FastQCFastQC Report
Fri 17 Jun 2016
SRR1528130_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528130_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296297
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAGCGAATGGGCATGTGATACTACTAAGAACAACAGTTTTGCGGTGGCTC28940.2232513073778617No Hit
ATTGGCGAATGGGCGCAGAGTCGTTGCGAATGGGGGCCTATGGCGGCGTTTA28820.22232559359467774No Hit
CCGTGCGAATGGGATTGAGATGTGGCCAGGCGCGGTGGCTCACGCCTGTAAT26820.206897030541612No Hit
GTATGCGAATGGGGCTGACCCTGCGCGCCGCGGGAAGATGGCGCTGGAAGCT26720.2061256023889587No Hit
TCGAGCGAATGGGGGGGGTTCCCCCAAAAAACTAAAAATAGAACTACCATAT22840.1761941900660111No Hit
ATGAGCGAATGGGTATACCACCATGCCCAACCACTTTTATTATTAATGATTG22480.17341704871645927No Hit
ATATGCGAATGGCCTGTCGTCACTACACAACACCCTTCATACAAATAACATA22230.17148847833482606No Hit
GTTCGCGAATGGGGCGTGATCTAACTGCTCATCTGGACCGCGCCAGGAGGCT21270.16408276806935448No Hit
TGTGGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTT21040.16230848331825193No Hit
GGGCGCGAATGGGGCGGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG20800.16045705575188401No Hit
AAGGGCGAATGGGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAGTG20270.1563684865428216No Hit
ACTGGCGAATGGGGGCACGGTAGCTCACACCTATAATCCCAGCACTTTGGGA19720.15212563170322851No Hit
TCGGGCGAATGGGCAGTGGCTCACCCCTGTAATCCCAGCAATTTGGAAGGCC19500.15042848976739126No Hit
TGTTGCGAATGGGGTGTGTGCGAATGGGCATACTTGAGGAGATCTGACTCCC19120.14749706278730876No Hit
CGTGGCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGC19070.14711134871098214No Hit
CGCTGCGAATGGGGGTTTTGAGCAGTTGACTTAGGCCGTTCTCTCAGCCCAA18880.14564563522094087No Hit
ATGCGCGAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAG18880.14564563522094087No Hit
ACGCGCGAATGGGGGGGCAAGGCAGTCTCAATATCATCTACATAAAAGGGGC18870.14556849240567554No Hit
AGAAGCGAATGGGGCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATG18810.1451056355140836No Hit
TTGCGCGAATGGGGTTGTTCCGCCTATAGGGTTGCAGATCCCTTTAGCTCCT18360.14163420882714378No Hit
CCGAGCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGG18060.13931992436918392No Hit
TTACGCGAATGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGC17970.13862563903179595No Hit
TGGCGCGAATGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGAT17900.13808563932493867No Hit
ACGCGCGAATGGGGCGCCCCATCACCCAGAGTGAGCTGGCCACTGCCCTGGC17840.1376227824333467No Hit
CTTTGCGAATGGGCAACCCCAAATGCAAGCAAACAGACAAACAAATATACAA17720.13669706865016273No Hit
AAACGCGAATGGGATGCTCAAGGTTTTGGAATTCATCTTAACCTGTGCTTCT17630.13600278331277477No Hit
GCGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATG17280.13330278477848825No Hit
CGTAGCGAATGGGGGTGGTCCGACCGGAAATTCGGTTGTTAGATCAAGCGGT17130.13214564254950834No Hit
TTTAGCGAATGGGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTAC17070.13168278565791636No Hit
TGAGGCGAATGGGATCAGGAGATCGAGACCACCCTGGCTAACATGGTGAAAC16940.1306799290594671No Hit
ATTTGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTT16610.12813421615571122No Hit
CTAAGCGAATGGGGGCTGGTCCCCTGCCCAACTTGGGACCAAGGAAAATCAG16330.12597421732828204No Hit
AGAGGCGAATGGGACCCGGATACAGCAGCAGCTGGGCCAGCTGACCCTGGAA16320.12589707451301668No Hit
CAGAGCGAATGGGGACAGCCCTGCAGCCCTGTGCGGCACGTCCAGCATGTTG15760.12157707685815829No Hit
GGGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCC15750.12149993404289294No Hit
CTTGGCGAATGGGGACTAAAGCCATTGAGCTGGCTCGGCAAGTGAAGGCATC15520.11972564929179039No Hit
TTGGGCGAATGGGGGTAATGAGGTCCAGCTAGAATGTTTTCAAGACTCCTTT15400.11879993550860644No Hit
TGCGGCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGAGACTCCTGCG15360.11849136424754513No Hit
GCTTGCGAATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTC15350.1184142214322798No Hit
GTACGCGAATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCG15250.1176427932796265No Hit
AATTGCGAATGGGGACTCTGAATACCACACTACAAGAGGAACAAACCACATT14800.1141713665926867No Hit
CCATGCGAATGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGAT14740.11370850970109474No Hit
AGTCGCGAATGGGAGTGGGCGCCCAGTTACCAGCAAGTGTACACAGGGACCA14580.11247422465684948No Hit
TTTGGCGAATGGGGCTGCATTGCTGAATGTTCAGCAGGCCAGGCACTGTCAT14580.11247422465684948No Hit
TGATGCGAATGGGCTCTTCGGACTTGGGCTTGGGTTCCGGCGACATGGCTAA14540.11216565339578816No Hit
TTTTGCGAATGGGTATTAACTTCTTGCGAAAAAGGATTCTCCCGGCTGAGCT14380.1109313683515429No Hit
ATGTGCGAATGGGAGACACTCCTCAGTACCATCCTTGAGCCCAGGAGGCTCT14240.10985136893782829No Hit
CGGGGCGAATGGGGCAGATCTGGTCGCCCATGAGAACTATAGGGACGTGGTT14040.10830851263252171No Hit
TCTAGCGAATGGGGCAGAGTCTCGGCGAATGGGATCAACGCAGAGTTTGGGC14040.10830851263252171No Hit
GAATGCGAATGGGGCCTTTCCGGCGGTGACGACCTCCCTACGAGAACATGCC14010.10807708418672574No Hit
TGCGGCGAATGGGGAGTGACGAGAGGCTTTGTCCGGTTCGGCGGTCTTTCTG13890.10715137040354177No Hit
GCCGGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATG13680.10553137128296987No Hit
GTTCGCGAATGGGTTCCGTATTACAGTTTTAGTATTTTTATCTTAAGAAGGA13610.10499137157611257No Hit
GAATGCGAATGGGGAACGGAGGCAGGTTGGAGCCGCTGCCGTCGCCATGACC13600.10491422876084724No Hit
ATGGGCGAATGGGGGTCTCTTGCTGCAGCAACGAGAGTGGGAGCACCTGGAG13510.10421994342345929No Hit
TTGGGCGAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGC13330.10283137274868337No Hit
TTGAGCGAATGGGATCATCGACACCTTCCATCAATACTCTAGGAAGGAAGGA13170.10159708770443811No Hit
AAGTGCGAATGGGCTTTGTCTCGGGCCTGAGTTCGTAGGCGCAGCCGGCCTA13020.10043994547545816No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCTGAT551.8189894E-1246.00000444
TGATCCG351.0197073E-746.00000441
TTGATCC351.0197073E-746.00000421
TGTTTCT551.8189894E-1246.00000427
ATCCTAA1100.046.00000419
GAAATGT351.0197073E-746.00000416
ACTATTG1100.046.00000444
CTGTCAT1400.046.00000446
CTGTCAG551.8189894E-1246.00000429
CTCACAG1450.046.00000425
ACCGGCC551.8189894E-1246.00000441
GTATTTC551.8189894E-1246.00000440
TTAATTG700.046.00000437
ATGATCT351.0197073E-746.00000445
AATCAAT700.046.00000425
GCGGTTA351.0197073E-746.00000427
ACCATTT1100.046.00000416
CCGATGT551.8189894E-1246.00000415
ACTTGAT700.046.00000443
TAGACAT551.8189894E-1246.00000441
Produced by FastQC (version 0.11.5)