FastQCFastQC Report
Fri 17 Jun 2016
SRR1528123_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1528123_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2275599
Sequences flagged as poor quality0
Sequence length59
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTTCTACCGGGACATGATCTCACAAAATATCATTTCAAAACCAATTATTACACAGTT31700.1393039810616897No Hit
AGACTCTACCGGGATACTACCTTCTGAAGAACCCTGCTTTCTTCTGAAAAATCTGAGGA31610.13890848080000034No Hit
TAACTCTACCGGGGGAGAGATAGCTCAGAAGTTAAGAGCACTGACTGTTCTTCATAAGG28660.12594486111129422No Hit
TCTTTCTACCGGGGAGCATCCACAGCGCTCACTACCCTGGCCTGGCCCCTGTGAAAGAG27650.1215064692856694No Hit
TTCTTCTACCGGGCAGGAGGCTGAGAATCACTTAAACCCGGGAGACAGAGGTCGCAGTT26040.11443140904878232No Hit
AAAATCTACCGGGTGGCTCAGGCCTGTAATCCCAGCACTTTGGGAGGCCGAGGTGGGTA25650.11271757458146184No Hit
TACTTCTACCGGGGGACCATCTTCGACAGTGACCTTATAGACAGCACCATGCTGGGGGG24110.10595012565922203No Hit
GTTATCTACCGGGGCAGAGTTCTATCTACGGGGGAGAGATGGCTCAGTGGCTAAAAGCA23830.10471968040063298No Hit
TATGTCTACCGGGGGAGAGATGGCTCAGTGGCTAAAAGCACTGACTGCTCTTCCAGAGG23290.10234667883049695No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA2150.053.00000418
AACGTTA503.6379788E-1253.029
CTTAACA150.00669710653.052
TTAGGTG203.1388993E-453.036
TACTTTA353.34403E-853.048
ATTAGGC150.00669710653.041
ATAGGTA353.34403E-853.052
GATAACG503.6379788E-1253.018
CAACGTC800.053.023
CGACGAA150.00669710653.032
TCCGATA700.053.025
ACGTTCC550.053.043
ACGTTAT503.6379788E-1253.030
ACGTTAG457.6397555E-1153.036
CGTGCGA550.053.049
TTAGCCA1900.053.031
CGTGCAC251.4791944E-553.047
TCCGAAC1050.053.030
CGCATTA150.00669710653.021
AACTTAT2100.053.038