FastQCFastQC Report
Fri 17 Jun 2016
SRR1528139_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528139_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1793203
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGA40060.22339913551338025No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTC34650.19322965665348543No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTC31320.17465953380626734No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGG29100.16227945190812196No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26660.14867251504709728No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCC25690.14326319998349324No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC25560.14253824023270092No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTG24290.13545594112880696No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23700.13216573918290345No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC23110.12887553723699993No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGT21550.12017602022749237No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCT21050.11738771349367584No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCG20680.1153243665106516No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAA19420.10829783354143396No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGT19020.10606718815438074No Hit
AGCACTTCTGGGGTGCAATTCAGCAACTAAAAGACAAATATCCTAATAAAAA18980.10584412361567541No Hit
ACTACTTCTGGGGTATCAACGCAGAGTTGAACTTCTGGAGCAGACCTCCTAC18710.1043384379794145No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCC18470.10300005074718255No Hit
ATTTCTTCTGGGGGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGA18140.10115976830286363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCAG700.046.00000437
ATTTAGC206.312404E-446.00000424
ATTTACA700.046.00000435
CTAGATA206.312404E-446.00000446
ACTATTA405.6152203E-946.00000435
TGTCACG206.312404E-446.00000413
CTATGAA750.046.00000424
TTGGCTG850.046.00000417
TAGAGCG206.312404E-446.00000438
TAATAAG351.0199983E-746.00000417
TCCATTC750.046.00000418
ATCTCAC750.046.00000415
ATTAGTA750.046.00000420
GATAAAT1650.046.00000433
TACTTAC405.6152203E-946.00000437
TTCAGAA1600.046.00000446
TTTACGT405.6152203E-946.00000415
CCGATAT800.046.00000427
GCGCGGT1600.046.00000413
GTTTACG405.6152203E-946.00000414
Produced by FastQC (version 0.11.5)