FastQCFastQC Report
Fri 17 Jun 2016
SRR1528140_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528140_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804982
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGA40580.2248221865924425No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTC35560.1970102748947081No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTC31190.17279950714189948No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGG29530.16360273952870444No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27480.15224528554855396No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC26950.149308968178076No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCC26450.14653885745121004No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTG24650.13656645883449253No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC24090.13346393482040264No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23170.12836693108296926No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCT21150.1171756837464307No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCG21130.11706487931735607No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGT20230.11207868000899732No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAA20140.11158006007816144No Hit
ACTACTTCTGGGGTATCAACGCAGAGTTGAACTTCTGGAGCAGACCTCCTAC19290.10687087184248929No Hit
AGCACTTCTGGGGTGCAATTCAGCAACTAAAAGACAAATATCCTAATAAAAA18900.10471018547553383No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGT18800.10415616333016063No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCC18360.10171846589051858No Hit
GTATCTTCTGGGGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCA18350.10166306367598126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG700.046.00000437
TATTTTA1400.046.00000433
TTTAGTA1400.046.00000436
TCGCCCC1100.046.00000432
ATTTACT551.8189894E-1246.00000436
ATTTAAT700.046.00000441
GCCCCAC351.0199983E-746.00000435
AAGTATT351.0199983E-746.00000433
GTGCACA351.0199983E-746.00000420
CTAGAAC700.046.00000441
TCGTTTA1100.046.00000439
GACAGGT551.8189894E-1246.00000413
CTCACTG1500.046.00000439
CGGCGAC750.046.00000417
CCTTAGC551.8189894E-1246.00000427
TGCCCTA750.046.00000440
GCGACGA750.046.00000419
TTAATGT700.046.00000443
TACTTAC551.8189894E-1246.00000437
AGATATT750.046.00000426
Produced by FastQC (version 0.11.5)