##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528132_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2164062
Sequences flagged as poor quality	0
Sequence length	59
%GC	38
>>END_MODULE
>>Per base sequence quality	warn
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.117579810559956	31.0	30.0	31.0	27.0	34.0
2	31.136481764385678	31.0	31.0	34.0	28.0	34.0
3	31.44365364763117	31.0	31.0	34.0	30.0	34.0
4	35.22713073839844	35.0	35.0	37.0	33.0	37.0
5	35.04884656724253	37.0	35.0	37.0	32.0	37.0
6	34.4723871127537	35.0	35.0	37.0	30.0	37.0
7	34.76710694980088	35.0	35.0	37.0	32.0	37.0
8	34.8754374874657	35.0	35.0	37.0	32.0	37.0
9	37.081119210078086	39.0	37.0	39.0	33.0	39.0
10	37.4635398616121	39.0	37.0	39.0	35.0	39.0
11	37.134212420901065	39.0	37.0	39.0	34.0	39.0
12	37.19264882429432	39.0	37.0	39.0	34.0	39.0
13	37.12807304042121	39.0	37.0	39.0	34.0	39.0
14	36.91972919445006	40.0	36.0	40.0	31.0	40.0
15	36.150866749658746	38.0	35.0	40.0	30.0	40.0
16	35.60127205227946	38.0	34.0	40.0	30.0	40.0
17	35.47456449953837	38.0	34.0	40.0	27.0	40.0
18	35.68553581182055	38.0	34.0	40.0	27.0	40.0
19	36.051706928914236	38.0	34.0	40.0	30.0	40.0
20	36.092829595455214	38.0	34.0	40.0	30.0	40.0
21	36.08479193294831	38.0	35.0	40.0	30.0	40.0
22	36.02151786778752	38.0	35.0	40.0	30.0	40.0
23	35.90407391285462	38.0	34.0	40.0	29.0	40.0
24	35.99435690844347	38.0	35.0	40.0	30.0	40.0
25	36.01874992490973	38.0	35.0	40.0	30.0	40.0
26	35.63716011833302	38.0	34.0	40.0	27.0	40.0
27	35.35570746124649	38.0	34.0	40.0	26.0	40.0
28	34.958995167421264	37.0	34.0	40.0	25.0	40.0
29	34.67190496390584	37.0	33.0	40.0	25.0	40.0
30	34.36696222196961	36.0	33.0	40.0	25.0	40.0
31	34.269957145405264	36.0	33.0	40.0	25.0	40.0
32	34.19324122876332	35.0	33.0	40.0	25.0	40.0
33	33.84430205788929	35.0	33.0	40.0	23.0	40.0
34	33.53407157465914	35.0	33.0	39.0	21.0	40.0
35	33.38600049351636	35.0	33.0	39.0	21.0	40.0
36	33.00164921337743	35.0	32.0	39.0	20.0	40.0
37	32.93633084449521	35.0	32.0	39.0	20.0	40.0
38	32.71237099491604	35.0	31.0	39.0	18.0	40.0
39	32.15654634663887	35.0	31.0	39.0	15.0	40.0
40	31.65408107531115	35.0	30.0	39.0	15.0	40.0
41	30.97916325872364	35.0	28.0	38.0	11.0	40.0
42	30.440680535030882	35.0	26.0	38.0	10.0	40.0
43	29.859831649925002	35.0	24.0	38.0	9.0	40.0
44	29.26682692085532	34.0	23.0	38.0	8.0	40.0
45	28.89075821302717	34.0	22.0	38.0	8.0	40.0
46	28.41307966222779	33.0	20.0	38.0	8.0	40.0
47	27.874294729078926	33.0	18.0	37.0	8.0	40.0
48	27.422810437039235	33.0	17.0	37.0	8.0	40.0
49	26.99434997703393	33.0	15.0	36.0	8.0	40.0
50	26.425445758947756	33.0	13.0	35.0	8.0	39.0
51	26.015154371732418	32.0	11.0	35.0	8.0	39.0
52	25.28343226765222	31.0	10.0	35.0	8.0	39.0
53	25.04155888324826	31.0	10.0	35.0	8.0	39.0
54	24.747728114998555	30.0	10.0	35.0	8.0	39.0
55	24.457515080436696	30.0	10.0	35.0	8.0	38.0
56	23.961058879089418	29.0	9.0	35.0	8.0	38.0
57	23.4242054987334	27.0	9.0	35.0	8.0	38.0
58	22.95938979567129	26.0	9.0	35.0	8.0	37.0
59	22.186495118901398	25.0	8.0	34.0	8.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	4.0
13	13.0
14	54.0
15	221.0
16	847.0
17	2304.0
18	5421.0
19	10107.0
20	16751.0
21	24927.0
22	35717.0
23	48336.0
24	63078.0
25	79993.0
26	98810.0
27	116641.0
28	120061.0
29	105298.0
30	97631.0
31	105869.0
32	122629.0
33	140540.0
34	163380.0
35	184475.0
36	216771.0
37	217666.0
38	174401.0
39	12117.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.91228439850614	24.365383246875552	37.98962321781909	6.7327091367992224
2	25.100066449112823	22.09765709115543	34.10424470278578	18.698031756945966
3	18.456680076633663	23.362500704693304	36.332415614709745	21.848403603963288
4	20.929853211229624	21.099626535653783	34.442266441534485	23.528253811582108
5	100.0	0.0	0.0	0.0
6	0.0	100.0	0.0	0.0
7	0.0	0.0	100.0	0.0
8	0.0	0.0	0.0	100.0
9	0.0	0.0	100.0	0.0
10	0.0	0.0	100.0	0.0
11	99.41665257280059	0.40502536433799036	0.11792638103714219	0.06039568182427305
12	98.77850080080884	0.9004825185230368	0.14874804880821343	0.1722686318599005
13	94.28357413050088	3.6847835228380705	0.9156854101222608	1.115956936538787
14	45.17754112405282	35.05421748545097	10.960499283292254	8.807742107203953
15	29.34412230333512	41.396179961572265	18.922378379177676	10.337319355914941
16	35.20518358531318	32.86786607777411	18.504321964897493	13.422628372015218
17	20.225991676763417	36.264302963593465	21.55867068503583	21.95103467460729
18	17.44307695435713	46.415952962530646	20.497841559068085	15.643128524044137
19	22.81260888089158	44.73813596837798	21.49069666211042	10.958558488620012
20	19.020850604095447	43.26641288465857	23.0901425190221	14.622593992223884
21	24.021492914713164	44.05358996184028	14.989450394674458	16.9354667287721
22	25.32459790893237	45.43959461420236	17.18513610053686	12.050671376328404
23	16.428226178362728	47.31957772004684	20.870520345535386	15.38167575605505
24	17.71876221660932	48.177455174574476	18.83758413575951	15.266198473056686
25	19.3443625921993	44.97255623914656	19.492094034274434	16.1909871343797
26	18.751080144653894	46.33906052599232	17.87028282923502	17.03957650011876
27	19.809737428964606	49.56479989944835	16.14879795495693	14.476664716630113
28	20.719554245673184	48.52499604909656	15.713736482596156	15.041713222634101
29	22.614370567941215	48.226252297762265	14.07741552691189	15.081961607384631
30	24.213308121486353	49.03117378337589	13.914111518061867	12.841406577075887
31	19.14958998402079	52.509955814574624	14.341640858718465	13.998813342686114
32	19.377864404993943	55.38182362612532	15.476405019819211	9.763906949061534
33	21.621654093089752	54.460546878971115	13.24490703131426	10.672891996624866
34	18.17836087875486	56.9952709303153	13.920257367857298	10.906110823072538
35	19.641720061624852	53.954138097706995	15.731388472234158	10.672753368433991
36	17.31544659995878	53.27171772342937	17.295114465297203	12.117721211314649
37	17.134675439058586	54.732812645848405	17.51590296396314	10.616608951129866
38	16.604838493536693	53.981725107691005	17.96954061390108	11.443895784871229
39	19.37097920484718	53.19293070161576	15.779492454467569	11.65659763906949
40	20.714101536832125	52.288936268923905	14.877577444638831	12.11938474960514
41	22.159023170315823	52.73702879122687	15.032794809021183	10.071153229436124
42	19.091550981441383	53.20508377301575	15.189721921090985	12.513643324451888
43	20.03815047812863	49.081172350884586	17.248442974369496	13.632234196617288
44	19.65410418000963	48.995084244351595	15.523353767128667	15.827457808510106
45	20.143184437414455	47.90643706141506	19.742040662420948	12.208337838749536
46	22.334295412978	47.466800858755434	16.961944713229105	13.236959015037462
47	24.889767483556387	47.54457127383596	14.629340564179769	12.936320678427881
48	25.2715957306214	47.724325828003074	14.216921696328477	12.787156745047046
49	23.981198320565678	48.511780161566534	13.608390147786894	13.898631370080894
50	21.869890973548817	50.39107012645664	14.149779442548319	13.589259457446229
51	21.631773951023582	50.395413809770695	14.125103624572679	13.847708614633037
52	20.249789516196856	50.48339650157897	15.488927766394864	13.777886215829307
53	19.806271724192744	50.21154661927431	14.63299110653946	15.349190549993486
54	18.959900409507675	50.791566969892735	15.1622273299009	15.086305290698695
55	20.552784532051298	48.798879144867385	14.79541713684728	15.852919186234034
56	20.0971136686472	48.020112177932056	15.952870111854466	15.929904041566276
57	21.067002701401346	45.89170735404069	15.998293949064307	17.04299599549366
58	21.321200594068006	43.91084913463663	17.271917348024225	17.496032923271144
59	21.300313946642934	42.338528193739364	18.145968091487212	18.21518976813049
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	6.0
4	14.0
5	16.0
6	254.5
7	493.0
8	2690.5
9	12880.5
10	20873.0
11	23059.5
12	25246.0
13	25905.5
14	21589.0
15	16613.0
16	16599.5
17	16586.0
18	16321.0
19	18278.5
20	20501.0
21	26370.5
22	32240.0
23	40738.5
24	49237.0
25	61452.5
26	83162.0
27	92656.0
28	101357.0
29	110058.0
30	114876.0
31	118338.5
32	116983.0
33	117164.5
34	117346.0
35	111803.5
36	103676.0
37	101091.0
38	101089.0
39	101087.0
40	96681.0
41	92275.0
42	90568.5
43	96177.0
44	103492.0
45	102837.5
46	102183.0
47	100311.0
48	98722.5
49	99006.0
50	92361.5
51	85717.0
52	79565.5
53	65295.0
54	57176.0
55	52694.5
56	48213.0
57	41909.0
58	34779.0
59	33953.0
60	30830.5
61	27708.0
62	24606.0
63	21504.0
64	14145.5
65	7242.0
66	7697.0
67	6110.5
68	4524.0
69	5734.5
70	3937.0
71	929.0
72	1067.5
73	1206.0
74	700.5
75	116.5
76	38.0
77	23.5
78	9.0
79	9.5
80	10.0
81	6.0
82	2.5
83	3.0
84	1.5
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
53	0.0
54	0.0
55	0.0
56	0.0
57	0.0
58	0.0
59	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
59	2164062.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.586198123783646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	91.65494267029251	50.03094859624618
2	3.911574464385433	4.270359573777523
3	1.23785651066717	2.0270964222028094
4	0.6364830889299822	1.3897276797907923
5	0.3779363384481823	1.0315053924354916
6	0.2596841347414386	0.8505101777159695
7	0.18781832999122508	0.7176602000525432
8	0.14547410007438374	0.6352702438831551
9	0.1195320489679706	0.5872320096396706
>10	1.272179839706279	14.648451584671454
>50	0.1024235101917024	3.7713301883350114
>100	0.07159901612143409	8.555773468350006
>500	0.01606853391582274	6.341481413871741
>1k	0.006427413566329096	5.142653049027709
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CACTGATCTTGGGTTCTCGAGCGGCGGCCCCAGGGTGCGGTTTCTCACGAGTTGCTACG	4630	0.21394950791613182	No Hit
CTAGGATCTTGGGGCCTGGGAAATCCCATGGACAGAGGAGCCTGGTGGGCTACAGTCCA	2662	0.12300941470253625	No Hit
ATGAGATCTTGGGGGAGGCCAAGGTGGGCGGATCACCTGAGGTCAGGAGTTTGAGACCA	2385	0.11020941174513484	No Hit
TGCGGATCTTGGGGTCCACCATGGTGTTAACCAGCTGAAATTTGCCCGAAGCCTTCAGT	2336	0.10794515129418658	No Hit
GCATGATCTTGGGCTTTCGCCGCCGGACGCCGCCGAGGTCGCACGCGTGAGGTCTGTCC	2294	0.10600435662194521	No Hit
TGCGGATCTTGGGGGGAGAAGGAAAGGAAAATGGGGAAAAGGGAGAGGTGGGCTCTGGG	2201	0.10170688270483932	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0237516300364777	0.0	0.0	0.0	0.0
12	0.03230036847373134	0.0	0.0	0.0	0.0
13	0.0747668042782508	0.0	0.0	0.0	0.0
14	0.4243871016634459	0.0	0.0	0.0	0.0
15	0.8174904415862392	0.0	0.0	0.0	0.0
16	1.041513598039243	0.0	0.0	0.0	0.0
17	1.2108710378907812	0.0	0.0	0.0	0.0
18	1.4308739768084278	0.0	0.0	0.0	0.0
19	1.6468104887937591	0.0	0.0	0.0	0.0
20	1.8642257014817505	0.0	0.0	0.0	0.0
21	2.0578892841332643	0.0	0.0	0.0	0.0
22	2.238475607445628	0.0	0.0	0.0	0.0
23	2.415087922619592	0.0	0.0	0.0	0.0
24	2.6046388689418327	0.0	0.0	0.0	0.0
25	2.804817976564442	0.0	0.0	0.0	0.0
26	3.033970376079798	0.0	0.0	0.0	0.0
27	3.2567920882118906	0.0	0.0	0.0	0.0
28	3.496942324203281	0.0	0.0	0.0	0.0
29	3.766019642690459	0.0	0.0	0.0	0.0
30	4.059726569756319	0.0	0.0	0.0	0.0
31	4.326585837189508	0.0	0.0	0.0	0.0
32	4.555599608513989	0.0	0.0	0.0	0.0
33	4.754300015433938	0.0	0.0	0.0	0.0
34	4.943758542962263	0.0	0.0	0.0	0.0
35	5.120278439342311	0.0	0.0	0.0	0.0
36	5.283397610604502	0.0	0.0	0.0	0.0
37	5.450583208799008	0.0	0.0	0.0	0.0
38	5.609728371922801	0.0	0.0	0.0	0.0
39	5.779825162125669	0.0	0.0	0.0	0.0
40	5.950060580519412	0.0	0.0	0.0	0.0
41	6.1068490643983395	0.0	0.0	0.0	0.0
42	6.267380509430875	0.0	0.0	0.0	0.0
43	6.425462856424631	0.0	0.0	0.0	0.0
44	6.588535818289865	0.0	0.0	0.0	0.0
45	6.749252100910232	0.0	0.0	0.0	0.0
46	6.911862968805885	0.0	0.0	0.0	0.0
47	7.06426155997379	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGCAC	350	0.0	53.000004	38
TAGTTCC	150	0.0	53.000004	43
CGTTTAG	75	0.0	53.000004	19
ATCCTTA	35	3.3438482E-8	53.0	36
AACGTTT	80	0.0	53.0	22
CTAGATT	165	0.0	53.0	36
CTAGATA	35	3.3438482E-8	53.0	23
ACTATCC	55	0.0	53.0	49
TCCATAG	65	0.0	53.0	23
TACCCGA	15	0.0066970624	53.0	27
TACTTTG	55	0.0	53.0	50
CTACTAA	120	0.0	53.0	35
TACTTAG	30	7.0133865E-7	53.0	24
TGAAGTA	35	3.3438482E-8	53.0	15
ACGTTTA	80	0.0	53.0	23
ACCATTC	40	1.6025297E-9	53.0	53
TTTACAC	15	0.0066970624	53.0	25
ACTTGCT	80	0.0	53.0	14
CTATCCA	95	0.0	53.0	20
CCAGGTC	45	7.6397555E-11	53.0	16
>>END_MODULE