FastQCFastQC Report
Fri 17 Jun 2016
SRR1528127_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528127_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences467028
Sequences flagged as poor quality0
Sequence length52
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAGTTTAGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCG45780.9802410133867777No Hit
GGTCAGTTTAGGGGGCGATCACCAGCCCACCGCACAGAAGGATGAGGACCGC24260.5194549363207345No Hit
TGTGAGTTTAGGGACCTAACATTTAATTAAAAAGCCCTCTTAAAAGTGATTG21000.44965184100310907No Hit
ATTTAGTTTAGGGCTCCAGCCTGGGTGATCAAGTGAGACCCTGTCTCAAAAA17200.368286269773975No Hit
CGTGAGTTTAGGGGTGAAATACTGTACAGTATCCCCCTAAAATCAGGAACAT15000.32117988643079215No Hit
CTTAAGTTTAGGGAGGTCTAACACAAAGAAAAATTGTTCTGATCTACAGTTT14500.3104738902164324No Hit
ACTTAGTTTAGGGGTGCATTCTAGATTCAGTAAAATTCCAGTAGTAACAAGA14310.3064056116549757No Hit
TTATAGTTTAGGGCAGAAACTGCAATTCTCGGTTGTCAGGGAAGCAATGTTT14230.30469265226067815No Hit
TTAGAGTTTAGGGGACCAGGAGTTCAAAACCAGCCTGGCTAACATGGCGAGA13080.28006886096765077No Hit
GTATAGTTTAGGGGGTCAGGAGATCAAGACCATCCTGGCTAACACATGAAAC12130.25972746816036724No Hit
TCGCAGTTTAGGGTGTACCCTGATTTGACTTAGTGATCAAACTGTGCAGCAC11950.25587330952319776No Hit
CCATAGTTTAGGGGTTTGATATAATATGCATTTCGAGCCTTGGTTGCATTAC11580.24795087232457155No Hit
GATAAGTTTAGGGGGAGGCCAAGGCAGAGGATCACTTGAGCCCTTGAGTTCA11170.23917195542879655No Hit
GGCGAGTTTAGGGGATATTTTAAAGAACTGAATTTAATACCACCACATCCAT10780.23082127838159597No Hit
GGCGAGTTTAGGGTTGTGCACATGTACCCTAGAACTTAAAGTATAATAAAAA10720.2295365588358728No Hit
TCGTAGTTTAGGGGGCCGAGGCGGGCAGATCACAAGGTCTGGAGTTCGAGAC10420.22311296110725692No Hit
GACAAGTTTAGGGCTCTAGGACTCAGTAGAGACATTAGTAGACATGGCATTA10360.2218282415615338No Hit
ATTTAGTTTAGGGGTTCTATTTTTTCTATAGTCCTTACATTGGATTATCATT10150.21733172315150268No Hit
TGCAAGTTTAGAGACTACATACTGCACAGAACTTCTTTTCTATATATAAAGT10130.2169034833029283No Hit
CGGCAGTTTAGGGATCAGACTAACGACCACCAGGCCCAGCGACAGGGCACTG9630.20619748708856855No Hit
ACTTAGTTTAGGGCGGCACGAAGTGTCTTCCGATGCAGTGTCCAGTCATGAC9610.20576924723999418No Hit
GTGGAGTTTAGGGGTCATTTTGCGCCCCAGCACATCAAAATAACCGCTTAAC9500.203413928072835No Hit
TTTGAGTTTAGGGGTAATTAATTTAAAATTCCATTAAAATGAAAAAATAATT9230.19763269011708076No Hit
GTTCAGTTTAGGGAGCCTGCCCAACACAGTGAAAATGTGTCTCTACTAAAAA9060.19399265140419847No Hit
TGTTAGTTTAGGGGAACACGAAAGTCTGCAATGGGGAGACAGACACTAATCA9040.1935644115556241No Hit
CCCCAGTTTAGGGTCAAGAAAAATGTCGATGTGTTGCTCACTGTAAATAATT8530.18264429541697713No Hit
ACTGAGTTTAGGGCATGAAAACAAAAGCCCAAAGAAATAACTTTAAAACCAC8510.18221605556840273No Hit
TTTTAGTTTAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGC8280.17729129730979726No Hit
GCTTAGTTTAGGGGAGGGATGGAGTCACAATCACACAGGGGAAAAGGTTGAA8190.17536421799121252No Hit
ATATAGTTTAGGGTTCCTGCTTGGTCTTGGCGTTACGCAACCGAAGCTGGAT7990.1710818195054686No Hit
GGTCAGTTTAGGGAATGCATAATTAATGAATGCCTCCTATGTTTAAAGTGCT7860.1682982604897351No Hit
TGTTAGTTTAGGGGGGGAATTCTCCAGATTACTTCCATTTCCACCCTTTTGA7790.16679942101972473No Hit
CGAGAGTTTAGGGAGAGCTTCTTGCTGACGAGGAGTCAGCGGCGCATCGCTC7620.1631593823068424No Hit
TGCTAGTTTAGGGAGGGCTCCCCCTGCCCAGGCCCCGGTGACGGTGACCGAG7530.16123230298825766No Hit
ATGAAGTTTAGGGATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTT7460.1597334635182473No Hit
GCTAAGTTTAGGGCATGTTCTCACTTGTAAGTAGGAGCTGAACAATGAAAGA7170.15352398571391865No Hit
GCACAGTTTAGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAG7080.1515969063953339No Hit
CTTAAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC7020.15031218684961073No Hit
GTGTAGTTTAGGGGGTAAACGTGTCTGAATGAGAAGGCCAAGAAGATGAAAT7010.15009806692532354No Hit
CGCTAGTTTAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAG6860.14688626806101562No Hit
TTTGAGTTTAGGGGCTTCCTTTTACAGAAATGATTTTTATGTAATGTAAATG6710.1436744691967077No Hit
TTAAAGTTTAGGGAGAGGCCCACTCCTGCCACTTCTCGTCAACATAGTACTA6470.13853559101381502No Hit
GGCTAGTTTAGGGTTATCTATATAACAAACCTGCACATGTACCCCTGAACTT6220.13318259290663514No Hit
TGTTAGTTTAGGGTTCTTACACATCAGGAGATGGCTCCGAGGTGGATTTCTA5960.12761547487516808No Hit
GCAAAGTTTAGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTG5830.12483191585943454No Hit
GCATAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC5820.12461779593514735No Hit
TCGCAGTTTAGGGCGTCTCCTTCGCCCCGCTGTCCGGAACCCTGGAGGAGAC5800.12418955608657298No Hit
GGTGAGTTTAGGGATAATATTGATACCTGGTGGCCCGCGATTGAAGCAGGTG5660.12119187714655223No Hit
TCCAAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5630.12054951737369066No Hit
GCTTAGTTTAGGGACAGTGAGCTATGATTGCGCTGATGCACTCCAGACTGGG5610.12012127752511627No Hit
TTCAAGTTTAGGGGTTTCTAAAAATGATGGATATAAAACATTTCCTCAAAAC5540.1186224380551059No Hit
GGAAAGTTTAGGGGATTACCACATCACCCGCCAGTGGCAGGAACGTGGTAAT5410.11583887903937237No Hit
TGGTAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAA5380.11519651926651078No Hit
CACTAGTTTAGGGGGGAATTCCCCGGGCCAATTGCCTCTATAACTAGAGCTG5370.11498239934222358No Hit
GTACAGTTTAGGGGGAGAAGCCTCCAGAGGGACCTCTCTGTGAGAGGGAGAG5310.11369767979650042No Hit
CTAGAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTC5160.1104858809321925No Hit
ATTAAGTTTAGGGGGGCAACATAAAGAAATCCTGTCTCTACAGCAACAACAA5140.11005764108361811No Hit
GGCTAGTTTAGGGGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCT5070.10855880161360774No Hit
AGAGAGTTTAGGGAATGAGTGAATAAAATGGAGGAAACAGCCATAACATGCT5060.10834468168932054No Hit
GGCCAGTTTAGGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAGCT5020.10748820199217178No Hit
AGACAGTTTAGGGTATCATATTGTTGGGATTAAAACATATATAGGCTATACA4940.10577524259787421No Hit
GGAAAGTTTAGGGGATATAAGATCAATCAAGTGCACACAGAACAGGATATAG4890.10470464297643824No Hit
GTTCAGTTTAGGGGGGAATTCAGATAAAACGAATAGCTCGTAACCAAACATG4820.10320580350642787No Hit
AGACAGTTTAGGGCTTTCCACTCTCTGGTGTATTATACTCTTCCCCTGTAAG4780.10234932380927911No Hit
TGTTAGTTTAGGGTAGTATCTTCTCTTAATATTATCTTGTAGGTATTCCATG4770.10213520388499191No Hit
GAACAGTTTAGGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAG4730.10127872418784313No Hit
TACTAGTTTAGGGAACCAGAATCTACAACAGGTAGGTATTTTACTAAATGAT4710.10085048433926874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGTG351.0178337E-746.00000431
CTTTTGT351.0178337E-746.00000430
CTTCATG405.5988494E-946.00000432
GGAGATT351.0178337E-746.00000423
ACTGCTA405.5988494E-946.00000434
ACTGTTC206.307586E-446.00000422
TGGTTTT800.046.00000438
CTACAAC351.0178337E-746.00000423
GAGGCGG800.046.00000418
GCGGGCA800.046.00000421
TCCTTTA405.5988494E-946.00000434
TGTGACA405.5988494E-946.00000436
TTGTCCA206.307586E-446.00000423
CCGGATT351.0178337E-746.00000443
AACGTGT800.046.00000418
TTGTTAT405.5988494E-946.00000442
CAAGACC1400.046.00000425
CCGTGTC206.307586E-446.00000419
TCCTCAA700.046.00000443
GGTGACC1050.046.00000443
Produced by FastQC (version 0.11.5)