FastQCFastQC Report
Fri 17 Jun 2016
SRR1528134_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528134_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2317690
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGT62720.27061427542078537No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGC31450.13569545538877073No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTA29500.12728190569057984No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTT28290.12206119023683062No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGG27130.11705620682662479No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG24540.10588128697107896No Hit
AGTAGATCTTGGGCGTCCACCACCACAGCTGGCTAGGTTTTGTATTTTTAAT24350.10506150520561422No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTTT1100.046.00000426
CTATGTG1400.046.00000423
AATCCCC551.8189894E-1246.00000436
TACCCGT351.0201802E-746.00000438
CTGTATA351.0201802E-746.00000436
CTCAAGT351.0201802E-746.00000445
CATGCGT1450.046.00000417
CATGCAA1450.046.00000430
GACACAA700.046.00000427
TTTCGCA1100.046.00000418
CGTGACG2800.046.00000423
CTATACA551.8189894E-1246.00000440
ATCAGGC700.046.00000420
TAGAAGC551.8189894E-1246.00000436
GACAAGT1400.046.00000433
CCGTGCA351.0201802E-746.00000438
TATATTA700.046.00000434
GTTAGCT700.046.00000413
CAATTAG700.046.00000420
CTAAGGT2900.046.00000415
Produced by FastQC (version 0.11.5)