##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1528141_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1779536 Sequences flagged as poor quality 0 Sequence length 59 %GC 37 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.326076010825293 31.0 30.0 33.0 27.0 34.0 2 31.23348839247984 31.0 31.0 34.0 28.0 34.0 3 31.54811647530592 31.0 31.0 34.0 30.0 34.0 4 35.38739648987152 37.0 35.0 37.0 33.0 37.0 5 34.83493337589125 35.0 35.0 37.0 32.0 37.0 6 35.37831041350105 37.0 35.0 37.0 33.0 37.0 7 35.10421424461208 37.0 35.0 37.0 32.0 37.0 8 35.174471884805925 37.0 35.0 37.0 32.0 37.0 9 37.14710632434522 39.0 37.0 39.0 33.0 39.0 10 36.97184715566305 39.0 37.0 39.0 33.0 39.0 11 37.33439896692171 39.0 37.0 39.0 35.0 39.0 12 37.22745311137285 39.0 37.0 39.0 35.0 39.0 13 37.02037778387175 39.0 37.0 39.0 34.0 39.0 14 36.66919579036333 39.0 36.0 40.0 30.0 40.0 15 35.908444673218185 38.0 34.0 40.0 29.0 40.0 16 35.6816007093984 38.0 34.0 40.0 29.0 40.0 17 35.74679635590401 38.0 34.0 40.0 28.0 40.0 18 35.961521430305424 38.0 35.0 40.0 29.0 40.0 19 36.25726256732092 38.0 35.0 40.0 30.0 40.0 20 36.22807349781066 38.0 35.0 40.0 30.0 40.0 21 36.26605249907841 38.0 35.0 40.0 30.0 40.0 22 36.199473345860945 38.0 35.0 40.0 30.0 40.0 23 36.10704588162307 38.0 35.0 40.0 30.0 40.0 24 36.18705437821994 38.0 35.0 40.0 30.0 40.0 25 36.20877127520882 38.0 35.0 40.0 30.0 40.0 26 35.78942375990146 38.0 35.0 40.0 28.0 40.0 27 35.39941029571754 38.0 34.0 40.0 27.0 40.0 28 35.03517152785895 37.0 34.0 40.0 26.0 40.0 29 34.81866677605848 37.0 33.0 40.0 26.0 40.0 30 34.57722406290179 37.0 33.0 40.0 25.0 40.0 31 34.523675272655346 37.0 33.0 40.0 25.0 40.0 32 34.41992407009467 37.0 33.0 40.0 25.0 40.0 33 34.172049905143815 36.0 33.0 40.0 24.0 40.0 34 33.93920887242517 36.0 33.0 40.0 22.0 40.0 35 33.77755886927828 36.0 33.0 40.0 22.0 40.0 36 33.35812818622382 35.0 32.0 40.0 21.0 40.0 37 33.28653986207641 35.0 32.0 39.0 21.0 40.0 38 33.07128150259393 35.0 32.0 39.0 20.0 40.0 39 32.41479857670764 35.0 31.0 39.0 17.0 40.0 40 31.886210225586893 35.0 30.0 38.0 15.0 40.0 41 31.24193272853148 35.0 29.0 38.0 12.0 40.0 42 30.807968481671626 35.0 28.0 38.0 10.0 40.0 43 30.260742687981587 35.0 26.0 38.0 10.0 40.0 44 29.729382265938987 34.0 24.0 38.0 9.0 40.0 45 29.380976276962084 34.0 23.0 37.0 9.0 40.0 46 28.91895696406254 34.0 23.0 37.0 8.0 40.0 47 28.33567682811699 33.0 20.0 36.0 8.0 40.0 48 27.86433486032314 33.0 20.0 36.0 8.0 39.0 49 27.449473345860945 33.0 19.0 35.0 8.0 39.0 50 26.821906384585645 33.0 17.0 35.0 8.0 39.0 51 26.39190834015159 33.0 15.0 35.0 8.0 38.0 52 25.67455786227421 31.0 12.0 35.0 8.0 38.0 53 25.36969693223402 31.0 11.0 35.0 8.0 38.0 54 24.950472482714595 31.0 10.0 35.0 8.0 38.0 55 24.490686336213486 30.0 10.0 35.0 8.0 37.0 56 23.856126540851097 28.0 10.0 35.0 8.0 37.0 57 23.16814495463986 27.0 9.0 35.0 8.0 36.0 58 22.566179610864854 25.0 9.0 34.0 8.0 36.0 59 21.850548682353153 24.0 8.0 34.0 8.0 35.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 4.0 14 35.0 15 135.0 16 534.0 17 1533.0 18 3616.0 19 7060.0 20 11901.0 21 18550.0 22 26148.0 23 35985.0 24 46950.0 25 60254.0 26 74990.0 27 87243.0 28 90163.0 29 83662.0 30 84909.0 31 95327.0 32 110561.0 33 127266.0 34 144516.0 35 160383.0 36 185233.0 37 192542.0 38 124248.0 39 5785.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.707361919062045 22.082554103991153 37.10298639645391 6.107097580492892 2 19.59201724494475 23.224986738116 34.053708382409795 23.12928763452945 3 19.63225245232465 23.115688583990433 35.832374281835264 21.419684681849652 4 21.614791720987945 20.93894138696829 36.189546038967464 21.25672085307631 5 0.0 0.0 100.0 0.0 6 0.0 100.0 0.0 0.0 7 0.0 0.0 0.0 100.0 8 0.0 0.0 100.0 0.0 9 0.0 0.0 100.0 0.0 10 0.0 0.0 0.0 100.0 11 99.11746657555678 0.5221585851592775 0.2673730680357127 0.09300177124823548 12 98.04533316549933 1.4590882117585708 0.3010897222646802 0.1944889004774278 13 91.233164150655 5.837645318779726 1.4165490330063568 1.5126414975589142 14 38.707730554481614 39.94063621078753 13.079252119653662 8.27238111507719 15 27.93728252758022 44.05193263862041 19.697044622867985 8.313740210931389 16 36.81903597342229 31.955577184164863 18.976463527571234 12.24892331484162 17 18.359167783062553 34.06657690544052 23.338499474020193 24.235755837476734 18 17.93057291338866 44.75155321387148 20.372838762463925 16.94503511027594 19 23.937082475431797 41.85669747619604 23.318044703787955 10.888175344584205 20 16.24007606477194 42.68966741892268 25.61707096681382 15.453185549491552 21 23.96394340996754 40.608900297605665 18.129388784492136 17.29776750793465 22 22.63106787387274 41.72924852321054 20.55378480682605 15.085898796090666 23 14.824650920239883 44.08132232222332 22.420563562636552 18.673463194900243 24 15.645876228410103 44.256199368824234 21.379842835435753 18.71808156732991 25 18.320225047428092 39.3152484692639 24.282228625888997 18.082297857419015 26 16.86816113863389 41.96425360318645 18.885934310966455 22.281650947213205 27 19.964136718785124 44.7606567105133 18.045659093156868 17.229547477544706 28 20.68842664604706 46.27464687424138 17.25106994182753 15.785856537884033 29 22.07603555084022 45.706858417025565 14.555254852950434 17.661851179183788 30 23.852622256588234 47.53323338218502 16.69401461954127 11.920129741685473 31 18.465094271765224 51.69443045827676 16.52037385026209 13.32010141969592 32 17.788457215813562 54.33646748365866 16.313522176567375 11.561553123960405 33 18.4958326215373 53.812117315974504 16.6038787639025 11.0881712985857 34 16.09211614713049 58.172692207406875 14.908942555812303 10.826249089650336 35 16.836748455777236 53.289846341967795 17.09389413869683 12.779511063558141 36 15.12832558599545 54.295445554346756 19.708058729916115 10.868170129741687 37 14.20505120435889 55.830901987933935 17.9160747520702 12.047972055636976 38 15.107589843644636 55.12762877514139 16.35696046609903 13.407820915114952 39 16.82225029445878 55.36673604804848 17.001679089380602 10.809334568112137 40 17.902588090378615 54.6434014259897 15.483361955026478 11.97064852860521 41 18.132760449915033 55.605899515379285 15.653293892340475 10.6080461423652 42 18.268694760881488 57.04531967883762 12.821151131530916 11.864834428749967 43 17.440276566475752 54.93937745569632 14.539576608733961 13.080769369093966 44 17.350477877379273 53.99311955476034 17.499842655613598 11.156559912246788 45 17.43690490105286 55.004225820663365 15.394069015743431 12.164800262540346 46 19.45793734996089 54.86289684502027 11.694396741622535 13.984769063396302 47 21.333482435870923 55.74571124158207 12.237122485861484 10.683683836685518 48 21.006543278697368 56.29506792781939 12.321357927010187 10.377030866473058 49 19.68350176675268 56.52754425872811 12.620705622139706 11.168248352379496 50 17.259049549995055 58.62033698672013 12.395646955161345 11.724966508123465 51 16.832814846117188 58.17758112227007 12.705390618678129 12.284213412934609 52 17.57064762949443 56.2582605802861 13.566682550957104 12.60440923926237 53 17.35042168295556 56.37064942771599 13.071553483604713 13.20737540572374 54 16.983977845910395 55.753072711088734 13.849846252056716 13.413103190944156 55 18.243800631175766 53.26124338029688 14.767782163440357 13.727173825086988 56 18.381476969277387 52.09886172575323 14.642693376250888 14.876967928718498 57 19.425456973053652 50.46343541237716 14.970925005169889 15.140182609399305 58 19.890634412565973 48.5716501380135 16.046542469497666 15.491172979922856 59 20.778562501685833 45.96507179399574 17.00117333956717 16.255192364751263 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.5 4 56.0 5 109.0 6 197.5 7 286.0 8 2964.0 9 8861.5 10 12081.0 11 14157.0 12 16233.0 13 16738.5 14 14350.5 15 11457.0 16 11121.5 17 10786.0 18 11853.5 19 16057.0 20 19193.0 21 25035.0 22 30877.0 23 40599.5 24 50322.0 25 62688.5 26 84204.5 27 93354.0 28 99622.5 29 105891.0 30 108439.5 31 110607.0 32 110226.0 33 108466.5 34 106707.0 35 103003.0 36 96117.5 37 92936.0 38 87809.5 39 82683.0 40 79400.0 41 76117.0 42 75091.5 43 76133.0 44 78200.0 45 83410.5 46 88621.0 47 88850.5 48 86040.5 49 83001.0 50 79186.5 51 75372.0 52 64967.0 53 45336.0 54 36110.0 55 29093.0 56 22076.0 57 17284.5 58 9638.5 59 6784.0 60 6157.5 61 5531.0 62 4726.5 63 3922.0 64 4991.5 65 3570.5 66 1080.0 67 1246.0 68 1412.0 69 820.5 70 178.5 71 128.0 72 107.5 73 87.0 74 170.0 75 137.5 76 22.0 77 14.0 78 6.0 79 4.5 80 3.0 81 7.0 82 8.5 83 6.0 84 4.5 85 3.0 86 5.0 87 3.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 52 0.0 53 0.0 54 0.0 55 0.0 56 0.0 57 0.0 58 0.0 59 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 59 1779536.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.37203027997636 #Duplication Level Percentage of deduplicated Percentage of total 1 90.82515123416353 51.19998175555699 2 4.135875282492004 4.662953733176901 3 1.2834564912256377 2.1705314455921156 4 0.6412832941668551 1.446017651072678 5 0.3938234444537586 1.1100313567855937 6 0.2849387870204438 0.9637546757913716 7 0.23114335462468294 0.9121014130142556 8 0.19736656066584346 0.8900762987287777 9 0.15234910011243336 0.7729405276198719 >10 1.6708581639284374 19.654631940957064 >50 0.11210482027120415 4.266039182386978 >100 0.061827659654618825 6.9590042895506095 >500 0.007340508554267124 2.9097672353749595 >1k 0.0024812986662311404 2.082168494391816 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTGTACTTCGGGCGGATTGCAAATCCGTCTAGTCCGGTTCGACTCCGGAACGCGCCTC 3098 0.17409032466890245 No Hit CCGATACTTCGGGGCATATTGCATATTGAGTGCATATTGCGTATTGCATATTGAGTGCA 1968 0.11059062587101356 No Hit CGGCTACTTCGGGCCGGATGAGTATTCAAAAGGAAACTCATCTGGCTATTTTTATATTA 1835 0.10311676751692576 No Hit AGTGTACTTCGGGTTGTCACATCTCAATATCATGGCAATTATACAGATTCAGAGTCAAT 1788 0.1004756296023233 No Hit TAGCTACTTCGGGGACAAGAGAAATAAATAAAATGTCATCTGTTAGGAAGCCATTCTAA 1787 0.10041943517860835 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.00398980408376116 0.0 0.0 0.0 0.0 12 0.02860296167090747 0.0 0.0 0.0 0.0 13 0.06782666942393972 0.0 0.0 0.0 0.0 14 0.3208701594123412 0.0 0.0 0.0 0.0 15 0.5286771383102112 0.0 0.0 0.0 0.0 16 0.6820879150520136 0.0 0.0 0.0 0.0 17 0.8311155267440501 0.0 0.0 0.0 0.0 18 1.022345150646011 0.0 0.0 0.0 0.0 19 1.194974420298325 0.0 0.0 0.0 0.0 20 1.371537299610685 0.0 0.0 0.0 0.0 21 1.536805099756341 0.0 0.0 0.0 0.0 22 1.69903840102139 0.0 0.0 0.0 0.0 23 1.846436374425693 0.0 0.0 0.0 0.0 24 2.0119851466899235 0.0 0.0 0.0 0.0 25 2.1771405579881495 0.0 0.0 0.0 0.0 26 2.376237401210203 0.0 0.0 0.0 0.0 27 2.5666803031801546 0.0 0.0 0.0 0.0 28 2.768924034130245 0.0 0.0 0.0 0.0 29 3.0144374713408437 0.0 0.0 0.0 0.0 30 3.3123241114537723 0.0 0.0 0.0 0.0 31 3.5810458456586436 0.0 0.0 0.0 0.0 32 3.7910444070813965 0.0 0.0 0.0 0.0 33 3.961763066327402 0.0 0.0 0.0 0.0 34 4.134673308098291 0.0 0.0 0.0 0.0 35 4.294265471448737 0.0 0.0 0.0 0.0 36 4.463410686830724 0.0 0.0 0.0 0.0 37 4.63946781632965 0.0 0.0 0.0 0.0 38 4.812996196761403 0.0 0.0 0.0 0.0 39 5.001416099477616 0.0 0.0 0.0 0.0 40 5.181350644212873 0.0 0.0 0.0 0.0 41 5.370894435403386 0.0 0.0 0.0 0.0 42 5.5708903894048785 0.0 0.0 0.0 0.0 43 5.7752695084561365 0.0 0.0 0.0 0.0 44 5.980828710405409 0.0 0.0 0.0 0.0 45 6.192288326844751 0.0 0.0 0.0 0.0 46 6.3926214473885326 0.0 0.0 0.0 0.0 47 6.595370928152057 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTAAGG 25 1.4790627E-5 53.000004 52 CCTTAGG 50 3.6379788E-12 53.000004 40 CAACGAC 50 3.6379788E-12 53.000004 22 CTAAATG 50 3.6379788E-12 53.000004 28 ACGTGAC 25 1.4790627E-5 53.000004 41 GGATTAG 25 1.4790627E-5 53.000004 49 CTTAGGT 50 3.6379788E-12 53.000004 41 TGTGTCG 50 3.6379788E-12 53.000004 28 CTAGGTT 25 1.4790627E-5 53.000004 36 TATAACC 25 1.4790627E-5 53.000004 36 TGTCGAG 50 3.6379788E-12 53.000004 30 TCTGGCG 25 1.4790627E-5 53.000004 24 ACAAGGT 50 3.6379788E-12 53.000004 24 TATTTGC 20 3.1387128E-4 53.0 34 ATGGGTG 20 3.1387128E-4 53.0 27 ACAACCC 15 0.0066968673 53.0 25 TTCTACA 20 3.1387128E-4 53.0 38 TAATAAC 35 3.3434844E-8 53.0 53 CTGTCAA 110 0.0 53.0 38 CGTATAA 15 0.0066968673 53.0 30 >>END_MODULE