FastQCFastQC Report
Fri 17 Jun 2016
SRR1528129_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528129_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1184098
Sequences flagged as poor quality0
Sequence length59
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCAGCGAATGGGCATGTGATACTACTAAGAACAACAGTTTTGCGGTGGCTCACGCCTA25100.21197569795743257No Hit
ATATGCGAATGGCCTGTCGTCACTACACAACACCCTTCATACAAATAACATAACCAGCC24340.20555731029019558No Hit
CCGTGCGAATGGGATTGAGATGTGGCCAGGCGCGGTGGCTCACGCCTGTAATCCCAGAA22140.18697776704293056No Hit
GTATGCGAATGGGGCTGACCCTGCGCGCCGCGGGAAGATGGCGCTGGAAGCTGGCGACA21060.17785690035790955No Hit
ATTGGCGAATGGGCGCAGAGTCGTTGCGAATGGGGGCCTATGGCGGCGTTTATTTGCTT19820.16738479416399657No Hit
TCGAGCGAATGGGGGGGGTTCCCCCAAAAAACTAAAAATAGAACTACCATATGATTCAG19450.1642600527996838No Hit
ATGAGCGAATGGGTATACCACCATGCCCAACCACTTTTATTATTAATGATTGATTTTTT18760.15843283241758704No Hit
AAGGGCGAATGGGGAGTCCGGCTCTCGCAACCCAAGATGGCGGATGAGAGTGAGACAGC18650.1575038552552238No Hit
GTTCGCGAATGGGGCGTGATCTAACTGCTCATCTGGACCGCGCCAGGAGGCTCCCGACA18450.1558148058691088No Hit
TGTGGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAAGCACA16590.1401066465782393No Hit
ATGCGCGAATGGGGCCGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG16230.1370663576832323No Hit
AAACGCGAATGGGATGCTCAAGGTTTTGGAATTCATCTTAACCTGTGCTTCTGGTTGCC16200.13681300027531507No Hit
TGTTGCGAATGGGGTGTGTGCGAATGGGCATACTTGAGGAGATCTGACTCCCTCTTCTG16200.13681300027531507No Hit
CTTTGCGAATGGGCAACCCCAAATGCAAGCAAACAGACAAACAAATATACAAGGCTGGT16160.13647519039809206No Hit
TCGGGCGAATGGGCAGTGGCTCACCCCTGTAATCCCAGCAATTTGGAAGGCCAAGGTGG15830.1336882589110023No Hit
TTACGCGAATGGGGATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACAAA15410.1301412552001608No Hit
CGCTGCGAATGGGGGTTTTGAGCAGTTGACTTAGGCCGTTCTCTCAGCCCAAAGTCCCC15160.12802994346751706No Hit
ACTGGCGAATGGGGGCACGGTAGCTCACACCTATAATCCCAGCACTTTGGGAGGCTGAG15110.1276076811209883No Hit
CTAAGCGAATGGGGGCTGGTCCCCTGCCCAACTTGGGACCAAGGAAAATCAGAGAATTG14890.1257497267962618No Hit
ATTTGCGAATGGGGATGAACTTCCTTGAAACAAGAGCTTCCTTGCTTCCTTTAAGCACA14820.12515855951112154No Hit
AGAAGCGAATGGGGCCTTTTGCGGCCATCGGTGGATCGCAGCCGCCAAAATGAAGTTCA14680.12397622494084105No Hit
CGTGGCGAATGGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCG14590.1232161527170893No Hit
ACGCGCGAATGGGGGGGCAAGGCAGTCTCAATATCATCTACATAAAAGGGGCCAAGAGT14520.12262498543194904No Hit
GCGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACA14440.12194936567750306No Hit
GGGTGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAGCAAGAAGATGGCCAACAAAG14420.12178046073889155No Hit
CCATGCGAATGGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAAC14390.1215271033309743No Hit
ACGCGCGAATGGGGCGCCCCATCACCCAGAGTGAGCTGGCCACTGCCCTGGCCCTGGCC14240.12026031629138806No Hit
TTTAGCGAATGGGACCATCCTGGCCAACATGGTGAAACCCCGTCTCTACTACAAATACA14220.12009141135277655No Hit
TTGCGCGAATGGGGTTGTTCCGCCTATAGGGTTGCAGATCCCTTTAGCTCCTTGGGTAC14070.11882462431319028No Hit
AGAGGCGAATGGGACCCGGATACAGCAGCAGCTGGGCCAGCTGACCCTGGAAAATCTCC14060.11874017184388454No Hit
TGAGGCGAATGGGATCAGGAGATCGAGACCACCCTGGCTAACATGGTGAAACCCCATCT13750.11612214529540629No Hit
AATTGCGAATGGGGACTCTGAATACCACACTACAAGAGGAACAAACCACATTCTAAGTC13440.11350411874692803No Hit
CAGAGCGAATGGGGACAGCCCTGCAGCCCTGTGCGGCACGTCCAGCATGTTGTTTGCCT13250.1118995218301188No Hit
TTGGGCGAATGGGGGTAATGAGGTCCAGCTAGAATGTTTTCAAGACTCCTTTGAGTCAG12910.1090281378737233No Hit
ATGTGCGAATGGGAGACACTCCTCAGTACCATCCTTGAGCCCAGGAGGCTCTGCTGGGA12880.10877478046580605No Hit
GCCAGCGAATGGGGAAATATCCTAAATGCCCATCAGTAGGGGAAAAGTTAAATGAATGA12770.10784580330344279No Hit
CCGAGCGAATGGGGCTCTCGGTCTTAGCGCCATCTTCCTTGGTTAGTCGCGGGGTTTTC12740.10759244589552554No Hit
TTGGGCGAATGGGAGGCATTCAGGCAGCGAGAGCAGAGCAGCGTAGAGCAGCACAGCTG12550.10598784897871628No Hit
GCTTGCGAATGGGGCCTTTCTCCTCGTTGGGCGTCTGAAGGCAAGATGGGTCACCAGCA12490.10548113416288178No Hit
CTTGGCGAATGGGGACTAAAGCCATTGAGCTGGCTCGGCAAGTGAAGGCATCTGGCGTC12460.10522777675496454No Hit
GGGCGCGAATGGGGCGGGTAGTGGTGGCGCACGCCGGTAGGATTTGCTGAAGGAGGCAG12300.10387653724607253No Hit
TGGCGCGAATGGGAGGCGATACGACGACGAGTCGTCGGAGACACGCAGGGATGAGATGG12170.10277865514509779No Hit
GTACGCGAATGGGCTCTTTCTTGACTCCATCTTCGCGGTAGCAGCAGCGGCGTCCGCGG12140.10252529773718054No Hit
CGTAGCGAATGGGGGTGGTCCGACCGGAAATTCGGTTGTTAGATCAAGCGGTGGTACAG12100.10218748785995753No Hit
GCCGGCGAATGGGAACATCTGTGACTCTTTAGCCTTGAAGAGCAAGAAGATGGCCAACA12000.10134296316690004No Hit
TTTTGCGAATGGGTATTAACTTCTTGCGAAAAAGGATTCTCCCGGCTGAGCTAAAACAG11900.10049843847384253No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT750.053.00000451
ACTATTG750.053.00000444
GACAGTA1750.053.00000414
CCTTATG750.053.00000420
CGGCGAG750.053.00000419
CGTGCTT750.053.00000419
GCAACAC1750.053.00000427
CAACCAC1750.053.00000428
CCCAACC1750.053.00000426
TGACCCG1550.053.00000448
CACTATT750.053.00000443
TAACATA2950.053.00000446
TTCCACG750.053.00000429
TTGGTTA1500.053.00000439
TTTATTA1750.053.00000436
CCAACCA1750.053.00000427
CAGTACG1750.053.00000416
CAGTACC1500.053.00000424
TGTACAC1550.053.00000438
TTACAGG1550.053.00000443
Produced by FastQC (version 0.11.5)