SRR1528138_1.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR1528138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1675372
Sequences flagged as poor quality	0
Sequence length	59
%GC	38

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGATGATGGA	3277	0.1955983506946517	No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTCAATACCA	2856	0.17046960316872908	No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTCCAAAGTC	2812	0.16784332076696995	No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGGGACCCCA	2323	0.13865577316560143	No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCCTTGCCTC	2301	0.13734263196472188	No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG	2285	0.136387620182264	No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAG	2172	0.12964284946865534	No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTGCCCTGGC	2137	0.12755376119452874	No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2005	0.11967491398925134	No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCCAATAGTT	1993	0.11895865515240796	No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCTGTCTCTA	1818	0.10851321378177504	No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1798	0.10731944905370269	No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCGTGGTGGC	1768	0.1055288019615942	No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGTGAGCACC	1747	0.10427534899711825	No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGA	1725	0.10296220779623867	No Hit
ACACCTTCTGGGGCAGCCTGGCCAACGTGGTGAAACCCCGTCTCTACTAAAAATATAAA	1709	0.10200719601378082	No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGTCAAGAGA	1708	0.1019475077773772	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	75	0.0	53.000004	37
TTTAGTC	75	0.0	53.000004	28
ATCTCAG	45	7.6397555E-11	53.000004	44
CCGGCGT	195	0.0	53.000004	47
CCGGCCC	45	7.6397555E-11	53.000004	37
ATTCGAT	75	0.0	53.000004	18
TAAGTAC	75	0.0	53.000004	39
TGACGCG	130	0.0	53.000004	38
CATTCAA	180	0.0	53.000004	43
ACGATCT	45	7.6397555E-11	53.000004	41
GGGACTA	45	7.6397555E-11	53.000004	11
TATCTTT	75	0.0	53.000004	31
CTACGTT	65	0.0	53.000004	53
TAACATT	180	0.0	53.000004	40
ATCCCTA	65	0.0	53.000004	50
TACTGCA	75	0.0	53.000004	16
TATTCAA	75	0.0	53.000004	31
TCCACTA	150	0.0	53.000004	26
TTAACGG	75	0.0	53.000004	32
TTAGTCA	75	0.0	53.000004	29

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content