FastQCFastQC Report
Fri 17 Jun 2016
SRR1528125_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528125_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2446901
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTACCGGGACATGATCTCACAAAATATCATTTCAAAACCAATTATTA35700.14589883285020522No Hit
TCTTTCTACCGGGGAGCATCCACAGCGCTCACTACCCTGGCCTGGCCCCTGT35130.14356935568705068No Hit
AGACTCTACCGGGATACTACCTTCTGAAGAACCCTGCTTTCTTCTGAAAAAT34890.1425885231973014No Hit
AAAATCTACCGGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGGCCGAG31740.12971509676934212No Hit
TACTTCTACCGGGGGACCATCTTCGACAGTGACCTTATAGACAGCACCATGC31670.12942902062649858No Hit
TTCTTCTACCGGGCAGGAGGCTGAGAATCACTTAAACCCGGGAGACAGAGGT31490.1286933962591866No Hit
TAACTCTACCGGGGGAGAGATAGCTCAGAAGTTAAGAGCACTGACTGTTCTT30340.12399357391247132No Hit
GTTATCTACCGGGGCAGAGTTCTATCTACGGGGGAGAGATGGCTCAGTGGCT27500.11238705611710485No Hit
CGGCTCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27110.11079320332126229No Hit
ATGCTCTACCGGGGGCCCCGCAGCGTCGCGCCCGCGCACGAGTGGTCGGCAC25990.10621598503576565No Hit
TATGTCTACCGGGGGAGAGATGGCTCAGTGGCTAAAAGCACTGACTGCTCTT25470.10409084797464221No Hit
CTGCTCTACCGGATGAAGCAGACTGTTTACTTATTAAAACTTATTTATTGTA25290.10335522360733025No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCC1050.046.00000443
ATAGGCG1050.046.00000414
CAAAACT2100.046.00000414
CGGTTGT1050.046.00000441
CTAGTAC2100.046.00000428
CGCTAGT2100.046.00000426
CTCGACG1050.046.00000420
TATAGAC3250.046.00000436
TAGGCGG1050.046.00000415
TTATTAC1050.046.00000444
TAATCAC253.418296E-546.036
TCACGCT551.8189894E-1246.031
GTCATTG206.3128583E-446.032
TCGCCAC1500.046.042
AACGTTA551.8189894E-1246.029
CTGCTAA650.046.037
CGTGGTT501.6370905E-1146.019
TTTAGAC405.6152203E-946.026
CTAGACG950.046.029
AACGTAC453.110472E-1046.014
Produced by FastQC (version 0.11.5)