##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528138_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1675372
Sequences flagged as poor quality	0
Sequence length	59
%GC	38
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.694914323505465	31.0	31.0	33.0	27.0	34.0
2	31.3693985574547	31.0	31.0	34.0	28.0	34.0
3	31.529489570077573	31.0	31.0	34.0	30.0	34.0
4	35.275636097535354	37.0	35.0	37.0	33.0	37.0
5	34.99514197443911	37.0	35.0	37.0	32.0	37.0
6	35.19846159539493	37.0	35.0	37.0	32.0	37.0
7	35.33213817587974	37.0	35.0	37.0	33.0	37.0
8	35.26786886733215	37.0	35.0	37.0	32.0	37.0
9	37.24824397208501	39.0	37.0	39.0	34.0	39.0
10	36.72053012704044	39.0	35.0	39.0	32.0	39.0
11	37.13448535608808	39.0	37.0	39.0	34.0	39.0
12	37.223550351802466	39.0	37.0	39.0	35.0	39.0
13	36.876530107940205	39.0	37.0	39.0	32.0	39.0
14	36.513188712715746	39.0	36.0	40.0	30.0	40.0
15	35.201948582165635	38.0	33.0	40.0	27.0	40.0
16	35.47633540491306	38.0	34.0	40.0	27.0	40.0
17	35.42814312284078	38.0	34.0	40.0	27.0	40.0
18	35.81406994983801	38.0	34.0	40.0	29.0	40.0
19	36.176484983633486	38.0	35.0	40.0	30.0	40.0
20	36.21601709948597	38.0	35.0	40.0	30.0	40.0
21	36.22353901103755	38.0	35.0	40.0	30.0	40.0
22	36.263904374670226	38.0	35.0	40.0	30.0	40.0
23	36.289858610505604	38.0	35.0	40.0	30.0	40.0
24	36.33778945810244	39.0	35.0	40.0	30.0	40.0
25	36.36261916756398	39.0	35.0	40.0	30.0	40.0
26	35.9741239557543	39.0	35.0	40.0	29.0	40.0
27	35.4725971306671	38.0	34.0	40.0	27.0	40.0
28	34.87583175557428	37.0	33.0	40.0	26.0	40.0
29	34.48398982434946	36.0	33.0	40.0	25.0	40.0
30	34.161527111590736	35.0	33.0	40.0	25.0	40.0
31	33.94709771919311	35.0	33.0	40.0	24.0	40.0
32	33.79103029058621	35.0	33.0	39.0	23.0	40.0
33	33.4914228004288	35.0	33.0	39.0	21.0	40.0
34	33.32333714542203	35.0	32.0	39.0	21.0	40.0
35	33.22992266792092	35.0	32.0	39.0	21.0	40.0
36	32.87415153171952	35.0	31.0	39.0	19.0	40.0
37	32.72634674567797	35.0	31.0	39.0	18.0	40.0
38	32.42333165410428	35.0	31.0	39.0	16.0	40.0
39	31.680854162538232	35.0	30.0	39.0	14.0	40.0
40	31.051312186189097	35.0	28.0	38.0	10.0	40.0
41	30.21363374820637	35.0	25.0	38.0	9.0	40.0
42	29.588799383062387	34.0	24.0	38.0	9.0	40.0
43	29.008143862974908	34.0	22.0	37.0	8.0	40.0
44	28.505136769624894	33.0	21.0	37.0	8.0	40.0
45	28.17824518972503	33.0	20.0	37.0	8.0	40.0
46	27.763355839777674	33.0	19.0	36.0	8.0	40.0
47	27.267101873494365	33.0	17.0	36.0	8.0	40.0
48	26.89094601079641	33.0	15.0	35.0	8.0	39.0
49	26.515048001279716	33.0	14.0	35.0	8.0	39.0
50	25.87915101840069	32.0	12.0	35.0	8.0	39.0
51	25.533994838161316	31.0	10.0	35.0	8.0	38.0
52	24.94633788794369	31.0	10.0	35.0	8.0	38.0
53	24.73153066900963	31.0	10.0	35.0	8.0	38.0
54	24.321292226442843	29.0	10.0	35.0	8.0	38.0
55	23.900871567627966	28.0	10.0	35.0	8.0	38.0
56	23.245809288922104	27.0	9.0	35.0	8.0	37.0
57	22.579183608177765	25.0	9.0	35.0	8.0	37.0
58	21.94353970342109	23.0	8.0	34.0	8.0	37.0
59	21.141844915636646	20.0	8.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	5.0
13	5.0
14	39.0
15	151.0
16	627.0
17	1925.0
18	4377.0
19	8487.0
20	14294.0
21	21561.0
22	30663.0
23	41410.0
24	54006.0
25	67687.0
26	82823.0
27	94243.0
28	88580.0
29	74775.0
30	73490.0
31	83709.0
32	97538.0
33	113410.0
34	134233.0
35	144688.0
36	158492.0
37	151548.0
38	122489.0
39	10116.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.349190508137895	24.993553670468412	35.9320198737952	5.725235947598503
2	24.012279063992953	21.494211434833577	34.16441244093849	20.32909706023498
3	21.702941197537022	20.85310008762233	36.80054340170422	20.643415313136426
4	30.580671039028946	20.909326406314538	38.23879114608577	10.271211408570753
5	0.0	0.0	0.0	100.0
6	0.0	0.0	100.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	100.0	0.0	0.0	0.0
11	98.66728105758004	0.7472967197732802	0.20079122726176635	0.3846309953849056
12	97.87092060748299	1.2615705646268411	0.3700670657024231	0.497441762187741
13	90.07724851555356	6.663296270917743	1.9367042065881488	1.3227510069405481
14	38.601635935183346	43.365115329610376	11.125827577397736	6.907421157808534
15	24.349040093782158	53.21641999508169	14.228481793894133	8.206058117242021
16	33.288368195242604	39.85735705264264	16.092784169724695	10.76149058239006
17	17.06283738775627	39.228720546839746	27.35147776135688	16.3569643040471
18	16.684234904248132	44.403332513614885	26.013267501187798	12.899165080949187
19	20.885212358807475	44.06340800729629	23.652657439661162	11.398722194235072
20	17.183586690000787	44.665065430244745	25.471358002879363	12.679989876875105
21	19.546226151565143	45.1808314810084	18.307814622662907	16.96512774476355
22	16.125075505619048	43.60774801059108	19.388112013331966	20.8790644704579
23	11.591634574291561	41.8322020422927	31.26368352819553	15.312479855220214
24	12.50725212072304	42.65536251053497	27.58330687154853	17.254078497193458
25	16.319957597476858	38.92860809420236	21.83986601184692	22.91156829647386
26	17.84081386104101	40.98355469710608	23.952650515825738	17.222980926027176
27	26.82437094567654	44.93067808224084	15.208383570932307	13.036567401150311
28	28.376324780406982	44.375935613105625	12.961181158572543	14.286558447914851
29	30.454430419035294	45.60700548893022	11.062140229155078	12.876423862879408
30	30.60317350415311	48.51746358420697	11.31945621629107	9.559906695348854
31	21.365822038329398	54.89574852629744	12.969119694014225	10.769309741358935
32	17.613461368579635	60.337226598033155	11.934006298302705	10.115305735084506
33	16.05458369842638	59.38758675685162	13.800278385934586	10.757551158787422
34	13.23640361662962	60.5096659130032	15.578689389580344	10.675241080786835
35	14.773733833441169	59.38328920383055	13.913924788047073	11.929052174681205
36	14.255460876748566	59.18202047067756	14.640629066261104	11.921889586312771
37	16.18595750675074	58.721824168005675	15.642555802532213	9.449662522711375
38	16.57679607872162	59.96584639112985	13.523444345494612	9.933913184653916
39	15.174898470309877	60.424371423182436	13.107477025997808	11.293253080509881
40	19.165892709201298	57.75099500290085	12.599768887148647	10.483343400749206
41	19.572548663819138	56.60336928156852	12.072483006759096	11.751599047853253
42	17.64969212807663	58.02884374335968	13.011080524205967	11.310383604357718
43	18.839039926655097	55.789579866441606	13.745484584916065	11.625895621987237
44	18.84208402671168	53.70514727475451	13.200471298314643	14.252297400219174
45	20.00343804241685	52.83214712911521	12.852787321263575	14.311627507204369
46	21.24787808319585	54.95191515675325	10.692073163452655	13.108133596598249
47	21.146646834255318	54.70576086982473	12.225463956661565	11.922128339258386
48	21.153331916732522	55.61391738670576	12.228328992008938	11.00442170455278
49	21.701866809281757	54.63801472150662	11.18265077845398	12.477467690757635
50	19.555179387025685	54.477632430290114	13.799681503570552	12.167506679113654
51	19.857082486755182	53.34427219745823	13.34282774213727	13.455817573649314
52	19.176755968226757	52.416000744909184	12.86114367436008	15.54609961250397
53	17.061404870082587	53.40551232800834	14.17052451634622	15.362558285562846
54	17.76835234204702	52.516814176194906	14.48937907521434	15.22545440654374
55	19.012673006353214	50.112989831512046	14.662057143130003	16.212280019004734
56	18.36463782371915	48.243673643823584	16.284681849762322	17.107006682694948
57	18.929646669515783	45.69158371991414	17.003925098425903	18.37484451214417
58	21.353526261630254	43.16295127291133	17.48214724849168	18.00137521696674
59	23.437839476844545	40.25386600707186	17.99063133441409	18.317663181669506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	2.0
6	12.5
7	23.0
8	266.5
9	3640.0
10	6770.0
11	11058.0
12	15346.0
13	17868.0
14	19104.5
15	17819.0
16	14734.0
17	11649.0
18	10869.0
19	10384.0
20	10679.0
21	12648.0
22	14617.0
23	19106.0
24	23595.0
25	33280.0
26	55416.5
27	67868.0
28	81019.5
29	94171.0
30	102069.5
31	109955.5
32	109943.0
33	108371.0
34	106799.0
35	102603.0
36	92262.5
37	86118.0
38	81564.5
39	77011.0
40	75103.0
41	73195.0
42	71225.5
43	71282.0
44	73308.0
45	69540.0
46	65772.0
47	67647.0
48	73041.5
49	76561.0
50	78017.5
51	79474.0
52	75492.5
53	62100.0
54	52689.0
55	46829.0
56	40969.0
57	35845.0
58	23186.5
59	15652.0
60	11675.0
61	7698.0
62	9425.0
63	11152.0
64	10125.0
65	5552.5
66	2007.0
67	1371.0
68	735.0
69	537.0
70	517.5
71	696.0
72	417.5
73	139.0
74	99.0
75	40.0
76	21.0
77	19.5
78	18.0
79	16.5
80	15.0
81	10.0
82	6.5
83	8.0
84	7.5
85	7.0
86	4.0
87	3.0
88	5.0
89	2.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	1675372.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.928908064795095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.2167077876952	52.497964796837735
2	3.620415353748535	4.122125856598459
3	1.1262435979267313	1.9234745473480481
4	0.570131855660989	1.2982793598294189
5	0.3850095413782939	1.0959086392596906
6	0.26680488794579865	0.9113346562262598
7	0.19696671604142618	0.7849170048542866
8	0.15415547893313541	0.7020722470295138
9	0.14195792864279422	0.7273358881896936
>10	1.1662206736118201	13.578947480386605
>50	0.07848807104642906	2.9397157439546904
>100	0.05206766641912177	7.113825371502724
>500	0.01979872163824337	7.758132367810303
>1k	0.005031719311376768	4.545966040172474
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGTCTTCTGAGCTACTGCGCTTGGCCCCCTCCCCCCACCACAGCCCTCAGATGATGGA	3277	0.1955983506946517	No Hit
TTCACTTCTGGGTGGAGGCTGAGGTGGGCACATTGCTTAAGCCCAGGAGTTCAATACCA	2856	0.17046960316872908	No Hit
GGCGCTTCTGGAAAGTTCCTTTCAGCTCTGACACTCTGCGTGGCCTGATTTCCAAAGTC	2812	0.16784332076696995	No Hit
ATTACTTCTGGGGAGTGGCCTCAGAGAGAGGGGACCCACAGCTGGAGGCTGGGACCCCA	2323	0.13865577316560143	No Hit
TTGGCTTCTGCCGATTTCACCCCCGCGGTGCATGCCTCTCTGGACAAATTCCTTGCCTC	2301	0.13734263196472188	No Hit
CTAGCTTCTGGGGACTTCTGATTCTGACAGACTCAGGAAGAAACCATGGTGCTCTCTGG	2285	0.136387620182264	No Hit
ATATCTTCTGGGGCGGTGCCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCAG	2172	0.12964284946865534	No Hit
CTATCTTCTGGGGGTGAAATCCTTGCCCAGGTGGTGGCTGGAGTGGCCGCTGCCCTGGC	2137	0.12755376119452874	No Hit
GCTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2005	0.11967491398925134	No Hit
CCTGCTTCTGGGGACAGAATACAAAGATCATGAAGAAACGGAAAAGCATTCCAATAGTT	1993	0.11895865515240796	No Hit
CTTTCTTCTGGGGAGGAGTTCGAGACCAGCCTGACCAACATGGTGCAACCCTGTCTCTA	1818	0.10851321378177504	No Hit
ACTGCTTCTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1798	0.10731944905370269	No Hit
ACAACTTCTGGGGAGATTACTGTCATCCAAAAGCAGGGAACTAGGCCCGGCGTGGTGGC	1768	0.1055288019615942	No Hit
TAAACTTCTGGGGCGCGGTGCATGCCTCTCTGGATAAATTCCTTGCCTCTGTGAGCACC	1747	0.10427534899711825	No Hit
AGTACTTCTGGGGTTCACCGCGTGCGCCGGTAAGGCCAAGCTGTTGGTACAACCGTTGA	1725	0.10296220779623867	No Hit
ACACCTTCTGGGGCAGCCTGGCCAACGTGGTGAAACCCCGTCTCTACTAAAAATATAAA	1709	0.10200719601378082	No Hit
GTTGCTTCTGGGGCCAACACTTTGGGAGGCCAAGGTGGACAGATCATGAAGTCAAGAGA	1708	0.1019475077773772	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.028590665237332367	0.0	0.0	0.0	0.0
12	0.04769090088648969	0.0	0.0	0.0	0.0
13	0.12821033179496852	0.0	0.0	0.0	0.0
14	0.4253383726121721	0.0	0.0	0.0	0.0
15	0.742820102043009	0.0	0.0	0.0	0.0
16	0.9412237998486306	0.0	0.0	0.0	0.0
17	1.072239478754569	0.0	0.0	0.0	0.0
18	1.2487375938000636	0.0	0.0	0.0	0.0
19	1.4073889261608765	0.0	0.0	0.0	0.0
20	1.556907958351936	0.0	0.0	0.0	0.0
21	1.7015325551578993	0.0	0.0	0.0	0.0
22	1.857438228644146	0.0	0.0	0.0	0.0
23	2.0117920079838987	0.0	0.0	0.0	0.0
24	2.183156934698682	0.0	0.0	0.0	0.0
25	2.3592969203257543	0.0	0.0	0.0	0.0
26	2.555193712202424	0.0	0.0	0.0	0.0
27	2.7466138863488228	0.0	0.0	0.0	0.0
28	2.9369596722399565	0.0	0.0	0.0	0.0
29	3.1515985703473617	0.0	0.0	0.0	0.0
30	3.399424127895178	0.0	0.0	0.0	0.0
31	3.631850120450861	0.0	0.0	0.0	0.0
32	3.8257772005262116	0.0	0.0	0.0	0.0
33	4.008542580394086	0.0	0.0	0.0	0.0
34	4.1798478188724655	0.0	0.0	0.0	0.0
35	4.3454826748925015	0.0	0.0	0.0	0.0
36	4.504850266090158	0.0	0.0	0.0	0.0
37	4.668515410308875	0.0	0.0	0.0	0.0
38	4.835821536948212	0.0	0.0	0.0	0.0
39	5.011543704920459	0.0	0.0	0.0	0.0
40	5.197472561317725	0.0	0.0	0.0	0.0
41	5.386982711899208	0.0	0.0	0.0	0.0
42	5.5963093569666915	0.0	0.0	0.0	0.0
43	5.801696578431536	0.0	0.0	0.0	0.0
44	6.011142599971827	0.0	0.0	0.0	0.0
45	6.222080827422208	0.0	0.0	0.0	0.0
46	6.4195892016817755	0.0	0.0	0.0	0.0
47	6.615127864140024	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCAG	75	0.0	53.000004	37
TTTAGTC	75	0.0	53.000004	28
ATCTCAG	45	7.6397555E-11	53.000004	44
CCGGCGT	195	0.0	53.000004	47
CCGGCCC	45	7.6397555E-11	53.000004	37
ATTCGAT	75	0.0	53.000004	18
TAAGTAC	75	0.0	53.000004	39
TGACGCG	130	0.0	53.000004	38
CATTCAA	180	0.0	53.000004	43
ACGATCT	45	7.6397555E-11	53.000004	41
GGGACTA	45	7.6397555E-11	53.000004	11
TATCTTT	75	0.0	53.000004	31
CTACGTT	65	0.0	53.000004	53
TAACATT	180	0.0	53.000004	40
ATCCCTA	65	0.0	53.000004	50
TACTGCA	75	0.0	53.000004	16
TATTCAA	75	0.0	53.000004	31
TCCACTA	150	0.0	53.000004	26
TTAACGG	75	0.0	53.000004	32
TTAGTCA	75	0.0	53.000004	29
>>END_MODULE