##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528139_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1793203
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145994067598593	31.0	31.0	34.0	28.0	34.0
2	31.7304727908664	31.0	31.0	34.0	30.0	34.0
3	31.865644324708356	31.0	31.0	34.0	30.0	34.0
4	35.567801860692846	37.0	35.0	37.0	33.0	37.0
5	35.49087861218167	37.0	35.0	37.0	33.0	37.0
6	35.58171160766517	37.0	35.0	37.0	33.0	37.0
7	35.75122838853158	37.0	35.0	37.0	35.0	37.0
8	35.62693069329016	37.0	35.0	37.0	33.0	37.0
9	37.59895616949113	39.0	37.0	39.0	35.0	39.0
10	37.046254105084586	39.0	37.0	39.0	33.0	39.0
11	37.54448380913929	39.0	37.0	39.0	35.0	39.0
12	37.61298302534627	39.0	37.0	39.0	35.0	39.0
13	37.32143376962898	39.0	37.0	39.0	35.0	39.0
14	37.32843074654682	40.0	37.0	40.0	31.0	40.0
15	36.085447659857806	38.0	35.0	40.0	30.0	40.0
16	36.436656641774526	38.0	35.0	40.0	31.0	40.0
17	36.26217723258326	38.0	35.0	40.0	30.0	40.0
18	36.51497237066857	38.0	35.0	40.0	30.0	40.0
19	36.81943092890208	39.0	35.0	40.0	31.0	40.0
20	36.9731742585753	39.0	36.0	40.0	31.0	40.0
21	37.070705324494774	39.0	36.0	40.0	32.0	40.0
22	37.07856890714548	39.0	36.0	40.0	31.0	40.0
23	37.18311646812993	39.0	36.0	40.0	32.0	40.0
24	37.253032144157686	40.0	36.0	40.0	32.0	40.0
25	37.25301262601055	40.0	36.0	40.0	32.0	40.0
26	36.897163901688764	40.0	36.0	40.0	31.0	40.0
27	36.40887283815608	39.0	35.0	40.0	30.0	40.0
28	35.87294857302826	38.0	35.0	40.0	30.0	40.0
29	35.573564733050304	37.0	35.0	40.0	29.0	40.0
30	35.21714663649347	37.0	34.0	40.0	29.0	40.0
31	35.05588603186588	37.0	34.0	40.0	28.0	40.0
32	34.978393968781	37.0	34.0	40.0	27.0	40.0
33	34.89273328228873	37.0	34.0	40.0	25.0	40.0
34	34.77135048290684	37.0	34.0	40.0	25.0	40.0
35	34.47593998002457	37.0	33.0	40.0	24.0	40.0
36	34.29962196137303	36.0	33.0	40.0	23.0	40.0
37	34.00893986904996	36.0	33.0	40.0	21.0	40.0
38	33.592816318063264	36.0	33.0	40.0	20.0	40.0
39	32.92064925164635	35.0	33.0	40.0	16.0	40.0
40	32.19384196881223	35.0	31.0	39.0	12.0	40.0
41	31.3578797269467	35.0	30.0	39.0	10.0	40.0
42	30.634571211402168	35.0	28.0	39.0	9.0	40.0
43	30.025980884484355	35.0	25.0	38.0	8.0	40.0
44	29.43638283005326	35.0	23.0	38.0	8.0	40.0
45	29.062827242649046	35.0	22.0	38.0	8.0	40.0
46	28.681200622573126	35.0	20.0	38.0	8.0	40.0
47	28.318509393526554	34.0	20.0	37.0	8.0	40.0
48	27.928592022208306	34.0	18.0	37.0	8.0	40.0
49	27.485927137083756	33.0	15.0	36.0	8.0	40.0
50	27.056881457369858	33.0	15.0	35.0	8.0	39.0
51	26.722129619457473	33.0	12.0	35.0	8.0	39.0
52	25.7957632236841	33.0	10.0	35.0	8.0	38.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	1.0
14	10.0
15	38.0
16	132.0
17	456.0
18	1126.0
19	2544.0
20	4804.0
21	8034.0
22	12459.0
23	17859.0
24	25245.0
25	33850.0
26	44736.0
27	57702.0
28	72663.0
29	89416.0
30	99153.0
31	91406.0
32	81342.0
33	87839.0
34	113032.0
35	149356.0
36	199847.0
37	268356.0
38	278391.0
39	53403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.18190968897554	24.172388736802247	36.92342696281458	5.722274611407633
2	24.55940571145598	20.784317224541784	34.55241821478104	20.103858849221197
3	21.904937700862646	20.461096707957772	37.04694895112265	20.587016640056927
4	30.572054586123265	20.609378860062137	38.524863052314764	10.29370350149983
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.68280389894507	0.7493853177805302	0.21191131177005618	0.35589947150434165
12	97.89627833547011	1.2368371009863357	0.37809439310552123	0.48879017043803746
13	90.07742012477115	6.592505143031771	1.9712213285389328	1.358853403658147
14	38.68390806841167	43.24920268368946	11.187355809688029	6.879533438210844
15	24.283809473885558	53.28732998996767	14.120933324336397	8.307927211810375
16	33.01087495392323	40.39475731414681	15.815944987823464	10.778422744106496
17	16.91626659112214	39.76705370223003	27.24499122519871	16.07168848144912
18	16.62388474701414	44.657241818132135	26.01155585842763	12.707317576426094
19	20.711988547866582	44.36692332100716	23.44910197005024	11.471986161076018
20	17.242554245113354	45.156627554158675	25.08818020045695	12.512638000271023
21	19.493442739054085	45.572308322036044	18.148586635199695	16.78566230371018
22	16.002928837393203	44.04197405424818	19.29669981591599	20.65839729244263
23	11.618762627544122	42.1983456418487	31.01589725201218	15.166994478595006
24	12.638390633966148	42.86190687836235	27.329532685368026	17.170169802303477
25	16.326260886246565	39.373846686627225	21.60731384009507	22.69257858703114
26	17.906394312300392	41.2766429679183	23.777174140351093	17.039788579430216
27	26.740196174108565	45.18451062149684	14.97527050757778	13.100022696816813
28	28.285308467585658	44.64212919563485	12.845394525884688	14.227167810894805
29	30.226750680207427	45.8824795631058	11.077384992106303	12.813384764580475
30	30.420928361150413	48.643405124796246	11.331845864634401	9.603820649418946
31	21.49996403084313	54.926631284913086	12.889951667491076	10.683453016752704
32	17.63319601852105	60.26506759134354	11.863074063561125	10.238662326574293
33	15.943872500770967	59.29897507421078	13.793697646055689	10.96345477896256
34	13.257729325681474	60.31313799943453	15.637102993916471	10.79202968096752
35	14.769605002891472	59.15587917263132	13.984585125052767	12.089930699424437
36	14.44292698595753	59.11126626489025	14.479398038035852	11.966408711116365
37	16.53861832709403	58.280573922751636	15.674410538014937	9.506397212139394
38	16.892789048423406	59.483560979989434	13.658353237196236	9.96529673439092
39	15.453688176966022	59.97391260219841	13.147368145157017	11.425031075678548
40	19.679868927277056	57.03135673986716	12.752265080975217	10.536509251880572
41	20.249631525265126	55.86985968682854	12.188748290070896	11.691760497835437
42	18.399366942839155	57.10251432771415	13.052565716207257	11.445553013239438
43	19.582055127054772	54.85318728554436	13.862847653054338	11.70190993434653
44	19.582445489997507	52.752365460017636	13.484530195410112	14.180658854574746
45	20.70931177340212	51.83278189920494	13.007785510062162	14.450120817330776
46	21.844431444738827	54.01072828898903	10.928099049577767	13.216741216694372
47	21.740260305163442	53.957750461046516	12.389506374905686	11.912482858884355
48	21.89975145033775	54.628115165990685	12.481799327795013	10.990334055876552
49	22.247341767775318	53.70903350039008	11.454921723865063	12.588703007969537
50	20.55461651580998	53.35820874714129	13.917275400498438	12.169899336550296
51	20.545247805184356	52.31036307657304	13.588589802716145	13.555799315526462
52	21.63302202818086	50.139108622950104	12.7391042731916	15.488765075677433
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	4.5
6	9.0
7	14.5
8	20.0
9	385.5
10	751.0
11	4977.5
12	9204.0
13	15268.5
14	24916.5
15	28500.0
16	27500.0
17	26500.0
18	23745.5
19	20991.0
20	22323.5
21	23656.0
22	27596.5
23	31537.0
24	39440.0
25	47343.0
26	59807.0
27	72271.0
28	89420.0
29	106569.0
30	118099.0
31	129629.0
32	132192.0
33	134755.0
34	124763.0
35	114771.0
36	101264.5
37	87758.0
38	79746.0
39	68377.5
40	65021.0
41	65780.5
42	66540.0
43	68201.0
44	69862.0
45	78376.5
46	86891.0
47	98887.5
48	110884.0
49	114714.0
50	118544.0
51	106186.5
52	93829.0
53	90885.5
54	87942.0
55	76545.0
56	65148.0
57	52435.0
58	39722.0
59	33404.0
60	27086.0
61	21250.5
62	15415.0
63	11373.5
64	5708.5
65	4085.0
66	4980.5
67	5876.0
68	3228.5
69	581.0
70	695.0
71	809.0
72	466.0
73	123.0
74	93.5
75	64.0
76	54.0
77	44.0
78	39.5
79	35.0
80	22.5
81	10.0
82	6.0
83	2.0
84	6.5
85	11.0
86	13.0
87	15.0
88	8.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	1793203.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.674033966169176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.02383759505483	35.8028456017382
2	5.173466956770775	4.208515414450967
3	1.5472109833924679	1.8879393627400578
4	0.7652207384544008	1.2449845723004542
5	0.4763487247724353	0.9687512105567703
6	0.3535838379740272	0.8629008621386426
7	0.2711835914966703	0.772109142812232
8	0.23816393257031784	0.7749670306305227
9	0.18361312142864056	0.6721457703860585
>10	2.6051546617680943	23.180695385177923
>50	0.22784883764112568	6.134840356668274
>100	0.09439358992122168	8.242709808783093
>500	0.029313847753609377	8.537242306949782
>1k	0.0106595810013125	6.709353174667054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGA	4006	0.22339913551338025	No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTC	3465	0.19322965665348543	No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTC	3132	0.17465953380626734	No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGG	2910	0.16227945190812196	No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2666	0.14867251504709728	No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCC	2569	0.14326319998349324	No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCC	2556	0.14253824023270092	No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTG	2429	0.13545594112880696	No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2370	0.13216573918290345	No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGC	2311	0.12887553723699993	No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGT	2155	0.12017602022749237	No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCT	2105	0.11738771349367584	No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCG	2068	0.1153243665106516	No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAA	1942	0.10829783354143396	No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGT	1902	0.10606718815438074	No Hit
AGCACTTCTGGGGTGCAATTCAGCAACTAAAAGACAAATATCCTAATAAAAA	1898	0.10584412361567541	No Hit
ACTACTTCTGGGGTATCAACGCAGAGTTGAACTTCTGGAGCAGACCTCCTAC	1871	0.1043384379794145	No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCC	1847	0.10300005074718255	No Hit
ATTTCTTCTGGGGGGGCACAGTGGCTCACACCTGTAATCCCAGCACTTTGGA	1814	0.10115976830286363	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.029779115917160523	0.0	0.0	0.0	0.0
12	0.049910690535315855	0.0	0.0	0.0	0.0
13	0.13997299803758972	0.0	0.0	0.0	0.0
14	0.463081982352249	0.0	0.0	0.0	0.0
15	0.8265656481725716	0.0	0.0	0.0	0.0
16	1.0504666788980388	0.0	0.0	0.0	0.0
17	1.206723388261117	0.0	0.0	0.0	0.0
18	1.4146195383344775	0.0	0.0	0.0	0.0
19	1.5957479437631992	0.0	0.0	0.0	0.0
20	1.7670615094888866	0.0	0.0	0.0	0.0
21	1.9308466470332695	0.0	0.0	0.0	0.0
22	2.1027736402403967	0.0	0.0	0.0	0.0
23	2.2812810373393306	0.0	0.0	0.0	0.0
24	2.47780089593872	0.0	0.0	0.0	0.0
25	2.6935600710014427	0.0	0.0	0.0	0.0
26	2.9256029573896543	0.0	0.0	0.0	0.0
27	3.161103344127798	0.0	0.0	0.0	0.0
28	3.3867331250282318	0.0	0.0	0.0	0.0
29	3.63645387610884	0.0	0.0	0.0	0.0
30	3.9179613239549567	0.0	0.0	0.0	0.0
31	4.180229455337739	0.0	0.0	0.0	0.0
32	4.410599357685661	0.0	0.0	0.0	0.0
33	4.61531683808247	0.0	0.0	0.0	0.0
34	4.809438752890777	0.0	0.0	0.0	0.0
35	4.999099376924978	0.0	0.0	0.0	0.0
36	5.180896975969815	0.0	0.0	0.0	0.0
37	5.366263607633938	0.0	0.0	0.0	0.0
38	5.553414755607703	0.0	0.0	0.0	0.0
39	5.748986589917594	0.0	0.0	0.0	0.0
40	5.952031086274114	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	70	0.0	46.000004	37
ATTTAGC	20	6.312404E-4	46.000004	24
ATTTACA	70	0.0	46.000004	35
CTAGATA	20	6.312404E-4	46.000004	46
ACTATTA	40	5.6152203E-9	46.000004	35
TGTCACG	20	6.312404E-4	46.000004	13
CTATGAA	75	0.0	46.000004	24
TTGGCTG	85	0.0	46.000004	17
TAGAGCG	20	6.312404E-4	46.000004	38
TAATAAG	35	1.0199983E-7	46.000004	17
TCCATTC	75	0.0	46.000004	18
ATCTCAC	75	0.0	46.000004	15
ATTAGTA	75	0.0	46.000004	20
GATAAAT	165	0.0	46.000004	33
TACTTAC	40	5.6152203E-9	46.000004	37
TTCAGAA	160	0.0	46.000004	46
TTTACGT	40	5.6152203E-9	46.000004	15
CCGATAT	80	0.0	46.000004	27
GCGCGGT	160	0.0	46.000004	13
GTTTACG	40	5.6152203E-9	46.000004	14
>>END_MODULE