##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528140_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1804982
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.114016649473513	31.0	31.0	34.0	28.0	34.0
2	31.706502336311388	31.0	31.0	34.0	30.0	34.0
3	31.842959098761096	31.0	31.0	34.0	30.0	34.0
4	35.53048839268203	37.0	35.0	37.0	33.0	37.0
5	35.365054055940725	37.0	35.0	37.0	33.0	37.0
6	35.52334538516173	37.0	35.0	37.0	33.0	37.0
7	35.757516141435204	37.0	35.0	37.0	35.0	37.0
8	35.62142891175646	37.0	35.0	37.0	33.0	37.0
9	37.590619186230114	39.0	37.0	39.0	35.0	39.0
10	37.09657547831502	39.0	37.0	39.0	33.0	39.0
11	37.56027428528373	39.0	37.0	39.0	35.0	39.0
12	37.59635719358974	39.0	37.0	39.0	35.0	39.0
13	37.32373951651596	39.0	37.0	39.0	35.0	39.0
14	37.370390951267105	40.0	37.0	40.0	32.0	40.0
15	36.06338567365215	38.0	35.0	40.0	30.0	40.0
16	36.44933966100493	38.0	35.0	40.0	31.0	40.0
17	36.22653577708808	38.0	35.0	40.0	30.0	40.0
18	36.57055582825757	38.0	35.0	40.0	31.0	40.0
19	36.79482343868249	39.0	35.0	40.0	31.0	40.0
20	36.948405025645684	39.0	36.0	40.0	31.0	40.0
21	37.002683129250045	39.0	36.0	40.0	31.0	40.0
22	37.13492378317346	39.0	36.0	40.0	32.0	40.0
23	37.19371661324046	39.0	36.0	40.0	32.0	40.0
24	37.14501751264002	40.0	36.0	40.0	32.0	40.0
25	37.221276444862056	40.0	36.0	40.0	32.0	40.0
26	36.98528960399605	40.0	36.0	40.0	31.0	40.0
27	36.527164259809794	39.0	35.0	40.0	30.0	40.0
28	35.94168916919947	38.0	35.0	40.0	30.0	40.0
29	35.640965394668754	37.0	35.0	40.0	30.0	40.0
30	35.40258961031191	37.0	34.0	40.0	29.0	40.0
31	35.17997076979161	37.0	34.0	40.0	28.0	40.0
32	35.01009483751084	37.0	34.0	40.0	27.0	40.0
33	34.833416621329185	37.0	34.0	40.0	25.0	40.0
34	34.666568974095036	37.0	33.0	40.0	24.0	40.0
35	34.54795560288136	37.0	33.0	40.0	24.0	40.0
36	34.323664169504184	36.0	33.0	40.0	23.0	40.0
37	34.032927198165964	36.0	33.0	40.0	21.0	40.0
38	33.56931426462979	36.0	33.0	40.0	20.0	40.0
39	32.8852448390067	35.0	33.0	40.0	15.0	40.0
40	32.23423169870946	35.0	32.0	40.0	13.0	40.0
41	31.415055108582802	35.0	30.0	39.0	10.0	40.0
42	30.697710004864316	35.0	28.0	39.0	9.0	40.0
43	30.043617055460942	35.0	25.0	38.0	8.0	40.0
44	29.530953771284146	35.0	23.0	38.0	8.0	40.0
45	29.16645927771025	35.0	23.0	38.0	8.0	40.0
46	28.78300116012237	35.0	20.0	38.0	8.0	40.0
47	28.31578819068556	34.0	20.0	37.0	8.0	40.0
48	27.931119534710042	34.0	18.0	37.0	8.0	40.0
49	27.65817775468121	34.0	17.0	36.0	8.0	40.0
50	27.220509678212856	33.0	15.0	35.0	8.0	40.0
51	26.866865708356094	33.0	13.0	35.0	8.0	39.0
52	26.095323388266475	33.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	5.0
14	14.0
15	39.0
16	149.0
17	454.0
18	1135.0
19	2499.0
20	4722.0
21	8035.0
22	12532.0
23	17972.0
24	24926.0
25	33906.0
26	44464.0
27	57610.0
28	72584.0
29	89440.0
30	98941.0
31	92055.0
32	82051.0
33	87569.0
34	113583.0
35	150167.0
36	201989.0
37	269496.0
38	277984.0
39	60658.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15118931933947	24.19641857924345	36.914495546215974	5.737896555201105
2	24.5293304863982	20.770345632255612	34.59740872762166	20.102915153724524
3	21.86935936203242	20.515717054242092	36.97155982718941	20.643363756536075
4	30.524570328125154	20.636937099649746	38.585149325588844	10.253343246636254
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.68475142688403	0.7420018592983199	0.2089771532347691	0.36426956058287563
12	97.87454944149027	1.2396245502725234	0.39119503684801293	0.4946309713891883
13	90.08123072695462	6.5914784745775865	1.9790225054875894	1.348268292980207
14	38.72382106857575	43.2220376712898	11.170693114945191	6.883448145189259
15	24.275034321671907	53.270835941854266	14.24202568225057	8.212104054223255
16	33.024152041405394	40.35691214649232	15.79694423545498	10.821991576647303
17	16.983770475273438	39.67690536526126	27.285978475131607	16.053345684333696
18	16.632908250608594	44.562383447591166	26.16419443517996	12.640513866620276
19	20.64264352774709	44.35102399913129	23.54887749573126	11.457454977390356
20	17.26981210892962	45.06105878064158	25.100638122707043	12.568490987721761
21	19.51897581250118	45.5162433752802	18.134419068999026	16.830361743219598
22	15.995671979000345	44.062046048104634	19.22811418618025	20.71416778671477
23	11.636293325916823	42.22679228934139	30.960364147675712	15.176550237066074
24	12.698464583026311	42.755329416027415	27.40110427694016	17.14510172400611
25	16.369747731556323	39.350253908349224	21.597611499726867	22.682386860367583
26	18.003614440476415	41.19415041258029	23.746940412702177	17.055294734241116
27	26.84774695814141	45.192029615807805	14.905411799120435	13.054811626930352
28	28.368482344976293	44.604987750570366	12.838188968089431	14.188340936363907
29	30.34334968437358	45.867936633163104	10.959666079772541	12.829047602690775
30	30.46445892535217	48.75350557512485	11.196787557992268	9.585247941530719
31	21.515394613353486	54.94553408288836	12.886887514667736	10.652183789090417
32	17.629483285705895	60.18481070725359	11.933637011338618	10.252068995701896
33	15.910352568612874	59.233887096935035	13.908282741877759	10.94747759257433
34	13.208331163413265	60.206971593068516	15.747470057873153	10.837227185645066
35	14.804579768662512	59.1851331481422	13.832714121248854	12.177572961946435
36	14.465518215694118	59.08280525789177	14.48468738192403	11.966989144490084
37	16.584708323961124	58.24024837920822	15.646638027415232	9.52840526941543
38	16.898894282602264	59.49339106982784	13.665898053276987	9.941816594292908
39	15.458713715704645	59.9064699814181	13.225616654348906	11.40919964852835
40	19.643630795210147	57.107106885276416	12.701954922542164	10.547307396971272
41	20.184522615737997	55.97900699286752	12.20931843087632	11.627151960518166
42	18.413923241339802	57.149711188255615	12.973924393705866	11.462441176698714
43	19.583741001295305	54.923594805931586	13.783793965812402	11.708870226960713
44	19.604184418459575	52.8075626238932	13.433374958863856	14.154877998783366
45	20.74081625190722	51.80555817177124	12.973093360487805	14.48053221583373
46	21.82176885974486	53.97488728419453	10.99462487714559	13.208718978915023
47	21.590409211837017	53.88402765235332	12.544723437685251	11.980839698124415
48	21.90321011511472	54.56868821960551	12.548934005990088	10.979167659289677
49	22.362383669200025	53.79327882494119	11.315292894887595	12.52904461097119
50	20.558265955006753	53.395269315705086	13.87803313274038	12.168431596547777
51	20.65627247252327	52.34174080406342	13.50007922516679	13.50190749824652
52	21.47123904836724	50.32188686646183	12.643616390634365	15.563257694536567
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	2.5
6	5.0
7	16.0
8	27.0
9	412.0
10	797.0
11	5148.0
12	9499.0
13	15616.5
14	25284.0
15	28834.0
16	27851.0
17	26868.0
18	23985.0
19	21102.0
20	22495.5
21	23889.0
22	27988.5
23	32088.0
24	39856.0
25	47624.0
26	60270.0
27	72916.0
28	89521.0
29	106126.0
30	118471.5
31	130817.0
32	132814.5
33	134812.0
34	124996.0
35	115180.0
36	101320.0
37	87460.0
38	79761.0
39	68729.5
40	65397.0
41	66157.0
42	66917.0
43	68810.0
44	70703.0
45	78878.0
46	87053.0
47	99415.5
48	111778.0
49	115597.0
50	119416.0
51	107131.0
52	94846.0
53	91672.0
54	88498.0
55	77142.0
56	65786.0
57	53167.0
58	40548.0
59	33981.5
60	27415.0
61	21466.5
62	15518.0
63	11531.5
64	5816.5
65	4088.0
66	5027.5
67	5967.0
68	3273.5
69	580.0
70	689.0
71	798.0
72	454.5
73	111.0
74	78.5
75	46.0
76	44.5
77	43.0
78	42.0
79	41.0
80	28.5
81	16.0
82	13.0
83	10.0
84	9.0
85	8.0
86	10.0
87	12.0
88	7.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1804982.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.11958837477136
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.88475939469518	35.25900371330991
2	5.190215451905936	4.1645861501368815
3	1.5803946477270192	1.9021434820949936
4	0.7900423990438967	1.2678470339303196
5	0.4997718571444273	1.0025320594964728
6	0.3614835661335113	0.8701543126512546
7	0.2669668562423054	0.7497420267503637
8	0.22967805519024317	0.7371671226360459
9	0.1921673712107773	0.6938708248834444
>10	2.630129699809356	23.15943994443634
>50	0.2369054850693681	6.282668568357827
>100	0.09638144559765022	8.282074391858291
>500	0.029197850577388385	8.353317124696638
>1k	0.011905919652915651	7.275453244761275
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGA	4058	0.2248221865924425	No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTC	3556	0.1970102748947081	No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTC	3119	0.17279950714189948	No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGG	2953	0.16360273952870444	No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2748	0.15224528554855396	No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	2695	0.149308968178076	No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCC	2645	0.14653885745121004	No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTG	2465	0.13656645883449253	No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC	2409	0.13346393482040264	No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2317	0.12836693108296926	No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCT	2115	0.1171756837464307	No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCG	2113	0.11706487931735607	No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGT	2023	0.11207868000899732	No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAA	2014	0.11158006007816144	No Hit
ACTACTTCTGGGGTATCAACGCAGAGTTGAACTTCTGGAGCAGACCTCCTAC	1929	0.10687087184248929	No Hit
AGCACTTCTGGGGTGCAATTCAGCAACTAAAAGACAAATATCCTAATAAAAA	1890	0.10471018547553383	No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGT	1880	0.10415616333016063	No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCC	1836	0.10171846589051858	No Hit
GTATCTTCTGGGGATGTAAGCCACGGCTCTGCCCAGGTCAAGGGTCACGGCA	1835	0.10166306367598126	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.03163466450080943	0.0	0.0	0.0	0.0
12	0.054238768032035777	0.0	0.0	0.0	0.0
13	0.14493219322962778	0.0	0.0	0.0	0.0
14	0.46814871284034965	0.0	0.0	0.0	0.0
15	0.8412272255346591	0.0	0.0	0.0	0.0
16	1.077406866107252	0.0	0.0	0.0	0.0
17	1.238516505981777	0.0	0.0	0.0	0.0
18	1.4477706702892328	0.0	0.0	0.0	0.0
19	1.6278278675355211	0.0	0.0	0.0	0.0
20	1.7936466956457184	0.0	0.0	0.0	0.0
21	1.9642855164206623	0.0	0.0	0.0	0.0
22	2.143179267161667	0.0	0.0	0.0	0.0
23	2.325120139702224	0.0	0.0	0.0	0.0
24	2.517919846292096	0.0	0.0	0.0	0.0
25	2.7286698703920593	0.0	0.0	0.0	0.0
26	2.965680544182712	0.0	0.0	0.0	0.0
27	3.1840760738888254	0.0	0.0	0.0	0.0
28	3.4138290575750894	0.0	0.0	0.0	0.0
29	3.6670171780106395	0.0	0.0	0.0	0.0
30	3.9517291585179244	0.0	0.0	0.0	0.0
31	4.220485301238461	0.0	0.0	0.0	0.0
32	4.447301967554247	0.0	0.0	0.0	0.0
33	4.655780500858181	0.0	0.0	0.0	0.0
34	4.854785255476232	0.0	0.0	0.0	0.0
35	5.052294150301776	0.0	0.0	0.0	0.0
36	5.241381908517647	0.0	0.0	0.0	0.0
37	5.429140013584623	0.0	0.0	0.0	0.0
38	5.619945240451151	0.0	0.0	0.0	0.0
39	5.819504017214576	0.0	0.0	0.0	0.0
40	6.025766461937017	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	70	0.0	46.000004	37
TATTTTA	140	0.0	46.000004	33
TTTAGTA	140	0.0	46.000004	36
TCGCCCC	110	0.0	46.000004	32
ATTTACT	55	1.8189894E-12	46.000004	36
ATTTAAT	70	0.0	46.000004	41
GCCCCAC	35	1.0199983E-7	46.000004	35
AAGTATT	35	1.0199983E-7	46.000004	33
GTGCACA	35	1.0199983E-7	46.000004	20
CTAGAAC	70	0.0	46.000004	41
TCGTTTA	110	0.0	46.000004	39
GACAGGT	55	1.8189894E-12	46.000004	13
CTCACTG	150	0.0	46.000004	39
CGGCGAC	75	0.0	46.000004	17
CCTTAGC	55	1.8189894E-12	46.000004	27
TGCCCTA	75	0.0	46.000004	40
GCGACGA	75	0.0	46.000004	19
TTAATGT	70	0.0	46.000004	43
TACTTAC	55	1.8189894E-12	46.000004	37
AGATATT	75	0.0	46.000004	26
>>END_MODULE