FastQCFastQC Report
Fri 17 Jun 2016
SRR1528128_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528128_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467862
Sequences flagged as poor quality0
Sequence length52
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAGTTTAGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG47821.02209625915334No Hit
GGTCAGTTTAGGGGGCGATCACCAGCCCACCGCACAGAAGGATGAGGACCGC24710.5281471887009418No Hit
TGTGAGTTTAGGGACCTAACATTTAATTAAAAAGCCCTCTTAAAAGTGATTG18740.4005454599860642No Hit
ATTTAGTTTAGGGCTCCAGCCTGGGTGATCAAGTGAGACCCTGTCTCAAAAA18320.39156845394582157No Hit
CGTGAGTTTAGGGGTGAAATACTGTACAGTATCCCCCTAAAATCAGGAACAT15620.33385912940140466No Hit
ACTTAGTTTAGGGGTGCATTCTAGATTCAGTAAAATTCCAGTAGTAACAAGA14240.304363252412036No Hit
CTTAAGTTTAGGGAGGTCTAACACAAAGAAAAATTGTTCTGATCTACAGTTT13820.2953862463717934No Hit
TTATAGTTTAGGGCAGAAACTGCAATTCTCGGTTGTCAGGGAAGCAATGTTT13800.2949587698936866No Hit
GTATAGTTTAGGGGGTCAGGAGATCAAGACCATCCTGGCTAACACATGAAAC12850.27465363718361396No Hit
TTAGAGTTTAGGGGACCAGGAGTTCAAAACCAGCCTGGCTAACATGGCGAGA12830.27422616070550715No Hit
GGCGAGTTTAGGGGATATTTTAAAGAACTGAATTTAATACCACCACATCCAT12210.2609743898841966No Hit
TCGCAGTTTAGGGTGTACCCTGATTTGACTTAGTGATCAAACTGTGCAGCAC11840.25306607503922096No Hit
CCATAGTTTAGGGGTTTGATATAATATGCATTTCGAGCCTTGGTTGCATTAC11470.24515776019424532No Hit
ATTTAGTTTAGGGGTTCTATTTTTTCTATAGTCCTTACATTGGATTATCATT10490.22421141276701248No Hit
GATAAGTTTAGGGGGAGGCCAAGGCAGAGGATCACTTGAGCCCTTGAGTTCA10260.2192954332687844No Hit
GGCGAGTTTAGGGTTGTGCACATGTACCCTAGAACTTAAAGTATAATAAAAA9960.2128832860971825No Hit
TGCAAGTTTAGAGACTACATACTGCACAGAACTTCTTTTCTATATATAAAGT9950.2126695478581291No Hit
GTGGAGTTTAGGGGTCATTTTGCGCCCCAGCACATCAAAATAACCGCTTAAC9940.2124558096190757No Hit
ACTTAGTTTAGGGCGGCACGAAGTGTCTTCCGATGCAGTGTCCAGTCATGAC9900.2116008566628621No Hit
GACAAGTTTAGGGCTCTAGGACTCAGTAGAGACATTAGTAGACATGGCATTA9870.21095964194570194No Hit
TTTGAGTTTAGGGGTAATTAATTTAAAATTCCATTAAAATGAAAAAATAATT9850.21053216546759512No Hit
TCGTAGTTTAGGGGGCCGAGGCGGGCAGATCACAAGGTCTGGAGTTCGAGAC9830.21010468898948836No Hit
GTTCAGTTTAGGGAGCCTGCCCAACACAGTGAAAATGTGTCTCTACTAAAAA9500.2030513271007263No Hit
TGTTAGTTTAGGGGAACACGAAAGTCTGCAATGGGGAGACAGACACTAATCA9210.1968529181681778No Hit
CGGCAGTTTAGGGATCAGACTAACGACCACCAGGCCCAGCGACAGGGCACTG8880.18979955627941572No Hit
TTTTAGTTTAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC8340.17825769137053232No Hit
GCTTAGTTTAGGGGAGGGATGGAGTCACAATCACACAGGGGAAAAGGTTGAA8340.17825769137053232No Hit
ATATAGTTTAGGGTTCCTGCTTGGTCTTGGCGTTACGCAACCGAAGCTGGAT8290.17718900017526534No Hit
CCCCAGTTTAGGGTCAAGAAAAATGTCGATGTGTTGCTCACTGTAAATAATT8280.17697526193621196No Hit
TGCTAGTTTAGGGAGGGCTCCCCCTGCCCAGGCCCCGGTGACGGTGACCGAG8210.17547909426283817No Hit
CGAGAGTTTAGGGAGAGCTTCTTGCTGACGAGGAGTCAGCGGCGCATCGCTC8200.1752653560237848No Hit
ACTGAGTTTAGGGCATGAAAACAAAAGCCCAAAGAAATAACTTTAAAACCAC8160.1744104030675712No Hit
TGTTAGTTTAGGGGGGGAATTCTCCAGATTACTTCCATTTCCACCCTTTTGA8090.17291423539419745No Hit
GGTCAGTTTAGGGAATGCATAATTAATGAATGCCTCCTATGTTTAAAGTGCT7770.16607461174448876No Hit
GTGTAGTTTAGGGGGTAAACGTGTCTGAATGAGAAGGCCAAGAAGATGAAAT7550.16137237048531405No Hit
ATGAAGTTTAGGGATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTT7530.16094489400720727No Hit
TTTGAGTTTAGGGGCTTCCTTTTACAGAAATGATTTTTATGTAATGTAAATG7200.15389153211844517No Hit
GCTAAGTTTAGGGCATGTTCTCACTTGTAAGTAGGAGCTGAACAATGAAAGA7150.15282284092317822No Hit
GCACAGTTTAGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG7000.14961676733737725No Hit
TTAAAGTTTAGGGAGAGGCCCACTCCTGCCACTTCTCGTCAACATAGTACTA6540.13978480834092105No Hit
CGCTAGTTTAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG6480.13850237890660066No Hit
CTTAAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC6450.13786116418944047No Hit
TCGCAGTTTAGGGCGTCTCCTTCGCCCCGCTGTCCGGAACCCTGGAGGAGAC6250.13358639940837255No Hit
TGTTAGTTTAGGGTTCTTACACATCAGGAGATGGCTCCGAGGTGGATTTCTA6220.13294518469121236No Hit
GGCTAGTTTAGGGTTATCTATATAACAAACCTGCACATGTACCCCTGAACTT6170.13187649349594538No Hit
GCATAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC6040.12909789638825123No Hit
ATTAAGTTTAGGGGGGCAACATAAAGAAATCCTGTCTCTACAGCAACAACAA6020.12867041991014444No Hit
GCTTAGTTTAGGGACAGTGAGCTATGATTGCGCTGATGCACTCCAGACTGGG5870.1254643463243435No Hit
GGTGAGTTTAGGGATAATATTGATACCTGGTGGCCCGCGATTGAAGCAGGTG5760.12311322569475616No Hit
GCAAAGTTTAGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTG5710.12204453449948917No Hit
AGAGAGTTTAGGGAATGAGTGAATAAAATGGAGGAAACAGCCATAACATGCT5620.12012089034800859No Hit
CACTAGTTTAGGGGGGAATTCCCCGGGCCAATTGCCTCTATAACTAGAGCTG5590.11947967563084841No Hit
CTAGAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC5460.11670107852315426No Hit
TTCAAGTTTAGGGGTTTCTAAAAATGATGGATATAAAACATTTCCTCAAAAC5380.11499117261072708No Hit
GGAAAGTTTAGGGGATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAAT5350.1143499578935669No Hit
GCATAGTTTAGGGGGAATTCCTGGAGATTGTCTCGTACGGTTAAGAGCCTCC5270.11264005198113973No Hit
TCCAAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5260.11242631374208635No Hit
AGACAGTTTAGGGTATCATATTGTTGGGATTAAAACATATATAGGCTATACA5200.11114388430776598No Hit
TGGTAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5130.1096477166343922No Hit
AGACAGTTTAGGGCTTTCCACTCTCTGGTGTATTATACTCTTCCCCTGTAAG5090.10879276367817863No Hit
GTTCAGTTTAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG5050.10793781072196502No Hit
GTACAGTTTAGGGGGAGAAGCCTCCAGAGGGACCTCTCTGTGAGAGGGAGAG5030.10751033424385822No Hit
GGCCAGTTTAGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAGCT4980.10644164304859124No Hit
CTCTAGTTTAAGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4940.10558669009237767No Hit
GGCTAGTTTAGGGGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT4940.10558669009237767No Hit
AATTAGTTTAGGGGCTGCTGGGCACAAGAGACTGGACTCTGCAGAGAGAGTC4910.10494547537521748No Hit
TACTAGTTTAGGGAACCAGAATCTACAACAGGTAGGTATTTTACTAAATGAT4800.10259435474563013No Hit
GCACAGTTTAGGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACAT4800.10259435474563013No Hit
TGTTAGTTTAGGGTAGTATCTTCTCTTAATATTATCTTGTAGGTATTCCATG4770.10195314002846995No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTGT206.307598E-446.00000443
ACTGCTA405.5988494E-946.00000434
CAGCTCA405.5988494E-946.00000433
CAGCCAC206.307598E-446.00000444
CAGCTAT206.307598E-446.00000441
TGGTTTT800.046.00000438
CTTTTAA405.5988494E-946.00000431
CTACAAC405.5988494E-946.00000423
TCCTCTG405.5988494E-946.00000420
GAGGCAC206.307598E-446.00000438
TGTTGCT1100.046.00000432
TTGTCGC405.5988494E-946.00000442
CCAATTG800.046.00000428
TTGTTCA206.307598E-446.00000419
GTGTCTC850.046.00000437
AATCTTT405.5988494E-946.00000428
TTCATGT206.307598E-446.00000432
CAAGAAG1050.046.00000439
TCCTCAA206.307598E-446.00000443
ACTAACG850.046.00000419
Produced by FastQC (version 0.11.5)