FastQCFastQC Report
Fri 17 Jun 2016
SRR1528126_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528126_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences425824
Sequences flagged as poor quality0
Sequence length59
%GC36

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TACGAGTTTAGGGGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCCGAAGCTGG37200.8736003607124071No Hit
GGTCAGTTTAGGGGGCGATCACCAGCCCACCGCACAGAAGGATGAGGACCGCCAGCCAG18410.43233824302998425No Hit
TGTGAGTTTAGGGACCTAACATTTAATTAAAAAGCCCTCTTAAAAGTGATTGTTGGCCG15760.37010595926955736No Hit
ATTTAGTTTAGGGCTCCAGCCTGGGTGATCAAGTGAGACCCTGTCTCAAAAAAATAAAA14460.3395769144059518No Hit
ACTTAGTTTAGGGGTGCATTCTAGATTCAGTAAAATTCCAGTAGTAACAAGATTTTGAA13000.3052904486360562No Hit
CGTGAGTTTAGGGGTGAAATACTGTACAGTATCCCCCTAAAATCAGGAACATAGTAAGG12950.3041162546028406No Hit
CTTAAGTTTAGGGAGGTCTAACACAAAGAAAAATTGTTCTGATCTACAGTTTGAGATAT12310.2890865709776809No Hit
TTAGAGTTTAGGGGACCAGGAGTTCAAAACCAGCCTGGCTAACATGGCGAGACCCCATC11080.26020139776057716No Hit
CCATAGTTTAGGGGTTTGATATAATATGCATTTCGAGCCTTGGTTGCATTACTTCCATT10910.2562091380476441No Hit
GTATAGTTTAGGGGGTCAGGAGATCAAGACCATCCTGGCTAACACATGAAACCCCACCT10320.2423536484557No Hit
TTATAGTTTAGGGCAGAAACTGCAATTCTCGGTTGTCAGGGAAGCAATGTTTTCAGCTA10220.2400052603892688No Hit
GACAAGTTTAGGGCTCTAGGACTCAGTAGAGACATTAGTAGACATGGCATTATTAGTAG10210.23977042158262568No Hit
GATAAGTTTAGGGGGAGGCCAAGGCAGAGGATCACTTGAGCCCTTGAGTTCAAGGCCAG10170.2388310663560532No Hit
TCGCAGTTTAGGGTGTACCCTGATTTGACTTAGTGATCAAACTGTGCAGCACCTTGTTC10040.23577816186969264No Hit
ATTTAGTTTAGGGGTTCTATTTTTTCTATAGTCCTTACATTGGATTATCATTTAATTAC9480.22262718869767792No Hit
GTGGAGTTTAGGGGTCATTTTGCGCCCCAGCACATCAAAATAACCGCTTAACTCCACAC9440.22168783347110543No Hit
GGCGAGTTTAGGGGATATTTTAAAGAACTGAATTTAATACCACCACATCCATTATTCAG8930.2097110543323063No Hit
TGCAAGTTTAGAGACTACATACTGCACAGAACTTCTTTTCTATATATAAAGTTTGAAGG8460.19867363042007968No Hit
TTTGAGTTTAGGGGTAATTAATTTAAAATTCCATTAAAATGAAAAAATAATTTTTACTG8190.1923329826407154No Hit
CCCCAGTTTAGGGTCAAGAAAAATGTCGATGTGTTGCTCACTGTAAATAATTTGCCATT8160.19162846622078605No Hit
ACTGAGTTTAGGGCATGAAAACAAAAGCCCAAAGAAATAACTTTAAAACCACCAAAACA7820.18364394679491997No Hit
ACTTAGTTTAGGGCGGCACGAAGTGTCTTCCGATGCAGTGTCCAGTCATGACCTCGATC7740.181765236341775No Hit
GTTCAGTTTAGGGAGCCTGCCCAACACAGTGAAAATGTGTCTCTACTAAAAATACAAAA7610.17871233185541444No Hit
TCGTAGTTTAGGGGGCCGAGGCGGGCAGATCACAAGGTCTGGAGTTCGAGACCAGCCTG7410.17401555572255203No Hit
GCTAAGTTTAGGGCATGTTCTCACTTGTAAGTAGGAGCTGAACAATGAAAGACACTTGG7120.16720523032990156No Hit
TGTTAGTTTAGGGGAACACGAAAGTCTGCAATGGGGAGACAGACACTAATCAGATGGTC7060.16579619749004282No Hit
CGGCAGTTTAGGGATCAGACTAACGACCACCAGGCCCAGCGACAGGGCACTGTGCGCCA6980.16391748703689787No Hit
GGTCAGTTTAGGGAATGCATAATTAATGAATGCCTCCTATGTTTAAAGTGCTGCTTAGC6960.16344780942361162No Hit
TTTTAGTTTAGGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGT6830.1603949049372511No Hit
ATATAGTTTAGGGTTCCTGCTTGGTCTTGGCGTTACGCAACCGAAGCTGGATAAAGTGA6770.15898587209739234No Hit
TTTGAGTTTAGGGGCTTCCTTTTACAGAAATGATTTTTATGTAATGTAAATGGCAGGTA6440.15123619147816939No Hit
CGAGAGTTTAGGGAGAGCTTCTTGCTGACGAGGAGTCAGCGGCGCATCGCTCGGAAGCA6280.14747877057187947No Hit
GCTTAGTTTAGGGGAGGGATGGAGTCACAATCACACAGGGGAAAAGGTTGAAGAAAAGG6210.14583489892537763No Hit
TTAAAGTTTAGGGAGAGGCCCACTCCTGCCACTTCTCGTCAACATAGTACTAGAAGTCC6050.1420774780190877No Hit
TGCTAGTTTAGGGAGGGCTCCCCCTGCCCAGGCCCCGGTGACGGTGACCGAGGGCTCCC6020.14137296159915833No Hit
GCACAGTTTAGGGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCAT5960.1399639287592996No Hit
GTGTAGTTTAGGGGGTAAACGTGTCTGAATGAGAAGGCCAAGAAGATGAAATCAGGAAT5920.13902457353272712No Hit
GGCGAGTTTAGGGTTGTGCACATGTACCCTAGAACTTAAAGTATAATAAAAAAAAAATA5900.1385548959194409No Hit
ATTAAGTTTAGGGGGGCAACATAAAGAAATCCTGTCTCTACAGCAACAACAACAACATA5830.13691102427293905No Hit
TGTTAGTTTAGGGGGGGAATTCTCCAGATTACTTCCATTTCCACCCTTTTGAGCAATGA5600.1315097317201473No Hit
ATGAAGTTTAGGGATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCA5410.12704779439392802No Hit
CTTAAGTTTAGGGGGGAATTCACACCGGCGCACGCCACAGGCGTCATACTTCCCAAGAA5410.12704779439392802No Hit
CGCTAGTTTAGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTGG5320.12493424513413992No Hit
GCTTAGTTTAGGGACAGTGAGCTATGATTGCGCTGATGCACTCCAGACTGGGTGACAGA5200.12211617945442248No Hit
TGTTAGTTTAGGGTTCTTACACATCAGGAGATGGCTCCGAGGTGGATTTCTACAAAGAA4810.1129574659953408No Hit
TCCAAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG4740.11131359434883896No Hit
GGAAAGTTTAGGGGATATAAGATCAATCAAGTGCACACAGAACAGGATATAGAAGTCCT4730.11107875554219585No Hit
TCGCAGTTTAGGGCGTCTCCTTCGCCCCGCTGTCCGGAACCCTGGAGGAGACCTACCTG4550.10685165702261969No Hit
GCAAAGTTTAGGGGGGAATTCCTCTGTGTCATGATCGTGAGTTGTCGCAGTGTCTGTAC4480.10520778537611783No Hit
TGGTAGTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGGCATTG4410.103563913729616No Hit
CTTTAGTTTAGGGGGTACTCACCATCCAGATGATATAAGAGGAAAACCACATAAACATA4370.10262455850304351No Hit
AGAGAGTTTAGGGAATGAGTGAATAAAATGGAGGAAACAGCCATAACATGCTCCTAGTC4340.10192004208311414No Hit
GTACAGTTTAGGGGGAGAAGCCTCCAGAGGGACCTCTCTGTGAGAGGGAGAGAGGCAGC4270.10027617043661231No Hit
TTCAAGTTTAGGGGTTTCTAAAAATGATGGATATAAAACATTTCCTCAAAACTGATGTT4260.10004133162996919No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATACTT1950.053.00000445
AATTCAC1950.053.00000417
GCGTCAT1950.053.00000441
CGTCATA1950.053.00000442
TGGCTCA4950.053.00000416
CCTCTTA1950.053.00000436
AGCACTT4950.053.00000436
CTTGTTG401.5970727E-953.030
CTTGTTC1450.053.053
TCACGCG150.006693385553.025
CTTAATC203.1359968E-453.052
CTTAATA353.335481E-853.025
TTGATTC600.053.039
TCTGCCC401.5970727E-953.016
GTTTGTG203.1359968E-453.040
GTGCATT1150.053.014
ATTTAGC150.006693385553.024
AACGTTT203.1359968E-453.027
TGTCATG600.053.027
TTGATAC600.053.020
Produced by FastQC (version 0.11.5)