##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1528124_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2439834
Sequences flagged as poor quality	0
Sequence length	52
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148800287232657	31.0	31.0	34.0	30.0	34.0
2	31.81960084169661	31.0	31.0	34.0	30.0	34.0
3	32.028759743490745	33.0	31.0	34.0	30.0	34.0
4	35.79311830231073	37.0	35.0	37.0	35.0	37.0
5	35.69215118733488	37.0	35.0	37.0	33.0	37.0
6	35.702810519076294	37.0	35.0	37.0	33.0	37.0
7	35.70165962110537	37.0	35.0	37.0	33.0	37.0
8	35.68369364473157	37.0	35.0	37.0	33.0	37.0
9	37.30464900480934	39.0	37.0	39.0	33.0	39.0
10	37.28763514239083	39.0	37.0	39.0	33.0	39.0
11	37.40385452452913	39.0	37.0	39.0	35.0	39.0
12	37.28685476143049	39.0	37.0	39.0	35.0	39.0
13	37.226579759114756	39.0	37.0	39.0	35.0	39.0
14	37.66205528736791	40.0	38.0	40.0	32.0	40.0
15	37.12180500804563	39.0	37.0	40.0	31.0	40.0
16	36.58081492429403	38.0	35.0	40.0	31.0	40.0
17	36.40842696675266	39.0	35.0	40.0	31.0	40.0
18	36.46665797755093	39.0	35.0	40.0	30.0	40.0
19	36.580303823948675	39.0	35.0	40.0	31.0	40.0
20	36.60877994158619	39.0	35.0	40.0	31.0	40.0
21	36.62069673592548	39.0	35.0	40.0	30.0	40.0
22	36.68312106479375	39.0	35.0	40.0	31.0	40.0
23	36.75701912507162	39.0	35.0	40.0	31.0	40.0
24	36.78264627839435	39.0	35.0	40.0	31.0	40.0
25	36.77576630213367	39.0	35.0	40.0	31.0	40.0
26	36.40404142249022	39.0	35.0	40.0	30.0	40.0
27	36.06593932210142	38.0	35.0	40.0	30.0	40.0
28	35.914503609671804	38.0	35.0	40.0	30.0	40.0
29	35.93584071703239	38.0	35.0	40.0	30.0	40.0
30	35.73319455339995	38.0	35.0	40.0	30.0	40.0
31	35.72562067747232	38.0	35.0	40.0	30.0	40.0
32	35.7325854955706	38.0	35.0	40.0	30.0	40.0
33	35.701664129608815	38.0	35.0	40.0	30.0	40.0
34	35.59068649752401	38.0	35.0	40.0	29.0	40.0
35	35.286364974010525	37.0	34.0	40.0	28.0	40.0
36	35.11075753514378	37.0	34.0	40.0	27.0	40.0
37	34.9144302440248	37.0	34.0	40.0	26.0	40.0
38	34.58600503148985	37.0	33.0	40.0	25.0	40.0
39	34.23703128983365	36.0	33.0	40.0	24.0	40.0
40	33.82899451356117	35.0	33.0	40.0	23.0	40.0
41	33.471721026922324	35.0	33.0	39.0	21.0	40.0
42	33.11223919332217	35.0	33.0	39.0	19.0	40.0
43	32.76128785810838	35.0	33.0	39.0	17.0	40.0
44	32.335142472807576	35.0	32.0	39.0	15.0	40.0
45	32.121866897502045	35.0	32.0	39.0	12.0	40.0
46	31.80550766978409	35.0	31.0	38.0	10.0	40.0
47	31.545887548087286	35.0	31.0	38.0	10.0	40.0
48	31.258271259438143	35.0	31.0	38.0	10.0	40.0
49	30.890881510791306	35.0	30.0	37.0	10.0	40.0
50	30.669950496632147	35.0	29.0	37.0	9.0	40.0
51	30.375381685803216	35.0	29.0	37.0	9.0	40.0
52	29.37350327932146	34.0	26.0	36.0	8.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	4.0
14	14.0
15	34.0
16	154.0
17	448.0
18	1129.0
19	2385.0
20	4439.0
21	7351.0
22	11207.0
23	16193.0
24	22414.0
25	30412.0
26	40102.0
27	52437.0
28	67399.0
29	83309.0
30	96741.0
31	101376.0
32	105593.0
33	121580.0
34	165569.0
35	258181.0
36	273525.0
37	407433.0
38	477057.0
39	93345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.016728187245526	22.427386453340677	35.728004446204125	12.827880913209668
2	24.4489174263495	23.1745684337541	33.94054677490354	18.435967364992862
3	17.449096946759493	24.294275758104856	37.403897150379905	20.85273014475575
4	20.120959048853322	23.38786163320947	35.09062501793155	21.400554300005656
5	0.0	0.0	100.0	0.0
6	0.0	0.0	0.0	100.0
7	0.0	0.0	100.0	0.0
8	0.0	100.0	0.0	0.0
9	0.0	0.0	0.0	100.0
10	0.0	0.0	0.0	100.0
11	98.48538876005499	0.9921576631852823	0.25038588690869956	0.27206768985103086
12	97.1326327938704	2.471848494610699	0.21214558039604334	0.18337313112285508
13	92.95333207095237	5.234577434366437	1.0774913375254218	0.7345991571557737
14	48.26676732925273	34.845526375974764	6.748901769546617	10.138804525225895
15	34.13625681091418	32.48589863080849	16.75048384439269	16.627360713884634
16	35.4482313140976	26.218832920600338	21.685163826719357	16.647771938582707
17	23.473605171499372	25.43791913712162	25.40463818440107	25.683837506977937
18	22.398654990462465	31.595141308793963	27.360836843818063	18.645366856925513
19	26.947366091299653	29.92174877471172	26.138622545632202	16.99226258835642
20	23.35798255127193	32.626031115231605	25.615759104922713	18.400227228573748
21	26.12567084481977	30.90681579156615	20.09308830026961	22.87442506334447
22	26.493646698914763	32.288426179813875	22.11990651823034	19.09802060304103
23	22.242537811998687	35.374455803140705	21.12643729040582	21.256569094454786
24	22.597766897256125	37.056127589008106	21.60261722723759	18.74348828649818
25	23.60836843818063	34.11240272903812	21.98432352364956	20.294905309131686
26	22.053713490344016	33.20541479461308	19.191469583586425	25.549402131456482
27	24.272675927952474	36.625811428154535	17.573449669116833	21.52806297477615
28	25.062442772746014	33.779470242647655	17.45897466794872	23.699112316657608
29	25.454190735927117	32.94031479190798	15.234315121438591	26.37117935072632
30	27.39399483735369	34.45394235837356	17.798628923115263	20.35343388115749
31	22.524483223038942	38.03541552417091	15.285261210393822	24.154840042396327
32	21.91464665219027	41.88280842057288	16.88885391383184	19.31369101340501
33	25.81134618174843	39.226439175779994	17.173545413335496	17.78866922913608
34	21.038562459577168	42.67614108172933	16.5832593528904	19.7020371058031
35	24.991823214202277	40.42402065058525	17.713459194355025	16.870696940857453
36	23.51287013788643	39.19635516186757	20.191455648212134	17.099319052033866
37	23.058085099232162	40.5392743932579	18.302761581320695	18.09987892618924
38	20.854656505319625	41.53241572992261	21.73340481360617	15.879522951151595
39	21.335549877573637	42.7292594496183	19.42414934786547	16.5110413249426
40	22.2158146824743	43.094530201644865	18.339239472849382	16.35041564303145
41	23.980319972588298	41.70025501734954	16.698554081958033	17.620870928104125
42	22.22339716554487	41.76542338536146	15.966946931635512	20.044232517458155
43	25.06916454152209	38.63250532618203	18.32641073122188	17.971919401074008
44	22.085150055290647	40.88679803625984	16.564733502361225	20.463318406088284
45	21.74639750081358	36.53428880817302	18.250872805281016	23.468440885732388
46	22.13650600819564	36.44219237866182	16.364105098953456	25.05719651418908
47	24.051595313451653	37.33950752387253	17.679440486524904	20.929456676150917
48	26.358719486653598	35.47802842324519	18.046678585510325	20.11657350459089
49	23.844368100452737	38.20952573002918	19.384720435898508	18.56138573361958
50	22.91131281882292	39.86799921634013	16.214586730080818	21.006101234756137
51	20.762068239068725	40.21654751921647	19.889139998868778	19.13224424284603
52	23.896133917307488	36.60216227825336	20.15805173630665	19.343652068132506
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	5.5
6	11.0
7	722.0
8	1433.0
9	2102.5
10	2772.0
11	4468.5
12	6165.0
13	7691.5
14	11956.5
15	14695.0
16	15506.0
17	16317.0
18	16718.0
19	17119.0
20	17516.5
21	17914.0
22	20636.0
23	23358.0
24	29630.0
25	35902.0
26	44968.5
27	54035.0
28	63735.5
29	73436.0
30	76919.0
31	80402.0
32	84351.0
33	88300.0
34	84712.0
35	81124.0
36	77112.5
37	73101.0
38	68987.0
39	65008.5
40	65144.0
41	71940.5
42	78737.0
43	86723.0
44	94709.0
45	100260.5
46	105812.0
47	115261.5
48	124711.0
49	123929.0
50	123147.0
51	139376.5
52	155606.0
53	160399.0
54	165192.0
55	163831.0
56	162470.0
57	167452.5
58	172435.0
59	158450.0
60	144465.0
61	133611.0
62	122757.0
63	105632.5
64	74542.5
65	60577.0
66	51352.0
67	42127.0
68	38832.0
69	35537.0
70	27279.5
71	19022.0
72	15053.0
73	11084.0
74	6279.5
75	1475.0
76	3509.0
77	5543.0
78	3025.0
79	507.0
80	281.0
81	55.0
82	44.5
83	34.0
84	18.5
85	3.0
86	2.0
87	1.0
88	0.5
89	0.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
52	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
52	2439834.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.1742234031316
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.24758393095405	20.849683619272263
2	5.409772704151732	2.6155410782065465
3	1.8048393971669938	1.308917723816648
4	0.9676107584383747	0.9356495456705151
5	0.6446376381230013	0.7791807139026268
6	0.492646352890972	0.7145605796114618
7	0.37443746127491395	0.6336214387552843
8	0.3113128621878478	0.6020597343037882
9	0.2547768649475792	0.5543129566073041
>10	2.8264382593167	14.425100669518237
>50	0.2590156346655657	4.188379869165817
>100	0.24384493449415928	15.324034074888202
>500	0.11143729914197192	19.419824404122913
>1k	0.05164590224620161	17.6491335921584
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ACGTTCTACCGGGACATGATCTCACAAAATATCATTTCAAAACCAATTATTA	3584	0.1468952395941691	No Hit
TCTTTCTACCGGGGAGCATCCACAGCGCTCACTACCCTGGCCTGGCCCCTGT	3349	0.137263436774797	No Hit
AGACTCTACCGGGATACTACCTTCTGAAGAACCCTGCTTTCTTCTGAAAAAT	3331	0.13652568166522805	No Hit
TACTTCTACCGGGGGACCATCTTCGACAGTGACCTTATAGACAGCACCATGC	3127	0.1281644570901135	No Hit
TTCTTCTACCGGGCAGGAGGCTGAGAATCACTTAAACCCGGGAGACAGAGGT	3107	0.12734472919059248	No Hit
AAAATCTACCGGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGGCCGAG	3079	0.12619711013126303	No Hit
TAACTCTACCGGGGGAGAGATAGCTCAGAAGTTAAGAGCACTGACTGTTCTT	3064	0.12558231420662225	No Hit
TATGTCTACCGGGGGAGAGATGGCTCAGTGGCTAAAAGCACTGACTGCTCTT	2682	0.10992551132577053	No Hit
CGGCTCTACCGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2677	0.10972057935089026	No Hit
ATGCTCTACCGGGGGCCCCGCAGCGTCGCGCCCGCGCACGAGTGGTCGGCAC	2612	0.10705646367744691	No Hit
GTTATCTACCGGGGCAGAGTTCTATCTACGGGGGAGAGATGGCTCAGTGGCT	2599	0.10652364054275823	No Hit
CTGCTCTACCGGATGAAGCAGACTGTTTACTTATTAAAACTTATTTATTGTA	2505	0.10267091941500937	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.018812755294007708	0.0	0.0	0.0	0.0
12	0.04947057873609434	0.0	0.0	0.0	0.0
13	0.06111071490929301	0.0	0.0	0.0	0.0
14	0.10685153170256664	0.0	0.0	0.0	0.0
15	0.1902998318738078	0.0	0.0	0.0	0.0
16	0.28128962872064245	0.0	0.0	0.0	0.0
17	0.3560488131569607	0.0	0.0	0.0	0.0
18	0.45388333796479596	0.0	0.0	0.0	0.0
19	0.5524146314872241	0.0	0.0	0.0	0.0
20	0.6499212651352511	0.0	0.0	0.0	0.0
21	0.7564858920729853	0.0	0.0	0.0	0.0
22	0.8666573217686121	0.0	0.0	0.0	0.0
23	0.9614178669532435	0.0	0.0	0.0	0.0
24	1.0673676979663371	0.0	0.0	0.0	0.0
25	1.1837280733033477	0.0	0.0	0.0	0.0
26	1.3073020541561433	0.0	0.0	0.0	0.0
27	1.4222688920639683	0.0	0.0	0.0	0.0
28	1.541867192604087	0.0	0.0	0.0	0.0
29	1.6776551191597462	0.0	0.0	0.0	0.0
30	1.8437729779976835	0.0	0.0	0.0	0.0
31	1.9946029115095536	0.0	0.0	0.0	0.0
32	2.1122748514857976	0.0	0.0	0.0	0.0
33	2.205477913661339	0.0	0.0	0.0	0.0
34	2.2914263839261197	0.0	0.0	0.0	0.0
35	2.372456486793774	0.0	0.0	0.0	0.0
36	2.4577491747389373	0.0	0.0	0.0	0.0
37	2.542386080364484	0.0	0.0	0.0	0.0
38	2.6323922037318934	0.0	0.0	0.0	0.0
39	2.7340794496674774	0.0	0.0	0.0	0.0
40	2.831995947265265	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGT	70	0.0	46.000004	45
GTACCGC	110	0.0	46.000004	16
AATCATC	150	0.0	46.000004	28
ATCTATG	220	0.0	46.000004	14
ATCTATA	35	1.0201984E-7	46.000004	17
CTACTAA	35	1.0201984E-7	46.000004	19
GATAAAC	35	1.0201984E-7	46.000004	42
TACTTAT	280	0.0	46.000004	28
TACTTAG	70	0.0	46.000004	37
GATCGCG	110	0.0	46.000004	23
CAAGTAC	75	0.0	46.000004	34
CGACGTT	70	0.0	46.000004	34
TGCCACG	70	0.0	46.000004	35
CAACGAC	35	1.0201984E-7	46.000004	28
ACGTTAG	70	0.0	46.000004	36
GCGGTAC	70	0.0	46.000004	44
TTTGTCG	55	1.8189894E-12	46.000004	25
CTTCACA	55	1.8189894E-12	46.000004	26
CACATTA	35	1.0201984E-7	46.000004	41
ATAATAG	70	0.0	46.000004	14
>>END_MODULE