FastQCFastQC Report
Fri 17 Jun 2016
SRR1528124_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528124_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2439834
Sequences flagged as poor quality0
Sequence length52
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTACCGGGACATGATCTCACAAAATATCATTTCAAAACCAATTATTA35840.1468952395941691No Hit
TCTTTCTACCGGGGAGCATCCACAGCGCTCACTACCCTGGCCTGGCCCCTGT33490.137263436774797No Hit
AGACTCTACCGGGATACTACCTTCTGAAGAACCCTGCTTTCTTCTGAAAAAT33310.13652568166522805No Hit
TACTTCTACCGGGGGACCATCTTCGACAGTGACCTTATAGACAGCACCATGC31270.1281644570901135No Hit
TTCTTCTACCGGGCAGGAGGCTGAGAATCACTTAAACCCGGGAGACAGAGGT31070.12734472919059248No Hit
AAAATCTACCGGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGGCCGAG30790.12619711013126303No Hit
TAACTCTACCGGGGGAGAGATAGCTCAGAAGTTAAGAGCACTGACTGTTCTT30640.12558231420662225No Hit
TATGTCTACCGGGGGAGAGATGGCTCAGTGGCTAAAAGCACTGACTGCTCTT26820.10992551132577053No Hit
CGGCTCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26770.10972057935089026No Hit
ATGCTCTACCGGGGGCCCCGCAGCGTCGCGCCCGCGCACGAGTGGTCGGCAC26120.10705646367744691No Hit
GTTATCTACCGGGGCAGAGTTCTATCTACGGGGGAGAGATGGCTCAGTGGCT25990.10652364054275823No Hit
CTGCTCTACCGGATGAAGCAGACTGTTTACTTATTAAAACTTATTTATTGTA25050.10267091941500937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGT700.046.00000445
GTACCGC1100.046.00000416
AATCATC1500.046.00000428
ATCTATG2200.046.00000414
ATCTATA351.0201984E-746.00000417
CTACTAA351.0201984E-746.00000419
GATAAAC351.0201984E-746.00000442
TACTTAT2800.046.00000428
TACTTAG700.046.00000437
GATCGCG1100.046.00000423
CAAGTAC750.046.00000434
CGACGTT700.046.00000434
TGCCACG700.046.00000435
CAACGAC351.0201984E-746.00000428
ACGTTAG700.046.00000436
GCGGTAC700.046.00000444
TTTGTCG551.8189894E-1246.00000425
CTTCACA551.8189894E-1246.00000426
CACATTA351.0201984E-746.00000441
ATAATAG700.046.00000414
Produced by FastQC (version 0.11.5)