##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528133_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2321062
Sequences flagged as poor quality	0
Sequence length	52
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.555586192872056	31.0	31.0	33.0	27.0	34.0
2	31.510760591487863	31.0	31.0	34.0	28.0	34.0
3	31.77616970162796	31.0	31.0	34.0	30.0	34.0
4	35.50393828342371	37.0	35.0	37.0	33.0	37.0
5	35.58859651314786	37.0	35.0	37.0	33.0	37.0
6	35.02225188297426	36.0	35.0	37.0	32.0	37.0
7	35.320202562447705	37.0	35.0	37.0	33.0	37.0
8	35.33256888441584	37.0	35.0	37.0	32.0	37.0
9	37.48526881229368	39.0	37.0	39.0	35.0	39.0
10	37.78214972284239	39.0	37.0	39.0	35.0	39.0
11	37.62981471412655	39.0	37.0	39.0	35.0	39.0
12	37.5900441263525	39.0	37.0	39.0	35.0	39.0
13	37.48999768209552	39.0	37.0	39.0	35.0	39.0
14	37.66173243110266	40.0	37.0	40.0	33.0	40.0
15	36.97272713955939	39.0	36.0	40.0	31.0	40.0
16	36.57978589111364	38.0	35.0	40.0	31.0	40.0
17	36.284594724311546	38.0	35.0	40.0	30.0	40.0
18	36.369153430627875	39.0	35.0	40.0	30.0	40.0
19	36.66611361523303	39.0	35.0	40.0	31.0	40.0
20	36.81699325567348	39.0	35.0	40.0	31.0	40.0
21	36.881866576592955	39.0	35.0	40.0	31.0	40.0
22	36.80542182845611	39.0	35.0	40.0	31.0	40.0
23	36.7975698193327	39.0	35.0	40.0	31.0	40.0
24	36.909087305724704	39.0	35.0	40.0	31.0	40.0
25	36.919454973628454	39.0	35.0	40.0	31.0	40.0
26	36.590088071753364	39.0	35.0	40.0	31.0	40.0
27	36.319540365574035	39.0	35.0	40.0	30.0	40.0
28	35.98208233989441	38.0	35.0	40.0	30.0	40.0
29	35.744150737894984	38.0	35.0	40.0	30.0	40.0
30	35.39967135733556	37.0	34.0	40.0	29.0	40.0
31	35.331684806351575	37.0	34.0	40.0	29.0	40.0
32	35.33926194130101	37.0	34.0	40.0	29.0	40.0
33	35.21847542202664	37.0	34.0	40.0	28.0	40.0
34	34.99397129417482	37.0	34.0	40.0	26.0	40.0
35	34.67876945984209	37.0	33.0	40.0	25.0	40.0
36	34.488381611520936	36.0	33.0	40.0	24.0	40.0
37	34.273542455996434	36.0	33.0	40.0	23.0	40.0
38	33.95389093440847	35.0	33.0	40.0	22.0	40.0
39	33.486874542773954	35.0	33.0	40.0	20.0	40.0
40	32.90626230578933	35.0	33.0	40.0	17.0	40.0
41	32.24862325952517	35.0	31.0	40.0	15.0	40.0
42	31.596745799982937	35.0	31.0	39.0	10.0	40.0
43	30.994175080200357	35.0	29.0	39.0	9.0	40.0
44	30.329677104704658	35.0	25.0	39.0	8.0	40.0
45	29.925995514122416	35.0	23.0	39.0	8.0	40.0
46	29.46548691934985	35.0	23.0	38.0	8.0	40.0
47	29.076381845896403	35.0	20.0	38.0	8.0	40.0
48	28.63240964696333	35.0	20.0	38.0	8.0	40.0
49	28.13537811570738	34.0	18.0	38.0	8.0	40.0
50	27.768243157658002	34.0	17.0	37.0	8.0	40.0
51	27.37061009141505	33.0	15.0	37.0	8.0	40.0
52	26.38560882906187	33.0	10.0	35.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	6.0
14	13.0
15	39.0
16	182.0
17	561.0
18	1474.0
19	3200.0
20	5941.0
21	9541.0
22	14372.0
23	20460.0
24	28271.0
25	38423.0
26	51158.0
27	66163.0
28	83889.0
29	106012.0
30	125205.0
31	127383.0
32	116304.0
33	117935.0
34	143332.0
35	188197.0
36	264616.0
37	329971.0
38	405545.0
39	72868.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.632787922080496	23.5706327534551	38.97767487469098	6.818904449773424
2	25.807884494253063	21.516400682101555	34.37650523768861	18.299209585956774
3	18.45594818234067	23.135917954798277	36.449521813721475	21.958612049139575
4	20.825251544336172	20.780616803859612	34.51820761358378	23.875924038220436
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.44133332069543	0.3905108954435513	0.11257777689695495	0.05557800696405353
12	98.78215230786597	0.9060938484193873	0.14885427446574026	0.16289956924890417
13	94.2822294277361	3.6991687425842135	0.904585917997882	1.1140159116818078
14	45.42756720845888	34.80613615663864	10.858994718796827	8.907301916105645
15	29.262337671290123	41.28347282407795	18.826985233483637	10.627204271148294
16	35.052747406144256	33.221215116183885	18.35832045847978	13.367717019192076
17	20.162882335758372	36.78346377649541	21.583266625363734	21.470387262382477
18	17.4826868045748	46.407161893995074	20.538787847976486	15.571363453453635
19	22.630373510057034	44.97424024002806	21.391673294379903	11.00371295553501
20	19.133913699849465	43.54545462378859	22.647951670399152	14.672680005962787
21	23.900352511048823	44.40260535909855	14.782500424374703	16.914541705477923
22	25.151676258540274	45.71058420671228	17.09889697043853	12.038842564308924
23	16.64862894657704	47.50114387293402	20.497039717163954	15.353187463324977
24	18.074786455510452	48.15562875959367	18.56947380121686	15.200110983679021
25	19.47375813313044	45.17940494480544	19.194058581804363	16.15277834025976
26	19.06666000305033	46.255291758686326	17.559117335081957	17.11893090318139
27	19.94625735977755	49.31613201198417	15.988327756863022	14.749282871375257
28	20.829042912253097	48.22176227950826	15.724224514467947	15.224970293770696
29	22.69732562077187	48.00617131295933	14.183636628405447	15.112866437863357
30	24.263892993810593	48.677157266802865	14.04141724779433	13.01753249159221
31	19.38517799179858	52.04492598646654	14.475615041735207	14.094280979999674
32	19.588877849880788	54.65114675954369	15.653610287015168	10.106365103560352
33	21.792438116689688	53.89739696742267	13.291717325948207	11.018447589939433
34	18.52582998644586	56.22546920332159	13.916000520451414	11.332700289781144
35	19.706151752947573	53.27276910310883	15.908019690986281	11.11305945295731
36	17.634169186346597	52.684977824806055	17.19402583817235	12.486827150674992
37	17.576264658160788	53.860086460421996	17.58436439871059	10.979284482706623
38	16.902952183095497	53.104871821605805	18.221917380923042	11.77025861437566
39	19.770906593619646	52.26215413461597	16.01129138299623	11.955647888768159
40	21.128819480048357	51.06158301673975	15.298255712255857	12.511341790956038
41	22.593967761309266	51.49534997341733	15.383992327650015	10.526689937623381
42	19.738852301231073	51.607367661872026	15.653911873099469	12.999868163797434
43	20.94101751698145	47.58696665578084	17.491217382387887	13.980798444849816
44	20.53094660978466	47.47947275859068	16.012282308701792	15.97729832292287
45	21.018869810457456	46.41271969469148	20.14745836173269	12.420952133118373
46	23.162759116301075	45.95818638192345	17.33055816690808	13.548496334867401
47	25.615774158553283	46.2617112339093	14.9753862671484	13.147128340389012
48	26.17530251238442	46.323881051001656	14.627959098033571	12.872857338580356
49	24.461776548838422	47.314548254204325	14.10918794930941	14.114487247647844
50	22.92420452361893	49.09528483082313	14.328742618680588	13.65176802687735
51	22.38992323341643	49.21165397563701	14.397245743543257	14.001177047403301
52	22.785302589935124	48.04619609471871	15.474468152940336	13.69403316240583
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	2.5
4	5.0
5	15.5
6	26.0
7	367.0
8	708.0
9	3588.0
10	6468.0
11	17469.0
12	28470.0
13	31604.5
14	36342.5
15	37946.0
16	32878.0
17	27810.0
18	30735.5
19	33661.0
20	38003.0
21	42345.0
22	52833.5
23	63322.0
24	79507.5
25	95693.0
26	108837.5
27	121982.0
28	126783.5
29	131585.0
30	123003.5
31	114422.0
32	109219.0
33	104016.0
34	100517.5
35	97019.0
36	92360.5
37	87702.0
38	88026.5
39	91306.0
40	94261.0
41	100958.0
42	107655.0
43	113348.0
44	119041.0
45	126555.0
46	134069.0
47	134300.0
48	134531.0
49	137268.0
50	140005.0
51	127195.5
52	114386.0
53	109799.5
54	105213.0
55	89216.0
56	73219.0
57	61767.5
58	50316.0
59	44181.5
60	38047.0
61	37638.0
62	37229.0
63	31777.0
64	18599.0
65	10873.0
66	9677.5
67	8482.0
68	7751.5
69	7021.0
70	5318.0
71	3615.0
72	2001.5
73	388.0
74	229.5
75	71.0
76	50.5
77	30.0
78	18.0
79	6.0
80	6.0
81	6.0
82	4.5
83	3.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2321062.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.68659682457009
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.33673447028904	35.12747906542613
2	6.09266547840724	4.957796478138636
3	2.0280024673232977	2.4753755624164917
4	1.0033889712335289	1.6329793012319496
5	0.6127623635437394	1.2465607617387382
6	0.44537213657676983	1.0872405934677838
7	0.32664083837867935	0.930293286828702
8	0.24810426481688394	0.8075614554448742
9	0.21836677401518334	0.7996140804813996
>10	2.326219880659379	20.329168543413616
>50	0.20441449497719225	5.554985577700918
>100	0.12303282338228515	10.629200926970617
>500	0.02035927557524372	5.978070869375467
>1k	0.013826887690138782	8.165135394837101
>5k	1.0887313141841562E-4	0.27853810252775324
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGT	6288	0.2709104711550144	No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGC	3052	0.1314915327552646	No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTA	2950	0.12709699266973482	No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTT	2836	0.1221854478682603	No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGG	2735	0.11783399150905921	No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGG	2495	0.10749389719016554	No Hit
AGTAGATCTTGGGCGTCCACCACCACAGCTGGCTAGGTTTTGTATTTTTAAT	2492	0.10736464601117937	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.024902393818002275	0.0	0.0	0.0	0.0
12	0.035242488136895955	0.0	0.0	0.0	0.0
13	0.0812128241296441	0.0	0.0	0.0	0.0
14	0.4987372159813051	0.0	0.0	0.0	0.0
15	0.9665834001849154	0.0	0.0	0.0	0.0
16	1.2206481343453988	0.0	0.0	0.0	0.0
17	1.4186609405522128	0.0	0.0	0.0	0.0
18	1.6741905214078727	0.0	0.0	0.0	0.0
19	1.9214049430820892	0.0	0.0	0.0	0.0
20	2.180251970865061	0.0	0.0	0.0	0.0
21	2.407217041164777	0.0	0.0	0.0	0.0
22	2.622204835545108	0.0	0.0	0.0	0.0
23	2.8268094518802167	0.0	0.0	0.0	0.0
24	3.043822181397998	0.0	0.0	0.0	0.0
25	3.2915966915144876	0.0	0.0	0.0	0.0
26	3.57168399637752	0.0	0.0	0.0	0.0
27	3.8446194026699847	0.0	0.0	0.0	0.0
28	4.135176053030897	0.0	0.0	0.0	0.0
29	4.453952544137123	0.0	0.0	0.0	0.0
30	4.805429583526851	0.0	0.0	0.0	0.0
31	5.108135844712463	0.0	0.0	0.0	0.0
32	5.3822345116158035	0.0	0.0	0.0	0.0
33	5.608768744652233	0.0	0.0	0.0	0.0
34	5.826815483601903	0.0	0.0	0.0	0.0
35	6.021467759155076	0.0	0.0	0.0	0.0
36	6.217024792961153	0.0	0.0	0.0	0.0
37	6.411289314977368	0.0	0.0	0.0	0.0
38	6.599392864128576	0.0	0.0	0.0	0.0
39	6.796888665619445	0.0	0.0	0.0	0.0
40	6.988352745424293	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTGTCC	35	1.0201802E-7	46.000004	37
CTAGATT	155	0.0	46.000004	36
CTATGTG	155	0.0	46.000004	23
CTCACCA	35	1.0201802E-7	46.000004	15
CATATTA	55	1.8189894E-12	46.000004	19
CTACTTC	55	1.8189894E-12	46.000004	39
CTGTATA	35	1.0201802E-7	46.000004	36
TTTACAT	155	0.0	46.000004	38
CAACGAC	35	1.0201802E-7	46.000004	20
ACACCTA	55	1.8189894E-12	46.000004	35
TAGGTTT	310	0.0	46.000004	34
CACATCG	155	0.0	46.000004	38
AGCCATA	55	1.8189894E-12	46.000004	22
ACACATT	35	1.0201802E-7	46.000004	28
TTGTCAC	35	1.0201802E-7	46.000004	23
CCTACTT	55	1.8189894E-12	46.000004	38
TATATTA	70	0.0	46.000004	34
CCTGTGC	140	0.0	46.000004	14
CAACAAC	35	1.0201802E-7	46.000004	32
ACTTACG	155	0.0	46.000004	42
>>END_MODULE