FastQCFastQC Report
Fri 17 Jun 2016
SRR1528131_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528131_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1299204
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAGCGAATGGGCATGTGATACTACTAAGAACAACAGTTTTGCGGTGGCTC28830.22190510497196747No Hit
ATTGGCGAATGGGCGCAGAGTCGTTGCGAATGGGGGCCTATGGCGGCGTTTA27630.21266868020726534No Hit
CCGTGCGAATGGGATTGAGATGTGGCCAGGCGCGGTGGCTCACGCCTGTAAT27240.2096668421587372No Hit
GTATGCGAATGGGGCTGACCCTGCGCGCCGCGGGAAGATGGCGCTGGAAGCT26830.206511063697464No Hit
ATATGCGAATGGCCTGTCGTCACTACACAACACCCTTCATACAAATAACATA23420.1802642233244356No Hit
ATGAGCGAATGGGTATACCACCATGCCCAACCACTTTTATTATTAATGATTG22990.17695450445041733No Hit
TCGAGCGAATGGGGGGGGTTCCCCCAAAAAACTAAAAATAGAACTACCATAT22870.17603086197394713No Hit
TGTGGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTT21680.16687140741561757No Hit
GTTCGCGAATGGGGCGTGATCTAACTGCTCATCTGGACCGCGCCAGGAGGCT20840.1604059100803261No Hit
GGGCGCGAATGGGGCGGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG20540.15809680388915057No Hit
AAGGGCGAATGGGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAGTG20460.15748104223817044No Hit
ACTGGCGAATGGGGGCACGGTAGCTCACACCTATAATCCCAGCACTTTGGGA20310.15632648914258268No Hit
CGTGGCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGC19880.15301677026856444No Hit
TCGGGCGAATGGGCAGTGGCTCACCCCTGTAATCCCAGCAATTTGGAAGGCC19870.15293980006219193No Hit
CGCTGCGAATGGGGGTTTTGAGCAGTTGACTTAGGCCGTTCTCTCAGCCCAA19720.15178524696660417No Hit
TGTTGCGAATGGGGTGTGTGCGAATGGGCATACTTGAGGAGATCTGACTCCC19210.14785976644160578No Hit
ATGCGCGAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG19080.14685915375876307No Hit
ACGCGCGAATGGGGGGGCAAGGCAGTCTCAATATCATCTACATAAAAGGGGC19010.14632036231415543No Hit
TGGCGCGAATGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGAT18960.14593551128229285No Hit
CTTTGCGAATGGGCAACCCCAAATGCAAGCAAACAGACAAACAAATATACAA18760.1443961071548425No Hit
AGAAGCGAATGGGGCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATG18570.14293367323376466No Hit
TTGCGCGAATGGGGTTGTTCCGCCTATAGGGTTGCAGATCCCTTTAGCTCCT18470.14216397117003948No Hit
TTTAGCGAATGGGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAC18370.1413942691063143No Hit
TTACGCGAATGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGC18330.14108638828082426No Hit
GCGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATG18130.1395469841533739No Hit
ATTTGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTT18110.13939304374062889No Hit
ACGCGCGAATGGGGCGCCCCATCACCCAGAGTGAGCTGGCCACTGCCCTGGC17850.13739181837494344No Hit
CCGAGCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG17490.1346208909455328No Hit
CGTAGCGAATGGGGGTGGTCCGACCGGAAATTCGGTTGTTAGATCAAGCGGT17380.13377421867543512No Hit
AAACGCGAATGGGATGCTCAAGGTTTTGGAATTCATCTTAACCTGTGCTTCT17370.1336972484690626No Hit
CTAAGCGAATGGGGGCTGGTCCCCTGCCCAACTTGGGACCAAGGAAAATCAG17180.13223481454798477No Hit
TGAGGCGAATGGGATCAGGAGATCGAGACCACCCTGGCTAACATGGTGAAAC17040.13115723165876952No Hit
AGAGGCGAATGGGACCCGGATACAGCAGCAGCTGGGCCAGCTGACCCTGGAA16860.1297717679440642No Hit
GGGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCC16700.12854024464210395No Hit
TTGGGCGAATGGGGGTAATGAGGTCCAGCTAGAATGTTTTCAAGACTCCTTT16440.12653901927641847No Hit
CAGAGCGAATGGGGACAGCCCTGCAGCCCTGTGCGGCACGTCCAGCATGTTG16120.12407597267249794No Hit
GTACGCGAATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCG15680.12068928359210718No Hit
CTTGGCGAATGGGGACTAAAGCCATTGAGCTGGCTCGGCAAGTGAAGGCATC15440.11884199863916675No Hit
TGATGCGAATGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA15430.11876502843279424No Hit
TGCGGCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG15380.11838017740093164No Hit
TTTGGCGAATGGGGCTGCATTGCTGAATGTTCAGCAGGCCAGGCACTGTCAT14910.11476257770142333No Hit
AGTCGCGAATGGGAGTGGGCGCCCAGTTACCAGCAAGTGTACACAGGGACCA14830.11414681605044319No Hit
ATGTGCGAATGGGAGACACTCCTCAGTACCATCCTTGAGCCCAGGAGGCTCT14800.11391590543132564No Hit
GCTTGCGAATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC14770.11368499481220809No Hit
AATTGCGAATGGGGACTCTGAATACCACACTACAAGAGGAACAAACCACATT14720.1133001437803455No Hit
CCATGCGAATGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGAT14680.11299226295485544No Hit
CGGGGCGAATGGGGCAGATCTGGTCGCCCATGAGAACTATAGGGACGTGGTT14490.1115298290337776No Hit
TTTTGCGAATGGGTATTAACTTCTTGCGAAAAAGGATTCTCCCGGCTGAGCT14210.10937466325534713No Hit
TGCGGCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG14180.10914375263622958No Hit
AAGTGCGAATGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTA13990.10768131871515174No Hit
GTTCGCGAATGGGTTCCGTATTACAGTTTTAGTATTTTTATCTTAAGAAGGA13950.10737343788966168No Hit
TTGGGCGAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC13950.10737343788966168No Hit
GAATGCGAATGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC13920.10714252727054412No Hit
GCCGGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATG13910.10706555706417159No Hit
TCTAGCGAATGGGGCAGAGTCTCGGCGAATGGGATCAACGCAGAGTTTGGGC13870.10675767623868153No Hit
ATTCGCGAATGGGACCATCTGCCTTGCTTACCTCATGAGGACTAACCGGGTA13370.10290916592005565No Hit
GAATGCGAATGGGGAACGGAGGCAGGTTGGAGCCGCTGCCGTCGCCATGACC13290.10229340426907553No Hit
ATGGGCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAG13220.1017546128244679No Hit
TTGAGCGAATGGGATCATCGACACCTTCCATCAATACTCTAGGAAGGAAGGA13100.10083097034799769No Hit
AAGCGCGAATGGGACCTGGACCCCCGTGGGCGCCTACGGAGTGCAGACAGTG13090.10075400014162518No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACAC2850.046.00000427
GCGTTTA2950.046.00000446
GCCCTGG2850.046.00000445
ACTACAC2850.046.00000422
CCCTTCA2850.046.00000433
CACAACA2850.046.00000426
TCGGTCT4650.046.00000418
AACACCC2850.046.00000429
TCCGGCA501.6370905E-1146.020
CTTAATG206.31176E-446.030
TTGATTG551.8189894E-1246.026
CCTTCCT600.046.016
CCTTCAG1200.046.028
TTTAGTA1700.046.028
TCGCCGA206.31176E-446.020
TTGATGT1000.046.042
AACGTTT206.31176E-446.037
CTTAACT501.6370905E-1146.023
CTGCTCT1100.046.020
TATTTAT206.31176E-446.036
Produced by FastQC (version 0.11.5)