
CTTAGGT503.6379788E-1253.00000441
TGTGTCG503.6379788E-1253.00000428
CTAGGTT251.4790627E-553.00000436
TATAACC251.4790627E-553.00000436
TGTCGAG503.6379788E-1253.00000430
TCTGGCG251.4790627E-553.00000424
ACAAGGT503.6379788E-1253.00000424
TATTTGC203.1387128E-453.034
ATGGGTG203.1387128E-453.027
ACAACCC150.006696867353.025
TTCTACA203.1387128E-453.038
TAATAAC353.3434844E-853.053
CTGTCAA1100.053.038
CGTATAA150.006696867353.030