FastQCFastQC Report
Fri 17 Jun 2016
SRR1528132_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528132_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2164062
Sequences flagged as poor quality0
Sequence length59
%GC38

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGTTGCTACG46300.21394950791613182No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTACAGTCCA26620.12300941470253625No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTTGAGACCA23850.11020941174513484No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGCCTTCAGT23360.10794515129418658No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCC22940.10600435662194521No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGGCTCTGGG22010.10170688270483932No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAC3500.053.00000438
TAGTTCC1500.053.00000443
CGTTTAG750.053.00000419
ATCCTTA353.3438482E-853.036
AACGTTT800.053.022
CTAGATT1650.053.036
CTAGATA353.3438482E-853.023
ACTATCC550.053.049
TCCATAG650.053.023
TACCCGA150.006697062453.027
TACTTTG550.053.050
CTACTAA1200.053.035
TACTTAG307.0133865E-753.024
TGAAGTA353.3438482E-853.015
ACGTTTA800.053.023
ACCATTC401.6025297E-953.053
TTTACAC150.006697062453.025
ACTTGCT800.053.014
CTATCCA950.053.020
CCAGGTC457.6397555E-1153.016