FastQCFastQC Report
Fri 17 Jun 2016
SRR1528133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2321062
Sequences flagged as poor quality0
Sequence length52
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGT62880.2709104711550144No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGC30520.1314915327552646No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTA29500.12709699266973482No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTT28360.1221854478682603No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGG27350.11783399150905921No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG24950.10749389719016554No Hit
AGTAGATCTTGGGCGTCCACCACCACAGCTGGCTAGGTTTTGTATTTTTAAT24920.10736464601117937No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTCC351.0201802E-746.00000437
CTAGATT1550.046.00000436
CTATGTG1550.046.00000423
CTCACCA351.0201802E-746.00000415
CATATTA551.8189894E-1246.00000419
CTACTTC551.8189894E-1246.00000439
CTGTATA351.0201802E-746.00000436
TTTACAT1550.046.00000438
CAACGAC351.0201802E-746.00000420
ACACCTA551.8189894E-1246.00000435
TAGGTTT3100.046.00000434
CACATCG1550.046.00000438
AGCCATA551.8189894E-1246.00000422
ACACATT351.0201802E-746.00000428
TTGTCAC351.0201802E-746.00000423
CCTACTT551.8189894E-1246.00000438
TATATTA700.046.00000434
CCTGTGC1400.046.00000414
CAACAAC351.0201802E-746.00000432
ACTTACG1550.046.00000442