##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528134_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2317690
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.517774163067536	31.0	31.0	33.0	27.0	34.0
2	31.481874625165574	31.0	31.0	34.0	28.0	34.0
3	31.75582584383589	31.0	31.0	34.0	30.0	34.0
4	35.47312841665624	37.0	35.0	37.0	33.0	37.0
5	35.39256716817176	37.0	35.0	37.0	33.0	37.0
6	34.94846636090245	35.0	35.0	37.0	32.0	37.0
7	35.323098861366276	37.0	35.0	37.0	33.0	37.0
8	35.3318152988536	37.0	35.0	37.0	32.0	37.0
9	37.48182198654695	39.0	37.0	39.0	35.0	39.0
10	37.79879276348433	39.0	37.0	39.0	35.0	39.0
11	37.64281547575388	39.0	37.0	39.0	35.0	39.0
12	37.57825723025944	39.0	37.0	39.0	35.0	39.0
13	37.50557063282837	39.0	37.0	39.0	35.0	39.0
14	37.711407910462576	40.0	37.0	40.0	33.0	40.0
15	36.97180727362158	39.0	36.0	40.0	31.0	40.0
16	36.59417394043207	38.0	35.0	40.0	31.0	40.0
17	36.2793043935988	38.0	35.0	40.0	30.0	40.0
18	36.44172171429311	39.0	35.0	40.0	31.0	40.0
19	36.65760433880286	39.0	35.0	40.0	31.0	40.0
20	36.80741557326476	39.0	35.0	40.0	31.0	40.0
21	36.83116680833071	39.0	35.0	40.0	31.0	40.0
22	36.873866220245155	39.0	35.0	40.0	31.0	40.0
23	36.77831159473441	39.0	35.0	40.0	31.0	40.0
24	36.7985131747559	39.0	35.0	40.0	31.0	40.0
25	36.88428348916378	39.0	35.0	40.0	31.0	40.0
26	36.673176309169904	39.0	35.0	40.0	31.0	40.0
27	36.43703601430735	39.0	35.0	40.0	30.0	40.0
28	36.05051452092385	38.0	35.0	40.0	30.0	40.0
29	35.81471551415418	38.0	35.0	40.0	30.0	40.0
30	35.58756865672286	37.0	35.0	40.0	30.0	40.0
31	35.45826189007158	37.0	35.0	40.0	29.0	40.0
32	35.38020270182811	37.0	34.0	40.0	29.0	40.0
33	35.165409524138255	37.0	34.0	40.0	27.0	40.0
34	34.88851313160949	37.0	34.0	40.0	25.0	40.0
35	34.75658824087777	37.0	34.0	40.0	25.0	40.0
36	34.51319503471129	36.0	33.0	40.0	24.0	40.0
37	34.30366097277893	36.0	33.0	40.0	23.0	40.0
38	33.93496714400977	35.0	33.0	40.0	21.0	40.0
39	33.460650043793606	35.0	33.0	40.0	20.0	40.0
40	32.94927578752983	35.0	33.0	40.0	17.0	40.0
41	32.3148686839051	35.0	32.0	40.0	15.0	40.0
42	31.662249481164434	35.0	31.0	39.0	10.0	40.0
43	31.022449939379296	35.0	29.0	39.0	9.0	40.0
44	30.42691127803977	35.0	26.0	39.0	9.0	40.0
45	30.02282962777593	35.0	24.0	39.0	8.0	40.0
46	29.571433194258077	35.0	23.0	39.0	8.0	40.0
47	29.053212034396317	35.0	20.0	38.0	8.0	40.0
48	28.6184938451648	35.0	20.0	38.0	8.0	40.0
49	28.295405338936614	34.0	18.0	38.0	8.0	40.0
50	27.923534208630144	34.0	17.0	37.0	8.0	40.0
51	27.524458836168773	34.0	15.0	37.0	8.0	40.0
52	26.69075243022147	33.0	10.0	36.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	2.0
13	7.0
14	11.0
15	43.0
16	189.0
17	575.0
18	1539.0
19	3137.0
20	5751.0
21	9612.0
22	14312.0
23	20206.0
24	28186.0
25	37865.0
26	50123.0
27	65165.0
28	83164.0
29	104387.0
30	124507.0
31	128157.0
32	115485.0
33	116929.0
34	142494.0
35	188803.0
36	264390.0
37	327825.0
38	404121.0
39	80703.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.615742398681444	23.558068594160563	38.992315624608985	6.833873382549004
2	25.85475192972313	21.56625778253347	34.247418766099	18.331571521644396
3	18.489401084700717	23.053169319451698	36.45957828700128	21.997851308846307
4	20.85196898636142	20.833372884207982	34.51475391445793	23.799904214972667
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.44336818124944	0.38767048224741013	0.11149032010320621	0.05747101639994995
12	98.73589651765336	0.9339903093166041	0.15847675918694906	0.17163641384309378
13	94.28853729359837	3.6787059529100095	0.9171200635115137	1.1156366899801096
14	45.53283657434773	34.69989515422684	10.820170083143129	8.947098188282299
15	29.329504808667252	41.178155836198975	18.87819337357455	10.614145981559226
16	35.034064089675496	33.19982396265247	18.397801259012205	13.368310688659829
17	20.197049648572502	36.74123804305149	21.62044967187156	21.44126263650445
18	17.46609770935716	46.40935586726439	20.63023959200756	15.49430683137089
19	22.581061315361417	44.933317225340744	21.445361545331775	11.04025991396606
20	19.129995814798356	43.527866108064494	22.70454633708563	14.637591740051517
21	23.815221190064246	44.391743503229506	14.837014441102994	16.956020865603254
22	25.13964335178561	45.791887612234596	17.01841057259599	12.0500584633838
23	16.646143358257575	47.511185706457724	20.494328404575246	15.348342530709456
24	18.038132796016722	48.12882654712235	18.6326471616135	15.200393495247422
25	19.46369013975122	45.205916235562135	19.174134590907325	16.156259033779325
26	19.101389745824505	46.26567832626451	17.59601154597897	17.03692038193201
27	19.954394245994937	49.34892069258615	15.942209700175605	14.754475361243308
28	20.862280978042794	48.19919833972619	15.707795261661396	15.230725420569618
29	22.721934339795226	48.02562033749121	14.14882922219969	15.103616100513872
30	24.262217984286078	48.74072891542872	13.938835651014589	13.05821744927061
31	19.404752145455173	52.009543985606356	14.474886632811119	14.110817236127351
32	19.540490747252655	54.564846894968696	15.727858341710926	10.166804016067722
33	21.783111632703253	53.76581855209282	13.424961923294315	11.026107891909618
34	18.52340045476315	56.09904689583163	13.993674736483309	11.383877912921918
35	19.838718724246988	53.31364418882595	15.673148695468333	11.174488391458738
36	17.651929291665407	52.65708528750609	17.19802907205019	12.49295634877831
37	17.553253454948678	53.822167761866346	17.631175869076536	10.993402914108444
38	16.887374929347757	53.076295794519545	18.23794381474658	11.798385461386122
39	19.800534152539814	52.11943788858734	16.095552036726225	11.98447592214662
40	21.09773093036601	51.044747140471756	15.317104530804379	12.540417398357848
41	22.553490760196578	51.50576651752391	15.416341270834321	10.52440145144519
42	19.77913353382031	51.55288239583379	15.637725493918515	13.030258576427393
43	20.955175195992563	47.54867993562556	17.50984816778775	13.986296700594124
44	20.58868960042111	47.50492947719497	15.899149584284352	16.007231338099572
45	21.10748201873417	46.3472250387239	20.063813538480126	12.481479404061803
46	23.23843999844673	45.89323852629126	17.326087613097524	13.542233862164482
47	25.557128002450717	46.14560187082828	15.079756136497979	13.217513990223024
48	26.178349995038165	46.23366369100268	14.67387786977551	12.914108444183647
49	24.647687999689346	47.24225414097658	13.916442664894788	14.19361519443929
50	22.92955485850135	49.0352031548654	14.346482920494113	13.68875906613913
51	22.53088204203323	49.090473704421214	14.333021240977006	14.045623012568548
52	22.631887784820233	48.14543791447519	15.475710729217454	13.746963571487129
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	7.5
4	15.0
5	27.0
6	39.0
7	376.5
8	714.0
9	3768.5
10	6823.0
11	17767.0
12	28711.0
13	31755.5
14	36216.5
15	37633.0
16	32828.0
17	28023.0
18	30846.0
19	33669.0
20	38212.0
21	42755.0
22	53089.0
23	63423.0
24	78982.0
25	94541.0
26	107546.5
27	120552.0
28	125878.5
29	131205.0
30	122819.0
31	114433.0
32	108957.0
33	103481.0
34	99820.5
35	96160.0
36	91919.0
37	87678.0
38	87479.5
39	90631.0
40	93981.0
41	100878.0
42	107775.0
43	113514.5
44	119254.0
45	126154.0
46	133054.0
47	133733.0
48	134412.0
49	136952.0
50	139492.0
51	126937.5
52	114383.0
53	110204.0
54	106025.0
55	90091.0
56	74157.0
57	62301.5
58	50446.0
59	44296.5
60	38147.0
61	37692.5
62	37238.0
63	31850.5
64	18719.0
65	10975.0
66	9777.0
67	8579.0
68	7908.5
69	7238.0
70	5419.5
71	3601.0
72	2012.0
73	423.0
74	239.0
75	55.0
76	45.0
77	35.0
78	23.0
79	11.0
80	8.0
81	5.0
82	4.0
83	3.0
84	2.0
85	1.0
86	1.0
87	1.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2317690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.66278295074795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.50138313533414	35.173869673715814
2	5.967798960078285	4.853346276147253
3	2.0210418660071117	2.4654356019546535
4	0.9755337669036906	1.5867167129892126
5	0.6187376644351491	1.2579797676189592
6	0.4148695911022048	1.0121851281512704
7	0.3457453938672229	0.9841278934930621
8	0.25824030821934146	0.8400615681805865
9	0.21843789543066983	0.7994063457103953
>10	2.3184889456289564	20.22386799257767
>50	0.20361242329071	5.582993029180588
>100	0.12021150419122288	10.312092736817254
>500	0.02132156036430268	6.1171291160787336
>1k	0.01446820167577682	8.513350083379828
>5k	1.0878347124644225E-4	0.27743807400477133
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGT	6272	0.27061427542078537	No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGC	3145	0.13569545538877073	No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTA	2950	0.12728190569057984	No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTT	2829	0.12206119023683062	No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGG	2713	0.11705620682662479	No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG	2454	0.10588128697107896	No Hit
AGTAGATCTTGGGCGTCCACCACCACAGCTGGCTAGGTTTTGTATTTTTAAT	2435	0.10506150520561422	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.02472289219006856	0.0	0.0	0.0	0.0
12	0.035423201549819004	0.0	0.0	0.0	0.0
13	0.0830999831729006	0.0	0.0	0.0	0.0
14	0.5045541034392003	0.0	0.0	0.0	0.0
15	0.9776544749297792	0.0	0.0	0.0	0.0
16	1.2388628332520744	0.0	0.0	0.0	0.0
17	1.4375088989467961	0.0	0.0	0.0	0.0
18	1.6938848594937201	0.0	0.0	0.0	0.0
19	1.9373600438367513	0.0	0.0	0.0	0.0
20	2.1962816424974867	0.0	0.0	0.0	0.0
21	2.4237926556183096	0.0	0.0	0.0	0.0
22	2.6384460389439486	0.0	0.0	0.0	0.0
23	2.837955032812844	0.0	0.0	0.0	0.0
24	3.0589509382186573	0.0	0.0	0.0	0.0
25	3.3082077413286504	0.0	0.0	0.0	0.0
26	3.593189770849423	0.0	0.0	0.0	0.0
27	3.8649689993053427	0.0	0.0	0.0	0.0
28	4.16116909509037	0.0	0.0	0.0	0.0
29	4.478252052690395	0.0	0.0	0.0	0.0
30	4.825235471525527	0.0	0.0	0.0	0.0
31	5.13425005069703	0.0	0.0	0.0	0.0
32	5.397788315089594	0.0	0.0	0.0	0.0
33	5.629225651402906	0.0	0.0	0.0	0.0
34	5.850696167304514	0.0	0.0	0.0	0.0
35	6.044509835223865	0.0	0.0	0.0	0.0
36	6.238970699273846	0.0	0.0	0.0	0.0
37	6.4300661434445505	0.0	0.0	0.0	0.0
38	6.6198671953539945	0.0	0.0	0.0	0.0
39	6.8204979958493155	0.0	0.0	0.0	0.0
40	7.017547644421817	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTT	110	0.0	46.000004	26
CTATGTG	140	0.0	46.000004	23
AATCCCC	55	1.8189894E-12	46.000004	36
TACCCGT	35	1.0201802E-7	46.000004	38
CTGTATA	35	1.0201802E-7	46.000004	36
CTCAAGT	35	1.0201802E-7	46.000004	45
CATGCGT	145	0.0	46.000004	17
CATGCAA	145	0.0	46.000004	30
GACACAA	70	0.0	46.000004	27
TTTCGCA	110	0.0	46.000004	18
CGTGACG	280	0.0	46.000004	23
CTATACA	55	1.8189894E-12	46.000004	40
ATCAGGC	70	0.0	46.000004	20
TAGAAGC	55	1.8189894E-12	46.000004	36
GACAAGT	140	0.0	46.000004	33
CCGTGCA	35	1.0201802E-7	46.000004	38
TATATTA	70	0.0	46.000004	34
GTTAGCT	70	0.0	46.000004	13
CAATTAG	70	0.0	46.000004	20
CTAAGGT	290	0.0	46.000004	15
>>END_MODULE